International Brain Research Organization

IBRO-APRC Advanced School of

Neuroscience

Okazaki, Japan

June 15-26, 2015,

Organized by:

National Institute for Physiological Sciences

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Summary

The IBRO-APRC Advanced School of Neuroscience was held at the National

Institute for Physiological Sciences (NIPS), Okazaki, Japan, from June 15 to 26,

2015. The Advanced School was financially supported by the Asia-Pacific

Regional Committee (APRC) of the International Brain Research Organization

(IBRO) and the NIPS.

Based on the basic concept of IBRO-APRC, we organized the Advanced School

to promote scientific research in the neuroscience field in Asia-Pacific countries.

To this end, this school was designed for young researchers in this field, so that

they could have an excellent opportunity to gain valuable knowledge about

neuroscience and get practical experience in using cutting-edge experimental

methods.

We made a clear announcement on the organizations website stating that the

Advanced School welcomes young applicants who want to contribute to

neuroscience in their own countries. Fifteen students were selected from a total

of 174 applicants. The students selected for participation came from a variety of

countries: Australia (2 persons), India (3), Iran (2), Mongolia (1), Pakistan (1),

Peru (1), Russia (1), Taiwan (1), Thailand (2), and Turkey (1). The participants

consisted of 8 graduate students, 6 postdoctoral fellows and 1 research

associate. Financial support was provide for all of the participants, including

round-trip air fare, expenses during their stay, and accommodations at the

Mishima Lodge on the campus.

The course of the Advanced School consisted of practical experience in the

laboratory and related lectures for two weeks (the program was suffixed).

Twelve courses in laboratory experience were provided. The students actively

gained hands-on experience in using advanced methods and techniques in two

laboratories, for 4 days in each laboratory. They also communicated with faculty

members and graduate students at NIPS during this period. This exposure was

useful as it allowed the participants to get acquainted with experts and develop

international friendships among the next generation in the neuroscience

research field.

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The lectures were highly informative and provided a deep understanding of the

research backgrounds and activities at NIPS. In addition, we provided the

lectures about ethics in science and the regulations for laboratory animals and

animal experimentation.

In response to requests from the students, we arranged laboratory tours almost

every day at NIPS in the evening. The students visited several laboratories,

where they had the opportunity to see the excellent facilities and look at

experiments being conducted using novel techniques.

On the last day, each student gave a presentation of the details of what they

learned and experienced during this short training course. Following the

presentations there was a question and answer session. All of the participants

enjoyed the discussions and interactions in this session.

Overall, we consider that the Advanced School was a great success. The

participation of the students in this school will no doubt contribute to the

promotion of research in their home countries and the improvement of their

carriers in the future. We would like to thank IBRO-APRC for the generous

support.

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List of the students

Dr. Abdulhadi Cihangir Uguz, Suleyman Demirel University, Turkey

Mr. Anthony Ariza, Nagoya University, Japan/Peru

Dr. Ashutosh Kumar, All India Institute of Medical Science, India

Dr. Darambazar Gantulga, National University of Medical Sciences, Mongolia

Mr. Dennis Cheung, University of New South Wales, Australia

Dr. Heena Tabassum, Hamdard University, India

Dr. Huma Jawed, University of Karachi, Pakistan

Ms. Jasneet Parmar, University of New South Wales, Australia

Ms. Masoumeh Ghaemi Jandabi, Tarbiat Modares University, Iran

Ms. Prangtip Potewiratnanond, Chulalongkorn University, Thailand

Mr. Rittinarong Meepong, Chulalongkorn University, Thailand

Dr. Surajit Sahu, DIPAS, India

Ms. Tsui-chin Wang, Mahidol University, Thailand /Taiwan

Ms. Yulia Dembitskaya, Lobachevsky State University of Nizhny Novgorod,

Russia

Dr. Zahra Fatahi Vanani, Shahid Beheshti University of Medical Science, Iran

Organizers

Keiji Imoto, M.D., Ph.D.

Director General,

Professor, NIPS

Junichi Nabekura, M.D., Ph.D.

Duputy Director General,

Professor, NIPS

Tadashi Isa, M.D., Ph.D.

Professor, NIPS

Yumiko Yoshimura, Ph.D.

Professor, NIPS

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Laboratory experiences

During the 2-week period, 12 courses in laboratory experience were provided.

The students intensively experienced advanced methods and techniques in two

laboratories, for 4 days in each laboratory.

The first week

A-1, FRET analysis of GPCR signaling, Kubo Lab

A-2, in situ hybridization, Ikenaka Lab

A-3, Quantitative electron microscopic analysis of neuronal structure, Kawaguchi

Lab

A-4, Analysis of the function of thermosensitive TRP channels with a

patch-clamp method, Tominaga Lab

A-6, Electrophysiological recordings from behaving animals, Nambu Lab

B-6, ECoG as a tool for large-scaled network analysis, Isa Lab

The second week

B-1, Cell biology and biochemistry of cultured hippocampal neurons, Fukata Lab

B-2, Signaling Analyses of cardiovascular adaptation and maladaptation against

hemodynamic load, Nishida Lab

B-3, Laser scanning photostimulation of brain slices, Yoshimura Lab

B-4, In vivo imaging of neurons and glial cells in the brain, Nabekura Lab

B-5, Molecular analysis of feeding behavior, Minokoshi Lab

A-5, Patch clamp recording in vivo, Imoto/ Furue Lab

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[A-1] FRET analysis of GPCR signaling

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[A-2] in situ hybridization

[A-4] Analysis of the function of thermosensitive TRP channels with a

patch-clamp method

[A-5] Patch clamp recording in vivo

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[A-6] Electrophysiological recordings from behaving animals

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[A-6] Electrophysiological recordings from behaving animals

[B-1] Cell biology and biochemistry of cultured hippocampal neurons

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[B-2] Signaling Analyses of cardiovascular adaptation and maladaptation against

hemodynamic load

[B-3] Laser scanning photostimulation of brain slices

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[B-5] Molecular analysis of feeding behavior

[B-6] ECoG as a tool for large-scaled network analysis

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Special lectures

Nine special lectures by

expert scientists were given

to provide the students with

a wide range of

neuroscientific knowledge.

The lectures covered a wide

range of topics, from

molecules to systems.

Dynamic aspects of the function and stoichiometry of ion channel complexes

by Prof. Yoshihiro Kubo

3D reconstruction analysis of neuronal structures using block face SEM by

Asso. Prof. Yoshiyuki Kubota

Remodeling of Neuronal Circuits in development and recovery by Prof. Junichi

Nabekura

Circuit analysis using photostimulation in cortical slices by Prof. Yumiko

Yoshimura

Molecular characterization of feeding behavior by Prof. Yasuhiko Minokoshi

Recording, decoding and manipulating the large-scaled network activity for

causal neuroscience by Prof. Tadashi Isa

Cortico-basal ganglia loop and movement disorders by Prof. Atsushi Nambu

Inter-individual neural synchronization during social interaction by Prof.

Norihiro Sadato

Multivariate decoding of visual representation from neural activity by Assi. Prof.

Naokazu Goda

Lecture by Prof. Isa

Lecture by Prof. Nambu

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Presentation session

Remarks by Prof. Imoto,

Director-general of NIPS

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IBRO-APRC advanced School Schedule

June 15 (Monday)

Time Place

10:45 Registration Myodaiji NIPS Main

building 1F

13:30-13:50 Opening remarks

Prof. Keiji Imoto, Director-General of NIPS

Myodaiji NIPS Main

building 1F, Lecture room

13:50-14:00 Introduction of the School

Prof. Yumiko Yoshimura

14:00-15:00

Ethics in science---what to know and how to

behave as a scientist

Prof. Tadashi Isa

15:00-15:30 The Regulations for Laboratory Animal and

Animal Experimentation in Japan

Break

15:40-17:40

Special Lecture 1 Prof. Yoshihiro Kubo

Dynamic aspects of the function and stoichiometry of ion channel complexes

Break

18:00 Welcome party Cafeteria IF

June 16 (Tuesday)

Time Place

8:30-10:30

Special Lecture 2 Asso. Prof. Yoshiyuki Kubota

3D reconstruction analysis of neuronal structures

using block face SEM

Myodaiji NIPS

Main building 1F,

Lecture room

10:30- Laboratory experiences Group A

Shuttle Bus (Myodaiji Yamate) Dep. 10:40 Arr. 10:50

June 17 (Wednesday)

Time Place

8:30-10:30

Special Lecture 3 Prof. Junichi Nabekura

Remodeling of Neuronal Circuits in development and

recovery

Myodaiji NIPS

Main building 1F,

Seminar room

10:30- Laboratory experiences Group A

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June 18 (Thursday)

Time Place

8:30-10:30

Special Lecture 4 Prof. Yumiko Yoshimura

Circuit analysis using photostimulation in cortical

slices

Myodaiji NIPS

Main building 1F,

Lecture room

10:30- Laboratory experiences Group A

June 19 (Friday)

Time Place

8:30-10:30 Special Lecture 5 Prof. Yasuhiko Minokoshi

Molecular characterization of feeding behavior

Myodaiji NIPS

Main building 1F,

Lecture room

10:30- Laboratory experiences Group A

-----------------------------

June 22 (Monday)

Time Place

8:30-10:30

Special Lecture 6 Prof. Tadashi Isa

Recording, decoding and manipulating the

large-scaled network activity for causal neuroscience

Myodaiji NIPS

Main building 1F,

Lecture room

10:30- Laboratory experiences Group B

June 23 (Tuesday)

Time Place

8:30-10:30 Special Lecture 7 Prof. Atsushi Nambu

Cortico-basal ganglia loop and movement disorders

Myodaiji NIPS

Main building 1F,

Seminar room

10:30- Laboratory experiences Group B

June 24 (Wednesday)

Time Place

8:30-10:30

Special Lecture 8 Prof. Norihiro Sadato

Inter-individual neural synchronization during social

interaction

Myodaiji NIPS

Main building 1F,

Seminar room

10:30- Laboratory experiences Group B

June 25 (Thursday)

Time Place

8:30-10:30

Special Lecture 9 Assi. Prof. Naokazu Goda

Multivariate decoding of visual representation from

neural activity

Myodaiji NIPS

Main building 1F,

Seminar room

10:30- Laboratory experiences Group B

June 26 (Friday)

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Project presentation session

PlaceMyodaiji NIPS Main building 1F, Seminar room

ChairAssi. Prof. Toshio Miyashita

Time Presenter Group AB

9:00-9:10 Ms. Jasneet Parmar Nambu labNabekura lab

9:10-9:20 Mr. Ashutosh Kumar Nambu labFukata lab

9:20-9:30 Ms. Masoumeh Ghaemi Jandabi Nambu labMinokoshi lab

9:30-9:40 Ms. Tsui-chin Wang Ikenaka LabNabekura lab

9:40-9:50 Mr. Surajit Sahu Ikenaka LabNabekura lab

Break

10:00-10:10 Dr. Heena Tabassum Ikenaka LabFukata lab

10:10-10:20 Mr. Anthony Ariza Kubo LabFukata lab

10:20-10:30 Dr. Zahra Fatahi Vanani Kawaguchi labYoshimura lab

10:30-10:40 Mr. Rittinarong Meepong Kawaguchi labImoto&Furue lab

10:40-10:50 Ms. Yulia Dembitskaya Isa labImoto&Furue lab

Break

11:00-11:10 Mr. Dennis Cheung Isa labYoshimura lab

11:10-11:20 Dr. Huma Jawed Kubo LabNishida lab

11:20-11:30 Dr. Abdulhadi Cihangir Uguz Tominaga labNishida lab

11:30-11:40 Dr. Darambazar Gantulga Tominaga labNishida lab

11:40-11:50 Ms. Prangtip Potewiratnanond Tominaga labMinokoshi lab

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Laboratory experiences

M: Myodaiji NIPS main building, Y2: Yamate second building, Y3: Yamate

third building

GroupA (June 16-19)

Course

Loc. Title Divisions Students

A-1

(M-3F)

FRET analysis of GPCR

signaling Kubo Lab

Mr. Anthony Ariza Dr. Huma Jawed

A-2

(Y2-7F) in situ hybridization

Ikenaka

Lab

Dr. Heena Tabassum

Mr. Surajit Sahu

Ms. Tsui-chin Wang

A-3

(Y2-5F)

Quantitative electron

microscopic analysis of

neuronal structure

Kawaguchi

Lab

Mr. Rittinarong Meepong

Dr. Zahra Fatahi Vanani

A-4

(Y2-6F)

Analysis of the function of

thermosensitive TRP

channels with a

patch-clamp method

Tominaga

Lab

Dr. Abdulhadi Cihangir Uguz

Dr. Darambazar Gantulga

Ms. Prangtip Potewiratnanond

A-6

(M-7F)

Electrophysiological

recordings from behaving

animals

Nambu Lab

Mr. Ashutosh Kumar

Ms. Jasneet Parmar Ms. Masoumeh Ghaemi Jandabi

B-6

(M-6F)

ECoG as a tool for

large-scaled network

analysis

Isa Lab Mr. Dennis Cheung

Ms. Yulia Dembitskaya

GroupB (June 22-25)

Course

Loc. Title Divisions Students

B-1

(Y3-8F)

Cell biology and

biochemistry of cultured

hippocampal neurons

Fukata Lab

Mr. Anthony Ariza

Mr. Ashutosh Kumar

Dr. Heena Tabassum

B-2

(Y3-2F)

Signaling Analyses of

cardiovascular adaptation

and maladaptation against

hemodynamic load

Nishida

Lab

Dr. Abdulhadi Cihangir Uguz Dr. Darambazar Gantulga

Dr. Huma Jawed

B-3

(M-3F)

Laser scanning

photostimulation of brain

slices

Yoshimura

Lab

Mr. Dennis Cheung

Dr. Zahra Fatahi Vanani

B-4

(M-5F)

In vivo imaging of neurons

and glial cells in the brain

Nabekura

Lab

Ms. Jasneet Parmar

Mr. Surajit Sahu

Ms. Tsui-chin Wang

B-5

(M-4F)

Molecular analysis of

feeding behavior

Minokoshi

Lab

Ms. Masoumeh Ghaemi Jandabi Ms. Prangtip Potewiratnanond

A-5

(Y3-9F)

Patch clamp recording in

vivo

Imoto/

Furue Lab

Mr. Rittinarong Meepong Ms. Yulia Dembitskaya