control sirna
DESCRIPTION
A. B. c-MYC siRNA. c-MYC siRNA. c-MYC siRNA. Control siRNA. Control siRNA. Control siRNA. c-MYC siRNA. Control siRNA. 250. 200. 150. p21 protein expression (% control). 100. 50. 0. p21 (21 kDa). β -Actin (42 kDa). D341. D425. DAOY wt. DAOY M2. DAOY V11. DAOY wt. 1. 0.8. - PowerPoint PPT PresentationTRANSCRIPT
A B
DC
Control siRNA
c-MYC siRNA
0
20
40
60
80
100
120
DA
OY
wt
DA
OY
V11
DA
OY
M2
D34
1
D42
5
Clo
noge
nic
surv
ival
(%
con
trol
)
*
*** ***
* *
Control siRNA
c-MYC siRNA
DAOY wt
DAOY V11 D341
0
20
40
60
80
G1 S G2/M
0
20
40
60
80
G1 S G2/M
DAOY M2
0
20
40
60
80
G1 S G2/M
0
20
40
60
80
G1 S G2/M
D425
0
20
40
60
80
G1 S G2/M
**
*
***
**
*
*
Tot
al C
ell c
ount
in %
Tot
al C
ell c
ount
in %
Tot
al C
ell c
ount
in %
Tot
al C
ell c
ount
in %
Tot
al C
ell c
ount
in %
DA
OY
wt
DA
OY
V11
DA
OY
M2
D34
1
D42
5
β-Actin (42 kDa)
p21 (21 kDa)
0
50
100
150
200
250
p21
prot
ein
expr
essi
on (
% c
ontr
ol)
Control siRNA
c-MYC siRNA
Control siRNA
c-MYC siRNA
DAOY wt
DAOY V11 D341
DAOY M2D425
0
0.2
0.4
0.6
0.8
1
Day 0 Day 2 Day 4 Day 6
Pro
lifer
ativ
e ac
tivity
(O
D 4
90nm
)P
rolif
erat
ive
activ
ity
(OD
490
nm)
0
0.2
0.4
0.6
0.8
1
Day 0 Day 2 Day 4 Day 6
Pro
lifer
ativ
e ac
tivity
(O
D 4
90nm
)
0
0.2
0.4
0.6
0.8
1
Day 0 Day 2 Day 4 Day 6P
rolif
erat
ive
activ
ity
(OD
490
nm)
Pro
lifer
ativ
e ac
tivity
(O
D 4
90nm
)0
0.2
0.4
0.6
0.8
1
Day 0 Day 2 Day 4 Day 6
0
0.2
0.4
0.6
0.8
1
Day 0 Day 2 Day 4 Day 6