1. SDB? MBC- Molecular Biotechnology Center Biotechnology School University of Torino www.mbcunito.it

2. RESEARCH The main objective is to bring togheter investigators with different scientific backgrounds to facilitate an interdisciplinary approach to biomedical research Basic research Applied research Technology transfer 3. MOLECULAR BIOTECHNOLOGY CENTER Director: Fiorella Altruda 4. STAFF SCIENTISTS: Silvio AIME Full Prof. of Inorganic Chemistry Fiorella ALTRUDA Full Prof. of Molecular Genetics Mara BRANCACCIO Research Scientist of Cell Biology Benedetta BUSSOLATI Associated Prof. of Pharmacology Sara CABODI Research Scientist of Cell Biology Enzo CALAUTTI Research Scientist of Cell Biology Raffaele CALOGERO Associated Prof. of Molecular Biology Giovanni CAMUSSI Full Prof. of Nephrology Enzo CALAUTTI Research Scientist of Cell Biology Federica CAVALLO Associated Prof. of Immunology Paola DEFILIPPI Full Prof. of Cell Biology Ferdinando DI CUNTO Associated Prof. of Molecular Biology Guido FORNI Full Prof. of Immunology Emilio HIRSCH Full Prof. of Molecular Genetics Giorgio MERLO Research Scientist of Cell Biology Valeria POLI Full Prof. of Molecular Biology Paolo PROVERO Research Scientist of Bioinformatics Massimo SANTORO Research Scientific of Molecular Biology Lorenzo SILENGO Full Prof. of Molecular Biology Guido TARONE Full Prof. of Cell Biology Daniela TAVERNA Associated Prof.of Molecular Biology Enzo TERRENO Associated Prof. of Inorganic Chemistry Emanuela TOLOSANO Associated Prof. of Molecular Genetics Emilia TURCO Associated Prof. of Genetics 5. 2013 Researchers (faculty, postdocs, predoctoral fellows, technicians): 204 Average age : 32 26 % man 74 % women Geographic area: 88 % Italian 12 % Foreigners 6. Stem Cells Tumour Immunology Cardiovascular Oncology Inflammation Neurobiology Molecular Imaging Bioinformatics 7. STEM CELLS GPSC generation and differentiation for cell therapy. CARDIOVASCULAR Hypertrophic response of cardiac muscle cells and repair or heart damage in mouse mutants in melusin and PI3K genes. Shape the cardiovascular system and regulate vascular angiogenesis and myogenesis. ONCOLOGY Regulation of proliferation and invasiveness of tumor cells in mouse mutants in p140, p130 and PI3K. Role of cytokines in the immune recognition of tumors and the potential of DNA vaccine to prevent cancer progression. INFLAMMATION Role of the enzyme phosphatidyl-inositol 3-kinase (PI3K) and of the transcription factor StAT3 in leukocyte migration and proliferation during an inflammatory process. Characterization of mechanisms of heme-iron recovery under physiologic and pathologic conditions NEUROBIOLOGY Molecular pathways that control the cross-talk between intracellular membranes and cytoskeleton, in biological processes as different as neuronal differentiation and cytokinesis. 8. MOLECULAR IMAGING Magnetic resonance and optical imaging to visualize differentiation both in the mouse and the zebrafish models, at the cellular level. BIOINFORMATICS A series of new and innovative algorithms capable to integrate the enormous mass of data available in public repositories and data bank have been developed 9. Center for Molecular Imaging Technology Transfer Center for in vivo Functional Genomics 10. FACILITIES: Animal House for mice (12.000) and Rabbits (15) Generation of Transgenic and Knockout mice Molecular Imaging Production and purification of recombinant proteins in Prokaryotes and Eukaryotes Production and characterization of monoclonal and polyclonal antibodies Functional Genomics Zebrafish facility 11. RESEARCH GRANTS FUNDING FROM:European CommunityItalian Association for Cancer Research (AIRC)Telethon for Genetic DiseasesHuman Frontieres Science Project FIRB (Italian Ministry of University and Research)PRIN (Italian Ministry of University and Research)Italian Ministry of Health.RESEARCH CONTRACTS WITH INDUSTRY: BraccoSeronoGlaxo SmithKline FreseniusBiPCaRiboVax Bitechnologies NiCox Research InstituteCureTechIrX TherapueticsAgennix Inc, NanogenBioline 12. EDUCATIONAL In the Biotechnology School we develop the teaching program: Degree in Biotechnology Master in BioHealth Computing Master in Bioinformatics PhD program in Molecular Biotechnology PhD program in Cell Biology 13. The Technology Transfer (TT) Center, also named the hatchery for business ideas, of the MBC provides fully equipped laboratories together with the scientific support knowledge and competence needed to develop applications, investment and business plans. 14. ONCOGENESIS AND TUMOR IMMUNOLOGY B. Bussolati, S. Cabodi, G. Camussi, F. Cavallo, P. Defilippi, G. Forni, V. Poli, F. Retta. Buttiglieri S, Deregibus MC, Bravo S, Cassoni P, Chiarle R, Bussolati B, Camussi G. Role of Pax2 in apoptosis resistance and proinvasive phenotype of Kaposi's sarcoma cells. J Biol Chem. 2004; 279: 4136-43. Bussolati B, Deambrosis I, Russo S, Deregibus MC, Camussi G. Altered angiogenesis and survival in human tumor-derived endothelial cells. FASEB J. 2003; 17: 1159-61. Cabodi S, Tinnirello A, Di Stefano P, Bisaro B, Ambrosino E, Castellano I, Sapino A, Arisio R, Cavallo F, Forni G, Glukhova M, Silengo L, Altruda F, Turco E, Tarone G, Defilippi P. p130Cas as a new regulator of mammary epithelial cell proliferation, survival, and HER2-neu oncogene-dependent breast tumorigenesis. Cancer Res. 2006; 66: 4672-80 Defilippi P, Di Stefano P, Cabodi S. p130Cas: a versatile scaffold in signaling networks. Trends Cell Biol. 2006; 16: 257-63 Nanni P, Nicoletti G, De Giovanni C, Landuzzi L, Di Carlo E, Cavallo F, Pupa SM, Rossi I, Colombo MP, Ricci C, Astolfi A, Musiani P, Forni G, Lollini PL. Combined allogeneic tumor cell vaccination and systemic interleukin 12 prevents mammary carcinogenesis in HER-2/neu transgenic mice. J Exp Med. 2001; 194: 1195-205. Lollini PL, Cavallo F, Nanni P, Forni G. Vaccines for tumour prevention. Nat Rev Cancer. 2006; 6: 204-16. 15. CARDIAC HYPERTROPHY IN HEART FAILURE L. Barberis, M. Brancaccio, E. Hirsch and G. Tarone Brancaccio M, Fratta L, Notte A, Hirsch E, Poulet R, Guazzone S, De Acetis M, Vecchione C, Marino G, Altruda F, Silengo L, Tarone G, Lembo G. Melusin, a muscle-specific integrin beta1-interacting protein, is required to prevent cardiac failure in response to chronic pressure overload. Nat Med. 2003;9: 68-75 Crackower MA, Oudit GY, Kozieradzki I, Sarao R, Sun H, Sasaki T, Hirsch E, Suzuki A, Shioi T, Irie-Sasaki J, Sah R, Cheng HY, Rybin VO, Lembo G, Fratta L, Oliveira-dos-Santos AJ, Benovic JL, Kahn CR, Izumo S, Steinberg SF, Wymann MP, Backx PH, Penninger JM. Regulation of myocardial contractility and cell size by distinct PI3K-PTEN signaling pathways. Cell. 2002; 110: 737-49. Patrucco E, Notte A, Barberis L, Selvetella G, Maffei A, Brancaccio M, Marengo S, Russo G, Azzolino O, Rybalkin SD, Silengo L, Altruda F, Wetzker R, Wymann MP, Lembo G, Hirsch E. PI3Kgamma modulates the cardiac response to chronic pressure overload by distinct kinase-dependent and -independent effects. Cell. 2004; 118: 375-87. 16. STEM CELLS B. Bussolati, E. Calautti, G. Camussi, and F. Di Cunto Herrera MB, Bruno S, Buttiglieri S, Tetta C, Gatti S, Deregibus MC, Bussolati B, Camussi G. Isolation and characterization of a stem cell population from adult human liver. Stem Cells. 2006 Aug 31 Bussolati B, Bruno S, Grange C, Buttiglieri S, Deregibus MC, Cantino D, Camussi G. Isolation of renal progenitor cells from adult human kidney. Am J Pathol. 2005; 166: 545-55. Calautti E, Li J, Saoncella S, Brissette JL, Goetinck PF. Phosphoinositide 3-kinase signaling to Akt promotes keratinocyte differentiation versus death. J Biol Chem. 2005; 280: 32856-65. Camera P, da Silva JS, Griffiths G, Giuffrida MG, Ferrara L, Schubert V, Imarisio S, Silengo L, Dotti CG, Di Cunto F. Citron-N is a neuronal Rho-associated protein involved in Golgi organization through actin cytoskeleton regulation. Nat Cell Biol. 2003; 5: 1071-8. 17. BIOINFORMATICS F. Di Cunto, P. Provero and L. Silengo Pellegrino M, Provero P, Silengo L, Di Cunto F. CLOE: identification of putative functional relationships among genes by comparison of expression profiles between two species. BMC Bioinformatics. 2004; 5: 179. Cora D, Herrmann C, Dieterich C, Di Cunto F, Provero P, Caselle M. Ab initio identification of putative human transcription factor binding sites by comparative genomics. BMC Bioinformatics. 2005 May 2;6:110. 18. SDB? 19. MBC- Molecular Biotechnology Center FACILITIES: Animal House for mice (15.000) and rabbits (15) Generation of Transgenic and Knockout mice Production and purification of recombinant proteins in Prokaryotes and Eukaryotes Production and characterization of monoclonal and polyclonal antibodies 6 main scientific area of interest: Inflammatory diseases Oncogenesis and tumor immunology Cardiac hypertrophy in heart failure Stem cells Coupled with Interfaculty Biotechnology School More than 200 scientists Neurobiology Bioinformatics Molecular Imaging