cip m & b presentation_by md. mohidul hasan manik_2014

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Genetics and Crop Improvement Participatory Varietals Selection of CIP germplasm with Mother & Baby Trial Approaches in Bangladesh 15 June 2014

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Genetics and Crop Improvement

Participatory Varietals Selection of CIP germplasm with Mother & Baby Trial

Approaches in Bangladesh

15 J

une

2014

1.To gather the opinion of the farmers (men and women both) consumers and technicians in the selection of possible new promising clones as varieties.

2. To characterize the preferences that exist in the different regions.

3.To validate a semi-structured methodology with the M&B design in order to be able to systematically compare results between sites and different years.

Mothertrial●

Baby 1 Baby 2

Baby 3

● ●● ● ●●

●●

Mother & Baby Trial Design

Mother Trial

Baby Trial

Experimental Details

Location Jessore Barisal Patuakhali

 

Year 2013-‘14 2013-‘14 2013-‘14Design RCBD RCBD  RCBDReplication 3 3 3 Spacing (cm) 60 X 25 60 X 25 60 X 25 Gross Plot Size

(m2)

4.50 4.50 4.50 

Date of Planting 29.11.2013 26.11.2013 01.12.2013 Date of

Harvesting

02.03.2014 04.03.2014 07.03.2014 

.1. Vegetative development:

• Identification selection criteria• Ranking selection criteria

2. Harvest:

• Evaluation tuber yield• Identification selection criteria• Ranking selection criteria • Organoleptic evaluation

3. Post-harvest:

• Sprouting & weight loss• Identification selection criteria• Ranking selection criteria

Main exercises / measurements during the selection cycle

VegetativeVegetative

Stage of Evaluation

HarvestHarvest Post-HarvestPost-Harvest

Main exercises / measurements during the selection cycle

Evaluation at vegetative stage

Rank of

order

Gathering and ranking of Criteria at vegetative stage

 

I

Jessore Barisal Patuakhali

a. Plant may be vigor

b. Diseases and pest can be less

Strong stem Virus resistant

II Plant short duration

Short Plant Strong stem

III Maximum canopy of plant

Dark green color leaves

Bacterial wilt resistant

Rank of

order

Reasons of selection

Jessore Barisal PatuakhaliMother Baby Mother Baby Mother Baby

I a. Plant may be vigorb. Less diseases and pest c. Strong stemd. Virus resistant

CIP-111 CIP-14 CIP-139 CIP-139 CIP-139  CIP-139

II a. Short duration variety b. Short plant sizec. Strong stem

CIP-14 CIP-127 CIP-127 CIP-126 CIP-127  CIP-112

III a. Dense canopy b. Dark green leavesc. Bacterial wilt resistant

CIP-126 CIP-130 CIP-14 CIP-112 CIP-130  CIP-111

Ranking of the Preferred Clones by Plot” (vegetative stage)

Evaluation at Harvest stageRank of order

Gathering and Ranking of Criteria” (time of harvest)

Jessore Barisal PatuakhaliI Disease and pest/free

resistant variety Good taste Low water contained

II Resistant to tuber moth Red skin color tuber Oblong shape tuberIII Good yield White skin color tuber Good taste

Ranking of the Best Clones by participants (time of harvest)

Rank of

order

Reasons of selection Jessore Barisal PatuakhaliMother Baby Mother Baby Mother Baby

I a. Disease and pest/free resistant variety b. Good taste

CIP-139 CIP-139 CIP-139 CIP-139 CIP-126 CIP-139

II a. Storage capacity of potato is excellent b. Red skin color tuber c. Oblong shape tuber 

CIP-126 CIP-115 CIP-115 CIP-127 CIP-139 CIP-126

III a. Good yieldb. Good tastec. White skin tuber

CIP-14 CIP-126 CIP-127 CIP-14 CIP-127 CIP-127

Yield performance

Average yield data of all three locations

Clone/VarietiesJessore yield t/ha Barisal yield t/ha Patuakhali yield t/ha Average

(t/ha)Mother

BabyMother

BabyMother

BabyCIP-111 (2nd) 46.07 33.99 31.85 22.74 42.05 28.96 34.28 (2nd )CIP-112 40.96 41.33 26.29 20.74 32.17 35.04 32.76CIP-115 35.92 35.92 25.18 21.11 16.43 29.03 27.27CIP-126 (3rd) 47.11 43.48 33.99 21.7 28.4 29.90 34.10 (3rd )CIP-127 38.15 28.37 27.77 22.07 29.03 28.92 29.05CIP-130 47.11 29.85 30.00 21.55 34.32 21.33 30.69CIP-139 (1st) 48.22 37.4 29.25 23.55 38.49 36.34 35.54 (1st)CIP-14 39.41 25.48 30.14 22.22 23.27 18.9 26.57LB-12 33.03 22.07 25.18 19.92 23.31 18.3 23.64DIAMANT 39.41 31.18 27.78 23.48 23 19.25 27.35ASTERIX 23.26 16.96 27.78 20.51 21.21 19.94 21.61CV (%) 16.15 12.63 6.71 8.00 3.58 2.92 -LSD (0.05) 3.34 6.61 3.20 2.90 1.69 1.26 -

Organoleptic Evaluation by participantsAverage organoleptic evaluation at all locations during harvesting period:

Clone/varieties

Jessore Barisal Patuakhali Avg.

App. Taste Tex. App. Taste Tex. App. Taste Tex.  

CIP-111 4.20 3.00 5.35 4.20 2.80 4.70 4.20 3.20 6.00 4.18

CIP-112 3.55 2.50 4.60 4.00 2.50 3.95 3.10 2.50 5.25 3.55

CIP 115 3.55 3.20 5.58 3.80 3.40 5.15 3.30 3.00 6.00 4.11

CIP-126 3.60 3.25 5.08 4.20 3.50 5.05 3.00 3.00 5.10 3.98

CIP 127 (3rd ) 4.25 3.00 5.90 4.50 2.60 4.45 4.00 3.40 7.35 4.38

CIP 130 3.45 2.30 4.68 3.80 1.90 3.95 3.10 2.70 5.40 3.48

CIP 139 3.15 3.20 5.28 3.30 2.50 3.95 3.00 3.90 6.60 3.88

CIP-14 3.20 3.35 4.90 3.30 2.90 4.25 3.10 3.80 5.55 3.82

LB-12 (1st) 4.15 3.60 6.10 4.20 4.20 5.45 4.10 3.00 6.75 4.62

Diamant 3.60 3.30 5.38 3.80 3.20 4.70 3.40 3.40 6.05 4.09

Asterix (2nd ) 4.30 3.40 5.93 4.60 3.50 5.10 4.20 3.20 6.00 4.47

Post-Harvest evaluation stage

Rank of order

Gathering and Ranking of Criteria (time of post-harvest)

  Jessore Barisal Patuakhali

I Disease and pest resistant variety

High storage ability under house hold

Less rottage loss

II Smooth potato skin

Less rottage loss

Late spurting

III Quick time require for boiling after storage

Less disease infection

Weight should not be decrease

Ranking of the Best Clones in Storage (post-harvest) at farmer’s house, at three locations.

Clones

Jessore Barisal Patuakhali

Global score

Order of Importance

Score: Men(N=9 corn)

Score: Women

(N=6 beans )

Score: Men

(N=10 corn)

Score: Women

(N=5 beans )

Score: Men

(N=10 corn)

Score: Women

(N=5 beans )

CIP 111 5 4 11 2 0 0 22 VICIP 112 11 7 2 0 0 0 20 VIICIP 115 0 0 2 3 0 1 6 XCIP 126 0 0 0 0 12 2 14 IXCIP127 (1st) 8 2 7 8 19 11 55 ICIP 130 0 0 0 3 0 0 3 XICIP 139 (3rd) 0 0 26 10 0 0 36 IIICIP 14 18 15 0 1 0 0 34 IVLB 12 10 7 9 0 0 0 26 VDiamant (CV) 2 1 0 3 8 2 16 VIIIAsterix (CV) (2nd ) 0 0 3 0 21 14 38 IITotal 54 36 60 30 60 30 270 -

“Standard Evaluation: Number of Sprouts, Tuber Weight and Health” (post-harvest)

Clone/VarietyWeight loss (%) No. of rotted No. of Sprout/tuber

Jsr. Bsl. Pkh. Avg. Jsr. Bsl. Pkh. Avg. Jsr. Bsl. Pkh. Avg.

CIP 111 20.85 19.17 18.23 19.42 1 3 1 1.67 5 4 6 5.00

CIP 112 20.00 17.77 18.33 18.70 0 2 2 1.33 1 1 0 0.67

CIP 115 18.40 19.56 19.83 19.26 0 4 1 1.67 10 6 6 7.33

CIP 126 11.02 13.32 11.72 12.02 0 3 3 2.00 5 5 5 5.00

CIP127 13.93 12.63 11.87 12.81 0 1 4 1.67 10 9 6 8.33

CIP 130 12.73 17.26 14.83 14.94 0 3 1 1.33 10 7 4 7.00

CIP 139 12.00 16.76 13.74 14.17 0 2 7 3.00 2 2 0 1.33

CIP 14 10.92 14.52 12.50 12.65 0 2 2 1.33 10 8 4 7.33

LB 12 10.17 9.78 16.34 12.10 0 2 6 2.67 4 5 5 4.67

Diamant (CV) 16.96 18.70 18.72 18.13 0 2 3 1.67 7 8 5 6.67

Asterix (CV) 16.36 17.17 20.68 18.07 1 3 3 2.33 4 6 1 3.67

Considering farmers choice on vegetative stage evaluation, organoleptic test, yield performance and storability evaluation, there were five CIP clones namely were selected for advanced yield trial (AYT) in next year.

CIP 139 (396311.1)CIP 126 (392797.22)CIP 127 (392820.1)CIP-112(380606.6) and CIP-111 (380583.8)

Conclusion