state of the art on phaseolus vulgaris …...l02 pisum sativum conv. speciosum 91 l03 vicia sativa...

STATE OF THE ART

ON PHASEOLUS VULGARIS AND P. COCCINEUS

IN SLOVAK NATIONAL COLLECTIONS

Pavol Hauptvogel National Agricultural and Food Centre – Research Institute of Plant Production

Bratislavská cesta 122, 921 68 Piešťany, Slovak Republic

SMARTLEG:

Efficient Management of Genetic Resources for Smart Legumes Utilization

25-26 April 2017, Agricultural Institute of Slovenia, Ljubljana, Slovenia

• established in year 1996,

• specific technical institution for medium-

term and long-term storage of the seeds,

• aims and needs of National Programme on

Conservation of PGR for FA,

• Act No. 215/2001 on Conservation of PGR

for FA and Regulation No. 238/2006,

• Activity: • gathering and evaluation;

• conservation of ex situ, in vitro and field

collections;

• acquisition of rare foreign genotypes;

• creation of passport and descriptive

databases;

• offering biological material for breeding,

research, study ...

SVK001

GENE BANK OF THE SLOVAK REPUBLIC

Front office: http://griss.vurv.sk/

Back office: http://kurator.vurv.sk

SMARTLEG:



http://griss.vurv.sk/



http://kurator.vurv.sk/



STEP BY STEP IN GENE BANK OF SR

• Monitoring of PGR

• Collecting mission

• Evaluation of PGR

• Methodology of different crops groups:

preliminary evaluation

basic evaluation - morphological,

economic characteristics and health status,

special evaluation – technological quality,

phytopathology and mol. markers.

• Documentation and inventory of PGR

• Regeneration of PGR

• Distribution and information providing of PGR

SMARTLEG:



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CONSERVATION OF PLANT GENETIC RESOURCES IN GENE BANK OF SR:

180 ex situ collections

1 in vitro collections

1 in situ collections

SMARTLEG:



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The experimental fieldin Piešťany

CONSERVATION OF PLANT GENETIC RESOURCES IN GENE BANK OF SR:

• 22 260 conserved accessions and 26 679

accessions with field collections

• 18 368 accessions in active collection,

• 3 896 accessions in basic collection,

• 3 686 accessions in the security collection in GB

RIPP Praha and 2236 acc. from CZE in GB SR,

• 75 accessions of hops in vitro

SMARTLEG:



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USING OF GENETIC RESOURCES ACCESSIONS IN GB SR

SMARTLEG:



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STATUS OF PULSES COLLECTION IN GB SR

SMARTLEG:



INSTCODE Genus Σ

L01 Pisum sativum 693

L02 Pisum sativum conv. speciosum 91

L03 Vicia sativa 62

L04 Vicia faba 59

L05 Phaseolus vulgaris, coccineus, acutifolius, lunatus and angularis 1337

L06 Glycine max 617

L07 Lupinus luteus, angustifolius and albus 61

L08 Lens culinaris 292

L11 Cicer arietinum 318

L14 Lathyrus sativus 63

L17 Vicia villosa 1

ΣΣ 3594

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STATUS OF SAMPLES COLLECTION - SAMSTAT

SMARTLEG:



Species Σ 100

Wild

200

Weedy

300

Traditional

cultivar

landrace

400

Breedings

research

material

500

Advanced

improved

cultivars

P. acutifolius 2 1 1

P. angularis 1 1

P. coccineus 54 49 5

P. lunatus 6 1 5

P. vulgaris 1271 659 55 556

P. ssp. 3 2

Σ 1334 710 55 568

300

Traditional

cultivar/la

ndrace

53% 400

Breedings

/research

material

4%

500

Advanced

/improved

cv.

43%

SMARTLEG:



P.

acutifoli

us

0%

P.

angulari

s

0%

P.

coccineu

s

4%

P.

lunatus

1%

P.

vulgaris

95%

P.ssp.

0%

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TYPES OF BEAN GENETIC RESOURCES - %

INSTCODE ACCENUMB COLLNUMB GENUS SPECIES

100 % 100 % 100 % 100 % 100 %

SPAUTHOR SUBTAXA ACCENAME ORIGCTY COLLSITE

99,87 % 94,99 % 100 % 100 % 42,11 %

LATITUDE LONGITUDE ELEVATION COLLDATE SAMPSTAT

0,15 % 0,15 % 40,69 % 40,39 % 99,43 %

ANCEST COLLSRC DONORCODE STORAGE

6,13 % 100 % 91,02 % 99,95 %

SUMMARY OF PASSEPORT DATA BEAN(MCPD V2) MANDATORY SVK001

SMARTLEG:



SUMMARY OF C&E DATA BEAN SVK001

SMARTLEG:



Stem-height of bushy

bean

Stem-height of climbing

bean Pod-width Pod-width

100,00 96,25 100,00 86,65

Pod-lenght immature

determinant

Pod-lenght immature

indeterminant Pod-beak lenght Pod-beak lenght

100,00 86,65 95,39 65,57

Plant-height setting of the

first pod

Plant-number pods per

plant Seed-ground colour Seed-ground colour

66,04 65,89 97,42 4,84

Seed-ground colour Seed-second colour

darker colour

Seed-second colour

darker colour

Seed-second colour

darker colour

39,03 96,57 0,70 30,52

Seed-thousand-kernel

weight

Seed-crude protein

content in dry matter Seed-cooking ability

Seed-uniformity of

cooking

100,00 100,00 84,07 84,07

Lodging-resistance Virus diseases Bacteriosis

Colletotrichum

lindemutianum SACC. et

MAGN/BR. et CAV

97,89 98,44 98,44 98,44

Fusarium solani f. phaseoli

(MART.) APP. et WR.

Uromyces phaseoli (PERS.)

WINT

Vegetation period -

sowing - beginning of

flowering

Vegetation period -

beginning of flowering-

end of flowering

98,44 98,44 89,85 89,77

NEXT OF C&E DATA BEAN SVK001

SMARTLEG:



Plant-Type of germination Pod -frequency of pigment spots

Plant-anthocyan pigmentation of hypocotyl Pod-beak position

Plant-gowth type Pod-neck in the time of ripeness

Plant-habit Pod-wall fibre

Leaf-shape of middle leaflet Pod-colour /mature/

Leaf-colour of middle leaflet Plant-height setting of the first pod

Leaf-surface of middle leaflet Plant-number pods per plant

Inflorescence-location only determinant Pod-number of seeds per pod

Flower-size of bracteole Seed-shape

Flower-vexillum colour Seed-character of pattern

Flower-wings colour Seed-brilliance

Pod-cross section Seed-hilum ring colour

Pod-curvature Seed-lenght-measured by hilum

Pod-parchment coating Seed-height-measured through of hilum

Pod-presence of fibre Seed-width-measured through sides

Pod-ground colour Vegetation period - sowing - maturity

Pod-pigmentation Main use

Pod-colour of pigment spots Seed-biomolecular characteristic

CONSERVATION AND SUSTAINABLE USE OF PLANT GENETIC RESOURCES:

• to promote innovative approaches,

• necessary systemic measures at all levels,

• the conservation and utilization of plant genetic resources of all

participants within the meaning of applicable international

agreements.

SMARTLEG:



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UNDERSTANDING THE USE OF PGR:

→determine their value,

→ stimulate their permanent

coonservation

→ sustainable use

THE INCREASED INTEREST IN ACCESSIONS OF GR:

• research purposes and educational

activities,

• local plants genopool of Slovak or

Czechoslovak origin

• historical and cultural heritage.

SMARTLEG:



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SMARTLEG:



Thanks

Co-author of contribution and colleagues:

Da iela Be ediková, Mi haela Be ková, Ľu o ír Me del and Eva Galbavá

and all colleagues in the creation of collections and

conservation of plant genetic resources!

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THANK YOU FOR YOUR ATTENTION

SMARTLEG:



Contact: [email protected]

mailto:[email protected]

state of the art on phaseolus vulgaris …...l02 pisum sativum conv. speciosum 91 l03 vicia sativa...

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