NanosenseProf. dr. ir. Ronald Thoelen

02/12/2014

The project ‘Nanosense’

NANO - SENSOR

READ-OUT TECHNIQUERECEPTOR

Heat Transfer Resistance (HTM)

MIP

Target-molecule

monomer

MAA

cross-linker

EGDM

CHCl3porogene

initiator

AIBN

+

+

Synthetic receptors (MIPs)

Nanosense

1. Heat-Transfer Resistance Based Analysis of Bioparticles (US2014011198)▪ Applicants: IMEC and UHasselt

▪ Status: granted in EP, close to granting in US

▪ Scope: biosensing device for detection and/or characterization of target DNA and/or RNA bioparticles

▪ Current application not covered by this patent

2. Heat-Transfer Resistance Based Analysis of Bioparticles (US2014242605) ▪ Applicants: IMEC and UHasselt

▪ Status: EP-search report available, office action March 2015

▪ Scope: biosensing device for detection and/or characterization of target bioparticles, a.o. cells or small molecules

▪ Current application covered by this patent application

IP

Literature @ UHasselt

Proof-of-concept

The technique has been proven for:

TARGETs: nicotine, serotonin, histamine, caffeïne, malachite green, biogenic amines, …

ANALYTEs: buffer solutions, water, saliva, urine, blood plasma, wholeblood, …

Dose-response curve of

nicotine in PBS

=> LOD ~ 30 nM

HTM-determination

of serotonin in non-diluted blood plasma

Marloes Peeters et al., Analytical and Bioanalytical Chemistry 405, 6479 – 6487 (2013)

Bram Geerets et al., Sensors 13, 9148 – 9159 (2013).

State-of-the-art

- Use of bulk-MIPs

- Stamping technique / spraycaoting

- Flow-cell and drop-cell

- Home-made setup

Valorisation

• What are the most interesting TARGETs? (market potential, emerging technologies, FTO, etc.)

• Detection limits and dynamic range?

• Complexity of the analytes (preparation necessary?)

• Single testing or multi-parameter testing?

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