PTR-QiTOF analysis and applications

Jurriaan Mes

Wageningen - Food & Biobased Research

Food & Health Research

Application

Aroma’s released from products, in mouth and in nose cavity

Ex-healed breath analysis

Aroma’s

Fast, on line Effect of chewing Interaction with saliva/enz Released in cavity

Exhaled breath analysis, and others

Adapted from Metabolites 2015, 5(1), 3-55;

Some common biomarkers

Adapted from Metabolites 2015, 5(1), 3-55;

Liver disease

Diagnose chronic liver disease (CLD) patients, compensated cirrhosis (CIR) from those without cirrhosis

Scientific Reports 6, Article number: 19903 (2016)

Nr. Chemical identity Change

1 3-methylbutanal (−)

2 Propanoic acid (+)

3 Octane (+)

4 Terpene (C10H16) (+)

5 Terpenoid: α-pinene (+)

6 3-carene (+)

7 Unknown (+)

8 Branched C16H34 (+)

9 1-hexadecanol (−)

10 Branched C16H34 (−)

11 Dimethyl disulfide (+)

IBD children

Aliment Pharmacol Ther. 2014 Sep; 40(5): 498–507.

What we did

Developed protocol● Effect of pre-digested food/ beverages● How long and how often to breath in tube● Correct background VOC

SenTo Panel breath marker development

T2D Asthma Gut complains

Can we correlate VOC from exhaled breath with health status● Which biomarkers can be analysed direct in breath● How strong /predictive are biomarkers● How specific are biomarker for health status

● Single marker or based on model ● Combine with other (blood based) biomarker

Breath analysis update

Medical Ethical Approval received● Had to show the accuracy and sensitivity of the PTR-QiTOF ourselves

Recruitment and analyse Target 25 persons in each group Analyse breath 1x per month, 4x months Questionnaire on health at same time (variable over

time) Now evaluating the data Next

● Continue with same population

● Include also another health issue

Problems and opportunities

Problems Data analysis from raw to

processed

● Need to be more automated

Need support from processed to biomarker

● Statistics, biometris

Opportunities Quick diagnose of diseases,

inflammation, cancers etcfor hospitals

Easy assessments of health status and effect of an (food) intervention/ treatment

Promising direction for sensors when validated biomarkers and models for translation have been developed

Thank you for the attention

Jurriaan.mes@wur.nl

Taste and ex-healed breath VOCs

ReleasedTaste and ex-healed breath VOCs