Supplementary Figures

Normal CRC CRC+5FU CRC+CPT11

100µm100µm100µm100µm

S1

Normal CRC CRC+5FU

CRC+CPT11

Gr-

1+ C

D11

b+ c

ells

(%

)

**NS

Normal CRC CRC+5FU

CRC+CPT11

Gr-

1+ C

D11

b+ c

ells

(%

)

**

**

S2

Normal CRC CRC+5FU

CRC+CPT11

CD

247

(MF

I)

*NS

Normal CRC CRC+5FU

CRC+CPT11

CD

247

(MF

I) **NS

A

B

Normal CRC CRC+5FU

CRC+CPT11

CD

4+ F

oxp3

+ c

ells

(%

)

*

S3

S4

NO

- pr

oduc

tion

(MF

I) ** *

M-MDSCs G-MDSCs

MF

I **

ROSNO-

NS

NS

A B

C

**NS

CD

8+ c

ell

prol

ifera

tion

(%)

Normal CRC CRC+5FU

CRC+5FU+

CPT11

CRC+CPT11

****

No drug5FUCPT115FU+CPT11

*

M-MDSCs G-MDSCs

RO

S

prod

uctio

n (M

FI)

*

21.88%

65.06%

41.78%

19.13%

24.08%

Normal

CRC

CRC+5FU

CRC+CPT11

CRC+5FU+CPT11

CFSE

Cel

l cou

nt

Non-activated

7.11%

D

*NS

CD

247

expr

essi

on (

MF

I)

Normal CRC CRC+5FU

CRC+5FU+

CPT11

CRC+CPT11

w1 w2 w3 w4 w5 w6 w7 w8 w9 w10 w11

Har

vest

AOM AOM DSS DSS

Anti-Gr1 mAb injections

S5

A B

S6

Cle

aved

cas

pase

-3 (

MF

I)

Normal CRC CRC+5FU

CRC+CPT11

CRC+5FU+

CPT11

Cle

aved

cas

pase

-3 (

MF

I)

Normal CRC CRC+5FU

CRC+CPT11

CRC+5FU+

CPT11

Cle

aved

cas

pase

-3 (

MF

I)

****

*

M-MDSCs G-MDSCs

No drug5FUCPT115FU+CPT11

CRC

CRC+5FU

CRC+CPT11

CRC

CRC+5FU

CRC+CPT11

*

NS

*

** S100A8S100A9

mR

NA

(R

Q)

5FU µmol/L

mR

NA

(R

Q) *

CPT11 µmol/L

NS

*

mR

NA

(R

Q)

S100A9

CPT11 µmol/L

NS

mR

NA

(R

Q)

5FU µmol/L

**

mR

NA

(R

Q)

TNFαA

C

B

S7

E

A

Gr-

1+ C

D11

b+ c

ells

(x1

06)

**NS

Inflamed5FUCPT11

- + + + + - - + - + - - - + +

B

CD

4+ F

oxp3

+ c

ells

(%

)

***

Inflamed5FUCPT11

- + + + + - - + - + - - - + +

S8

Rel

ativ

e C

D24

7 ex

pres

sion

(%

)

***

Inflamed5FUCPT11

- + + + + - - + - + - - - + +

R

elat

ive

CD

247

expr

essi

on (

%)

**

Inflamed5FUCPT11

- + + + + - - + - + - - - + +

R

elat

ive

CD

247

expr

essi

on (

%)

*NS

Inflamed5FUCPT11

- + + + + - - + - + - - - + +

D

C

CD

8+ c

ell

prol

ifera

tion

(%)

NS

Inflamed5FUCPT11

- + + + + - - + - + - - - + +

***