instrumentation handbook 2010-2011

8/2/2019 Instrumentation Handbook 2010-2011

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RESEARCH

INSTRUMENTATION

HANDBOOK

PO LICIE S AND FEE S

FOR INSTRUMENT USE

E F F E CT I VE : MAY 1, 2 01 0 APRI L 30 , 20 11

RE VI SI O N : 1

W I L F R I D L A U R I E R U N I V E R S I T Y


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RESEARCH INSTRUMENTATION HANDBOOK 1

TABLE O F CO N T E N T S

G E N E R A L U S E R G U I D E L I N E S

LABORATORY SAFETY 2

MULTI-USER INSTRUMENTS 3

AMINCO/THERMO FRENCH PRESS 3

BECKMAN AVANTI J30I CENTRIFUGE 3

CARY 50 UV-VIS SPECTROMETER 4

CARY ECLSIPSE FLUORESCENCE SPECTROMETER 4

CENTRIVAP CENTRIFUGAL CONCENTRATOR 5

DNR BIS 303 IMAGING SYSTEM 5

DYNEX MRX REVELATION PLATE READER 5EPPENDORF 5804R REFRIDGERATED CENTRIFUGE 5

GLASSCOUNTOUR DRY SOLVENT SYSTEM 6

INSTA-ICE 475 DRY ICE BLOCK MAKER 6

MIELE GLASSWARE WASHER 6

MILLI-Q SYNTHESIS A-10 7

STERIS PREVACUUM STERILIZER 7

ULTRALOW -80 FREEZERS 7

SPECIALIZED INSTRUMENTS 8

CHROMATOGRAPHY 8

TITRATION 9

SPECTROSCOPY 9

DNA ANALYSIS 10

MICROSCOPY 12

OTHER SPECIALIZED EQUIPMENT 13

T R A I N I N G AN D U S E R F E E S

USER TRAINING AND CERTIFICATION 15

INTERNAL USER FEES 15

EXTERNAL USER FEES 17

TECHNICAL PROBLEMS AND EQUIPMENT DAMAGE 18


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PUR POSEThis Instrumentation Handbook has been developed to promote efficient and safe

instrument operation and facilitate research collaboration within the Faculty of Science.

This handbook lists the instruments currently available for research use and describesthe policies and fees governing the use of these instruments. Some of the instruments

listed are owned by the Faculty of Science, and are referred to as multi-user

instruments. The other instruments listed, termed specialized instruments, are

controlled by specific researchers, and are available to other users at the discretion of

the researcher.

G E N E RAL U SE R G U I D E L I N E S

LABORATO RY SAFE TY

Comprehensive guidelines for laboratory safety,1 biosafety,2 and radiation safety3 have

been developed by the Environmental/Occupational Health and Safety Office, and are

available on-line. Specific guidelines for the safe use of each multi-user instrument are

provided in the User Guidelines and Standard Operating Procedure (SOP) for thatspecific instrument. The SOPs are also available on-line.4 In all cases, individuals using

any laboratory instrument must be aware of the principles of operation, associated

hazards, personal protective equipment, and related waste disposal before commencing

use. The Instrumentation Technician provides training for many of the instruments listed

in this handbook (see the Training section for further details).

Laboratory cleanliness must be maintained by everyone using a multi-user or specialized

instrument. The laboratory space around the instrument must remain free of dust, debris,

and personal items. Each user is responsible for cleaning the area around an instrument

after use.

1 Laboratory Health and Safety Manual: www.wlu.ca/documents/23120/Laboratory_Health_%26_Safety_Manual.pdf2

Biosafety Manual: www.wlu.ca/documents/23632/Biosafety_Manual_March_15_2007_Final.pdf3

Radiation Safety Manual: www.wlu.ca/documents/14021/RSM_Jan_2010.pdf4 Link for instrument SOPs: www.wlu.ca/page.php?grp_id=2106&p=10255


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MULTI-US ER INS TR UMENTS

The multi-user instruments purchased by the Faculty of Science are available to all

internal users, and to external users as determined by the Instrumentation Technician.

Specialized instruments purchased under individual faculty grants are available to

internal and external users at the discretion of the researcher (referred to as the principal

investigator or PI).

All of the multi-user instruments listed in this section are available for use on a first

come, first serve basis, except the fluorescence spectrometer. Twenty-four hours notice

is required to use the fluorescence spectrometer because some samples are time

sensitive, and may take up to 8 hours to analyze. A sign-up sheet is available in the

fluorescence spectrometer log book.

Data recording and back-up is the sole responsibility of the user. Data should betransferred to another computer for safe-keeping, rather than stored on the computer

that controls a multi-user instrument.

AMINCO/ THERMO FRENCH PRESS

PI: Dr. Art Szabo

The French Press can be used for cell disruption or subcellular

fractionation, enzyme preparation, bacterial and plant tissue

degradation, and recovery of intercellular proteins and nucleic acid. The

cell operates at pressures up to 40,000 psi and can contain a maximum

volume of 35 mL.

BECKMAN AVANTI J30I CENTRIFUGE

PI: Dr. Art Szabo/Dr. Matt Smith/Science

The Avanti J-30I centrifuge can be used to achieve very fast centrifugal

separations. Swinging-bucket and fixed-angle rotors provide maximum

separation forces in excess of 100,000 x g at speeds up to 30,000 RPM.There are three different rotors that may be used in this centrifuge: the

JLA10.500 fixed angle rotor (holds 6 x 500 mL flat bottomed vessels);

the JA-30.50 Ti fixed angle rotor (holds 8 x 50 mL round bottomed

tubes); and the JS 13.1 swinging bucket rotor.


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CARY50 UV-VIS SPECTROMETER

PI: Dr. Art Szabo

The Cary 50 can be used to measure the absorbance / transmittance /

optical density of a variety of samples using the following software

applications:

Advanced Reads: Read multiple samples in a single run.

Concentration: Quantitative analysis using a calibration curve.

Color: Measure and calculate transmitted or reflected sample colour.

Enzyme Kinetics: Kinetics analysis using Michaelis-Menten equation.

Kinetics: Time-based single or multicell measurements.

RNA / DNA: Asses the amount, type and purity of nucleic acid.

Scan: Measure absorbance across a range of wavelengths.

Scanning Kinetics: Determine kinetics curves from a set of spectra.

Simple Reads: Measure absorbance readings at a single wavelength.

Thermal: Absorbance measurements with temperature variation, often

used for DNA melts.

CARYECLIPSE FLUORESCENCE SPECTROMETER

PI: Dr. Art Szabo

The Cary Eclipse can be used to measure sample fluorescence,

phosphorescence, or bioluminescence using the following software

applications:

Advanced Reads: Read multiple samples in a single run.

Concentration: Perform quantitative analysis with a calibration curve.

Kinetics: Measure the change in emission intensity over time.

Lifetimes: Measure phosphorescence lifetimes or delayed-

fluorescence lifetimes.

Scan: scan the emission or excitation spectrum of samples across a

wavelength or wavenumber range.

Simple Reads: Measure a sample at a single wavelength

Thermal: Perform thermal analyses on DNA using the 4-position

multicell Peltier accessory.


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CENTRIVAP CENTRIFUGAL CONCENTRATOR

PI: Dr. Art Szabo

The Centrivap centrifugal concentrator uses centrifugal force, heat, and

vacuum to remove water from multiple small samples. The rotor can

accommodate up to 132 x 1.5 mL samples in Eppendorf tubes, 18 x 15

mL samples, or 12 x 50 mL samples. Solvents cannot be removed

using this system.

DNR BIS303 IMAGING SYSTEM

PI: Faculty of Science

The BIS 303 PC is a bio-imaging system used to image

Chemiluminescence on agarose and polyacrylamide gels, membranes,

films, blots, TLC plates, and autoradiographs. Gels and plates of up to12 x 25 cm can be imaged using a cooled 1.75 MP CCD camera

connected to the external PC. The final images can be saved onto a disk

or memory key.

DYNEX MRX REVELATION PLATE READER


The MRX Revelation reads sample optical density in a 96-well plate and

can be used for endpoint, kinetic and tissue culture agglutinationassays. This instrument has a spectral range of 405 to 850 nm Up to six

filters can be used in single or dual wavelength reading modes.

EPPENDORF 5804R REFRIDGERATED CENTRIFUGE


This refrigerated benchtop centrifuge is equipped with a swinging

bucket A-4-44 rotor and adaptors which can accommodate 2 mL and 15mL tubes and operate at speeds up to 5000 RPM. The temperature can

be set between -9 oC to 40 oC, and the centrifuge can remain in standby

to maintain temperature when not in use. There are ten different

acceleration and deceleration rates for sensitive samples, and up to 34

individual user settings can be programmed.


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GLASSCOUNTOUR DRY SOLVENT SYSTEM


The dry solvent system provides a safe alternative to solvent distillation,

and produces dry high purity solvents on demand. Solvents pass

through activated columns under low pressure to remove trace

impurities and water. The dry solvents currently available are

tetrahydrofuran, ether, dichloromethane, toluene, hexane, acetonitrile,

dimethylformamide, and methanol.

INSTA-ICE 475D RY ICE BLOCK MAKER


The 475 Ice block maker can be used to produce small dry ice blocks,

up to 8.5 x 6.7 x 18.4 cm. Dry ice is often used for organic chemistry

applications or shipment of sensitive samples.

MIELE GLASSWARE WASHER


The Miele general purpose laboratory washer is equipped with three

wash routines, and can accommodate a variety of baskets and inserts

specifically designed for laboratory glassware. An injection basket isavailable for narrow mouth glassware and standard baskets should be

used for wide mouthed items. The wash and DI rinse water are heated

to 70C to ensure proper removal of both the contaminant and detergent

residues.


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MILLI-Q SYNTHESIS A-10


The Milli-Q A-10 Synthesis provides high quality ultrapure water for a

variety of sensitive applications. Milli-Q water has a resistivity of 18.2

M.cm @ 25C, and less than 10 ppb TOC. The main components of

the Milli-Q system are as follows:

Q-Gard purification pack for the removal of organics and ions from the

building DI feedwater.

Quantum EX cartridge for additional organic removal to trace levels

using mixed acid and base resins and activated carbon.

Millipak 0.22 m membrane filter.

UV lamp to destroy organic molecules and reduce TOC levels to < 10

ppb.

Pyrogard 5000 ultrafiltration cartridge for the production of pyrogen-

and nuclease-free ultrapure water (< 0.001 EU/mL), which is

particularly important for life science or molecular biology applications.

STERIS PREVACUUM STERILIZER


This autoclave is designed for fast, efficient sterilization of heat- and

moisture-stable materials including media, glassware, biological waste,

and soils. Various liquid, gravity, and prevacuum cycles are available.The autoclave is tested regularly by the Instrumentation Technician to

ensure proper operation. Users are responsible for testing their own

sterilization procedures, particularly with regard to waste.

ULTRA-LOW -80 FREEZERS

PI: Dr. Matt Smith, Dr, Lucy Lee, Dr. Art Szabo

There are several ultra-low freezers in the Science Research building.

These freezers can only be used after consultation with the appropriatePI. The samples in these freezers are very sensitive and valuable, and

brief training is therefore required before using any of the ultra-low

freezers.


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SP E C I AL I Z E D I N S T RU ME N T S

A wide variety of specialized and complex equipment is present within the Faculty of

Science. Some of the specialized instruments purchased under individual faculty grants

are available to internal and external users at the discretion of the PI. If this use is not

part of an official collaboration, user and training fees may apply. Users are also

responsible for the costs of consumables, as determined by the PI. The following

instruments are considered specialized equipment:

CHROMATOGRAPHY

VARIAN SATURN 2000 GC/ MS

A GC/MS can be used to separate a wide variety of compounds based on volatility and

polarity, depending on the column used. This technique is particularly useful for small

quantity samples. The analyte of interest must be volatile and stable at high

temperatures (i.e. up to ~350 oC). The Varian GC-MS is equipped with a CP8200

autosampler which can hold up to 48 vials, and allows for liquid, solid phase

microextraction (SPME), or ambient headspace injection of volumes using a 10 l or 100

l syringe. The 1079 programmable temperature vaporizing (PVT) injector can be used

for split and splitless, temperature-ramped splitless, large volume (LVI), and cold on-

column injection. Fractions exiting the column can be detected using flame ionization

(FID), electron capture (ECD), and/or mass spectrometry.

PIs: Dr. Frdrique Guinel, Dr. Mary Ann Fieldes

WATERS DELTA 600 H PLC (2)

There are two Waters Delta HPLC systems in the Faculty of Science. Combined, these

systems offer semi-preparative and analytical capabilities; a photodiode array;

absorbance, or refractive index detection; an autosampler; and fraction collection.

Separations can be run in both isocratic and gradient mode at flow rates up to 20 ml/min

depending on the column. Waters Empower software can be used to control runs and

analyze data.

PIs: Dr. Masoud Jelokhani-Niaraki, Dr. Steve MacNeil

WATERS MICROMASS ZQ LC-MSThis HPLC-MS system is equipped with two Waters 515 pumps, an absorbance

detector, a column oven, and the Micromass ZQ single quadrupole mass spectrometer.

The ZQ can ionize samples using either electrospray ionization (+/- ESI) or atmospheric

pressure chemical ionization (APCI). Data collection and analysis are conducted using

Masslynx 4.0 software.

PI: Dr. Masoud Jelokhani-Niaraki


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TITRATION

METTLER TOLEDO DL32KARL FISHER TITRATOR

The DL32 is a coulometric KF titrator which can be used to determine water contents in

small sample volumes. The DL32 is ideal for water contents in the range of 1 ppm to 5%.

PI: Dr. Steve MacNeil

MICROCAL VP-ITC ISOTHERMAL TITRATOR MICROCALORIMETER

The VP-ITC can be used to study reaction thermodynamics, binding constants, and

stoichiometry. This instrument measures the heat released or absorbed as a reaction

occurs, and can be used to monitor reactions that are initiated by a binding component,

including: antigen-antibody, DNA-drug, and protein-ligand reactions. Typical injection

volumes are between 2-10 L, and sample/macromolecule concentrations should be

between 5 M 1 mM.


TANAGER DUAL PH METER AND TITRIMETER

This automatic titrator is interfaced with a modular spectrometer system and can be used

to run controlled reactions while monitoring a variety of physical and chemical

parameters.

PI: Dr. Scott Smith

SPECTROSCOPY

AVIV CIRCULAR DICHROSIM SPECTROMETER

The circular dichroism (CD) spectrometer can be used to assess the conformation of

various sample types, including DNA and proteins. This instrument can be used to

conduct wavelength, temperature, kinetic, and titration experiments. CD spectra can be

recorded as a function of wavelength, time, temperature, pH, or concentration.


MODULAR FIBER OPTIC SPECTROMETER

This modular fiber optic system includes a 1m light path for colorimetery, a UV-Vis

absorbance fiber optic probe, and a fluorescence fiber optic probe. The spectrometer

components are connected to a Tanager autotitration system (see above).PI: Dr. Scott Smith

MOLECULAR DEVICES SPECTRAMAX M2 MICROPLATE SPECTROPHOTOMETER

This microplate reader is equipped with dual-monochromators and dual-mode cuvette

ports for absorbance and fluorescence intensity detection. Endpoint, kinetic, spectrum,

and area-well scanning assays can be conducted on 6- to 384-well microplates.

PI: Dr. Jim McGeer


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VARIAN SPECTRA 880 ATOMIC ABSORPTION SPECTROMETER

The Varian 880 AAS is equipped with a sample introduction system pump system

(SIPS), GTA100 graphite tube atomizer, and deuterium continuum source background

correction. The SIPS system provides multi-point calibration from a single standard and

fast on-line dilution of over-range samples. The GTA100 features a Constant

Temperature Zone (CTZ) design which provides low detection limits, excellentsensitivities, long tube lifetimes and reduced memory effects.

PI: Dr. Jim McGeer

PERKIN ELMER MODEL 3100AAS ATOMIC ABSORPTION SPECTROMETER

This AAS is equipped with the lamps required to do all major cations and many heavy

metals. The detection limits for most elements is low ppm (i.e. 0.30 ppm), however,

heavy metals in natural waters are often difficult to detect because they are usually in the

low ppb range.

PIs: Chemistry, Co-supervised with Geography and Environmental Studies

SIMPLE FIBER OPTIC RAMAN SPECTROMETER (RSI)

Raman spectroscopy can be used to study a wide variety of sample types based on a

change in polarizability due to vibrational motion following absorption of a photon.

PI: Dr. Vladimir Kitaev

DN A ANALYSIS

BIORAD ICYCLER IQ REAL TIME PCR MACHINE :

This real-time PCR instrument enables monitoring while the reaction progresses,

allowing for more accurate quantification of the starting template and elimination of post-

PCR processing. The iCycler accepts 96 well plates and has filters installed for FAM490,

Cy3/Vic/Hex/Tet and Rox/texas red. The light source is a tungsten halogen lamp, and

the detector is a CCD camera. Protocol development and data analysis are controlled by

the iCycler software.

PI: Dr. Robin Slawson

BIO-RAD MOLECULAR I MAGER GEL-DOC XR

The Gel Doc system is equipped with a camera, UV- and white-light illumination, and afilter for ethidium bromide imaging. The detection range covers 3 orders of magnitude.

Colony counting and 1-D gel quantification and analysis are conducted using Quantity 1

software. All data must be saved on a memory key and transferred to a separate

computer (do not store your data on the computer connected to the Gel Doc). Ethidium

bromide very hazardous and a known mutagen: all ethidium bromide stained

products must be brought in the lab in a closed container.



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BIORAD PERSONAL FX MOLECULAR IMAGER PHOSPHORIMAGER

This phosphorimager detects radio-labeled DNA and protein molecules using phosphor

screens. The imager accommodates both large (35 x 43 cm) and small (20 x 25 cm)

storage phosphor screens. Storage phosphor technology offers the high isotopic

sensitivity, with exposure times typically 1/10 that of film. Samples can be labeled using

the radioisotopes 32P, 33P, 35S, 14C and 3H. The information obtained from the imager can

be used to assess protein metabolism, gene expression, and nucleic acid sequences.

Any work involving radioactivity must first be approved by the Environmental/

Occupational Health and Safety Office.

PI: Dr. Matt Smith

CBS SCIENTIFIC DGGE GEL SYSTE M

Denaturing gradient gel electrophoresis can be used to separate PCR products based

on sequence differences and can be used to identify single base changes and

polymorphisms in DNA. This system includes a pump and gradient maker to preparegels, allows for buffer heating and agitation, and can run up to 4 gels simultaneously.

The generator can power up to 300 V.


LICOR 4300 DNA SEQUENCER

The Licor sequencer can be used for DNA sequencing, microsatellite analysis, and

amplified fragment length polymorphism (AFLP) analysis. This instrument is capable of

reading up to 1000-1200 bp with very high accuracy using a dual laser system. Up to 64

samples can be loaded in one run. The software can be used to view the electrophoresisresults, make manual changes, and to align sequences.

PIs: Dr. Scott Ramsay, Dr. Matt Smith


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MICROSCOPY

LEICA DMR HC UPRIGHT BRIGHTFIELD MICROSCOPE

This is a research quality microscope with brightfield and differential interference

contrast capability. The microscope is equipped with a digital imaging system.

PI: Dr. Paul Mallet

OLYMPUS BX-51OPTICAL MICROSCOPE

This optical microscope is capable of up to 1500X magnification. It is equipped with dry

lenses, which can be coupled to a sensitive UV-Vis spectrometer for

microspectroscopy (~10 micron by 10 micron areas).

PI: Dr. Vladimir Kitaev

OLYMPUS FLUOVIE W FV1000 CONFOCAL LASER SCANNING MICROSCOPE

This inverted confocal laser scanning microscope can be used for 3-D imaging, time

course experiments, energy transfer visualization, and photobleaching experimentsusing both live and mounted specimens. The system is equipped with three lasers: a

multi argon-ion laser (457, 488, and 515 nm), a helium-neon (green) laser (543 nm),

and two diode lasers (643 and 405 nm). The high quality optics include 5 objective

lenses: 10x, 20x, 40x, 60x (oil immersion), and 100x (oil immersion). The user friendly

software is used for image acquisition and simple analysis. Images can also be viewed

on a personal computer using a limited freeware version of the software. This system

requires #1 (0.17 mm) coverslips for all objectives except the 40x objective, which has

a correction collar.

PI: Dr. Diano Marrone

H ITACHI SU-1510 SCANNING ELECTRON MICROSCOPE

The SEM is used primarily to gather topographical or morphological information for a

variety of specimen types. Some sample composition information can also be collected

using the backscattered electron detector. Non-conductive samples can be imaged

quickly and easily in the variable pressure mode, or coated with a sputter coater and

imaged under high vacuum. The 1510 SEM can obtain a resolution of 3 nm under high

vacuum or 4 nm with variable pressure. Samples up to 153 mm in diameter and 60 mm

in height can be imaged using this SEM.

PI: Dr. Mihai Costea


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OTH ER SPECIALIZED EQUIPMEN T

ANY-MAZE ANIMAL BEHAVIOUR VIDEO TRACKING SYSTEM

ANY-maze is a video tracking system designed to automate testing in a wide range of

behavioral apparatus, including various mazes, boxes, tanks, and cages.

PI: Dr. Paul Mallet

BECKMAN LS 6500 SCINTILATION COUNTER

The scintillation counter is used for measuring radioactivity in solid or liquid samples.

Liquid samples can be prepared in either in the standard 20 ml vial or in a 6 ml miniature

vial. The LS6500 can also be used for single photon counting and dual label studies.

PI: Dr. Deb MacLatchy, Dr. Matt Smith

BECKMAN OPTIMA MAX BENCHTOP ULTRACENTRIFUGE

This ultracentrifuge can be used for applications ranging from pelleting to gradient

separations for proteins, DNA, RNA, subcellular fractions, membranes, virus,

lipoproteins, and other sample types. There are currently two rotors available for this

centrifuge: the MLS-50 rotor can accommodate 4 x 5 mL tubes with dimensions of 13 x

51 mm, and can accelerate up to 280,000 x g; the TLA-100.3 rotor can accommodate 6

x 3.5 mL tubes with dimensions of 11 x 32 mm, and can accelerate up to 541,000 x g.

PI: Dr. Matt Smith

GR-202ANALYTICAL BALANCE (210 G X 0.0001G)

A precision scale used for mass measurements of up to 210 g. It has a resolution of 0.01

mg and repeatability of 0.02 mg.

PI: Dr. Paul Mallet

LABCONCO FREEZE DRIER

The freeze drier can be used to lyophilize a variety of sample types and volumes for

shipping or storage. Compared to other types of freezing, lyophilization causes less

damage and allows for the retention of structure and some viability. Samples

containing acids or solvents cannot be freeze dried on this instrument.


LEICA CM3050CRYOSTAT

The Leica CM3050 cryostat is used for cryosectioning delicate samples. The specimen

can be oriented very precisely to produce high quality and reproducible thin serial

sections. Sample temperature can be lowered to -50 oC. This cryostat can accommodate

samples up to 40 x 55 cm in size and sectioning widths can be set from 0.5 to 300 m.

PI: CFI-funded shared equipment located in the Animal Care Facility;contact Dr. Paul

Mallet before using.


15/19


METROHM 633 VOLTAMETRIC ANALYZER

Voltammetric instrumentation for trace to ultra-trace level metal detection and

speciation analysis. The system consists of a dropping mercury electrode (Metrohm

633 VA) and potentiostat unit (Autolab PGSTAT302N).

PI: Dr. Scott Smith

SARTORIUS SE2ULTRAMICROBALANCE

This ultramicrobalance has a capacity of 2.1 g and 0.1 g readability and is equipped

with air buoyancy correction, automatic internal calibration, and draft shield. The data

can be automatically transferred to a spreadsheet to speed weighing of multiple

samples.

PI: Dr. Jim McGeer

SHIMADZU TOC-5050 TOTAL ORGANIC CARBON ANALYZER

The TOC analyzer can be used to measure total dissolved carbon, inorganic carbon,

organic carbon, and non-purgeable organic carbon in water samples. All samples

undergo combustion at 680o C, followed by non-dispersive infrared detection.

Quantitation is based on a calibration curve, and detection ranges from 50 ppb to 4000

ppm (or 5000 ppm for IC).

PI: Dr. Jim McGeer

MISONIX S-4000SONICATOR

This programmable sonicator features a touchscreen controller and sound enclosure.

The output amplitude is controlled from 1-100% giving a greater degree of resolution and

the ability to pinpoint the amplitude needed to effectively process the sample. No manualtuning is required.

BARNSTEAD MEGA-PURE MP-1WATER STILL

This all-glass still delivers ultrapure Type II water at a rate of 1.4 liters per hour. The

vertical condenser design helps ensure high purity water, and the high-temperature cut-

off prevents heating element burn-out.

PI: Dr. Paul Mallet

KAISER REPOKID 5360COPY STAND

This copy stand can be used to photography specimens with minimal shadow on a

none-reflective black background. The camera is supported by a column with a cm/inch

scale, and the specimen is illuminated by two bright bulbs. The black matt base is 32 by

38 cm in size.

PI: Dr. Frederique Guinel


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T RAI N I N G A N D U SE R F E E S

USER TR AIN IN G AN D CER TI F IC ATIO N

All users must receive training before initial use. Training includes a brief discussion

of theoretical background, hazards, and operating principles, followed by hands-on

instrument use. Before training, each user must review the standard operating procedure

for an instrument. Please contact the Instrumentation Technician to arrange a mutually

agreeable time for training. Previously approved users do not need to be retrained. A list

of approved users will be posted near each of the multi-user instruments. A training fee

applies for external users only.

I N T E R N AL USE R F E E S

Internal users are not currently charged for instrument training.

User fees have been implemented to alleviate regular maintenance and consumable

costs for multi-user instruments. The fees listed in Table 1 will be in effect for the 2010-

2011 fiscal year (May 1, 2010 April 30, 2011). These fees will be adjusted as needed

after April 30, 2011 to account for changing consumable costs and increasing

maintenance as the instruments age. Fees for new specialized instruments will beappended on an ongoing basis.

The use of specialized equipment requires permission from the PI, and user fees for

specialized equipment are determined on an individual basis, depending on the

experiment and collaboration.


17/19


Table 1: Internal user instrument fees

INSTRUMENT USER FEE

Steris Prevaccum Sterilizer $ 0.60 / cycle

Miele Glassware Washer $ 0.50 / cycle

Milli-Q Synthesis A-10 $ 0.40 / literCary Eclipse Fluorescence Spectrometer ---

Cary 50 UV-Vis Spectrometer ---

Beckman LS 6500 Scintillation Counter $ 2.15 / sample

Beckman Avanti J30I Centrifuge ---

Centrivap Centrifugal Concentrator ---

Aminco/Thermo French Press ---

Olympus FV1000 Laser Scanning Confocal

Microscope

$ 20.00 / hour

or $1500 / year

Hitachi SR1510 Scanning ElectronMicroscope

$ 35.00 / hour

Acetonitrile $ 1.53 / 25 ml

DMF $ 1.61 / 25 ml

Ethyl Ether $ 1.43 / 25 ml

Glasscontour Dry Solvent system* Hexane $ 1.35 / 25 ml

Methanol $ 1.45 / 25 ml

Methylene Chloride $ 1.67 / 25 ml

THF $ 1.77 / 25 ml

Toluene $ 1.37 / 25 ml

Insta-Ice 475 Dry Ice Block Maker* $ 4.75 / block

*These costs may be covered directly by the principal users.


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E XT E R N AL U SE R F E E S

External users must receive permission from a PI before using any of the specialized

instruments listed in Table 2.

External users must also be trained on any instrument before initial use. Followingtraining, supervision may be required depending on the instrument and whether or not

use is part of a collaboration with a science faculty member at Laurier. Any consumables

required for instrument operation are also charged to the user. The fees listed in Table 2

will be in effect for the 2010-2011 fiscal year (May 1, 2010 April 30, 2011). Fees for

new specialized instruments will be appended on an ongoing basis.

Table 2: Instrument training and user fees for ex ternal users*

INSTRUMENT

TRAINING FEE

(ESTIMATED

TRAINING TIME )

ASSISTED

USE

(PER HOUR)

UNASSISTED

USE

(PER HOUR)

Aviv Circular DichrosimSpectrometer

$ 35 / hour(2-3 hours)

$ 20 $ 10 **

Hitachi SU1510 ScanningElectron Microscope


$ 60 $ 45

Licor DNA Sequencer$ 35 / hour(3-4 hours)

$ 20 $ 10 **

Leica CM3050 Cryostat$ 35 / hour

(1 hour)$ 20

$ 10 per houror $50 per day

Olympus FV1000 ConfocalLaser Scanning Microscope


$ 40 $ 30

* These fees apply to academic users only; please contact the InstrumentationTechnician for commercial user fees.

**An additional fee for consumables may apply


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TE C HN IC AL P R OB LEMS AN D E QUIP MEN T DAMAGE

Technical problems encountered during normal use of a multi-user or specialized

instrument must be reported to the Instrumentation Technician immediately, and recorded

in the log book. All repairs and the regular maintenance described in the SOP for multi-

user instruments will be coordinated or carried out by the Instrumentation Technician.

Users should not attempt to repair any of the multi-user instruments. Depending on

funding, repairs that are required as a result of normal use will be covered by the Faculty

of Science for instruments where the Faculty of Science is listed as the PI. Any damage

that is the result of user misconduct or neglect may be charged to the user.

instrumentation handbook 2010-2011

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