fluorescence : one phenomenon, many applications jussi kinnunen, msc (physics), phd student oral...
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Fluorescence : One phenomenon, many
applications Jussi Kinnunen, MSc (Physics), PhD student
Oral presentation
CREATE Gjøvik
There will be:
• Short introduction to fluorescence• Few applications
– Something about bioanalytics– Coloring or whitening merchandise – Arts
• Measurements and data analysis – by using two monochromator –method
Fluorescence : One phenomenon, many applications / Jussi Kinnunen
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Light interaction with matter
When light comes• Absorption
– Time about 10-15 s
• Scattering– Time about 0 s
When light goes• Fluorescence emission
– Lifetime about 10-8(10-10) s
– Lower energy photons than the one(s) absorbed
• Phosphorescence– Lifetime >10-8 s– Lower energy photons
than the one(s) absorbed
• Scattering– Energy if the photon is
close to the incident one(s)
Fluorescence : One phenomenon, many applications / Jussi Kinnunen
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A FP
Fast detection of small quantities of anything• Idea:
– Choose a receptor for the target
– Attach label (reporter) to the receptor
– Illuminate and see • Where is your target
– e.g. cancer cells• Was there any of
your target – e.g. DNA
sequencingFluorescence : One phenomenon, many applications / Jussi Kinnunen
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Masterpiece (Miera, 1950’s), white light and UVPhoto courtesy of Nick Padalino
Fluorescence : One phenomenon, many applications / Jussi Kinnunen
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Measured data
• Donaldson matrix is calculated from the measured data
•The reflectance – On diagonal
•The fluorescence– Off diagonal
Fluorescence : One phenomenon, many applications / Jussi Kinnunen
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Simulation with the scientific brown
Fluorescence : One phenomenon, many applications / Jussi Kinnunen
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Simulation with the scientific brown
Fluorescence : One phenomenon, many applications / Jussi Kinnunen
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Lights
• Simulations under – Tungsten light (green)– Daylight simulator
(blue)– White led (red)– Fluorescent light
(cyan)
Fluorescence : One phenomenon, many applications / Jussi Kinnunen
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Under “smooth” light sources
Halogen light Daylight simulator
Fluorescence : One phenomenon, many applications / Jussi Kinnunen
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reflectance
fluorescence
total
The black curve is measured under
polycromatic light
Under “peaky” light sources
Led light Fluorescent light
Fluorescence : One phenomenon, many applications / Jussi Kinnunen
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reflectance
fluorescence
total
The black curve is measured under
polycromatic light