Study of Influence of Salts Additives to Mixed Reverse Micelles via Reverse

Microemulsion Chromatography and UV-visible spectroscopy

Manuchar Gvaramia,

Postgraduate student, Department of Chemistry, Faculty of Exact and Natural Sciences,

Tbilisi University, Georgia

Reverse micelle Direct micelle

Mixed microemulsions on the basis of nonionic and anionic surfactants

Dependence of retention factors of solutes on the water/surfactant ratio

0

1

2

3

4

5

6

7

8

0 1 2 3 4 5 6

Water/surfactant ratio

Re

ten

tio

n f

ac

tor,

k

Caff

PB

CBZ

HQ

Dependence of retention factors of solutes on the water/surfactant ratio

0

1

2

3

4

5

6

0 1 2 3 4 5 6

Water/surfactant ratio

Ret

enti

on

fac

tor,

k

RC

PL

NB

Water,Brij-30, butanol and hexane with ratio (v/v.%): 0.3/2.8/4.6/92.3

Water,Brij-30, butanol and hexane with ratio (v/v.%): 0.7/2.8/4.6/91.9

Diagram of retention factors of solutes at w=2( ) and w=3 ( ) in the presence of sodium acetate(0.025M)

0

2

4

6

8

HQ PB CBZ Caff

Ret

enti

on

fac

tor,

k

w=2

w=3

Diagram of retention factors of solutes at w=2( ) and w=3 ( ) in the presence of sodium acetate(0.025M)

0

2

4

6

8

NB RC PL

Ret

entio

n fa

ctor

, k

w=2

w=3

Diagram of retention factors of solutes at w=2() and w=3() in the presence of potassium perchlotate(0.025)

0

2

4

6

8

HQ PB CBZ Caff

Ret

enti

on

fac

tor,

k

w=2

w=3

Diagram of retention factors of solutes at w=2() and w=3() in the presence of potassium perchlotate(0.025)

0

2

4

6

8

NB RC PL

Ret

enti

on

fac

tor,

k

w=2

w=3

Diagram of retention factors of solutes at the presense of sodium acetate () and potassium perchlorate() W=2

0

2

4

6

8

RC PL CBZ Caff PB

Ret

entio

n fa

cto

r, k

O-nitroaniline as molecular probe

Determination of binding constants of o-

nitroaniline (o-NA) to Brij-30 reverse micelles

0]30[][

][

BrijNAo

NAoK

f

bb

398398398

376376376

ANAoNAo

ANAoNAo

bbff

bbff

Absorption spectra of o-NA in hexane/(♦-0.0M Brij-30+ 0.1M AOT, 0.13 ·10-2M Brij-30+ 0.1AOT, 0.91·10-2M Brij-30+ 0.1M AOT, 2.73·10-2M Brij-30+ 0.1M AOT,

11.7·10-2M Brij-30+0.1M AOT, 23.4·10-2M Brij-30+ 0.1M AOT) at Brij-30 concentrations. At W0=0, [o-NA]=4.5·10-5M

0

0,02

0,04

0,06

0,08

0,1

0,12

0,14

0,16

0,18

300 350 400 450 500

Wavelength/nm

Ab

sorb

ance 0

0.13

0.91

2.73

11.7

23.4

Variation of λm of o-NA in reverse micelles as a function of Brij-30 concentration

Diagram of Kb of o-NA with (Brij-30+AOT) mixed micelles in the following conditions:1) absence of water, 2) presence of

water, 3) presence of 0.05M sodium acetate and 4) 0.05M

potassium perchlorate

a) Diagram of retention factors of solutes in the presence of sodium acetate (■) and potassium perchlorate (□) W=2

b) Diagram of binding constants of o-NA with (Brij-30+AOT) mixed micelles in the presence of 0.05M sodium acetate(■) and 0.05M potassium perchlorate(□)

0

50

100

Kb, M

-1

ab

0

2

4

6

8

RC PL CBZ Caff PB

Ret

entio

n fa

ctor

, k

Conclusions

• Binding of o-nitroaniline to reverse micelles is higher in the presence of chaotropic perchlorate ions in comparison with kosmotropic acetate ions.

• Solubility of the model compounds is lower in the presence of chaotropic anions in the water pools of reverse micelles as compared with kosmotropic acetate ions.

• Retention factor of the solutes are higher in the presence of kosmotropic acetate ions in the water nanocages of the reverse micelles as compared to perchlorate ions.

Conclusions

• Results may be useful in the investigations of water structure, when it is confined to nanometer-scale cavities, viz. in biological systems.

Acknowlegement

• I thank the Georgian Shota Rustaveli National Science Foundation and Julich Scientific Center for opportunity to participate in this project

Thanks for attention

• SO42- > F- > OAc- > Cl- > Br- > NO3

- > I- >

ClO4- > SCN-

Benzene-1,3-diolresorcinol benzene-1,2,3-triol

pyrogallol

benzene-1,4-diolhydroquinone

1,3-dimethyl-7H-purine-2,6-dionetheophylline

1,3,7-trimethyl-1H-purine-2,6(3H,7H)-dionecaffeine

2,2,2-trichloroethane-1,1-diol

Phenobarbital (5-ethyl-5-phenylbarbituric acid or 5-ethyl-5-phenylhexahydropyrimindin-2,4,6-trione)

carbamazepine

Absorption spectra of o-NA in different solvents:1) water (412), 2) ethanol (406 nm), 3) methanol

(405nm) and 4)isopropanol (407 nm)

Possible scheme of arrangement of ions in water pools of the reverse

micelle