cm2859 cryostat microtome

CM2850 Cryostat Microtome

Operation manual

ForewordCM2850 Cryostat Microtome is a

semi- automatic microtome whose design

concept comes from customers all over the

world , it can meet different requirements for

tissue section.

To promise the instrument to work

properly , safely and perform durable , it is

necessary to read the operation manual

carefully.

Our company are not only responsible

for the repair , but also train distributors'

ability of repairing . To repair the product

smoothly in time , please contact your local

distributor. Notice: With the development of

technology and constantly updating of

products this manual will make the

corresponding changes . Please forgive us not

to notify the improvement of technical data

and structure.

1", Safety Notes

1. 1Overview

The Operator Manual contains important safety instructions and information . In orderto operate the instrument safely , the operator must read carefully prior to startup theinstrument. It is very important to ensure safety and avoid instrument damage . Please keepthe manual close to the instrument to read easily at any time.

This instrument was built and test in accordance with the safety regulation as specifiedbelow:GB4793.1-2007 Medical Electrical Equipment First Part:Current Requirements for safety

1.2 Safety Warning

Every principle person should read carefully and carry out strictly according to thefollowing safety notes relating to transport , installation ,calibration , operation ,maintenance, cleaning and all aspects of the instrument.1.2.1 Warning during transport and installation

1.2.2 Warning of Operation

CM 2850 Cryostat Microtome ---1--- /' 201211190001EN

2", Performance & Parameters

2.10verview- Instrument Components

Waste Container

Control Panel

Quick Freeze Shelf

Heating Glass

Handwheel

Specimen Clamp

Lock Lever

Blade Holder

Tool Shelf

Heat

Peltier on freezeshelf freezingArea

Display Control Panel

StationaryExtractor

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in 0.5 J-lmincrementsin 1J-lmincrementsin 2J-lmincrementsin 5J-lmincrements

8 Electric coarse feed: SlowRapid

8 Section thickness range:

300 +30J-lmls900J-lmls+30J-lmls

o to 100J-lmo to 3J-lm,3 to 10J-lm,10 to 20J-lm,20 to 100J-lm,

8 Trimming thickness range: 10 to 600J-lm10 to 50J-lm,50 to 100J-lm,100 to 600 J-lm,

in 5J-lmincrementsin 10J-lmincrements

in 50J-lmincrements

8 Retraction: O~SOJ-lm,in 5J-lmincrements

8 Specimen feed: 15mm ±0.2mm

8 Vertical stroke: 4Smm±lmm

8 Specimen Max: 35x35mm

8 Repositioning of blade holder base(left-right) 50±lmm

8 Dimension: Length: S05mm,Width: 7l5mm,Height: 1210mm,

8 Weight: About l30kgs


3.3 Installation

1

4

56

8

9

10

3.3.1 UnpackingAs showed in the picture, the

following is the instrumentaccessones:

<::> Carton Cover (1)<::> Upholder (2)<::> Comer Supportl (:3)<::> Carton body (4)<::> Glass Support (5)<::> Comer Support 2 (6)<::> Comer Support 3 (7)<::> Handwheel (8)<::> Blade Holder (9)<::> Carton Base (1 a )<::> Cryostat Microtome ( 11 )<::> Comer Support 4 (2)

Remove the cover (1) and cartonbody (4) , then take the following

things in order: Upholder (2) , ComerSupport 1 (3) , Comer Support 2(6) ,Comer Support 3(7) , Comer Support4 (12) , Handwheel (8) , Blade Holder(9), Cryostat Microtome (11) , GlassSupport (5) . Then take out otheraccessories in the inner box package toinstall the instrument .

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3.4 Electrical Connection

• Insert the fuse(23) into the fuse socket(22)as it showed in the Fig and insert thewhole fuse socket into the socket(2l) .

• As Fig shows power switch . Turn off asthe left fig and tum on as right fig .

• After turning on the power , display panellight and the specimen block will be back tothe original base position automatically with abeep.


" + ", "-" The two keys is used for settingsection/trimming thickness , time and temperatureparameters.Section thickness value: 0 to 100flm

o to 3flm, in 0.5flm increments3 to 1Oflm, in 1flm increments10 to 20Jlm, in 2Jlm increments20 to 100flm, in 5Jlm increments

Trimming thickness value: 10 to 600Jlm

10 to 50flm, in 5Jlm increments50 to 100flm, inlOflm increments100 to 600Jlm, in 50flm increments

is setting key .This key is used to active setting of the section/trimming

thickness, retraction ,temperature and time.

Indicator light on Section modeLOCK: if its led lit up , it shows the

hand wheel is locked, and it is disabled at thismoment to slice section.

~ , if its led lit up , it shows it is defrosting now.

SECT/TRIM is the alternative select key betweensection mode and trimming mode.

RETRA is retraction key.This key is used to enter in retraction function.

1 is temperature setting key.

It is a cycle key to select desired item amongspecimen clamp temperature cryo chambertemperature, freezing shelf temperature, and ambienttemperature .


4.1.2 Left control panel

The buttons is tocontrol the specimenforward or backwardmoving , it has twospeed.

by pushing it again.

ZZ is the button to turn on/off sleep function.

/' Keyboard locking button

This key is used to lock the keyboard.

Hold on this button until the icon "

displayed on display panel ( As showed in left

Fig ), the keyboard has be locked. All other keys

are disabled at this moment, and release the lock

by long press the button.

Fast forward and fast backward key.Adjust the specimen position rapidly , the speedcould reach to 900 +30llm/S.

Slow forward and fast backward key.Adjust the specimen position slowly, the speedcould reach to 300 +30llm/S.

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Installation of blade holder and baseAs showed in picture, slide the blade holder(l3)

along the track(14) in cryostat microtome, rotate thehandle of lever 2(8) to horizon position to lock theblade holder.

15

Tighten the blade and holder and the base holder with thefour fixed poles and adjust the rotating angle as requirementbefore tighten it .The rotating angle is from 0 to 10 degree ( see the picture) ,the user can adjust the angle in this range according to therequirement.

Anti roll plate adjustmentAnti roll plate is rectangle organic glass , do asfollowed:8 Insert the blade , and rotate the fixed pole(7) to

15 vertical positon for tighten it8Lock the two anti roll plate knobs(ll).8Adjust the bolts (15) with 3 mm allen key to makeblade be parallel with anti roll plate , then tightenthe blots(l5).8 Turn the knob(l2) to adjust the hight of anti rollplate.

.•. Attention:

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4.4 Temperature setting

4.4.1 Specimen clamp temperature setting

Board area

I + -Press! shortly and the line of temperature board area gets

TTl thicker. When the display shows "@)thatit have entered into6 U 20U 08t 08: 08 specimen clamp temperature display status.

ls c::::J 1 N=2221im 170023+ 5 sJ-Then press 0 to enter into temperature setting situation.

-The value can be adjusted by_ as showed in left Fig.

-After setting up, press 0 again to exit or let it alone for 5seconds which will exit automatically.

The temperature range is between -50 and 50° C.

r

! Specimen clamp temperature

4.4.2 Cryo chamber temperature setting

Cryo chamber temperature

-Press! shortly and the line of temperature board area gets

thicker.

When the display shows ij] that it have entered into cryo

chamber temperature display status.-Then press 0 to enter into temperature setting situation . The

value can be adjusted by_ as showed in left Fig.

-After setting up , press 0 again to exit or let it alone for 5seconds which will exit automatically.The temperature range is between -50 and 50° C

4.4.3 Freezin shelf temperatureBoard area

r TTl6 U 20U. 08t 08:08lsc::J1NIIl2U1· 1.~"5SJ

Press! shortly and the line of temperature board area gets

thicker. When the display shows [I that it have entered into

freezing shelf temperature situation . The time on the displayis actual temperature of freezing shelf.

Freezing shelf temperature

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4.5.2 Defrosting time setting

"Press '-/ and the line of time board area gets

thicker. When the light III is on which means to the item

defrosting time have been selected (as in left Fig) , thenpress Oto enter into defrosting time setting . The value can

be setted through_; Press 0 to set minute after hour

setting , then press 0 again to set week . After finishing the

defrosting setting , press 0 to enter into disinfection time

setting or let it alone for 5 seconds to exit automatically.

4.5.3 Disinfection time

Board area


thicker .When the light _ is on which means to theitem disinfection time have been selected (as in left Fig) ,then press 0 to enter into disinfection time setting . The

value can be setted through_; Press 0 to set minute

after hour setting , then press 0 again to set week . After

finishing the disinfection time setting , press 0 to enter

into sleep time setting or let it alone for 5 seconds to exitautomatically . When the disinfection is on , "03" icon(shown at left) will displayed above the time zone. And thedisinfection function is activated means 03 disinfection andUV disinfection both are activated together.

4.5.4 Sleep time setting


.. "tIme settmg , press '-" to enter into wakeup time setting or let it

is on which means to the itemthicker .When the light

sleep time have been selected (as in left Fig) , then presso to enter into sleep time setting . The value can be setted

through_; Press 0 to set minute after hour setting ,

then press 0 again to set week . After finishing the sleepBoard area


from specimen , press to move the specimen

clamp forward or backward quickly; When the blade is close

8 FeedPress the key to adjust the specimen position to be parallelwith the blade edge for section . When the blade is far away

to adjust the position slowly.to the blade, press

8 SectionJust rotate right handwheel to section/trim ,and it is thecounter of slicing number.

On trimming situation, it shows 0 and could starts to countwhen it turns to section situation.

4.7 Retraction settingRetraction

r T TTl20 U 20U 08t 08:08trc:::J 1N"'2U 1.• 1ltD23USJ

Press RETRA one time first and the line of retractionboard area gets thicker, then press 0 to enter into

retraction setting. The value can be setted by _ .

After finishing the setting , press 0 again to exit or let italone for 5 seconds to exit automatically.

4.8 Section Reference

1~ When using cryostat microtome, please note that proper sectioning speed and correct anti-roll

plate adjustment are important factors to obtain good quality section. The proper section speed

is acquired through practical experience. The positions of anti roll plate adjustment is various,

some of them are mutual influence, so they need to be adjusted carefully. The anti roll plate

could not put on the cutting edge .

2 ~ When the tissue is freezing , the water in the tissue is frozen , the tissue get harder . The

hardness changes with temperature, the lower the temperature is , the harder the tissue will be .

It needs to be worked through in practice to get better quality section under which temperature .

eM 2850 Cryostat Microtome "' __ -_--2_1-_-- __ / 201211190001EN

5, Clearance & Maintenance

5.1 Clearance

8 Cleaning the instrumentUse the wet cloth to clean the areas always be touched during operation, for example the

handle and the base holder locking lever and the storage area on the crust. Use the dry cloth toclean other appearance.

8 Cleaning the clamp

Specimen clampPosition (1) which is often touched when operation, andposition (2) which is always contacted with thespecimens . These two positions, especially position(2)are very easy to be polluted , so need to be cleanedfrequently to ensure ordinary operation.

8 Cleaning the blade holder

\

Take down all the parts of blade holder as it isshowed in the picture ad then clean all the partsseparately , especially the following easily pollutedparts : the sliding guide , fixed pole , blade clampand the joint of the parts . And remember to cleanthe blade clamp every time before installing theblade to ensure good section.

To obtain a high quality section, it is important to keep cleaning the instrument. Sothe user must clean the instrument periodically or irregularly according to the totalsectioning quantity to obtain the best section .

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6... Trouble ShootingBelow you find a list of the problems that most frequently occur. And they are most causedby the operator, sp please read the operation manual carefully before using it .

Problem

• No display, noreaction to buttonspressed after theinstrument is switchedon

• The section isuneven from the secondsection

• Section curl

• Section soften

• The section sticks tothe blade or anti rollplate

• Section splinter

Possible causes

• Mains cable not properlyconnected or it is break in thecircuit

• Mains fuses defective

• The input voltage is notmatched with the one showed inthe marks

• The section angle of the bladeis too small .

• The clamping setting is notsteady

• The space between anti rollplate and blade is too small oranti roll plate is lower than theblade

• The temperature of blade oranti roll plate is too high

• The blade or anti roll plate ispolluted

• Temperature too low for tissuecut

Corrective action

• Reconnected the main cable orreplace it .

• Replace the fuse.

• If it is not matched, please callfor the professional personnel .

·Alternately thick and thin sectionare produced . In extreme caseevery second section is skipped,being followed by a verythickness . Systematically trywider clearance angle setting untiloptimum angel width has beenfound.

• Check if all the screw aretightened.

• Adjust the anti roll plate.

• Extend the cooling time of bladeor anti roll plate.

• Clean the blade or anti roll plate

• Reduce the cooling time andadjust cryo chamber temperature


Standard accessories list

CM2850

No Accessory Name Qty Notes

1 Cryostat Microtome 1 unit

2 Blade Holder 1 set

3 Handling wheel 1 pc

4 Specimen Clamp 10 pcs

5 M5 Allen Wrench 1 pc

6 M3Allen Wrench 1 pc

7 7mm Wrench 1 pc

8 Disposable blade 1 box

9 Power cord 1 pc

10 Brush 1 pc

11 Fuse 2 pcs 220V/8AllOVI15A

12 Operation Manual 1 pc

Problem

• The surface of thesection is in wave.

•The section flies awayand sticks to themicrotome or otherobjects near themicrotome

• The blade producessounds when section,and the section are

scratched and showvibrated mark

Possible causes

·The gradient of the blade is notproper.

• It is effected by static.

·The gradient of the blade is notproper.

Corrective action

• Readjust the gradient.

• Increase the surroundinghumidity 0 get rid of the static .

• Reinstall the blade and adust thegradient of the blade.

If there any other troubles can not be resolved, please contact with the manufacturer.

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5.2 Maintenance

• Put the fuse (1) in the installation part (2)and then insert the installation part into thesocket (3) .

CM 2850 Cryostat Microtome ...•••••• ---24--- ./.....•..._--_/ 201211190001 EN

The sectioning temperature range of most fat-free without formalin fixed is best between -13 °C

and _23°C .

3~ To obtain high quality section, please pay attention to the following aspects:

( 1) Proper cryo chamber temperature selection.

( 2) Correct section operation.

(3) careful anti-roll plate adjustment.

( 4) Sharp blade and appropriate sectioning angle

( 5) If the blade is clamped tightly.

( 6) If the specimen is clamped tightly.

Select a proper angle of the sectioning and specimen. The smaller the angle, the lower the section

compressed. The harder the specimen, the larger the angle . If the section is still not good, please try to

increase the angel from 00•

Now there is no general rule to make sure proper of different specimen, so try to find a appropriate

angle of different specimen for good section.

4~ The frozen section is in contrary with paraffin section. It doesn't section long side of tissue but short

side in frozen section. Try to make contact line of tissue and blade shorter.

CM 2850 Cryostat Microtome "'" __ -_--2_2-_-- __ / 201211190001EN

alone for 5 seconds to exit automatically. When the sleepfunction is activated , "Zz" icon (shown at left) willdisplayed above the time zone. The sleep time can be settedseparately from Monday to Sunday.

4.5.5 Wake up time setting

Press and the line of time board area gets thicker .

When the light is on which means to the item wakeup

time have been selected (as in left Fig) , then press 0 toenter into wakeup time setting . The value can be setted

through_: Press 0 to set minute after hour setting, then

press 0 again to set week. The wakeup time can be settedseparately from Monday to Sunday. If the user does not needto use this machine on a certain day , please set the hour valueas 00 and the minute value as 60 ( 60 is ineffective time whichcan not wake up automatically) . After finishing the sleep time

setting , press G to exit or let it alone for 5 seconds to exit

automatically.

4.6 Section/Trimming setting

Section thickness 8 Section setting

Press SECT /TRIM shortly and the line of section board areagets thicker. When the Letter before progress bar becomes "s"which means it has enter into section situation, then press 0 toenter into section setting . The section value can be setted by

_ . After finishing the setting, pressO again to exit or let

it alone for 5 seconds to exit automatically.

Board area

T T T 1U 20U 08t ..08: 08~rHom1 -e 1181,m~

Progress bar

Trimming thickness

r T T T 120U 20U ost 08:08~r-..1.•1·...·J

Board area

8 Trimming settingPress SECT /TRIM shortly and the line of trimming board area

gets thicker .When the Letter before progress bar becomes "T"which means it has enter into trimming situation, then press 0to enter into trimming setting. The section value can be setted by

_ . After finishing the setting, press 0 again to exit or let

it alone for 5 seconds to exit automatically.

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4.4.4 Ambient temperatureI I

r T tTl6 U 20U oat 08: 08lst=:J 1 N=2221 111l23t5eJ

Ambient Temperature

Press lshortly and the line of temperature board area gets thicker.

When the display shows "t that it have entered into ambient

temperature situation . The time on the display is actual ambienttemperature .

4.4.5 Enable specimen clamp peltier function

r .TT* T'6 ,U .. 20U ..-. 0.,·· •.8.\ ..0.8. :081N-2

221a1.t·"'·J

4.5 Time setting

4.5.1 Real time setting

Hold on ··.l .. for a few seconds to tum on/off specimen clamp

peltier function . When the function is activated , the above oftemperature area will display icon "*" as in left Fig, itsworking time is 15 minutes; the specimen clamp peltierfunction is able to be activated when the temperature of cryochamber is below 80 c.

Press 0 shortly and the line of time board area gets

thicker .When the light BIll is on which means to the item real

time have been selected (as in left Fig) , then press Oto enter

into real time setting . The value can be setted through_;

Press 0 to set minute after hour setting , then press again/".

to set week . After finishing the real time setting , press ~ to

enter into defrosting time setting or let it alone for 5 seconds toexit automatically.

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4.3 Clamping the specimen and Inserting the blade

3

• •

1 Clamping the specimen

As showed in the picture , rotate the clamplever (2) , and insert the specimen(3) into clamp(1) ,then loosen the clamp lever (2) .

Blade insertingAs showed in the Fig , loosen the fixed lever(5) and push the blade(6) into blade holder(4) ,then rotate the lever(5) to clamp the blade.It uses blade in the picture , blade can be

5 divided into two types: low & high profile.If the high profile blade is needed , twist thefour screws (7)out with 3mm Allen key, thentake out anti roll plate module (8) , twist outthe two screws (9) on base plate (10) to takeout the base plate (10). After inserting theblade, and refixed the anti-roll plate module(8)with the four bolts (7) .

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4.2 Installation of holder

93

\

L

6 11

5

1

As fig shows , the blade holder be composed of the following parts : blade holder base (1) ,longitudinal movable jaw (2) , rotating movable jaw (3) , movable knife clamp(4), fixed knifeclamp (5), anti-roll plate (6) , fixed lever 1 (7) , fixed lever 2 (8) , fixed lever 3 (9) , fixed lever4(10) , anti-roll plate lock knob (11) , anti-roll plate height adjusting knob(12) .

8 Insert the longitudinal movable jaw (2) into the track of blade holder base(1) , and rotatelever 2(8) to lock firmly. Then adjust the handle of lever 2(8) to horizon position.

8 Adjust the rotating movable jaw(3) into longitudinal movable jaw (2) , and rotate thelever 4(10) to lock firmly.

8 Put movable knife clamp (4) and fixed knife clamp(5) on rotating movable jaw (3) ,rotate the lever 3 (9) to lock it firmly. Adjust the handle of lever 3 (9) to vertical position.

8 Rotate the lever 1 (7) to lock the movable knife clamp (4) and fixed knife clamp (5) .


() is a cycle key to select desired time mode.

Short push the button to select: real time, setting of

real time, defrosting time, disinfection time , sleep

time and wake up time.

C3 is the real time indicator light.

The real time setting is ready when real time light is on

~ is defrosting time indicator light.

The defrosting time setting is ready when the

defrosting time light is on.

03 is disinfection time indicator light.

The disinfection time setting is ready when the

disinfection time light is on .

ZZ is the sleep time indicator light.

The sleep time setting is ready when the sleep time

light is on.

WAKE UP is the wake up time indicator light.

The wake up time setting when the wake up time

light is on .

~ is the key to start defrosting immediately.

Hold on this button for about 5 seconds to start

defrosting immediately, Meanwhile the

defrosting indicator light is on. The process lasts about

30 minutes, and go back to work status automatically,

light off.

UV is the button to tum onioffUV disinfection.

Short push this button to tum on UV disinfection ,

and tum it off by pushing it again.

03 is the button to turn on/off 03 disinfection

immediately

Short push this button to tum on 03 disinfection and

tum it off by pushing it again.

a is the button to turn on/off lamp.

Short push this button to tum on lamp and tum it off

CM 2850 Cryostat Microtome "'" __ -_--1_2-_-- __ / 201211190001EN

4~ Operation

4.1 Control panel function & Control

All the parameters are set and displayed via control panel . After power on , use thecontrol panel to operate the instrument as followed:

4.1.1 Display Panel

Display area show main informationsuch as : section thickness , trimmingthickness, retraction , cryo chambertemperature specimen clamptemperature and time.

The area is to set the value ofsection thickness , trimmingthickness,retraction ,temperature and time.

0: Setting Key

Indicator light

LOCK : Locking lightfor handle wheel.

~ : Defrosting light

SECT/TRIM: AlternativekeyRETRA: Retraction key

1. : Cycle select key among

specimen clamp temperature ,cryo chamber temperature ,freezing shelf temperature andambient temperature

: Cycle key to select

desired time mode

Q) :Real time

~ :Defrosting time

03: Disinfection timeZz: Sleep timeWAKE UP: Wake uptime

~ : Immediate defrosting

keyUV: UV disinfection key03: Ozone disinfection key

, I •

-Q-: Lamp key

ZZ : Immediate sleep key

: Key-board

lock key.

Three lines in blue-mask LCD.The first line displays current status ( Key lock, defrosting,sleep and disinfection icons in order)The second line displays section thickness, retraction, cryochamber temperature and time etc.The third line displays section/trimming situation,specimen clamp progress , slice quantity , temperaturesituation and week time

CM 2850 Cryostat Microtome , __ --_-1o_---__ / 201211190001EN

3.3.2 Handwheel assembly

• Take out the handwheel accessoriesfrom handwheel box (8) .• Tighten the handwheellever (14)

into handwheel (13) with 7mmwrench.• Insert bearing(l5) , hand wheel

cover (16) ,bearing (15) andhandwheel washer (17) in order.• Tighten them with M5 X 16

hexagon socket screw .

3.3.3 Blade holderTake out the Blade holder (19)

from the box, push it along the track as itis showed in the Fig , and then rotate theBlade holder lock lever(20) to lock theblade.

CM 2850 Cryostat Microtome ---8--- / 201211190001 EN

3" Preparation before operating

3.1 Installation site requirement

8 The floor stand instrument fixed 4 wheels and 2 fixed support feet . The front only beused during movement. Remove the package and move the instrument to the installation site,then adjust the two fixed support feet and make the front wheels disabled.8 Start the instrument after let it rest for at least 2 hours.8 Ensure no other goods around the instrument. At least 300mm distance must be kept forventilation and heat dissipation.8 Ensure the working temperature and humidity must be accord with the specification inmanual.8 Ensure leaving enough running space for rotating the handwheel .

..•. Notice:

3.2 Standard Delivery

8 Cryostat Microtome8 Blade Holder8 Handwheel8 Specimen Clamp8 M5 Allen Key8 M3 Allen Key8 7mm Wrench0) Disposable Blade0) Power Cord0) Fuse0) Brush0) Operate Manual

1unit1unit1pc10 pcs1 pc1 pc1pc1 box1pc2 pcs1pc1pc


2.2Performance Index

CM2850 is a semi-automatic cryostat microtome whose specimen forward andbackward movement are controlled by motor . So it can get high quality section and easilyoperated. The following is its performance :

o The whole instrument followed human engineering theory as well as artisticappearance, made by numerical control machine.

o The retraction function can prevent the specimen touch the blade sharp during lift.o It equips counter which is able to show total quantity of slice.o It equips calculator function to display the total thickness.o Artificial intelligence interface that easy to learn and operate.o Adopt UV and 03 to sterilize for 30 minutes every time .o The specimen clamp semiconductor refrigerating function is enable or disable.o Defrosting at fixed time or manuallyo Large freezing shelf could load 8 cassettes simultaneously.

2.3Technical Parameter

o Environment requirements: Temperature Range: + 15DC_+ 30 DC,Air Humidity: :::;60%Working pressure: (86------106)kPa:

o Nominal Voltage: AC 220V/AC 110V

o Normal Frequency: 60 Hz

o Power: :::::;1000VA

o Fuse: 8A/15A

o Safe Classify: Classify I - Type B

o Lowest freeze chamber Temperature : -35 +2 DC

o Lowest freeze shelf Temperature: -45 +5DC

o Lowest temperature of peltier unit on freeze shelf: -55 +5DC

o Lowest temperature of peltier unit on specimen clamp: -50 + 5 DC(Working time ofpeltier: 15 minutes)

CM 2850 Cryostat Microtome , __ -_--4_--- __ / 201211190001 EN

1.2.3 Warning during Clearance and Maintenance

1.3 Safety Device

Handwheellocking mechanismAs showed in Fig, it is the lock lever .

Clockwise push the lever( I) until it lock thehandwheel at the upright position . To unlock thehandwheel , rotate counterclockwise the lever(l) .

1 As showed in Fig, the handwheel is locked.If the lock activated , the "Lock" light will

light on display panel , while the instrument can'trun and the AUTO key is invalid.

instrument.

CM 2850 Cryostat Microtome , __ --_-2_--- __ / 201211190001EN

Contents

1"\ Safety Notes 11.1 Overview 1,."." ••••• 1,.".,1 •••••• 11."."." •• 1,.".,1.,1.,1 II' ••• 1,." ••••• 1,." ••••••••••••••••• 11.2 Safety"Warning. II 1,."."."." •• 1,."."." ••• 1,." •• II 11."."." ••••• II •••• II •••••••••••••••••• 11.3 Safety"Device. II •••• 1,.".".,1.,1 1,.,1 •• II 1,." •••• 1,."." •• I" ••••••••••••••••••••••••••••••••••••••••• 2

2" Performance & Parameters 32.1 Overview of Instrument Component 32.2 Performance Index 42.3 Technical Parameters 4

3" Preparation before operating 63.1 Requirement of Installation .. ,. 1,.".".,1 1,." •••• I" •••••••••• 1,." •••••••••••••••••••••••••• 63.2 Standard Delivery 63.3 Installation 73.4 Electrical Connection 9

4, Operation 104.1 Operate Panel Function &Control. 104.2 Installation of Blade Holder 144.3Clamping The Specimen&Inserting The Blade 164.4 Temperature Setting 174.5 Time Setting 184.6 Section Setting 204.7 Retraction Setting 204.8 Section Reference 21

5, Clearance & Maintenance 225.1 Clearance 235.2 Maintenance 24

6, Trouble Shooting 24

HESTION

1 :::~~:7[: :~:~ ::~J

Presented in this Operation manual are the structure, functions and

using notice . Reading carefully prior to operating the instrument.

cm2859 cryostat microtome

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