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MF-11 Eleventh Microfluidics Consortium

MF11.2 Webinars

Paris, France April 22

Final Agenda V17

Member Profiles

Zoom Sign in Arrangements

During the Covid19 Pandemic we are re-inventing MF11 as a virtual

service – delivering consortium meetings over Zoom

These video meetings are designed to replace most of the content /

processes of MF11.2. Some sessions are Members only, others are

open to registered non-members. We are experimenting with ‘Member

Booths’ for private conversations/demos.

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The Eleventh Microfluidics Consortium brings together current and future stakeholders from across a wide range of backgrounds with a shared interest in facilitating the growth of the microfluidics industry through better understanding of the challenges, opportunities and choices which it faces.

Our current membership includes: BD Biosciences (USA), IPGG (F) ;EV Group (A) Philips (NL); Micronit (NL); Danaher (USA); Dolomite (UK) ; Fluigent (F); Idexx (USA), MicroCAPS (CH), National Research Council of Canada (CAN), Labcyte (USA) ; Lonza (CH) ; IMT (CH), Posalux (CH); Sartorius (USA), Susos (CH), Web Industries (USA); Cryopak (USA), z-microsystems (A) and University of Cambridge (UK).

We organize closed meetings for our members on both sides of the Atlantic where we seek to promote our mission “to grow the market for microfluidics enabled products and services” by

- Finding shared interest across the landscape of applications - Championing modularity and standards where appropriate - Engaging with key industry influencers

While helping our members to do deals along the way.

On this occasion we are delighted to be hosted by IPGG and supported by Fluigent. Our special thanks to Remi Wache and Arnaud Cartier

Pierre-Gilles de Gennes Institute (IPGG) Microfluidics or the science of manipulation of small fluid volumes with new technologies.

Talking of the Industry world and Research, Pierre-Gilles de Gennes used to say that « both have

everything to gain by working together ». Regarding the frontiers between scientific domains, he

stressed that we should be able to overcome them in order to make the domains more successful and

fully exploit their potentials. Pierre-Gilles de Gennes Institute – IPGG – has been created along these

lines of thought: its goal is to bring together, in a cross-disciplinary domain (microfluidics), experts from

various disciplines (physics, biology, chemistry, technology), and develop both fundamental and applied

research. Industrial domains interested by microfluidics are numerous (food industry, medicinal field,

cosmetics, oil industry, instrumentation, ...).

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Zoom meeting arrangements:

We will have several virtual rooms:

- Main Auditorium open to: o Members – who will have full audio/video/screen-share/chat/see other

participants rights o Non-Members - who will be able to see/hear the proceedings and

have chat rights to pose questions thro the meeting host - but will be on mute.

- Members Executive Lounge Open to members only and with full A/V meeting facilities for confidential consortium sessions.

- Members’ Private Booths. Hosted by individual members who can invite any delegates to join them at any time for discussions and shared presentation materials. We are expecting booths hosted by:

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- - See logistics section below for details of how to join this Zoom meeting and

visit the private booths.

April 22 All times are Paris Time = 9 hours ahead of PDT.

13:00 (04:00 PDT) Location: Main Auditorium

Introduction and Welcome

Peter Hewkin - virtual meeting host - CfBI

Remi Wache - physical meeting host - IPGG

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Session 1: “What’s Up?” Members presentations . This session is an

opportunity for members to present on their recent work and achievements.

Each talk will be followed by Q&A. Please ‘raise your hand’ if you want to speak

– the host will unmute your microphone

Philips - Robin de Bruijn “Guidelines for MEMs Manufacture in Silicon”

Philips Innovations Services, segment MMD consists of

two facilities in Eindhoven (The Netherlands)

Micro Devices facility: For prototyping as well as volume

production. Our services are industrial PCBA prototyping,

assembly of high-end PCBAs, interconnect architecture &

prototyping.

MEMS Foundry, High Tech Campus: Our MEMS Foundry

is specialized in low to medium volume custom MEMS

manufacturing of 4, 6, 8 inch and even square substrates.

We offer the flexibility to use substrate materials like silicon, SOI, GaAs, glass, quartz

and CMOS-wafers

IMT – Alexios Tzannis “Structured Functionalization of Surfaces “

IMT AG is a provider of leading edge BioMEMS technology

transferring the microfabrication know-how originally

developed for microelectronics into flexible and scalable

solutions for the manufacturing of micro- and nanostructures

in glass for consumables in life science applications and

components in medical instruments and equipment.

With more than 50 years of experience in the fields of optics and metrology, IMT AG is well positioned in the intersection of microfluidic and photonics to act as a development partner, as well as a large-scale manufacturer of glass consumables for microfluidic and bio photonic applications

Micronit – Mark Olde Riekerink

Micronit is a world leader in developing and manufacturing

microfluidic Lab-on-a-Chip and MEMS products for life

science, medical, and industrial applications. Micronit has

extensive know-how and intellectual property in the field of

microfabrication and microfluidics, combining a start-of-the-

art cleanroom with an experienced product development

and R&D team.

Micronit's company strategy is to keep expanding its

knowledge and strengthening its position on various market

segments, among which In-vitro Diagnostics, including Point-of-Care testing, Pharma, Life

Science Research, Organ-on-Chip and bioMEMS. This strategy is underscored by

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Micronit's continued R&D activities in defining new manufacturing processes, novel

fabrication materials and next generation microfluidics components as well as functional

integration strategies to provide novel, microfluidic formats that enable automation of

laboratory workflows

Richard Gray Dolomite : ParticleGEN Public Domain Presentation

Richard is an executive director of Blacktrace holdings –

with responsibility for Dolomite Microfluidics in the USA.

He will describe the ParticleGEN project which is the

exciting co-development consortium spinning off from

MF11. Enabled by microfluidics technology it will deliver

precision designer coated particles at ton-a-month – for

therapeutic, cosmetic, agricultural and even civil

engineering applications - on a shared cost, shared risk

basis.

14:30 close / break

Members and non-members free to chat by text in the main auditorium and visit the

member booths. If you want to be allocated to a particular member booth chat with

Alice.

14:45 (05:45 PDT) Location: Members Executive Lounge. MF 11 Members will

automatically be invited to the Executive Lounge. They can return to the main auditorium when

they wish by pressing the ‘leave breakout room button’

Session 2 “Discussion”: Addressing big questions put forward by members.

Several members are involved in Covid-19 initiatives – and in the coming year when pressure on

delivering these reduces, have offered to present on their successes and insights to the consortium.

In this session we will seek to identify headline issues which affect us as a consortium..

Q1 Covid19 – Opportunities and Challenges arising for MF11 members.

Q2 Delivering Biochemistry Innovation under lockdown.

Q3 Sustaining innovation thro an economic ice-age.

Other questions as proposed by members.

Hosted by Peter Hewkin

15:15 close

Members and non-members free to chat by text in the main auditorium and visit the

member booths. If you want to be allocated to a particular member booth chat with

Alice.

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15:30 (06:30 PDT) Location: Main Auditorium

Session 3 “IPGG Research”

We will hear from leading microfluidics researchers within the IPGG/Carnot initiative.

Each talk will be followed by Q&A moderated by Remi Wache. Please ‘raise your

hand’ if you want to speak – the host will unmute your microphone

Jacques Fattacciolli - member of the department Microfluidics Laboratory Division of Chemistry, ENS (CNRS UMR 8640)

Oil-in-water emulsion droplets are deformable particles, with a surface that can be functionalized by biologically adhesive molecules. Having a fluid interface, these molecules are laterally mobile, making the whole microparticle able to interact with immune cells and respond to receptor sollicitations.

In this talk, I will present how using emulsion droplets in this context, in addition to microlfuidic devices, is a powerful tool to study phagocytosis, cell migration, and antigen extraction by B cells

Simon Dumas

Simon Dumas is a 3th year PhD student in Stéphanie Descroix laboratory at IPGG and Institut Curie. His work is to develop new droplet microfluidics solutions for single-cell analysis. He has developed a microfluidic device embedding microfabricated magnets to extract magnetic beads from sub-nanoliter droplets as a tool for multimodal single-cell analysis.

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Lucie Barbier

Lucie is a 4th-year doctoral student in Matthieu Piel's laboratory under the supervision of Pablo Vargas at IPGG and Institut Curie. She studies the mechanisms by which immune cells integrate chemical and physical signals from their environment to migrate into tissues. She has developed microfluidic tools to identify cellular components that modulate the migration of immune cells in complex geometries. Her work aims to understand how white blood cell motility could be manipulated to strengthen the immune system in case of infection or to cure immune disorders.

Patrick Tabeling. Directeur de Recherche CNRS, Professor at ESPCI, and former director of the Institut Pierre-Gilles de Gennes

Pr. Patrick Tabeling is leader of the group MMN, a

prominent team in the field of microfluidics. He is the

cofounder of MicroFactory. Visiting researcher in

Chicago University (1984-1985), Directeur de

Recherches CNRS at ENS (1985-2001), visiting

professor to UCLA, Directeur de Recherches/Professor

at ESPCI. He is cofounder of the Institut Pierre-Gilles de

Gennes, and has been its director for the period July

2011-July 2018. He is the author of 200 papers, 11

patents, 80 invited talks in international conferences. He

is member of Academia Europae. He published the book entitled “An introduction to

microfluidics” (Oxford University Press)

17:00 close

Members and non-members free to chat by text in the main auditorium and visit the

member booths. If you want to be allocated to a particular member booth chat with

Alice.

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17:15 (08:15 PDT) Location Members Executive Lounge. MF 11 Members will

automatically be invited to transfer to the Executive Lounge. They can return to the main auditorium

when they wish by pressing the red ‘leave breakout room’ button to the right of the zoom toolbar.

Session 4: “Microfluidics Hotseat”. Pitches from

early stage microfluidics enabled companies seeking

to engage with the MF11 Consortium

- PhaseLab Instrument – Aurelien Bruyant

Compact and highly sensitive plasmonic sensors have

been developed in our laboratory in collaboration with

two sister startups, Phaselab instrument SAS and

PlasmonicTron. In this talk, I will present the developed

system and its application to the rapid detection of

covid-19 (synthesized virus fragments). We are looking

for partners for the microfluidic delivery system.

- Hummink – Nanoscale Imprinting

Amin M'Barki is one of the founders of Hummink, a start

up from the Micromégas Team at IPGG. Hummink is

working on an nanoscale additive manufacturing

technology, inspired by long-established AFM know-how

in the lab. Hummink is a versatile, compact, adaptable

nanoscale "3D Printer", that can deposit with a resolution

down to 40 nm with, with a xy precision of 1 nm.

As it can deposit any liquid material, Hummink's

technology provides unprecedented versatility and

adaptability for the semiconductor and molecular

diagnostics industries for example.

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- CEA - Dr. Florent Malloggi member of the department of Nanosciences and Innovation for Materials, Biomedicine and Energy, CEA Paris Saclay (UMR 3685).

The fabrication of microfluidic devices itself is still

challenging on many aspects. In particular, the

development of a manufacturing process that satisfies the

need of both academic and industrial actors and is simple

enough to be used by specialists and non-specialists is still

a key issue to be addressed in order to support the

widespread development of lab-on-chip technology in all

fields. Recently, with the emergence of the 3D printing

technology, the hope of being able to quickly and easily manufacture microsystems finally

seemed conceivable.

In this talk, I will present a method to fabricate microchannels based on an inkjet printing of a

volatile solid mold. With this approach it is possible to make microchannels of few tens of

microns width on different nature surfaces such as glass, paper, uncross-linked PDMS layer

or non planar substrates. Moreover this method allows to easily make millimetric height

microfluidic systems.

- NETRI – Microfluidics for Neuroscience. Thibault Honegger.

NETRI has unique expertise and technologies in

microfluidics engineering. NETRI designs, fabricates and

exploits 3D microfluidic chips dedicated to neuroscience,

aiming at understanding the structural and functional

mechanistic of neural circuits, neurological disorders or

potential novel treatments. Using a multi-compartments

approach, we can reproduce in vitro neural networks of

every complexity up to connectome-on-chip. Using rodent

or iPSCs cells, physiologically relevant conditions can be

replicated using NETRI’s NeurofluidicsTM chips:

Multi-nodes neural/non-neural interfaces;

Neural circuit of every complexity;

Controlled structural connectivity;

Functional network recording;

Co-cultures in all nodes with fluid isolation;

Controlled microenvironment with disease related injury;

High throughput microscopy compliance;

Small amount of reagents.

Each talk followed by Q & A hosted by Peter Hewkin. Please raise your hand to ask a

question.

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18:00 Close

Members and non-members free to chat by text in the main auditorium and visit the

member booths. If you want to be allocated to a particular member booth chat with

Alice.

18:15 (09:15 PDT) Location: Main Auditorium:

Session 6 Fluigent Presentation and Demo (Non-member mics on to allow real time Q&A)

Since 2006, Fluigent developed innovative

microfluidic flow control and fluid handling

solutions for laboratories, research units and

industrials around the world. By providing full

control of your microfluidic systems through

pressure, flow and electrical monitoring,

Fluigent technologies provide high end

solutions for all applications. In this session, we

will walk you through the latest Fluigent

developments in terms of fluid control and

automation for 3 key domains and applications:

sequential injection, drug encapsulation and

micro aspiration.

Our video presentation will cover:

1. The use of Negative pressure in microfluidic and applications of interest 2. Automate cellular studies with Aria: Get the success you deserve with Fluigent’s new

perfusion system, Aria that takes care of fluid delivery for you 3. Drug encapsulation in biocompatible microparticles for drug delivery

Q&A will be hosted by Arnaud Cartier.

19:00 Close

Members and non-members free to chat by text in the main auditorium and visit the

member booths. If you want to be allocated to a particular member booth chat with

Alice.

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19:15 (10:15 PDT) Location: Members Executive Lounge MF 11 Members will automatically

be transferred to the Executive Lounge. They can return to the main auditorium when they wish.

Session 6 ParticleGEN: Private discussion for ParticleGEN members only

Led by Richard Gray and Pavel Abdulkin who will report on current status, plans and milestones.

Richard is an executive director of Blacktrace holdings –

with responsibility for Dolomite Microfluidics in the USA.

He will describe the ParticleGEN project which is the

exciting co-development consortium spinning off from

MF11. Enabled by microfluidics technology it will deliver

precision designer coated particles at ton-a-month – for

therapeutic, cosmetic, agricultural and even civil

engineering applications - on a shared cost, shared risk

basis.

Pavel is Chief Commercial Officer at MicroCAPS.

Microcaps enables precision microencapsulation at

industrial scale. We develop formulations, supply

equipment and provide consumables necessary to take

complex microencapsulation projects from proof of

principle to industrial implementation. We work with

pharmaceutical, biomedical and cosmetics companies on

projects such as active ingredient protection, controlled

release and targeted delivery.

20:00 Close

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Delegate List:

Name Organisation Country

Augusto Tentori Douglas Goldstein Jason Li

10X Genomics Adhesives Resea AMF

US US UK

James Howard BD Eire

Pin Chen BD US

Tim Zhu BD US

Christopher Cesar BD US

Philipp Spuhler BD Bioscience US

Richard Gray Blacktrace UK

Callum Marrs Blacktrace US

Florent Malloggi CEA F

Florestan Ogheard CETIAT F

Abir Wissam CETIAT F

Alice Hewkin CfBI UK

Peter Hewkin CfBI UK

Maurice Barakat Cryopak US

Jeffrey Kelly Cryopak US

Cesar Sanchez CSIC E

Mattias Tidare Cytiva S

Darin Latimer Danaher US

Darren Morofke Danaher US

Julian Burke Danaher / Leica UK

Yusuke Harada DestinHaus US

Rahul Ramesh DestinHaus US

Anne Le Nel E-Sana F

Roberta Menezes Eden Microfluidics

F

Bernd Dielacher EV Group A

France Hamber Fluigent F

Nour Yakdi Fluigent F

Mark Aruda Fluigent US

Adam Meziane Fluigent F

Robert Pelletier Fluigent US

Arnaud Cartier Fluigent F

Ching Pin Su Fona US

Norbert Gottschlich Idexcorp D

Alexios Tzannis IMT CH

Mariam Assadian Heidenhain US

Sammy Datwani Inscripta Inc US

Lucie Barbier Institut Curie F

Simon Dumas IPGG F

Amin M’Barki IPGG F

Nahid Hassapour IPGG F

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Jacques Fattaccioli IPGG F

Patrick Tabeling IPGG F

Catherine Villard IPGG F

Remi Wache IPGG F

Koceila Aizel Curie F

Elodie Couttenier IPGG F

Tommaso Fraccia IPGG F

Bruno Pelat IPGG F

Yong Chen IPGG F

Afshin Abrishankar IPGG F

Guilaume Laffite IPGG F

Hongyue Cui IPGG F

Isabelle Eisenmann IPGG F

Lea Pinon IPGG F

Nahid Hassanpour IPGG F

Chaohe Hu IPGG F

Flore Care IPGG F

Josquin Courte IPGG F

Zhibo Li IPGG F

Paul Cochard-Marchewka

IPGG F

Sara Rassouk IPGG F

Tatyana Saleski IPGG F

Julie Signeux IPGG F

Laura Trapiella IPGG F

Marion Gaudeau IPGG F

Mengxue Zhang IPGG F

Andy Racher Lonza UK

Birgit Nelsen Salz Lonza D

Pavel Abdulkin Microcaps CH

Mark Olde Riekerink Micronit NL

Monica Brivio Micronit NL

Willy-An Silvius Micronit NL

Joanna Fielding Pall Corp US

Joost van Beek Philips NL

Robin de Bruijn Philips NL

Aurélien Bruyant Phase Lab Instrument

F

Tsu-Heng Wu Phase Lab Instrument

Taiwan

Marko Nadalin Posalux CH

Cedric Goyer Posalux CH

Bryan Presley Posalux US

Wafa Hecheche Sanofi F

Samin Akbari Sartorius US

David Pollard Sartorius US

Tan Rasab Sigenex UK

Simon Dodd ST Microelectronics

US

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Hans Dijk Surfix NL

Christian Mathis Susos CH

Samuele Tosatti Susos CH

James Oxley SWRI US

Mark McCroy Thermofisher US

William Kim Uni Waterloo Canada

Jessamine Lee Wolf Greenfield US

Sebastian Stoeckeler z-microsystems A

Sonia Smith z-microsystems A

Larry Atupen Zeon Corp US

Hirotaro Fujiki Zeon Corp Japan

Yasuko Horiguchi Zeon Corp Japan

Bold Type indicates MF 11 member

Red type indicates Member Booth delegate

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Logistics:

We are going to use the Zoom platform. Delegates have to register in advance of

participating. Follow the links below for detailed instructions.

- MF11 members register here for free. They can participate in all sessions.

- Non-MF11 members register here. There is a charge and they can only participate in the open sessions.

In the members only session Chatham House Rule applies. Delegates will know who else is

participating in the conversation. Recording is disabled / forbidden. We will also make

breakout rooms available for smaller member meetings. Members should chat with ‘Alice’ if

they have a real time request to talk with someone 1-2-1

You will get the best experience if you use a PC/Laptop (make sure that your mic / camera

work by using the Zoom sound-check feature) – but participation by phone/smart phone is

also possible. We recommend that you keep your video on. If the room gets over crowded we

will mute microphones except for the speaker.

There is no charge for using the Zoom platform – but you might have to pay your

phone/internet company for time/data use.

If you have not used Zoom before, we recommend that you practise. Check out features

like ‘Test mic/video’, ‘Gallery View’/’Speaker View’ ‘Chat’ and ‘Breakout Rooms’. The

participant experience (particularly in the discussions) is best if you enable video .. but this

uses more bandwidth and might cost you more

Main Auditorium:

All delegates land here when they join the meeting. They will usually see this screen

Or the live presentations. They can move to their allocated breakout room whenever they like.

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MF11 Members’ Executive Lounge:

This has been realized using the ‘Breakout Room’ feature in zoom. All MF11 members

have been allocated access to this room. They can use the toolbar breakout room icon

to visit the Executive Lounge and the red ‘leave breakout room’ message to return to

the main auditorium.

MF11 members will automatically be invited to transfer to the Executive Lounge when

Members only sessions are starting.

On arrival in the MF11 Members’ Executive Lounge you will see this screen:

You can return to the Main Auditorium by pressing the red ‘Leave Breakout Room’

button to the right of the toolbar.

Non-MF11 members cannot access the MF11 Members Executive Lounge. But they

can access the member booths.

Members Private Booths:

These have been realized using the ‘Breakout Room’ feature in zoom. The Members

with private booths will have a permanent presence in their room – where they can

hold video discussions with visitors, share screens and show presentations at anytime

they like. Non-member guests will have access to the booth of their host member.

They can use the breakout room icon to visit this private member booth and the red

‘leave breakout room’ message to return to the main auditorium.

At present we expect to have Member Private Booths for:

- Micronit - IMT - Fluigent - z-microsystems - MicroCAPS - Susos - IPGG

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Where a representative of these companies will welcome you at any time.

Delegates and non-delegates wanting to visit a booth other than their assigned

breakout room need to ask the host to change their assigned room. Send a message

by zoom chat to Alice to do this. You will then receive an invitation to join your new

selected Booth.

Questions?:

We know that these processes are sub-optimal and expect some rough edges! Please

contact Peter on ceo@cfbi.com or ++44 7951721110 if you have an urgent problem –

or use the chat feature to contact Alice for less urgent things.