present ss programe anion and cation

8/16/2019 Present SS Programe Anion and Cation

1/15

SHORT- STAY PROGRAM

Laboratory Of Analytical Environmental Chemistry

Faculty of Mathematics and Natural Sciences

Padang-Indonesia

Determination Cation and Anion of BiomaterialDetermination Cation and Anion of Biomaterial

(Straw of Rice)(Straw of Rice)

Using Ion Exchange ChromatographyUsing Ion Exchange Chromatography


2/15

Waste

water

MSL

method

Introduction of My Research

The Multi-Soil-Layering (MSL) system was

composed of soil mixture layers and high

Water permeable layers which are arranged in

brick pattern

It will ca!se a "ariety of the

disease # S$in diseasesCancer %ilariasisDiare etc

If not processedIf not processedbefore usedbefore used

So needSo need


3/15

Introduction of My Research

eolite! high "#" $ absorption of %&'

Soil! filter absorption habitat of organisms

Metal iron! fixation denitrification (reducer)*iomaterial ! denitrification (& donor)

"harcoal! absorption of organic component

habitat of organisms

Material is contained itMaterial is contained it

MSLMSL

T++LT++L

• Determination Cation and Anion ofDetermination Cation and Anion of

BiomaterialBiomaterial

(Straw of Rice) Using Ion Exchange(Straw of Rice) Using Ion Exchange

ChromatographyChromatography

&L"&L"


4/15

Experimental

Apparatus The pump,injector, column capillery fused silica capillary

!"#$mm I% x !"&' mm (%)and the colomn len*ths were +! cm ,and detector"

Rea*ent and material

Stoc solution of-a$S(#,-aI(#,-a.r(#,-a-($,-a-(#,-aI,-aS/- Anions) and0i/l,-a/l,-1&/l,2/0,M*/l$,/a/l$ cation ) ware prepared in +!mM

/oulomn preparation

& 3used silica capillary and paced TS2*el I/4Anion45W60 and I/4

/ation4Sw

Sample

Straw of rice


5/15

Experimental7 Sample preparation for biomaterials

.iomaterials ' *)

3iltrat

' Soa$ed with A!a regia *Cl#*+,-. -#/0(12ml3 45 ho!rs)

' %iltered with filter paper (46m3 d./1 cm)' %iltered with chromatography (2351 6m)

Analysis of or*aniccompound and anion

in biomaterials by 0/


6/15

, -, .,,

.,,,,

',,,,

/,,,,IOIO33

--

A" %etermination of anions in a sample of straw

BrOBrO

33--

NONO22--

NONO

33--

8Min9

+! mM+! mM

/apillary /olumn:Analytes : Standard solution consists ofS(#

$4,I(#4,.r(#4,-($4,-!#4,I4,S/-4

/olumn : TS2 ;el I/4Anion45W60 +!!mmx!,#$ I"% )Eluent : Sodium /hloride #! mM

3low Rate : #? with wa@elen*th ) B

Results and %iscussion

%etermination of Retention Time of Standard

Solution 3rom C*ure + the retention

time of each standardsolution

I(#4,.r(#4,-($

4,-(#4 )

respecti@ely : &"$D& D"&!!+!"F!& +$"'G&) minutes"

I(# more lie a mobile phase

compared to the other anions .efore Ctri et.al ha@e

studied the anion

3i*ure"+

C o m p a r i s o n / r e f e r

e n c e


7/15

Separation of the standard solutionmixture

, ., ', /, 0,

1, m2bs

/apillary /olumn:Sampel : The Standard solution mixture of S(#

$4 +

,I(#4,.r(#4,-($4,-!#4,I4,S/-4

/olumn : TS2 ;el I/4Anion45W60 +!!mmx!,#$ I"% )Eluent : Sodium /hloride #! mM3low Rate : #? with wa@elen*th ) B $'&

+

$

#&

'

F

D


3i*ure $"

The concentrations of the

standard solution mixturewas +! mM

3rom the resultchromato*ram was nownthe capillary columncapable for separatin*

mixtures" The compound anions

was separted respecti@elycontain of S(#

$4 +),I(#4

$),.r(#4#), -($

4&),-(#4

'),I4

F),S/-H

D) The compound number +is not separated perfect, itwould reuire furtherexperiment to Cnd@ariations that are suitable

for separatin* it"


8/15

$ " ' & #

, ., ', /, 0,,

1,,,

-,,,,

/apillary /olumn:Sampel : Straw of rice/olumn : TS2 ;el I/4Anion4 5W60 +!mmx!,#$ I"% )Eluent : Sodium /hloride #! mM3low Rate : #? with wa@elen*th ) B$'&

# " G G +

' "D G G

+ ! " G # &

# # "

# ' !


Aplication of the sample for in@esti*ations

of sample there are 'peas were detected"

It indicates there are

C@e separate

compounds in sample"

the retention time of

each compound

respecti@ely

$,'#"GG+',DGG+!,

G and ##,#G! )

Minutes

Sample were

extracted usin* aua r

Straw of rice

extracted #

a!a regia with a

concentration of /2 m7


9/15

Straw ofrice

/apillary /olumn:Sampel : Standart solution ofS(#

$4,I(#4,.r(#4,-($4,-!#4,I4,S/-4

/olumn : TS2 ;el I/4Anion45W60 +!!mmx!,#$ I"% )Eluent : Sodium /hloride #! mM3low Rate : #? with wa@elen*th ) B $'&

, ., ', /, 0,

-

.

3

'

1

/

4

.1

3 ., m2bs

Mixin*standart


/omparison sample with standardsolution

%rom 1 pea$s are detected of the

sample3 there are - pea$s of the

same retention time with the

standard sol!tion

So from the comparison it3 can

indicated that the compo!nds

contained in the sample straw

are I,-'3Br,-

'3 and +,-' anions8

%or 4 pea$s again not yet $nown

anions3 and need for f!rther

experiment to $now them8


10/15

Standard solution

, ., ', /, 0, -,,

,

,51

-

,1

7


, -, ., 3, ', 1, /,

,

,51

-,

17

." %etermination of cations in a sample of straw

Mixin* standardsolution

/apillary /olumn:Sampel : 0iK,-aK,2 K,M*$K,/a$K

/olumn : I/42ation4 SW +!mmx!,#$ I"% )Eluent : /upper Sulfat +! mM

3low Rate : #? with wa@elen*th ) B

0iK+)

-aK$)

2K#)

M*$K

/a$K

+

$

#

10 mM

%etermination of the retention time of

standard solution

The concentrationsof mix standardsolution was +! mM

3rom the result

chromato*ram wasnown the capillarycolumn capable forseparatin* mixtures"

The retention timesM* and /a cationsare @ery lon*compared with othercations so they arenot used as astandard solution


11/15

NH4+

, ., ', /, 0,

,

,5.

,5'

,5/

,50

-,17


Add +*59

Before add +*59

Separation of the standard solutionmixture

Addin* the ammonium

into the standard

solution mixture can be

nown with the ad@ent

of pea after sodium

cation


/olumn : I/42ation4 SW +!mmx!,#$ I"% )Eluent : /upper Sulfat +! mM3low Rate : #? with wa@elen*th ) B

$+!


12/15

Straw of rice

, , ., 3, ', 1, /,,

,5.

,5'

,5/

,50

,17


A

Extracted #

A8 *Cl / 7

B8 A!aregia /2 m7

B

Application of sample with diLerentextractors

ea!

Aua re*ia as extraction

the straw is better as

compared to hydro*en

chloride

The results showed a

pea in the

chromato*ram of the

straw sample extraction

usin* auare*ia

"#era not

ea!


/olumn : I/42ation4 SW +!mmx!,#$ I"% )Eluent : /upper Sulfat +! mM3low Rate : #? with wa@elen*th ) B$+!


13/15

, ., ', /, 0, -,,,

,5.

,5'

,5/

,50

-,1

7


/omparison sample with standard solution

The result chromato*ram

from comparison standard

solution with sample not

there suitable so that the

cation species contained in

the sample not nown


/olumn : I/42ation4 SW +!mmx!,#$ I"% )Eluent : /upper Sulfat +! mM3low Rate : #? with wa@elen*th ) B

$+!


14/15

A" /onclusion

+" Straw of rice containin* anion I(#4, .r(#4 and-(# 4

$" Auare*ia is as extraction suitable for ricestraw

." Su**etion+" 3urther experiment is needed to determine

anions and cations in straw of rice$" the next experiment is needed modiCcations

to the mobile phase, extract solution, and theaddition of a standard solution as comparison

/onclusion And Su**etion


15/15

present ss programe anion and cation

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