manuchar gvaramia ,
DESCRIPTION
Study of Influence of Salts Additives to M ixed Reverse Micelles via Reverse Microemulsion Chromatography and UV-visible spectroscopy. Manuchar Gvaramia , Postgraduate student , Department of Chemistry, Faculty of Exact and Natural Sciences, Tbilisi University, Georgia. Reverse micelle. - PowerPoint PPT PresentationTRANSCRIPT
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Study of Influence of Salts Additives to Mixed Reverse Micelles via Reverse
Microemulsion Chromatography and UV-visible spectroscopy
Manuchar Gvaramia,
Postgraduate student, Department of Chemistry, Faculty of Exact and Natural Sciences,
Tbilisi University, Georgia
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Reverse micelle Direct micelle
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Mixed microemulsions on the basis of nonionic and anionic surfactants
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Dependence of retention factors of solutes on the water/surfactant ratio
0
1
2
3
4
5
6
7
8
0 1 2 3 4 5 6
Water/surfactant ratio
Re
ten
tio
n f
ac
tor,
k
Caff
PB
CBZ
HQ
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Dependence of retention factors of solutes on the water/surfactant ratio
0
1
2
3
4
5
6
0 1 2 3 4 5 6
Water/surfactant ratio
Ret
enti
on
fac
tor,
k
RC
PL
NB
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Water,Brij-30, butanol and hexane with ratio (v/v.%): 0.3/2.8/4.6/92.3
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Water,Brij-30, butanol and hexane with ratio (v/v.%): 0.7/2.8/4.6/91.9
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Diagram of retention factors of solutes at w=2( ) and w=3 ( ) in the presence of sodium acetate(0.025M)
0
2
4
6
8
HQ PB CBZ Caff
Ret
enti
on
fac
tor,
k
w=2
w=3
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Diagram of retention factors of solutes at w=2( ) and w=3 ( ) in the presence of sodium acetate(0.025M)
0
2
4
6
8
NB RC PL
Ret
entio
n fa
ctor
, k
w=2
w=3
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Diagram of retention factors of solutes at w=2() and w=3() in the presence of potassium perchlotate(0.025)
0
2
4
6
8
HQ PB CBZ Caff
Ret
enti
on
fac
tor,
k
w=2
w=3
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Diagram of retention factors of solutes at w=2() and w=3() in the presence of potassium perchlotate(0.025)
0
2
4
6
8
NB RC PL
Ret
enti
on
fac
tor,
k
w=2
w=3
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Diagram of retention factors of solutes at the presense of sodium acetate () and potassium perchlorate() W=2
0
2
4
6
8
RC PL CBZ Caff PB
Ret
entio
n fa
cto
r, k
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O-nitroaniline as molecular probe
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Determination of binding constants of o-
nitroaniline (o-NA) to Brij-30 reverse micelles
0]30[][
][
BrijNAo
NAoK
f
bb
398398398
376376376
ANAoNAo
ANAoNAo
bbff
bbff
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Absorption spectra of o-NA in hexane/(♦-0.0M Brij-30+ 0.1M AOT, 0.13 ·10-2M Brij-30+ 0.1AOT, 0.91·10-2M Brij-30+ 0.1M AOT, 2.73·10-2M Brij-30+ 0.1M AOT,
11.7·10-2M Brij-30+0.1M AOT, 23.4·10-2M Brij-30+ 0.1M AOT) at Brij-30 concentrations. At W0=0, [o-NA]=4.5·10-5M
0
0,02
0,04
0,06
0,08
0,1
0,12
0,14
0,16
0,18
300 350 400 450 500
Wavelength/nm
Ab
sorb
ance 0
0.13
0.91
2.73
11.7
23.4
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Variation of λm of o-NA in reverse micelles as a function of Brij-30 concentration
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Diagram of Kb of o-NA with (Brij-30+AOT) mixed micelles in the following conditions:1) absence of water, 2) presence of
water, 3) presence of 0.05M sodium acetate and 4) 0.05M
potassium perchlorate
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a) Diagram of retention factors of solutes in the presence of sodium acetate (■) and potassium perchlorate (□) W=2
b) Diagram of binding constants of o-NA with (Brij-30+AOT) mixed micelles in the presence of 0.05M sodium acetate(■) and 0.05M potassium perchlorate(□)
0
50
100
Kb, M
-1
ab
0
2
4
6
8
RC PL CBZ Caff PB
Ret
entio
n fa
ctor
, k
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Conclusions
• Binding of o-nitroaniline to reverse micelles is higher in the presence of chaotropic perchlorate ions in comparison with kosmotropic acetate ions.
• Solubility of the model compounds is lower in the presence of chaotropic anions in the water pools of reverse micelles as compared with kosmotropic acetate ions.
• Retention factor of the solutes are higher in the presence of kosmotropic acetate ions in the water nanocages of the reverse micelles as compared to perchlorate ions.
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Conclusions
• Results may be useful in the investigations of water structure, when it is confined to nanometer-scale cavities, viz. in biological systems.
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Acknowlegement
• I thank the Georgian Shota Rustaveli National Science Foundation and Julich Scientific Center for opportunity to participate in this project
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Thanks for attention
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• SO42- > F- > OAc- > Cl- > Br- > NO3
- > I- >
ClO4- > SCN-
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Benzene-1,3-diolresorcinol benzene-1,2,3-triol
pyrogallol
benzene-1,4-diolhydroquinone
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1,3-dimethyl-7H-purine-2,6-dionetheophylline
1,3,7-trimethyl-1H-purine-2,6(3H,7H)-dionecaffeine
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2,2,2-trichloroethane-1,1-diol
Phenobarbital (5-ethyl-5-phenylbarbituric acid or 5-ethyl-5-phenylhexahydropyrimindin-2,4,6-trione)
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carbamazepine
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Absorption spectra of o-NA in different solvents:1) water (412), 2) ethanol (406 nm), 3) methanol
(405nm) and 4)isopropanol (407 nm)
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Possible scheme of arrangement of ions in water pools of the reverse
micelle