lycopene extraction from tomatoes ana Álvarez martÍn laura hernandez salamanques
TRANSCRIPT
LYCOPENE EXTRACTION FROM TOMATOES
ANA ÁLVAREZ MARTÍN LAURA HERNANDEZ SALAMANQUES
CONTENT
• Introduction• Extraction using a solvent• Supercritical extraction• Extraction assisted by enzyme
LYCOPENE
• Type of carotenoid• Aditive, colorant and
antioxidant• Tomato pomace• Ripe tomatoes
CONTENT
• Introduction• Extraction using a solvent• Supercritical extraction• Extraction assisted by enzyme
EXTRACTION USING A SOLVENT
• Fat-soluble: extracted with organic solvents• Raw material: tomato waste skin• Solvent:
hexane:acetone:alcohol (2:1:1) + BHT.
• Variables
EXTRACTION USING A SOLVENT VARIABLES
Number of extractionSolvent/meal ratio
Solvent/meal ratioParticle size
EXTRACTION USING A SOLVENT VARIABLES
TemperatureNumber of extractions
Particle sizeNumber of extractions
EXTRACTION USING A SOLVENT VARIABLES
Extraction timeNumber of extractions
EXTRACTION USING A SOLVENT OPTIMUS CONDITIONS
solvent/meal: 30:1 v/w4 extractions50 °Cparticle size 0.15 mmextraction time 8 min. Lycopene yield: 1.99 mg/100 g
CONTENT
• Introduction• Extraction using a solvent• Supercritical extraction• Extraction assisted by enzyme
SUPERCRITICAL
• Supercritical fluid extraction (SFE)– Tc, Pc solvent– CO2: • low critical temperature, • not toxic, • not flammable, • low cost ,• high purity
SUPERCRITICAL
• Semi-continuos process• Equipment: an extractor, a back pressure
regulator, a chiller, a high pump for CO2, collection vials and a wet gas meter.
• Optimization: tomato seed oil (co-solvent)
SUPERCRITICAL
Constant flow rate:
SUPERCRITICAL
Constant pressure:
SUPERCRITICAL
Constant temperature:
SUPERCRITICAL
• OTHER FACTORS:– Rising method– Tomato peel to seed ratio– Particle size– Degradation lycopene
CONTENT
• Introduction• Extraction using a solvent• Supercritical extraction• Extraction assisted by enzyme
EXTRACTION ASSISTED BY ENZYME
• Improve lycopene extraction• Response surface methodology (RSM)• Chemicals:– Acetona, ethanol, hexane– BHT
• Enzimes:– Peclyve PR– Cellulyve 50LC
EXTRACTION ASSISTED BY ENZYME
• Optimal conditions:– T=30ºC– Extraction time=3.18h– Enzyme load=0.16kg/kg– Enzyme preparation= 50:50 Pec-Cel