developing breeding lines with anthracnose stalk rot resistance from exotic maize germplasm

College of Agriculture and Life Sciences

Department of Plant Breeding

Developing Breeding Lines with Anthracnose Stalk Rot Resistance

from Exotic Maize Germplasm

Margaret Smith



Anthracnose Stalk Rot

• Colletotrichum graminicola (Ces.) G.W. Wils.

• Increased lodging, reduced yield

• Best control through resistant varieties



Overall Project Objective

Develop temperate-adapted maize inbreds

with both anthracnose stalk rot resistance and

good yield potential

from GEM breeding populations



Selection and Breeding Scheme

Testcross yield evaluation of these 5 populations(1996)

Selected 4 best yielding 25% exotic : 75% temperate populations

Testcrosses of broader selection of S6 families(2002)

Used both public and Holden's testers

Repeat for S4 families(2000) and

S5 families (2001)

Selfing and selection in S3 families(1999)

Selfed and injected 8 plants/S3 family


Selfed and injected 8 plants/S2 family

Repeat for S4 x tester crosses(2000 - 3 locations, 2 reps/loc.)

and S5 x tester crosses(2001 - 2 locations, 2 reps/loc.)

Yield trials of S2 x tester crosses(1999)

3 locations, 2 reps/location

Testcrosses of S2 families(1998)

Crossed to SS or nSS public line cross testers


50 S1 families per populationSelfed and injected 8 plants/S1 family

Selfing within the 5 selected populations(1996)

Made about 100 selfs in 25% exotic : 75% temperate populations

ASR screening of GEM Populations(1995)

Selected 5 most resistant



Selected Populations

1) FS8B(T):N1802

2) CH04030:S0906

3) AR01150:N0406

4) GOQUEEN:N1603



Means by source population for anthracnose stalk rot ratings, 2001

Means of S5 Means of selectedfamily ratings (%) S5 plant ratings (%)

No. Mean No. MeanPopulation Families (± s.d.) Plants (± s.d.) FS8B(T):N1802 6 11.7 (± 1.8) 6 14.0 (± 5.3)CH04030:S0906 9 11.5 (± 1.6) 12 12.6 (± 7.5)AR01150:N0406 6 16.1 (± 4.2) 9 14.5 (± 7.4)GOQUEEN:N1603 9 16.0 (± 6.6) 13 10.6 (± 9.4)

Resis. check (01/31*) 48.0 LB31B 50.6Susc. check (B37) 73.2 * Our most resistant inbred at present, currently being released.



Yield Data - 2 locations, 2001Yield Moist. Y/M Lodging (%)

S2 Family Tester (bu/A) (%) Ratio Root Stalk

FS8B(T):N1802-490 B73/CD1 118.5 21.9 5.6 0 19FS8B(T):N1802-491 “ 122.8 21.7 5.8 1 16FS8B(T):N1802-492 “ 119.7 26.0 4.7 0 13FS8B(T):N1802-493 “ 123.5 25.0 5.0 0 16CH04030:S0906-476 RD6501/ 118.5 22.7 5.2 0 45CH04030:S0906-477 RD6502 120.1 21.7 5.6 0 31CH04030:S0906-478 “ 112.5 21.8 5.2 0 24AR01150:N0406-496 B73/CD1 112.9 20.8 5.5 0 43

Pioneer 34G81 166.1 20.0 8.4 8 7Agway 795 128.1 25.0 5.2 0 7Doeblers 596XY 125.3 21.1 6.0 0 15

Coefficient of variation (%) 13.3 5.5



Yield Data - 1 location, 2001Yield Moist. Y/M Lodging (%)

S2 Family Tester (bu/A) (%) Ratio Root Stalk

AR01150:N0406-498 B73/CD1 164.1 22.0 7.5 0 3AR01150:N0406-499 “ 187.5 21.8 8.6 0 8GOQUEEN:N1603-510 “ 197.7 20.1 9.9 0 3GOQUEEN:N1603-511 “ 194.0 19.0 10.2 0 6

Pioneer 34G81 214.5 19.6 10.9 0 0Agway 657 193.3 20.5 9.4 0 4Agway 795 183.6 24.0 7.6 0 3Doeblers 596XY 169.5 20.2 8.4 0 3

Coefficient of variation (%) 10.8 4.9



Work done in 2002…

• S6 progenies planted ear-to-row– Greater emphasis on yield performance– Greater tolerance of stalk rot resistance

• Testcrosses made to:– Previously used public line crosses– Holden’s testers (LH176/LH177, LH198)

developing breeding lines with anthracnose stalk rot resistance from exotic maize germplasm

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