IGEM TEAM EPF-LAUSANNE

WHAT IS IGEM?

International Genetically Engineered Machine

Having received a kit of biological parts from the iGEM Registry, we start to build our biological systems over the summer.

The perspective of the Jamboree in November at MIT and the project design is exceptionally motivating, in addition to the fact that this is a good learning experience.

OUR TEAM & OUR SCHOOL

THE PROJECT: WET LAB PART

THE PROJECT: WET LAB PART

Cloning Parts:

using the BioBrick protocol of Ginkgo Bioworks

THE PROJECT: MAKING A PART

THE PROJECT: WET LAB PART

The BphP / PpsR system

THE PROJECT: MODELING PART-AIM

Molecular dynamics (MD) simulation of the photosensitive protein domain on computer for two protein states:-The light activated state-The ground state (protein without light excitation)

Study of both state stability (statistical analysis)

Determination of amino acids that could be mutated to enhance stability of light activated state

Mutation of the amino acids and test the switch in vivo

Goal: Enhance the binary effect of the light switch

First nanosecond MD simulation:

THE PROJECT: MODELING PART-PRELIMINARY RESULTS

THE PROJECT: APPLICATIONS

Merck-Serono interests: using light to induce gene transcription in

mammalian cells

Our Mentors:

Sebastian Maerkl Laboratory of Biological Network Characterization (STI/SV)

Bart Deplancke Laboratory of Systems Biology and Genetics

Matteo Dal Peraro Laboratory for Biomolecular Modeling

Felix Naef Computational systems biology

THANK YOU!