irmi cnr napoli
TRANSCRIPT
• Cripto Loss of Func0on ( Cripto LOF) Guardiola et al., PNAS 2012
• Cripto Gain of Func0on (Cripto GOF)
Modelli Murini condizionali in cellule staminali muscolari
(satelli0)
• OR1-‐Mobilizzazione delle cellule staminali muscolari (satelli0) in modelli murini di danno muscolare acuto
• OR2 -‐ Iden0ficazione di nuovi regolatori delle cellule staminali (Automazione HTS)
IGB “A.B.T.” Gabriella MinchioK
Approcci gene0ci e farmacologici alla rigenerazione muscolare • Proteina Cripto
ricombinante • Delivery sistemico -‐Biopolimeri
Approcci farmacologici
RNA Seq/cellule satelli0
Cripto LOF
96-channel head disposable pipetting tips
The system executes Protocols for targeted differentiation of stem cells Screening of compounds libraries
Advantage/benefits High standard reliability, performance and flexibility Low user intervention Contamination risk reduction
Method standardization Up to 4000 single compuonds simultaneously screened
Complete sterility
Robotic platform: The Cell maker
liquid-handling workstation
laminar air-flow cabinet
medium reservoirs
library MTPs
MTP reader
cell culture MTPs
The CellMaker
+
Cell-based Phenotypic Screening: Identificazione di nuovi regolatori delle
cellule staminali
Cell Proliferation
Cell Colony Phenotype
Targeted Differentiation
• LOPAC • HDAC
Inhibitors
Small molecules
Casalino et al., Journal Molecular Cell Biol, 2011 Casalino et al., Molecular Biotechnology, 2011 Franci et al., under review
Laura Casalino
Eduardo J. Patriarca
Dario De Cesare Sandro De falco
Automation Core
Carolina Prezioso
Ombretta Guardiola Gennaro Andolfi
Collaborazioni
Giorgia Imparato Paolo Netti IIT Napoli