igem team epf-lausanne
DESCRIPTION
iGEM TEAM EPF-LAUSANNE. International Genetically Engineered Machine. OUR TEAM & OUR SCHOOL. Carine. Christian. Tu. Nathalie. Nicolas. Rafael. Nicolas. Gabriela. 10 undergraduate students 6 advisors. Heidi. Sebastian. Caroline. Mélanie. - PowerPoint PPT PresentationTRANSCRIPT
iGEM TEAM EPF-LAUSANNE
International Genetically Engineered Machine
OUR TEAM & OUR SCHOOL
NicolasChristian
GabrielaTu Nathalie
Carine
Nicolas Rafael
Heidi Sebastian Caroline Mélanie
10 undergraduate students
6 advisors
Specific Aims:
1. To develop strategies to regulate gene expression using light as a stimulus.
2. To use computational tools to optimize the selected strategies.
Our approach: To utilize defined molecular systems involved in gene regulation.
The activity of the molecules involved should be amenable to regulation by light-induced changes in protein conformation.
Gene Regulation by Modulating Protein Conformational Changes:
The LovTAP system
Features:
- Light-activated
- Reversible
- Synthetic system
RO#1
RO#2
Gene Regulation by Modulating Protein Conformational Changes:
Experimental Design
Experimental Design: iGEM TOOLS
Experimental Design: Cloning Strategy
Results: Read-out n°1 characterization
RO#1 without Tryptophan RO#1 with Tryptopan
Results: Entire system (LovTAP + read-out n°2)
Specific Aim 2 : To use computational tools to optimize the selected strategies
Desired Features:
• Increased lifetime of the light-activated protein
• Enhanced affinity of the binding of the light-activated protein to the DNA
The LovTAP system
Experimental Design: Insight from Molecular Dynamics and Biochemical Modeling
Applications
Specific and temporal induction of gene transcription in mammalian cells
Coupling with gene recombination systems
Sebastian Maerkl : Laboratory of Biological Network Characterization
Bart Deplancke : Laboratory of Systems Biology and Genetics
Matteo Dal Peraro : Laboratory for Biomolecular Modeling
Felix Naef : Computational systems biology
Acknowledgements
Thank You For Your Attention