タイピング結果解析 ssp法 -...
TRANSCRIPT
SSP法 (sequence specific primers)
大阪府立急性期・総合医療センター
移植支援検査センター
高山智美
タイピング結果解析
SSP法 実施状況
SSP単独 19施設 輸血関連 6施設
臓器移植 10施設
輸血関連+臓器移植+造血幹移植 2施設
輸血関連+臓器移植+造血幹移植+その他 1施設
その他 1施設
SSP+SSO 2施設 臓器移植 2施設
SSP+SSO+SBT 1施設 臓器移植+造血幹移植 1施設
SSP法 実施状況
キット名 全施設数 (SSP併用 施設数)
low resolution MicroSSP JPN 17 (1)
MicroSSP ABDR 2 (1)
MicroSSP 1L/2L 1
Allset Gold SSP low-res 1
high resolution
MicroSSP Allele Specific 1 (1)
Allset Gold SSP high-res 2 (2)
*複数のSSP検査キットを使用している施設あり
HLAタイピング結果のアリル表記について HLAタイピング結果のアリル表記法と結果報告の原則(2010年度版)
・低解像度(low resolution)のタイピング法 HLA抗原レベルの結果を目的として実施したタイピングの場合は、
第1区域でアリル表記するものとし、第2区域でアリルを表記しない。
・中または高解像度(high resolution)のタイピング法 第2区域までの表記が必要である。
結果
miss assign
?
結果の表記
キット名 LOT Lab.ID HLA-A HLA-B HLA-C
MicroSSP
JPN 007
28D03 A*02:01/02/03/+ A*24:02/03/04/+ B*38:01/02/08/+ B*54:01/04/07/+ C*01:02/03/05/+ C*07:01/02/03/+
28D07 A*02:01/02/03/+ A*24:02/03/04/+ B*38:01/02/08/+ B*54:01/04/07/+ C*01:02/03/05/+ C*07:01/02/03/+
28D23 A*02:01/02/03/+ A*24:02/03/04/+ B*38:01/02/08/+ B*54:01/04/07/+ C*01:02/03/05/+ C*07:01/02/03/+
28D25 A*02:01/02/03/+ A*24:02/03/04/+ B*38:01/02/08/+ B*54:01/04/07/+ C*01:02/03/05/+ C*07:01/02/03/+
28D26 A*02:01/02/03/+ A*24:02/03/04/+ B*38:01/02/08/+ B*54:01/03/05/+ C*01:02/03/05/+ C*07:01/02/03/+
28D30 A*02:01/02/03/+ A*24:02/03/04/+ B*38:01/02/08/+ B*54:01/04/07/+ C*01:02/03/05/+ C*07:01/02/03/+
28D44 A*02:01/02/03/+ A*24:02/03/04/+ B*38:01/02/08/+ B*54:01/04/07/+ C*01:02/03/05/+ C*07:01/02/03/+
28D49 A*02:01/02/03/+ A*24:02/03/04/+ B*38:01/02/08/+ B*54:01/04/07/+ C*01:02/03/05/+ C*07:01/02/03/+
28D52 A*02:01/02/03/+ A*24:02/03/04/+ B*38:01/02/08/+ B*54:01/04/07/+ C*01:02/03/05/+ C*07:01/02/03/+
28D54 A*02:01/02/03/+ A*24:02/03/04/+ B*38:01/02/08/+ B*54:01/04/07/+ C*01:02/03/05/+ C*07:01/02/03/+
28D60 A*02:01/02/03/+ A*24:02/03/04/+ B*38:01/02/08/+ B*54:01/04/07/+ C*01:02/03/05/+ C*07:01/02/03/+
28D61 A*02:01/02/03/+ A*24:02/03/04/+ B*38:01/02/08/+ B*54:01/04/07/+ C*01:02/03/05/+ C*07:01/02/03/+
28D64 A*02:01//+ A*24:02//+ B*38:01/02/08/+ B*54:01/04/07/+
28D66 A*02:01/02/03/+ A*24:02/03/04/+ B*38:01/02/08/+ B*54:01/04/07/+ C*01:02/03/05/+ C*07:01/02/03/+
28D69 A*02:01/02/03/+ A*24:02/03/04/+ B*38:01/02/08/+ B*54:01/04/07/+ C*01:02/03/05/+ C*07:01/02/03/+
28D71 *02:01/02/03/+ *24:02/03/04/+ *38:01/02/08/+ *54:01/04/07/+ *01:02/03/05/+ *07:01/02/03/+
ABDR 009 28D58 A*02:01/02/03/+ A*24:02/03/04/+ B*38:01/02/08/+ B*54:01/08/10/+
010 28D13 A*02:01/02/03/+ A*24:02/03/04/+ B*38:01/02/08/+ B*54:01/10/13/+
1L 010 28D15 A*02:01/02/03/+ A*24:02/03/04/+ B*38:01/02/08/+ B*54:01/13/16/+ C*01:02/03/07/+ C*07:01/02/05/+
Allele Specific B*38/39 B*54-56
002 002
28D41 B*38:02/08/15/+ B*54:01/25/27/+
Allset Gold SSP HLA-A Low Res HLA-B Low Res
022 027
28D19 A*02:03/25/117/+ A*24:02/06/08/+ B*38:01/02/04/+ B*54:01/02/05/+
2801-1
キット名 LOT Lab.ID HLA-A HLA-B HLA-C
MicroSSP
JPN 007
28D03 A*01:01/02/03/+ A*02:01/02/03/+ B*37:01/02/03/+ B*48:01/06/07/+ C*06:02/04/06/+ C*08:01/02/03/+
28D07 A*01:01/02/03/+ A*02:01/02/03/+ B*37:01/02/03/+ B*48:01/06/07/+ C*06:02/04/06/+ C*08:01/02/03/+
28D23 A*01:01/02/03/+ A*02:01/02/03/+ B*37:01/02/03/+ B*48:01/07/09/+ C*06:02/04/06/+ C*08:01/02/03/+
28D25 A*01:01/02/03/+ A*02:01/02/03/+ B*37:01/02/03/+ B*48:01/07/09/+ C*06:02/04/06/+ C*08:01/02/03/+
28D26 A*01:01/02/03/+ A*02:01/02/03/+ B*37:01/02/03/+ B*48:01/06/07/+ C*06:02/04/06/+ C*08:01/02/03/+
28D30 A*01:01/02/03/+ A*02:01/02/03/+ B*37:01/02/03/+ B*48:01/06/07/+ C*06:02/04/06/+ C*08:01/02/03/+
28D44 A*01:01/02/03/+ A*02:01/02/03/+ B*37:01/02/03/+ B*48:01/06/07/+ C*06:02/04/06/+ C*08:01/02/03/+
28D49 A*01:01/02/03/+ A*02:01/02/03/+ B*37:01/02/03/+ B*48:01/06/07/+ C*06:02/04/06/+ C*08:01/02/03/+
28D52 A*01:01/02/03/+ A*02:01/02/03/+ B*37:01/02/03/+ B*48:01/06/07/+ C*06:02/04/06/+ C*08:01/02/03/+
28D54 A*01:01/02/03/+ A*02:01/02/03/+ B*37:01/02/03/+ B*48:01/06/07/+ C*06:02/04/06/+ C*08:01/02/03/+
28D60 A*01:01/02/03/+ A*02:01/02/03/+ B*37:01/02/03/+ B*48:01/07/09/+ C*06:02/04/06/+ C*08:01/02/03/+
28D61 A*01:01/02/03/+ A*02:01/02/03/+ B*37:01/02/03/+ B*48:01/06/07/+ C*06:02/04/06/+ C*08:01/02/03/+
28D64 A*01:01/02/03/+ A*02:01//+ B*37:01//+ B*48:01/06/07/+
28D66 A*01:01/02/03/+ A*02:01/02/03/+ B*37:01/02/03/+ B*48:01/06/07/+ C*06:02/04/06/+ C*08:01/02/03/+
28D69 A*01:01/02/03/+ A*02:01/02/03/+ B*37:01/02/03/+ B*48:01/06/07/+ C*06:02/04/06/+ C*08:01/02/03/+
28D71 *01:01/02/03/+ *02:01/02/03/+ *37:01/02/03/+ *48:01/07/09/+ *06:02/04/06/+ *08:01/02/03/+
ABDR 009 28D58 A*01:01/02/03/+ A*02:01/02/03/+ B*37:01/03/12/+ B*48:01/06/07/+
010 28D13 A*01:01/02/03/+ A*02:01/02/03/+ B*37:01/12/13/+ B*48:01/06/07/+
1L 010 28D15 A*01:01/02/03/+ A*02:01/02/03/+ B*37:01/02/12/+ B*48:01/06/07/+ C*06:02/03/07/+ C*08:01/02/03/+
Allele Specific A*02 C*08
002 003
28D41 A*02:07/219/335/+ 実施なし 実施なし C*08:01/36/42/+
Allset Gold SSP HLA-A Low Res HLA-B Low Res
022 027
28D19 A*01:01/02/03/+ A*02:01/02/04/+ B*37:01/03/10/+ B*48:01/03/04/+
2802-1
キット名 LOT Lab.ID HLA-A HLA-B HLA-C
MicroSSP
JPN 007
28D03 A*03:01/02/03/+ A*11:01/02/03/+ B*44:02/03/05/+ B*51:01/03/07/+ C*03:02/04/05/+ C*05:01/03/05/+
28D07 A*03:01/02/03/+ A*11:01/02/03/+ B*44:02/03/05/+ B*51:01/03/07/+ C*03:02/03/04/+ C*05:01/03/05/+
28D23 A*03:01/02/03/+ A*11:01/02/03/+ B*44:02/03/05/+ B*51:01/03/07/+ C*03:02/04/05/+ C*05:01/03/05/+
28D25 A*03:01/02/03/+ A*11:01/02/03/+ B*44:03/07/13/+ B*51:01/03/07/+ C*03:02/04/05/+ C*05:01/03/05/+
28D26 A*03:01/02/03/+ A*11:01/02/03/+ B*44:02/03/05/+ B*51:01/03/07/+ C*03:02/03/04/+ C*05:01/03/05/+
28D30 A*03:01/02/03/+ A*11:01/02/03/+ B*44:02/03/05/+ B*51:01/03/07/+ C*03:02/03/04/+ C*05:01/03/05/+
28D44 A*03:01/02/03/+ A*11:01/02/03/+ B*44:02/03/05/+ B*51:01/03/07/+ C*03:02/03/04/+ C*05:01/03/05/+
28D49 A*03:01/02/03/+ A*11:01/02/03/+ B*44:02/03/05/+ B*51:01/03/07/+ C*03:02/03/04/+ C*05:01/03/05/+
28D52 A*03:01/02/03/+ A*11:01/02/03/+ B*44:02/03/05/+ B*51:01/03/07 C*03:02/03/04/+ C*05:01/03/05/+
28D54 A*03:01/02/03/+ A*11:01/02/03/+ B*44:02/03/05/+ B*51:01/03/07/+ C*03:02/03/04/+ C*05:01/03/05/+
28D60 A*03:01/02/03/+ A*11:01/02/03/+ B*44:02/03/05/+ B*51:01/03/07/+ C*03:02/04/05/+ C*05:01/03/05/+
28D61 A*03:01/02/03/+ A*11:01/02/03/+ B*44:02/03/05/+ B*51:01/03/07/+ C*03:02/03/04/+ C*05:01/03/05/+
28D64 A*03:01//+ A*11:01//+ B*44:02//+ B*51:01/03/07/+
28D66 A*03/11 A*11/34 B*44:02/03/05/+ B*51:01/03/07/+ C*03:02/03/04/+ C*05:01/03/05/+
28D69 A*03:01/02/03/+ A*11:01/02/03/+ B*44:02/03/05/+ B*51:01/03/07/+ C*03:02/03/04/+ C*05:01/03/05/+
28D71 *03:01/02/03/+ *11:01/02/03/+ *44:03/07/13/+ *51:01/03/07/+ *03:02/04/05/+ *05:01/02/03/+
ABDR 009 28D58 A*03:01/02/03/+ A*11:01/02/03/+ B*51:01/03/07/+ B*44:02/03/06/+
010 28D13 A*03:01/02/04/+ A*11:01/02/03/+ B*44:02/03/06/+ B*51:01/03/07/+
1L 010 28D15 A*03:01/02/04/+ A*11:01/02/03/+ B*44:02/03/06/+ B*51:01/03/07/+ C*05:01/03/04/+ C*03:02/04/05/+
Allset Gold SSP HLA-A Low Res HLA-B Low Res
022 027
28D19 A*03:01/02/03/+ A*11:01/02/03/+ B*44:02/03/04/+ B*51:01/02/03/+
2803-1
キット名 LOT Lab.ID HLA-A HLA-B HLA-C
MicroSSP
JPN 007
28D03 A*24:02/03/04/+ A*33:01/03/04/+ B*15:10/18/37/+ B*40:02/03/08/+ C*03:02/04/05/+ C*07:01/02/04/+
28D07 A*24:02/03/04/+ A*33:01/03/04/+ B*15:10/18/37/+ B*40:02/03/08/+ C*03:02/04/05/+ C*07:01/02/04/+
28D23 A*24:02/03/04/+ A*33:01/03/04/+ B*15:10/18/37/+ B*40:02/03/08/+ C*03:02/04/05/+ C*07:01/02/04/+
28D25 A*24:02/03/04/+ A*33:01/03/04/+ B*15:10/18/37/+ B*40:02/03/08/+ C*03:02/04/05/+ C*07:01/02/04/+
28D26 A*24:02/03/04/+ A*33:01/03/04/+ B*15:10/18/37/+ B*40:02/03/08/+ C*03:02/04/05/+ C*07:01/02/04/+
28D30 A*24:02/03/04/+ A*33:01/03/04/+ B*15:10/18/37/+ B*40:02/03/08/+ C*03:02/04/05/+ C*07:01/02/04/+
28D44 A*24:02/03/04/+ A*33:01/03/04/+ B*15:10/18/37/+ B*40:02/03/08/+ C*03:02/04/05/+ C*07:01/02/04/+
28D49 A*24:02/03/04/+ A*33:01/03/04/+ B*15:10/18/37/+ B*40:02/03/08/+ C*03:02/04/05/+ C*07:01/02/04/+
28D52 A*24:02/03/04/+ A*33:01/03/04/+ B*15:10/18/37/+ B*40:02/03/08/+ C*03:02/04/05/+ C*07:01/02/04/+
28D54 A*24:02/03/04/+ A*33:01/03/04/+ B*15:10/18/37/+ B*40:02/03/08/+ C*03:02/04/05/+ C*07:01/02/04/+
28D58 A*24:02/03/04/+ A*33:01/03/04/+ B*15:10/18/37/+ B*40:02/03/08/+ C*03:02/04/05/+ C*07:01/02/04/+
28D60 A*24:02/03/04/+ A*33:01/03/04/+ B*15:10/18/37/+ B*40:02/03/08/+ C*03:02/04/05/+ C*07:01/02/04/+
28D61 A*24:02/03/04/+ A*33:01/03/04/+ B*15:10/18/37/+ B*40:02/03/08/+ C*03:02/04/05/+ C*07:01/02/04/+
28D64 A*24:02//+ A*33:01/03/04/+ B*15:10/18/37/+ B*40:02/03/08/+
28D66 A*24/33 A*33:01/03/04/+ B*15:10/18/37/+ B*40:02/03/08/+ C*03:02/04/05/+ C*07:01/02/04/+
28D69 A*24:02/03/04/+ A*33:01/03/04/+ B*15:10/18/37/+ B*40:02/03/08/+ C*03:02/04/05/+ C*07:01/02/04/+
28D71 *24:02/03/04/+ *33:01/02/03/+ *15:10/18/37/+ *40:02/03/08/+ *03:02/04/05/+ *07:01/02/04/+
ABDR 009 28D58 A*24:02/03/04/+ A*33:01/03/04/+ B*15:10/18/37/+ B*40:02/03/27/+
010 28D13 A*24:02/03/04/+ A*33:01/03/04/+ B*15:10/18/37/+ B*40:02/03/27/+
1L 010 28D15 A*24:02/03/04/+ A*33:01/03/04/+ B*40:02/03/27/+ B*15:10/18/37/+ C*07:04/11/12/+ C*03:02/04/05/+
Allele Specific C*03 C*07
003 005
28D41 C*03:04/51/54/+ C*07:04/101/139/+
Allset Gold SSP HLA-A Low Res HLA-B Low Res
022 027
28D19 A*24:02/06/08/+ A*33:01/03/04/+ B*15:10/18/37/+ B*40:03/20/267/+
2804-1
2801-2 キット名 LOT Lab.ID HLA-DRB1 HLA-DRB3/4/5 HLA-DQB1
MicroSSP
JPN 007
28D03 DRB1*04:01/02/03/+ DRB1*08:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*03:02/05/07/+ DQB1*06:01/02/03/+
28D07 DRB1*04:01/02/03/+ DRB1*08:01/02/03/+ DRB4*01 - DQB1*03:02/05/07/+ DQB1*06:01/02/03/+
28D23 DRB1*04:01/02/03/+ DRB1*08:01/03/04/+ DRB4*01 - DQB1*03:02/05/07/+ DQB1*06:01/02/03/+
28D25 DRB1*04:01/02/03/+ DRB1*08:01/03/04/+ DRB4*01 - DQB1*03:02/05/07/+ DQB1*06:01/02/03/+
28D26 DRB1*04:01/02/03/+ DRB1*08:01/02/03/+ DRB4*01 - DQB1*03:02/05/07/+ DQB1*06:01/02/03/+
28D30 DRB1*04:01/02/03/+ DRB1*08:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*03:02/05/07/+ DQB1*06:01/02/03/+
28D44 DRB1*04:01/02/03/+ DRB1*08:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*03:02/05/07/+ DQB1*06:01/02/03/+
28D49 DRB1*04:01/02/03/+ DRB1*08:01/02/03/+ DRB4*01 - DQB1*03:02/05/07/+ DQB1*06:01/02/03/+
28D52 DRB1*04:01/02/03/+ DRB1*08:01/02/03/+ DRB4*01 - DQB1*03:02/05/07/+ DQB1*06:01/02/03/+
28D54 DRB1*04:01/02/03/+ DRB1*08:01/02/03/+ DRB4*01 - DQB1*03:02/05/07/+ DQB1*06:01/02/03/+
28D60 DRB1*04:01/02/03/+ DRB1*08:01/03/04/+ DRB4*01 - DQB1*03:02/05/07/+ DQB1*06:01/02/03/+
28D61 DRB1*04:01/02/03/+ DRB1*08:01/02/03/+ DRB4*01 - DQB1*03:02/05/07/+ DQB1*06:01/02/03/+
28D64 DRB1*04/:01//+ DRB1*08:01//+ DRB4* -
28D66 DRB1*04:01/02/03/+ DRB1*08:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*03:02/05/07/+ DQB1*06:01/02/03/+
28D69 DRB1*04:01/02/03/+ DRB1*08:01/02/03/+ DRB4*01 - DQB1*03:02/05/07/+ DQB1*06:01/02/03/+
28D71 *04:01/02/03/+ *08:01/03/04/+ *03:02/05/07/+ *06:01/02/03/+
ABDR 009 28D58 DRB1*04:01/02/03/+ DRB1*08:01/02/03/+ DRB4*01:01/02/03/+ -
010 28D13 DRB1*04:01/02/03/+ DRB1*08:01/02/03/+ DRB4*01 -
2L 007 28D15 DRB1*04:01/02/03/+ DRB1*08:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*03:02/05/07/+ DQB1*06:01/02/03/+
Allset Gold SSP HLA-DR Low Res HLA-DQ Low Res
020 005
28D19 DRB1*04:01/02/03/+ DRB1*08:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*03:02/08/11/+ DQB1*06:01/03/04/+
2802-2 キット名 LOT Lab.ID HLA-DRB1 HLA-DRB3/4/5 HLA-DQB1
MicroSSP
JPN 007
28D03 DRB1*09:01/02/03/+ DRB1*10:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*03:03/06/12/+ DQB1*05:01/02/03/+
28D07 DRB1*09:01/02/03/+ DRB1*10:01/02/03/+ DRB4*01 - DQB1*03:03/06/12/+ DQB1*05:01/02/03/+
28D23 DRB1*09:01/02/03/+ DRB1*10:01/02/03 DRB4*01 - DQB1*03:03/06/12/+ DQB1*05:01/02/03/+
28D25 DRB1*09:01/02/03/+ DRB1*10:01/02/03/+ DRB4*01 - DQB1*03:03/06/12/+ DQB1*05:01/02/03/+
28D26 DRB1*09:01/02/03/+ DRB1*10:01/02/03/+ DRB4*01 - DQB1*03:03/06/12/+ DQB1*05:01/02/03/+
28D30 DRB1*09:01/02/03/+ DRB1*10:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*03:03/06/12/+ DQB1*05:01/02/03/+
28D44 DRB1*09:01/02/03/+ DRB1*10:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*03:03/06/12/+ DQB1*05:01/02/03/+
28D49 DRB1*09:01/02/03/+ DRB1*10:01/02/03/+ DRB4*01 - DQB1*03:03/06/12/+ DQB1*05:01/02/03/+
28D52 DRB1*09:01/02/03/+ DRB1*10:01/02/03/+ DRB4*01 - DQB1*03:03/06/12/+ DQB1*05:01/02/03/+
28D54 DRB1*09:01/02/03/+ DRB1*10:01/02/03/+ DRB4*01 - DQB1*03:03/06/12/+ DQB1*05:01/02/03/+
28D60 DRB1*09:01/02/03/+ DRB1*10:01/02/03 DRB4*01 - DQB1*03:03/06/12/+ DQB1*05:01/02/03/+
28D61 DRB1*09:01/02/03/+ DRB1*10:01/02/03/+ DRB4*01 - DQB1*03:03/06/12/+ DQB1*05:01/02/03/+
28D64 DRB1*09:01/02/03/+ DRB1*10:01/02/03/+ DRB4* -
28D66 DRB1*09:01/02/03/+ DRB1*10:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*03:03/06/12/+ DQB1*05:01/02/03/+
28D69 DRB1*09:01/02/03/+ DRB1*10:01/02/03/+ DRB4*01 - DQB1*03:03/06/12/+ DQB1*05:01/02/03/+
28D71 *09:01/02/03/+ *10:01/02/03/+ *03:03/06/12/+ *05:01/02/03/+
ABDR 009 28D58 DRB1*09:01/02/03/+ DRB1*10:01/02/03/+ DRB4*01:01/02/03/+ -
010 28D13 DRB1*09:01/02/03/+ DRB1*10:01/02/03/+ DRB4*01 -
2L 007 28D15 DRB1*09:01/02/03/+ DRB1*10:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*03:03/06/12/+ DQB1*05:01/02/03/+
Allset Gold SSP HLA-DR Low Res HLA-DQ Low Res
020 005
28D19 DRB1*09:01/02/03/+ DRB1*10:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*03:03/15/20/+ DQB1*05:01/02/03/+
2803-2 キット名 LOT Lab.ID HLA-DRB1 HLA-DRB3/4/5 HLA-DQB1
MicroSSP
JPN 007
28D03 DRB1*04:01/03/04/+ DRB1*15:01/02/03/+ DRB4*01:01/02/03/+ DRB5*01/02 DQB1*04:01/02/04/+ DQB1*06:01/02/03/+
28D07 DRB1*04:01/03/04/+ DRB1*15:01/02/03/+ DRB4*01 DRB5*01/02 DQB1*04:01/02/04/+ DQB1*06:01/02/03/+
28D23 DRB1*04:01/03/04/+ DRB1*15:01/02/03/+ DRB4*01 DRB5*01/02 DQB1*04:01/02/04/+ DQB1*06:01/02/03/+
28D25 DRB1*04:01/03/04/+ DRB1*15:01/02/03/+ DRB4*01 DRB5*01/02 DQB1*04:01/02/04/+ DQB1*06:01/02/03/+
28D26 DRB1*04:01/03/04/+ DRB1*15:01/02/03/+ DRB4*01 DRB5*01/02 DQB1*04:01/02/04/+ DQB1*06:01/02/03/+
28D30 DRB1*04:01/03/04/+ DRB1*15:01/02/03/+ DRB4*01:01/02/03/+ DRB5*01/02 DQB1*04:01/02/04/+ DQB1*06:01/02/03/+
28D44 DRB1*04:01/03/04/+ DRB1*15:01/02/03/+ DRB4*01:01/02/03/+ DRB5*01/02 DQB1*04:01/02/04/+ DQB1*06:01/02/03/+
28D49 DRB1*04:01/03/04/+ DRB1*15:01/02/03/+ DRB4*01 DRB5*01/02 DQB1*04:01/02/04/+ DQB1*06:01/02/03/+
28D52 DRB1*04:01/03/04/+ DRB1*15:01/02/03/+ DRB4*01 DRB5*01/02 DQB1*04:01/02/04/+ DQB1*06:01/02/03/+
28D54 DRB1*04:01/03/04/+ DRB1*15:01/02/03/+ DRB4*01 DRB5*01/02 DQB1*04:01/02/04/+ DQB1*06:01/02/03/+
28D60 DRB1*04:01/03/04/+ DRB1*15:01/02/03/+ DRB4*01 DRB5*01/02 DQB1*04:01/02/04/+ DQB1*06:01/02/03/+
28D61 DRB1*04:01/03/04/+ DRB1*15:01/02/03/+ DRB4*01 DRB5*01/02 DQB1*04:01/02/04/+ DQB1*06:01/02/03/+
28D64 DRB1*04:01/03/04/+ DRB1*15:01/02/03/+ DRB4* DRB5*
28D66 DRB1*04:01/03/04/+ DRB1*15:01/02/03/+ DRB4*01:01/02/03/+ DRB5*01:01/02:02/+ DQB1*04:01/02/04/+ DQB1*06:01/02/03/+
28D69 DRB1*04:01/03/04/+ DRB1*15:01/02/03/+ DRB4*01 DRB5*01/02 DQB1*04:01/02/04/+ DQB1*06:01/02/03/+
28D71 *04:01/03/04/+ *15:01/02/03/+ *04:01/02/04/+ *06:01/02/03/+
ABDR 009 28D58 DRB1*04:01/02/03/+ DRB1*15:01/02/03/+ DRB4*01:01/02/03/+ DRB5*01/02
010 28D13 DRB1*04:01/02/03/+ DRB1*15:01/03/04/+ DRB4*01 DRB5*01/02
2L 007 28D15 DRB1*04:01/02/03/+ DRB1*15:01/02/03/+ DRB4*01:01/02/03/+ DRB5*01/02 DQB1*04:01/02/04 DQB1*06:01/02/03/+
Allset Gold SSP HLA-DR Low Res HLA-DQ Low Res
020 005
28D19 DRB1*04:01/02/03/+ DRB1*15:01/02/03/+ DRB4*01:01/02/03/+ DRB5*01/02 DQB1*04:01/02/04/+ DQB1*06:02/06/10/+
2804-2 キット名 LOT Lab.ID HLA-DRB1 HLA-DRB3/4/5 HLA-DQB1
MicroSSP
JPN 007
28D03 DRB1*04:01/02/03/+ DRB1*14:02/05/06/+ DRB3*01/02/03 DRB4*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*05:01/02/03/+
28D07 DRB1*04:01/02/03/+ DRB1*14:01/02/05/+ DRB3*01/02/03 DRB4*01 DQB1*03:01/04/09/+ DQB1*05:01/02/03/+
28D23 DRB1*04:01/02/03/+ DRB1*14:01/02/05/+ DRB3*01/02/03 DRB4*01 DQB1*03:01/04/09/+ DQB1*05:01/02/03/+
28D25 DRB1*04:01/02/03/+ DRB1*14:01/02/05/+ DRB3*01/02/03 DRB4*01 DQB1*03:01/04/09/+ DQB1*05:01/02/03/+
28D26 DRB1*04:01/02/03/+ DRB1*14:01/02/05/+ DRB4*01 DRB3*01/02/03 DQB1*03:01/04/09/+ DQB1*05:01/02/03/+
28D30 DRB1*04:01/02/03/+ DRB1*14:01/02/05/+ DRB3*01/02/03 DRB4*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*05:01/02/03/+
28D44 DRB1*04:01/02/03/+ DRB1*14:01/02/05/+ DRB3*01/02/03 DRB4*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*05:01/02/03/+
28D49 DRB1*04:01/02/03/+ DRB1*14:01/02/05/+ DRB3*01/02/03 DRB4*01 DQB1*03:01/04/09/+ DQB1*05:01/02/03/+
28D52 DRB1*04:01/02/03/+ DRB1*14:01/02/05/+ DRB3*01/02/03 DRB4*01 DQB1*03:01/04/09/+ DQB1*05:01/02/03/+
28D54 DRB1*04:01/02/03/+ DRB1*14:01/02/05/+ DRB3*01/02/03 DRB4*01 DQB1*03:01/04/09/+ DQB1*05:01/02/03/+
28D58 DRB1*04:01/02/03/+ DRB1*14:01/02/05/+ DRB3*01/02/03 DRB4*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*05:01/02/03/+
28D60 DRB1*04:01/02/03/+ DRB1*14:01/02/05/+ DRB3*01/02/03 DRB4*01 DQB1*03:01/04/09/+ DQB1*05:01/02/03/+
28D61 DRB1*04:01/02/03/+ DRB1*14:01/02/05/+ DRB3*01/02/03 DRB4*01 DQB1*03:01/04/09/+ DQB1*05:01/02/03/+
28D64 DRB1*04:01//+ DRB1*14:01//+ DRB3* DRB4*
28D66 DRB1*04:01/02/03/+ DRB1*14:01/02/04/+ DRB3*01:01/02:01/+ DRB4*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*05:01/02/03/+
28D69 DRB1*04:01/03/04/+ DRB1*14:01/02/05/+ DRB3*01/02/03 DRB4*01 DQB1*03:01/04/09/+ DQB1*05:01/02/03/+
28D71 *04:01/02/03/+ *14:01/02/05/+ *03:01/04/09/+ *05:01/02/03/+
ABDR 009 28D58 DRB1*04:01/02/03/+ DRB1*14:02/03/05/+ DRB3*01/02/03 DRB4*01:01/02/03/+
010 28D13 DRB1*04:01/02/03/+ DRB1*14:02/03/05/+ DRB3*01/02/03 DRB4*01
2L 007 28D15 DRB1*04:01/02/03/+ DRB1*14:02/05/06/+ DRB3*01/02/03 DRB4*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*05:01/02/03/+
Allset Gold SSP HLA-DR Low Res HLA-DQ Low Res
020 005
28D19 DRB1*04:01/02/03/+ DRB1*14:05/43/44/+ DRB3*01/02/03 DRB4*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*05:01/02/03/+
Life Technologies Gold DRB1*14 012 28D41 実施なし DRB1*14:05/91/96/+
DQA1
キット名 LOT Lab.ID HLA-DQA1
Micro SSP DQA1 002 28D41 DQA1*01:03 DQA1*03:01
Life Technologies 006 28D32 DQA1*01:03:01/10 DQA1*03:01
Micro SSP DQA1 002 28D41 DQA1*01:04 DQA1*03:03
Life Technologies 006 28D32 DQA1*01:04 DQA1*03:03
Micro SSP DQA1 002 28D41 DQA1*01:02 DQA1*03:03
Life Technologies 006 28D32 DQA1*01:02/11 DQA1*03:03
Micro SSP DQA1 002 28D41 DQA1*01:05 DQA1*03:02
Life Technologies 006 28D32 DQA1*01:05 DQA1*03:02
2801
2802
2803
2804
・反応データ
・アリル判定
・表記法
・その他
解析方法
反応データ(1)
DRB1*04:01/03/04/+ → DRB1*04:01/02/03/+
false negative
false positive
→プロトコール等の再確認が必要
① false negative
H2804 28D69 :10C (MicroSSP JPN)
反応データ(2)
SSP法によるDNAタイピングの注意点
DNA抽出
PCR増幅
電気泳動
・検体(ヘパリン血不可) ・DNA純度:OD値1.6-1.9 濃度50-100ng/μl
・滅菌蒸留水で希釈
・Taq polymeraseはAmpli Taq推奨
・D-mixの溶液はピンク~紫色(アルカリ) ・PCRプログラムは9600モード
・電源重要
・泳動ゲルは測定ごとに作成
・PCR産物は8連ピペットで素早くアプライ
・泳動時間注意
反応データ(3)
② 転記ミス? false negative?
H2802 28D49 :11A (MicroSSP JPN)
H2804 28D54 :6F (MicroSSP JPN)
アリル判定に影響なし → 報告時の転記ミス??
判定にFusionを使用の場合は、
入力したwellの反応データをCSVファイルにすることが可能
(CSVファイル)
(結果入力シート)
コピー
+
「値だけ」貼り付け
反応データ(4)
③ 陰性は「0」?
28D44、28D60、28D71 : (MicroSSP JPN)
*無反応:インターナルコントロールバンドが出現しない反応
無反応が検出された場合、その検体のタイピング結果は無効
(マイクロSSP HLA DNAタイピングキット使用方法)
MicroSSPの判定について
判定 陽性 陰性 無反応
解析ソフトへの入力 8 1 0
(結果入力シート)
スコアの基準は 明記されていない
MicroSSPの判定 →ワークシート(目視)または解析ソフト
全てのambiguityを網羅するには、
ワークシートだけでは限界あり
アリル判定(1)
④ miss assign 判定にワークシートを使用?
H2803 28D25、28D71 : (MicroSSP JPN)
B*44:03/07/13/+ → B*44:02/03/05/+
アリル判定(2)
反応パターンWell#
2 2 3 4 4 4 7 7
B A F G F C E C
H2803の反応
B*51:01/03/07/+
B*44:02/03/05/+
B*44:03/07/13/+
☜19ページ
☜24ページ
(ワークシート) B*27:127
も同じ反応パターン
アリル判定(3)
⑤ 判定にワークシートを使用? 解析ソフトで使用している各種ファイルのバージョンが古い?
H2803 28D03、28D23、28D25、28D60、28D71
: (MicroSSP JPN)
C*03:02/04/05/+ → C*03:02/03/04/+
解析ソフト使用の場合、updateが必要
(ワークシート) C*05:01:01:01~01:27/ 01:29~03/05~19/21~24/27~ 28/31/33~35/37~41/44:01~ 45/47~57/59~63/65~69/71~ 91N/93~102/104~122; C*08:10
⑥ 欠番
28D71 : (MicroSSP JPN)
表記法(1)
(誤) C*05:01/02/03/+ → C*05:01/03/05/+
C*05:02欠番
判定に解析ソフトを使用すれば、
欠番は表示されない
⑦ Null
28D13 : (MicroSSP ABDR)
(誤) B*54:01/10/13/+ → B*54:01/08/10/+
(Fusionの結果) B*54:01/54:08N/54:10/ 54:13/54:16/54:17/……..
B*54:08Nは「N」をとる
表記法(2)
HLAタイピング結果のアリル表記法と結果報告の原則(2010年度版)
●第1区域に異なるアリルが複数存在する場合 ・第1区域で判別できないアリルが複数存在する場合、最も小さい第1区域の
アリルを記し、その後に「/(スラッシュ)」を入れ表記する。ただし、第1区域で
判別出来ないアリルが複数存在する場合の表記法は原則として用いない。
・第2区域で判別が出来ないアリルが複数存在する場合、アリルを第2区域まで
記し「/(スラッシュ)」でつなぐ。
●第1区域での組み合わせが複数存在し、それらが判別できない場合は、
代表的なアリルに「//+(スラッシュ、スラッシュ、プラス)」をつけて表記する。
第1区域で判別できないアリルが複数存在する場合の表記
表記法(3)
⑧ 第1区域で判別できないアリルが複数存在する場合
28D66 : (MicroSSP JPN)
表記法(4)
検体 HLA-A
H2804 A*24/33 A*33:01/03/04/+
HLA-A
A*24:02/31:08/+ A*33:01/03/04/+ →
A*24:XX1, A*33:XX2
A*31:08, A*33:XX2
A*33:XX3, A*33:XX4
A*33:XX2, A*36:03
表記法(5)
検体 HLA-A
H2803 A*03/11 A*11/34
HLA-A
A*03:01//+ A*11:01//+ →
A*03:XX1, A*03:XX2
A*03:XX1, A*11:XX3
A*03:XX4, A*34:XX5
・・・・ ・・・・ A*11:XX6, A*11:XX7
A*11:XX3, A*30:55
A*11:XX3, A*74:13
・・・・ ・・・・ A*30:55, A*34:XX5
・・・・ ・・・・
XX2=63/88
XX3=01/02/03/04・・・・
DRB3、DRB4、DRB5の表記
(MicroSSP JPN :LOT 007)
反応パターン Well# 11
表記 C B A
DR51 DRB5*01:01~15 DRB5*02:02~06
● DRB5*01:01/02:02/+
or DRB5*01/02
DR52 DRB3*01:01~06/08~13/16 DRB3*02:01~17/19~25/28~29N DRB3*03:01~03
● DRB3*01:01/02:01/+
or DRB3*01/02/03
DR53 DRB4*01:01~07/09 ● DRB4*01:01/02/03/+
or DRB4*01
*ABDR(LOT 009,010)・2L(LOT 007)もプライマーが同じため同様の判定で問題なし
DRB4*02:01N、DRB4*03:01Nは MicroSSPでは陰性
表記法(6)
その他
⑩ 転記ミス? 総合判定と合わない(SSP単独)
H2801 28D26 H2803 28D52
: (MicroSSP JPN) : (MicroSSP JPN)
(SSP判定) (総合判定) B*54:01/03/05/+ → B*54:01/04/07/+
B*51:01/03/07 → B*51:01/03/07/+