cr2014135x3
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CRTRANSCRIPT
10um50um 10um
2um
2um10um
10um
TSPAN4
10um
B
A
C
Supplementary information, Figure S3 CLEM analysis.
A. NRK cells stably expressing TSPAN4-GFP were cultured on Photo-Etched coverslip
pretreated with 10 μM FN. There are letters in Photo-Etched coverslip. For this
particular cell, is the letter AM (highlighted by white dotted line). The letter can be
obtained by bright field of confocal microscope and used to be positional information
of interested cells (left panel). Based on this positional information of interested cells,
this particular area is choose for ultra-sectioning during TEM sample preparation,
which usually contain 20~30 cells. Enlarged images are shown in the right panels. B
and C. The same cell observed by confocal microscope and TEM, the cell nucleus
highlighted by dotted lines. Enlarged images are shown in the right panel.