standardization the biometry, reproduction and ...€¦ · romania in the period 1982-1997: •102...

Sectorial program ADER 2015-2020funded by:

MINISTRY OF AGRICULTURE AND RURAL DEVELOPMENT www.madr.ro

PROIECT ADER 9.1.1./28.10.2015

Standardization the biometry, reproduction and instrumental insemination methods useful for honeybee colony evaluation, with applicability in preservation and

breeding activities of national beekeeping genetic background

SC INSTITUTUL DE CERCETARE-DEZVOLTARE PENTRU APICULTURĂ SA

www.icdapicultura.ro, www.aca.org.ro,

http://www.madr.ro/http://www.icdapicultura.ro/http://www.icdapicultura.ro/

Institutions:

Coordinator:

SC INSTITUTUL DE CERCETARE-DEZVOLTARE PENTRU APICULTURĂ SA -ICDA SA - Director, eng. Căuia Eliza, PhD

Partner:

UNIVERSITATEA DE ȘTIINTE AGRICOLE ȘI MEDICINĂ VETERINARĂ A BANATULUI ”REGELE MIHAI I AL ROMÂNIEI“, DIN TIMIȘOARA –USAMVBT. – Responsible, prof. eng. Pătruică Silvia, PhD

Project objectives:

1. Standardization of a method for biometric evaluation of local bee (A.m. carpatica) with references for other European honeybee races (2018).

2. Optimization and standardization of rearing queens and drones methods, with applicability in breeding apiaries (2015-2016).

3. Optimization of technology for queen instrumental insemination (2017).

Objective no. 1: Standardization of a method for biometric evaluation of local bee (A.m. carpatica) with references for other European honeybee races (2018).

• The use of DrawWing method developed by Adam Tofilsky (Polish method)

• The biometric analyses by this system is based on the following stages:

• Collection of forewing in samples of minimum 20 bees/apiary, in Eppendorf tubes;

• Scanning the collected wings by the specific device, storing and codding them for a better identification;

• Automatic measurements by DrawWingPro.exe, according to the references (photo no. 1)

• Manual correction of the errors;

• Classification of analysed samples by IdentiFly program – in the phylogenetic lineages and races of A. mellifera.

Photo. no. 1. The system of points references used to make

automatic measurements for geometric analysis in honeybees by

DrawWing program.

• The references samples collected from Romania in the period 1982-1997:

• 102 samples in 28 locations (counties) totalling 3491 processed wings out of 3533 scanned.

Photo. no. 2. Romania map where the references (older) samples location is marked (1982-1997 samples).

Photo. no. 3. Romania map where the analysed (newer) samples location is marked (2016 samples).

• The analysed samples collected in 2016:

• 64 samples in 32 locations (counties) totalling 1800 processed wings out of 1827 scanned.

Objective no 1: Standardization of a method for biometric evaluation of local bee (A.m. carpatica) with references for other European honeybee races (2018).

• The graphic representation of the distribution of honeybee samples analysed by IdentiFly program, in order to classify the reference samples (photo no 4) and the analysed samples (photo no 5) in the frame of geographical races registered in the system.

• Details with all the wings samples.

• A large variability of points positioning can be seen, this fact conducting to the idea that this programme can be used only with scientific purpose in order to find out the population average

Photo. no. 4 Photo. no. 5


• The graphic representation of the distribution of honeybee samples AVERAGE (the black point in the carnica circle) analysed by IdentiFly program, in order to classify the reference samples (photo no 6) and the analysed samples (photo no 7), in the frame of geographical races registered in the system.

• This result show that both reference samples and analysed samples averages are different which show that the Romanian bee population is a distinct race, as its average is not overlapping on the other populations averages.

Photo. nr. 6. Photo. nr. 7.


Conclusions:• The DrawWing/Identifly programs represent a relative rapid method to classify the honeybee populations in

Romania and other surrounding populations, in the phylogenetic and races distribution.

• The present study , confirm the researches of classic morphometry and molecular biology which show significant differences of Romanian bee population as compared with other honeybee races spread in the studied area, justifying the taxonomic registering as a distinct population in the geographic races distribution.

• By this discrimination it is completely justified the use of name A.m. carpatica, firstly published at international level by Foti N. 1965.

• This study is confirming once more time the necessity for specific measures to conserve and improve the race A.m. carpatica, as a standalone geographic race which is different as compared with A.m. carnica or other races from Central and South-East Europe.

• Classification of honeybees’ samples by this program is relevant especially at the level of population, by collecting and processing a high number of samples, with scientific purposes in large areas discrimination studies.

• At present, the application of this method need more studies, and cannot be generalized in the beekeeping practices to discriminate samples of honeybees which come from beekeepers at their request. Generally, all the known methods, including the molecular ones are relevant on large numbers of samples.

• The researches in this field should continue by international cooperation in order to use comparative approaches on more samples from different regions or countries in Europe.

Evolution of crude protein level in bee’s body collected from colonies with different level of development in the winter bees’ formation and early in spring

Table no 1. Values (min., max., ave.), regarding the level of crude protein in the bees body - August-September 2015 and March 2016 Tabel nr. 4. Valori minime, maxime si medii privind nivelul proteinei crude din corpul

albinelor in perioada august - septembrie 2015 și martie 2016.

Perioada August Septembrie Martie

Min 25,1 34,46 32,24

Max 62,82 69,37 49,53

Medie 50,64 48,28 40,86

N 30 30 13

Objective no 2: Optimization and standardization of rearing queens and drones methods, with applicability in breeding apiaries (2015-2016).

• Identification of nutritional status of honeybee colonies using the crude proteins analyses in the bee body in order to understand the capacity of honeybee colonies to rear queens and drones

Evolution of crude protein level in bees’s body collected from colonies with different level of development, in active season, April, May, June 2016

The crude protein level in nurse bees in the testing period

The crude protein level in pollens collected in spring, summer and in

autumn


Morpho-phsiological quality parameters, for mated/virgin queens resulted from specific measurements.

VALUES Weight

(mg)GenalIndex

Length/width (mm)

The

presence of

mandibular tooth

Mandibular index

Tarsian index

3rd pair of feet

Length/ width(mm)

Pollen comb presence

Number of

barbes on the sting

Mated queens

Virgin queens

MINIM 196 141 1.04 + 3,27 2,05 + 1

MAX 290 222 1,10 + 3,57 2,32 + 3

AVERAGE 242,3 178 1,076 + 3,4 2,15 + 2


• Some morphological aspects regarding

differences between queen and honeybees

Diferențe morfologice între caste

MATCA ALBINA LUCRĂTOARE

Matcă –rotunjit Albina lucrătoare -triunghiular

Forma capului

Matca + Albina lucrătoare -

Prezența dintelui mandibular

Matca - Albina lucrătoare +

Prezenta periei de polen

Matca – îndoit Albină lucrătoare- drept

Forma acului


• Institutul de Cercetare Dezvoltare pentru Apicultură publishedThe DVD - Queen rearing in an intensive system, 2017, FullHD video material, 67 min, in

Romanian and English.


• Participation to APIMONDIA Congress - in the period 29 sept. - 04 oct. 2017, in the contest World Beekeeping Awards, Publications, obtaining:

The golden medal


Modernization the ICDA laboratory for instrumental insemination

Objective no. 3: Optimization of technology for queen instrumental insemination (2017).

Profesional formation courses- Initiation in the practice of instrumental insemination in the period May 30-31th 2018 and June 6-7th 2018:

Objective no. 3: Optimization of technology for queen instrumental insemination (2017).

• Institutul de Cercetare Dezvoltare pentru Apicultură

1. MANUAL DE ÎNSĂMÂNȚARE INSTRUMENTALĂ LA ALBINE (A. mellifera) (Handbook for

instrumental insemination in honeybees –A. mellifera)

Authors: Eliza Căuia, Adrian Siceanu.

Publishing House: Editura Universitară

(acreditated CNCS), 2017, 118 pag.

ISBN 978-606-28-0649-1,

DOI: 10.5682/9786062806491.

Books:

• USAMVBT

2. ÎNSĂMÂNȚAREA ARTIFICIALĂ CA METODĂ DE AMELIORARE A ALBINELOR. (Instrumental insemination as method in honeybee breeding)Authors: Silvia Pătruică, Marian Bura.Publishing house/Editura Eurobit, Timișoara, 2017, 366 pag.ISBN 978-973-132-407-4

3. Queen rearing – Practical guide for students.Author. prof. dr. ing. Silvia Pătruică, Publishing house/Editura Eurobit, Timișoara, 2016,

Books:

Other relevant dissemination activities

Conferences for beekeepers at national by Romanian Beekeepers Associations and other associations: Technology of queen rearing, The importance of preservation and breeding of national beekeeping genetic background- some measures for preventing the degradation and the Presentation of Queen rearing in an intensive system - video film, conferences presented by Eng. Adrian Siceanu, PhD.

Participation in scientific events:

The International Scientific Symposium „Bioenginery of Animal Resources 2016” –organized by the Animal Breeding and Technologies Faculty – Timişoara, May 26-27 2016,

The Scientific Symposium with International participation “60 years since the foundation of institute”IŞPBZ Maximovca, Republic of Moldova, September 29th - October 1st 2016.

The international Conference of Apidology – EURBEE 2016, (http://eurbee7.usamvcluj.ro/) Cluj Napoca, September 7-9th 2016.

Scientific articles in Romania Apicola magazine (ISSN 1220-2525),

Nutrition Management (Managementul nutriției – impactul polenului asupra dezvoltării și sănătății

familiilor de albine, (no. 10,11,12 - 2016).

Instrumental insemination (Însămânțarea instrumentală a mătcilor – o abordare utilă și de perspectivă în

controlul împerecherilor la albina meliferă), no 9 - 2017.

Improving the activity for breeding and conservation of Romanian beekeeping genetic background - A.m. carpatica:

Improoving the legislative and educational framework with some measures:

• INTERDICTION in România of other races and interracial hybrids by reglementation the system of control and sanctions in order to discourage this activity;

• IMPROVING the National Program for honeybees conservation and breeding of Romanian honeybees by improving the breeding pyramid (by introducing a new category RECORD between ELITE and MULTIPLICATION for independent breeding apiaries in order to stimulate the breedin activity in the local population - A.m. carpatica.

• IMPROVING of National Program for honeybees conservation and breeding of Romanian honeybees, by stimulation the setting-up of the mating stations.

• SUBSIDIARIES of breeding apiaries for selection activity.

• FUNDING technical asistance and formation courses in breeding activity.

standardization the biometry, reproduction and ...€¦ · romania in the period 1982-1997: •102...

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