cs-ca-gfp map - riken...cs-ca-mcs 9679 bp mlui 228 noti 1521 psti 2573 ecori 2579 apai 3424 xbai...
TRANSCRIPT
CS-CA-MCS
9679 bp
MluI 228
NotI 1521
PstI 2573EcoRI 2579
ApaI 3424
XbaI 4188
(PstI) 4311(EcoRI) 4317(PstI) 4322BstXI 4332(NotI) 4343XhoI 4350
ApaI 5661
CMV RU5
5' SD
RRE
3' SAcPPT+CTS
CAG
MCSPREdel U3RU5
BGH pAf1 ori
SV40 pro & ori
Zeo
SV40 pA
ColE1 ori
Amp 1
Miyoshi
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Plasmid name: CS-CA-MCS Plasmid size: 9679 bp Note CMV: Human cytomegalovirus immediate early promoter y: Packaging signal 5’SD: 5’ splicing donor site 3’SA: 3’ splicing acceptor site RRE: Rev responsive element cPPT: Central polypurine tract CTS: Central termination sequence CAG: CMV immediate early enhancer and chicken β-actin gene promoter MCS: Multiple cloning site PRE: Woodchuck hepatitis virus posttranscriptional regulatory element del U3: Deletion of enhancer and promoter sequences in the U3 region BGH pA: Bovine growth hormone polyadenylation signal SV40 pro & ori: SV40 early promoter and origin Zeo: Zeocin resistance gene SV40 pA: SV40 polyadenylation signal Amp: Ampicillin resistance gene
Miyoshi
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y