irmi units biomat
TRANSCRIPT
BIOMATERIALI AD USO BIOMEDICALE BASATI SU
POLISACCARIDI NATURALI (ortopedia, odontoiatria e della chirurgia interna)
Biomat group
1-‐deoxylac+t-‐1-‐yl chitosan: “CHITLAC”
� CHITLAC IS A POLYSACCHARIDE WHICH DERIVES FROM CHITOSAN
OOOH
O
NH2
OO
OHNH
OH
OH
OOH
O
NHOOH
NH2
OH
OH
O
OH
O
OH
OH
OH
OH
O
OH
OH
OH
HH
• SOLUBLE AT PHYSIOLOGICAL pH
• MISCIBLE WITH ANIONIC
POLYSACCHARIDES
• BIOCOMPATIBLE
• BIOACTIVE
lactose
Modificazione chimica per modulare l’attività biologica dei polimeri- materiali BIOATTIVI
es. Chitlac-RGD
Chemical modification of polymers:
conjugation of RGD peptides with
Chitlac
Alginate Polyanion
Gel forming propeties
Chitlac Polycation
Bioactive polymer
Soluble mixtures
ECM-mimic
Bioactivity
Mechanical support
Biopolymer-based scaffold for tissue engineering and cell encapsulation
O
O O
O O
OH
OH O
OH
O
OH O
¯OOC
¯OOC ¯OOC
¯OOC
OH
OH
OH
OH
COO¯ COO¯
COO¯
HO HO
HO HO
HO HO
O
O O
O O
O O
O
HO HO
COO¯
Material preparation and manufacturing and characterization of the final construct
Chemical-physical characterizations • Reology
• NMR • Spectroscopy
Mechanical and structural/ morphological characterizations • SEM • TEM
• Confocal microscopy • Mechanical tests (tensile strength young modulus..)
Biological in vitro testing • Cell viability and proliferation
• Synthesis of ECM components • Differentiation
• Inflammatory response
UNITS experimental activity
Project 1tle
Development of a resorbable sealing patch for the prevention of anastomotic leakage after
colorectal cancer surgical treatment
10
Medical need
10
Colorectal cancer:
• Second most common form of cancer in Europe (2.9 million new cases/year)
• Surgical treatment: resection of the affected bowel and suture of the extremities to restore the normal transit (Anastomosis)
• Most feared complication: ANASTOMOTIC LEAKAGE (up to 23% of cases à mortality 39%)
Tumor
Anastomosis
Surgical removal
COMPLICATION
ANASTOMOTIC LEAKAGE
11
A biopolymeric bioactive patch wrapped around the sutured part of the intestine capable of inducing a faster tissue regeneration, thus
promoting a safe ananstomosis in the critical period of tissue healing
Proposed solu1on
AnastomoSEAL project
Biopolymeric membranes for the treatment of colorectal anastomosis
The AnastomoSEAL Consortium
University of Trieste (Italy) Main role: Coordination, Material development, Clinical Guidance
Impuls (Poland) Main role: Sterilization and
Processing
University of Maastricht (The Netherlands)
Main role: Biological studies (in vivo)
Rescoll (France) Main role: Technological
implementation
FMC-Novamatrix (Norway) Main role: Biopolymer
manufacturing, Guidance towards product
Sigea (Italy) Main role: Development of
innovative bioactive polymers
Biomat lab, DSV
PROMETEO BCTM: OR 1 & 2 CIRI – SDV: OR 2
BIOMAT-UNITS Potential Collaborations