see beyond - gen-lab

SeeBeyond

HALO® and Ascentis® Express

Advanced Materials Technology

Sigma-Aldrich Biotechnology

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See thePerformance Difference

1 Decreased secondary interactions with a more inert base material

2 Sharper peaks for greater resolution

Ranitidine

3 Increased sensitivity (S/N ratio) with taller peaks

Conditions for all columns:Columns: Kinetex® 2.6 µm C18 100 Å

HALO® 2.7 µm C18 90 ÅAscentis® Express 2.7 µm C18 90 Å

Dimensions: 100 x 4.6 mmMobile Phase: A: 40 mM Potassium phosphate, pH 7.1 / Acetonitrile (98:2)

B: 40 mM Potassium phosphate, pH 7.1 / Acetonitrile (78:22)

Flow Rate: 1.5 mL/minTemperature: 35 °C

Detection: UV @ 230 nmSample: Ranitidine amino alcohol hemifumarate1.

Ranitidine diamine hemifumarate (Related compound A)2. Ranitidine S-oxide (Related compound C)3. Ranitidine N-oxide4. Ranitidine complex nitroacetamide5. Ranitidine HCl6.

Gradient: Time (min) % B0 010 10015 100

Conditions for all columns:Columns: Kinetex® 2.6 µm XB-C18 100 Å

HALO® 2.7 µm C18 90 ÅAscentis® Express 2.7 µm C18 90 Å

Dimensions: 100 x 4.6 mmMobile Phase: A: 0.1 % Trifluoroacetic acid (TFA) in Water

B: 0.1 % Trifluoroacetic acid (TFA) in AcetonitrileGradient: Time (min) % B

0 33 323 65

Flow Rate: 1.5 mL/minTemperature: 40 °C

Detection: UV @ 214 nmSample: 1. Insulin

2. Sample Impurity3. Sample Impurity4. Sample Impurity

Insulin Impurity Profiling

HALO is a registered trademark of Advanced Materials Technology, Inc. Ascentis is a registered trademark of Sigma-Aldrich Biotechnology LP. All columns used for comparison were new and manufactured by Advanced Materials Technology, Inc. and Sigma-Aldrich Biotechnology LP, respectively. Phenomenex is in no way affiliated with the above companies. Dimensions and chromatographic conditions are the same for all columns compared unless otherwise noted. Comparative separations may not be representative of all applications.© 2011 Phenomenex, Inc. All rights reserved.

HALO®


HALO®


Ascentis® Express


Ascentis® Express


Kinetex®

Phenomenex®

Kinetex®

Phenomenex®

1538.3 mAU

3

657 mAU

3

553.7 mAU

3

2 Rs (peaks 3 & 4) = 4.41

2 Rs (peaks 3 & 4) = 1.83

2 Rs (peaks 3 & 4) = 1.76

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P ID

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P ID

196

77

1 As = 1.05

0 2 4 6 min0

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mAU

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P ID

196

74

AP

P ID

196

78

1 As = 1.29

0 2 4 6 min0

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mAU

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1 As = 1.16

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P ID

196

73

AP

P ID

196

79

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Phenomenex l WEB: www.phenomenex.com

3

See the Particle Difference

Advanced particle morphology and uniformity

Kinetex® Sub-3 µm Particle HALO® Ascentis® Express2.6 µm Total Particle Size 2.7 µm 2.7 µm

0.35 µm Porous Shell 0.5 µm 0.5 µm1.9 µm Solid Core 1.7 µm 1.7 µm

1000/600 bar* Pressure Limit 600 bar 600 bar100 Å Pore Size 90 Å 90 Å

1.5 – 10** pH Range 2 – 9 2 – 9200 m2/g Effective Surface Area ~225 m2/g ~225 m2/g

Sub-2 µm Particle1.7 µm Total Particle Size - -0.23 µm Porous Shell - -1.25 µm Solid Core - -1000 bar Pressure Limit - -

100 Å Pore Size - -1.5 – 10** pH Range - -200 m2/g Effective Surface Area - -

AP

P ID

187

55 –

1875

7

Nearly monodispersed particle size distribution

Excellent batch-to-batch reproducibility

10

1

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18755

2 3 4 5 min

18756 18757

Batch 68

Batch 73

Batch 75

Kinetex® 2.6 µm C18



3000

2000

Par

ticle

Cou

nt

1000

01 2 3

Particle Diameter (µm)4 5 6

Batch 68 Rs (2,3) 2.00

Batch 73 Rs (2,3) 2.13

Batch 75 Rs (2,3) 2.06

Kinetex® 2.6 µm C18 Core-Shell Traditional Fully Porous C18 1.7 µm HALO® 2.7 µm C18

vs.

Kinetex® DP 90/10 = 1.12

HALO® DP 90/10 = 1.15

HALO is a registered trademark of Advanced Materials Technology, Inc. Ascentis is a registered trademark of Sigma-Aldrich Biotechnology LP. All columns used for comparison were new and manufactured by Advanced Materials Technology, Inc. and Sigma-Aldrich Biotechnology LP, respectively. Phenomenex is in no way affiliated with the above companies. Dimensions and chromatographic conditions are the same for all columns compared unless otherwise noted. Comparative separations may not be representative of all applications.

Phenomenex®


HALO®Advanced Materials Technology

Kinetex®Phenomenex®

Phenomenex®

* 2.1 mm ID Kinetex columns are pressure stable up to 1000 bar.

** Columns are pH stable from 1.5 - 10 under isocratic conditions. Columns are pH stable from 2 - 8 under gradient conditions.

PRODUCT

DOES NOT

EXISTPRODUCT

DOES NOT

EXIST

Advanced Materials Technology Sigma-Aldrich Biotechnology


4

Hydrophobicity:

Polarity:

H-Bonding:

Aromatic Selectivity:

Silanol Activity:

pH Range: 1.5 – 10*USP Classification: L7

Particle Sizes: 2.6 µm & 1.7 µmEffective Carbon Load: 8 %

Low High

C8

TMSTMS

This new phase brings the benefits of core-shell technology to USP L7 and other C8 column methods.

pH Range: 1.5 – 8USP Classification: L43


Hydrophobicity:

Polarity:

H-Bonding:


Silanol Activity:

Low High

PFP

TMS

F

F

F

F

F

TMS

This pentafluorophenyl phase incorpo-rates fluorine atoms on the periphery of the phenyl ring to provide unique aromatic and polar selectivity.

Kinetex PFPHydrophobicity:

Polarity:

H-Bonding:


Silanol Activity:



Low High

XB-C18

Si TMSTMS

This new phase provides superior peak shape and separation of basic compounds.

This unbonded silica phase is used under hydrophilic interaction chromatography conditions to provide the ideal selectivity for the retention and separation of polar compounds.

Hydrophobicity:

Polarity:

H-Bonding:


Silanol Activity:

pH Range: 2.0 – 7.5USP Classification: L3

Particle Sizes: 2.6 µm & 1.7 µm

Low High

HILIC

OO

OHH20

H20H20 H20

H20

H20

H20

H20 H20

H20

H20H20

ACN ACN ACN ACN

ACN ACN ACN ACN

ACN ACN ACN ACN

ACN ACN ACN ACN

HO

HO

OHSi

SiSi

HO

OH

Kinetex HILIC

Hydrophobicity:

Polarity:

H-Bonding:


Silanol Activity:



Low High

C18

TMSTMS

This phase offers the hydrophobic retention and methylene selectivity chromatographers expect from a C18 column.

Kinetex C18

See Amazing Resolution with Complementary Selectivities

* pH range under isocratic conditions. pH range is 2 – 8 under gradient conditions.

Even more than efficiency, selectivity is the most important parameter for obtaining increased resolution.

Tivadar Farkas, Ph.D., Senior Managing Scientist

NEW! Kinetex® XB-C18

NEW! Kinetex C8


2010 R&D Award Recipient

HALO is a registered trademark of Advanced Materials Technology, Inc. Ascentis is a registered trademark of Sigma-Aldrich Biotechnology LP. All columns used for comparison were new and manufactured by Advanced Materials Technology, Inc. and Sigma-Aldrich Biotechnology LP, respectively. Phenomenex is in no way affiliated with the above companies. Dimensions and chromatographic conditions are the same for all columns compared unless otherwise noted. Comparative separations may not be representative of all applications. 5

See What Other Scientists are Saying

1 Increased sensitivity, S/N ratio

2 Narrower peak widths

3 Less tailing

Fig. 7. Comparison between the peak shapes of insulin (A) and lysozyme (B) recorded on the Kinetex and Halo columns.

Reprinted from Journal of Chromatography A, Volume 1217, Issue 10, with permission from Elsevier. “Performance of columns packed with the new shell particles, Kinetex-C18,” page 1598, copyright 2010. By Fabrice Gritti, Irene Leonardis, David Shock, Paul Stevenson, Andrew Shalliker, and Georges Guiochon.

0 min

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90(B)

Ab

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AU

] 205

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From the Journal of Chromatography A

0 min

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CH3CN/H20/TFA, 31/69/0.1, v/v/v 295 K, 1.2 mL/min

CH3CN/H20/TFA, 35/69/0.1, v/v/v

295 K, 0.9 mL/min

1

3

2

Recent publications in the Journal of Chromatography A compare Kinetex® core-shell technology columns to other popular UHPLC technologies.

Articles

1. Performance of columns packed with the new shell particles, Kinetex-C18 Journal of Chromatography A, Volume 1217, Issue 10, 5 March 2010, Pages 1589-1603. By Fabrice Gritti, Irene Leonardis, David Shock, Paul Stevenson, Andrew Shalliker, and Georges Guiochon.

2. Performance of columns packed with the new shell Kinetex-C18 particles in gradient elution chromatography Journal of Chromatography A, Volume 1217, Issue 10, 5 March 2010, Pages 1604-1615. By Fabrice Gritti and Georges Guiochon.

3. Comparative study of new shell type (Kinetex), sub-2 μm fully porous and monolith stationary phases, focusing on mass transfer resistance Journal of Chromatography A, Volume 1217, 4 June 2010, Pages 3642-3653. By Erzsébet Oláh, Szabolcs Fekete, Jenö Fekete, and Katalin Ganzler.

Access these and additional journal articles by visiting www.phenomenex.com/info/journalarticles

Never had such a low reduced HETP value

been achieved in column manufacturing

technology. F. Gritti et al. / J. Chromatogr. A 1217 (2010) 1589-1603

HALO™


HALO™


Kinetex®Phenomenex®

KinetexPhenomenex®


6

Ordering Information

AQ0-8503

AQ0-8530

KrudKatcher Ultra • Extends Kinetex and UHPLC column lifetimes

• Easy to use

• Pressure rated to 20,000 psi (1,375 bar)

• Extremely low dead volume minimizes sample peak dispersion

The KrudKatcher Ultra filter body houses an integrated 0.5 μm 316 stainless steel depth filter that efficiently removes microparticulates from the flow stream without contributing to system backpressure or dead volume (<0.2 μL).*KrudKatcher Ultra requires 5/16 in. wrench. Wrench not provided.


Kinetex 2.6 μm Minibore Columns (mm)KrudKatcher Ultra In-Line Filter*

30 x 2.1 50 x 2.1 100 x 2.1 150 x 2.1 4.6 mm ID/3pk

XB-C18 00A-4496-AN 00B-4496-AN 00D-4496-AN 00F-4496-AN AF0-8497

C18 00A-4462-AN 00B-4462-AN 00D-4462-AN 00F-4462-AN AF0-8497

C8 00A-4497-AN 00B-4497-AN 00D-4497-AN 00F-4497-AN AF0-8497

PFP 00A-4477-AN 00B-4477-AN 00D-4477-AN 00F-4477-AN AF0-8497

HILIC –– 00B-4461-AN 00D-4461-AN 00F-4461-AN AF0-8497

Kinetex 1.7 μm Minibore Columns (mm)KrudKatcher Ultra In-Line Filter*

50 x 2.1 100 x 2.1 150 x 2.1 4.6 mm ID

/3pk

XB-C18 00B-4498-AN 00D-4498-AN –– AF0-8497

C18 00B-4475-AN 00D-4475-AN 00F-4475-AN AF0-8497

C8 00B-4499-AN 00D-4499-AN –– AF0-8497

PFP 00B-4476-AN 00D-4476-AN 00F-4476-AN AF0-8497

HILIC 00B-4474-AN –– –– AF0-8497

Kinetex 2.6 μm MidBore™ Columns (mm)KrudKatcher Ultra In-Line Filter*

30 x 3.0 50 x 3.0 75 x 3.0 100 x 3.0 150 x 3.0 4.6 mm ID/3pk

XB-C18 –– 00B-4496-Y0 –– 00D-4496-Y0 –– AF0-8497

C18 00A-4462-Y0 00B-4462-Y0 00C-4462-Y0 00D-4462-Y0 00F-4462-Y0 AF0-8497

C8 –– 00B-4497-Y0 –– 00D-4497-Y0 –– AF0-8497

PFP 00A-4477-Y0 00B-4477-Y0 00C-4477-Y0 00D-4477-Y0 00F-4477-Y0 AF0-8497

HILIC –– –– –– –– 00F-4461-Y0 AF0-8497

Kinetex 2.6 μm Analytical Columns (mm)KrudKatcher™ Ultra In-Line Filter*

30 x 4.6 50 x 4.6 75 x 4.6 100 x 4.6 150 x 4.6 4.6 mm ID/3pk

XB-C18 –– 00B-4496-E0 00C-4496-E0 00D-4496-E0 00F-4496-E0 AF0-8497

C18 00A-4462-E0 00B-4462-E0 00C-4462-E0 00D-4462-E0 00F-4462-E0 AF0-8497

C8 –– 00B-4497-E0 00C-4497-E0 00D-4497-E0 00F-4497-E0 AF0-8497

PFP 00A-4477-E0 00B-4477-E0 00C-4477-E0 00D-4477-E0 00F-4477-E0 AF0-8497

HILIC –– 00B-4461-E0 00C-4461-E0 00D-4461-E0 00F-4461-E0 AF0-8497

UHPLC / HPLC Sure-Lok™ High Pressure PEEK™ Male Nut FittingsPart No. Description UnitAQ0-8503 Sure-Lok High Pressure PEEK 1-Pc Nut 10-32,

for 1/16 in. Tubing, 12,000 psi (827 bar) 10/pk

AQ0-8530 Sure-Lok Fitting Tightening Tool, Aluminum ea

t: f:

Australia02-9428-6444 02-9428-6445 [email protected]

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Austria 01-319-1301 01-319-1300 [email protected]

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All other countries: Corporate Office USA (310) 212-0555 (310) 328-7768 [email protected]

www.phenomenex.comPhenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at [email protected]

Terms and Conditions Subject to Phenomenex Standard Terms & Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions.

Trademarks Kinetex and Phenomenex are registered trademarks of Phenomenex in the United States, European Union, and other jurisdictions. MidBore, KrudKatcher, and Sure-Lok are trademarks of Phenomenex, Inc. HALO is a registered trade-mark of Advanced Materials Technology, Inc. Ascentis is a registered trademark of Sigma-Aldrich Biotechnology LP. PEEK is a trademark of Victrex plc.

Disclaimer Phenomenex, Inc. is in no way affiliated with Advanced Materials Technology, Inc. or Sigma-Aldrich Biotechnology LP. Comparative separations may not be representative of all applications.

© 2011 Phenomenex, Inc. All rights reserved.

If you are not completely satIsfIed wIth KInetex core-shell columns, send In your comparatIve data to a sImIlar product wIthIn 45 days and Keep the column for free.

BR

7587

0510

_I

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