fbs history present future - biowest€¦ · it is of paramount importance to ensure a stable and...
TRANSCRIPT
History Present FutureFBSPurpose of cell culture
In vivoLiving organisms.
In vitro cell cultureModel system for complex
biological tissue and organisms.To culture cells in vitro, a culture medium containing a complex mixture of biological
factors is required.
KnowledgeImprove biology understanding.
FBS?Extremely complex
mixture. Growth-promoting and growth inhibiting
activities necessary to maintain cell lines in an
in vitro environment.
Serum Alternatives
VS
Issue: FBS cannot be chemically defined. Therefore FBS is difficult to replace in research
and prices fluctuate between origins.
We are not even close to replicating the physiological condition for cells.
Serum free alternatives, only for a small fraction of cell lines.
Production process of FBS has remained static over time.
There are no significant alternatives to FBS for most cell lines.
Since replacing use of serum in cell biology applications is not possible, and since it will not
be possible to characterize the chemical composition in the foreseeable future, it is of
paramount importance to investigate (technical) solutions that can ensure production and
supply of serum to scientists that addresses experimental variability.
It is of paramount importance to ensure a stable and consistent production.
Most commonly used and important reagents being used in laboratories.Mammalian cell culture minimizes the need for animal testing.
FBS ?
Scientific need to use chemically defined reagents
to reach consistent and reproducible results.
Challenges in working with FBS
FBSFBS
A century of biomedical sciences
DATA
Insights from millions of scientific articles published over the past 30 years have been impacted by the use of FBS.
Mammalian cell culture use:• Assay purpose/ biology model.• Production purpose.
Academia.
Pharma/Biotech.
Manufacturing.
In vitro diagnostic.