Life sciences

HTRF® Kits & reagents

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For 25 years, Cisbio Bioassays has been committed to

offering innovative solutions and kits to speed up research

into new medicines.

From oncology and CNS to metabolic disorders,

rare diseases, and more, Cisbio helps you reach your drug discovery objectives with trusted solutions that streamline your research

efforts

With its headquarters in France and 3 subsidiaries around the world, Cisbio has never been closer to you, working with your

team in a true partnership day after day.

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Click on the interested product and be redirected

to the associated product on Cisbio website.

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SUMMARY

GPCRs

Cell signaling Phospho-assays

Cell signaling Cytokines

Biomarkers

Biotherapeutic development tools

Cisbio drug discovery services

Biochemical & cell-based epigenetics

KinEASE™

Protein-protein interaction tools

HTRF® compatible readers

Therapeutic areas

Kits and reagents to speed up your projects

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GPCRs

cAMP IP1

p-ERKp-AKT

Tag-lite®

Cell-based kits for GPCR investigation

• Second messengers (cAMP, IP-One)

• Ready-to-use binding assays: Tag-lite®

• Downstream phospho-markers: p-AKT, p-ERK

www.cisbio.com/gpcr

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Explore the cell function

• Up to 20 times more sensitive than WB

• Validated on 2D and 3D cell cultures, cell tissues and tumor xenografts

• High species cross-reactivity

• A single, simple add-and-read protocol for all kits

* Phospho & Total

Listen toyour cellular

pathway

Cell signaling

Phospho-assays

p-VASP*

p-LRRK2*p-Tau*

p-SMAD1*p-SMAD3

p-IKKβp-NFκB

p-ACCp-AMPK*

p-AKT* p-mTor

p-STAT3* p-STAT5

p-p38MAPK p-JNK p-cJun

p-YAP

p-H2AXp-Histone H3*

p-β-catenin* p-GSK3 α* p-GSK3 β*

Advanced pERK p-ERK* p-MEK1

p-MEK1/2* P-CREB

p-EGFR* p-HER2* p-HER3*

p-IR beta*p-VEGFR2

p-S6RP p-EIF4E p-70S6K p4-EBP1

p-Badp-BTK

www.cisbio.com/cell-signaling

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Cell-based assays made simple

• Strong correlation with ELISA

• Single protocol for all kits

• Low sample consumption: 10 µL

• Easily combined with other detection types (HCS, phospho, label-free)

CCL20 (h)

G-CSF (h)

GROα (h) CXCL1

IFNα (h)

IFNγ (h & m)

IL1α (h & m)

IL1β (h & m)

IL2 (h & m)

IL4 (h)

IL5 (h)

IL6 (h & m)

IL8 (h)

IL10 (h)

IL12 (h)

IL17 (h & m)

IL18 (h)

IL23 (h)

IL31 (h)

MCP1 (h)

MIP-1 β (h)

TNFα (CyPV)

TNFα (h & m)

Cell signaling

Cytokines

www.cisbio.com/cytokines

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From ready-to-use kits to custom assay development:

• A cost-effective alternative to ELISA

• Simple add-and-read protocol

• Reproducible, highly sensitive assays

Histamine

LTB4 / LTC4Prostaglandin E2

Amyloid beta 1-40 (h)alpha-SynucleinAPP substrate

Biotin-APP substrate-KGAD67

MANF (h)MAP-Tau

Tau aggregation

Active GLP1Glucagon

IGF1 (r)Insulin

Apo A1/B/C3/E (h)

c-Myc cell-based (h)

FMRP (h)Frataxin (h)MBNL1 (h)

SMN (h)Utrophin (h & m)

AldosteroneCortisolEstradiol

ProgesteroneTestosterone

cGMP

Heme Oxygenase (h)PCSK-9 (h)Pro-collagen Type 1 (h)cleaved PARP

alpha Tubulin acetyl K40alpha Tubulin

Biomarkers

www.cisbio.com/biomarkers

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Tools for biotherapeutic characterization

• CD16 and variant assays for ADCC prediction

• Human and mouse IgG quantification kits

• CHO host cell protein kit for contaminant quantification

• Tag-lite® for Ab screening on cell surface targets

• Cell-signaling assays: cytokines, P-proteins, 2nd messengers

Biotherapeutic

development tools

www.cisbio.com/biotherapeutics

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Fast, reliable services provided by HTRF experts

• Protein/antibody labeling in 3 days

• Antibody pair for immunoassays in 2 weeks

• Full HTRF assay development

• Early ADCC prediction for MAb optimization

• Compound profiling

Cisbio

drug discovery

services

www.cisbio.com/htrf-services

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Explore epigenetic modifications

• Specific cell-based assays

• Specific and universal biochemical assays

• Extensive enzyme validation

• Downstream signaling assays

Binding domain assays

Universal SAH detection formethyltransferases

Epigenetic cell-basedmethyltransferase

assay

Epigenetic markspecific assays

Biochemical

&

cell-based

epigenetics

www.cisbio.com/epigenetics

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Kits for Serine/Threonine and Tyrosine kinases

• Over 107 Ser/Thr and 58 Tyr kinases validated

• Complete kit for simplified kinase assay development

• Compatible with a broad range of [ATP]

• Low enzyme comsumption

KinEASE™

www.cisbio.com/kinease

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Benefit from unlimited flexibility for your assay development

• Validated on numerous target configurations

• Anti-tag: 6His, c-myc, FLAG, GST, MBP, HA, DNP

• Anti-species: human, rabbit, mouse, prot A

• Affinity: streptavidin

• Custom labeling services for coupling untagged proteins

Protein-protein

interaction tools

www.cisbio.com/toolbox

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HTRF® compatible

readers

www.cisbio.com/htrf-compatible-readers

More than 30 validated readers across 7 brands

• High standards for reader certification to serve you more effectively

• Freedom to choose equipment that matches your exact needs

• Your guarantee for top quality HTRF readouts

Can’t find your microplate reader? Check its compatibility by contacting your local Cisbio Bioassays sales and application support team.

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HTRF Solutions for Therapeutic Areas

From oncology and CNS to metabolic disorders, rare diseases, and more, Cisbio helps you reach your drug discovery objectives with trusted solutions that streamline your research efforts.

Therapeutic areas

Oncology Infectious diseases

Inflammation / Immunology

Rare diseases

Neurosciences

Metabolism / Diabetes

Cardiovascular

www.cisbio.com/therapeutic-areas

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* Homogeneous Time-Resolved Fluorescence

Europe and other countries +33(0)466 796 705

bioassays@cisbio.com

U.S. and Canada 1-888-963-4567

htrfinfo@cisbio.us

China +86 21 5018 9880

htrfinfoasia@cisbio.cn

Japan +81-(0)43-306-812

htrf@cisbio.jp

Kits and reagents

to speed up

your projects

www.cisbio.com