twosex-mschartcan be used on four ways to run twosex

Microsoft PowerPoint - 00-How to use TWOSEX.pptxCopyright 1997-2020 Prof. Dr. Hsin Chi 1
Chapter 2 Life table analysis using TWOSEX-MSChart
TWOSEX-MSChart ( Basic method)
Prof. Dr. Hsin Chi
Adjunct professor, National Chung Hsing University Adjunct professor, Fujian Agriculture and Forestry University
Adjunct professor, Shandong Agricultural University
Download new version of TWOSEX-MSChart http://140.120.197.173/Ecology/prod02.htm
Copyright 1997-2020 Hsin Chi 2
TWOSEX-MSChart can be used on Windows and Mac system
TWOSEX has been used on Windows XP, 7, 8, 10. In most cases, there will be no problem.
If you use Chinese or non-ASCII codes for the folder names, you may encounter some problems. But you can always find a solution for that. Only in extreme cases, you may have to re-install your operating system.
On Mac computer, you have to use Windows operating system to run TWOSEX.
Four ways to run TWOSEX 1. Extract TWOSEX-MSChart-exe-B100000.rar, run
TWOSEX-MSChart.exe (or similar) as administrator.
2. Extract TWOSEX-MSChart-B100000.rar, run setup.exe. Then run TWOSEX as a Windows program.
3. Extract TWOSEX-MSChart-B100000.rar and put it on Desktop, run the program directly from the Support folder. (Run as administrator).
4. In rare cases, you have to install OCX (active control).
1a. Open folder 0-Programs Run TWOSEX-MSChart.exe
1b. TWOSEX-MSChart-exe.rar
A. If you download TWOSEX-MSChart-exe.rar, there is only one file TWOSEX-MSChart.exe, just click it to run. You don’t need to setup the program.
B. If you face problems, please click the right mouse key and try to run TWOSEX.exe “as administrator”.
Chapter 2. TWOSEX-MSChart (Basic) 12/7/2020
Copyright 1997-2020 Prof. Dr. Hsin Chi 2
2. TWOSEX-MSChart-B100000.rar Standard setup procedure
Double click on setup.exe and follow the red arrow
Click this button to install
Just click on Continue
If you see this, just click Yes.
Setup was successfully
3. Run TWOSEX-MSChart.exe from the Support folder.
If you see this message, you can extract the RAR file and put it on the Desktop. Then you can run the program from the Support folder.
OK (Turkish)
If you cannot setup at all, If you are using Windows XP, you
may face trouble and will not be able to setup the program.
Extract TWOSEX-MSChart.rar to a new folder on your Desktop.
Open the Support folder, find the file TWOSEX-MSChart.exe.
Use right mouse key to click on it.
Select “Run as administrator”.
4. Register OCX component to your computer ()
If you cannot run TWOSEX-MSChart.exe and you see this message (or similar), then you have to
install the OCX file in order to run the program.
How to register an OCX file You can find MSFLXGRD.OCX (and other OCX files)
on the internet. Save them to C:\windows\system32 (or syswow64) folder.
You need regsvr32.exe and regedit.exe (or regedt32.exe). If you cannot find them in your computer, you can download them from internet.
Start the command mode: C:>
Go to C:\Windows\system32 (or syswow64).
Run C:>Regsvr32 MSFLXGRD.OCX. If it shows “successfully installed”, you can continue.
Run C:>Regedit vbctrls.reg or C:>regedt32 vbctrls.reg
OCX OCX (
MSFLXGRD.OCX). C:\windows\system32 ( syswow64) .
regsvr32.exe regedit.exe ( regedt32.exe).
C:>_
C:>Regsvr32 MSFLXGRD.OCX. “successfully installed”, .
C:>Regedit vbctrls.reg C:>regedt32 vbctrls.reg
OCX Copyright 1997-2020 Hsin Chi 17
If you use TWOSEX, you must cite the following references
TWOSEX3
Chi, H., and H. Liu. 1985. Two new methods for the study of insect population ecology. Bull. Inst. Zool., Acad. Sin. 24: 225-240.
Chi, H. 1988. Life-table analysis incorporating both sexes and variable development rates among individuals. Environ. Entomol. 17: 26-34.
Chi, H. 2020. TWOSEX-MSChart: a computer program for the age- stage, two-sex life table analysis. National Chung Hsing University, Taichung, Taiwan, (http://140.120.197.173/Ecology/Download/ Twosex- MSChart.zip) (accessed 1 August 2020). (Replace 2020 with the actual year you downloaded the program) ( ).
Enter username.
If you are using an old version, you have to download the new one.
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2
3
4
3
Check “I have read and accepted the terms and conditions”
4
If the password is correct, you can see the main page.
You have to collect 1. Stage developmental duration of each individual. 2. sex of each individual. 3. Daily
fecundity of all females.
No. Sex Developmental duration Daily fecundity
Egg Larva Pupa Adult 1 2 3 4 5 6 7 8 9
1 M 6 11 12 6
2 F 6 10 11 10 0 124 12 0 4 2
3 F 6 11 10 10 0 22 74 13 0 1
4 F 6 12 10 7 0 97 28 1 4 0
5 M 6 12 11 5
6 F 6 12 11 14 0 61 11 7 15 15 2 3 4
7 M 6 13 10 8
.. .. .. .. .. ..
.. .. .. .. .. ..
20 N 6 -10
Write data for individuals no. 1 and 2
1,M,6,11,12,6 2,F,6,10,11,10,0,124,12,0,4,2,0,0,0,0,-1 or 1,M,6,11,12,6 2,F,6,10,11,10,0,124,12,0,4,2,-1 or 1,M,6,11,12,6 2,F,6,10,11,10 0,124,12,0,4,2,-1
Developmental time and fecundity in separate lines. .
Detailed explanation of data format "Project: Diamondback moth at 25C" "User: Ta-Chi Yang" "DBM." 50 (Number of eggs used at the beginning) 3,7 (Number of types, number of stages) F, Egg, L1,L2,L3,L4,Pupa,Female (Female stages) M, Egg, L1,L2,L3,L4,Pupa,Male (Male stages) N, Egg, L1,L2,L3,L4,Pupa,Unknown (Unknown type) L1,L4 (Special grouping: total time from L1 to L4) 1,M,7,3,4,5,6,5,8 (Developmental time) 2,F,7,2,3,4,5,8,6,4,12,9,5,8,0,-1 (Daily fecundity) 3,N,7,-7 (Died on 7th day of L1) 4,F,6,2,3,4,5,7,19,0,0,2,20,9,6,5,10,8,13,10,12,9,0,0,-1
Don’t use “º” or any non-ASCII code.
Data format for 7 developmental stages "Project: Silverleaf whitefly at 25C" "User: Hsin Chi" "25C" 50 3,7 F, Egg, L1,L2,L3,L4,Pupa,Female M, Egg, L1,L2,L3,L4,Pupa,Male N, Egg, L1,L2,L3,L4,Pupa,Unknown L1,L4 1,M,7,3,4,5,6,5,8 2,F,7,2,3,4,5,8,6,4,12,9,5,8,0,-1 3,N,7,-7 4,F,…….
Developmental time for each individual and fecundity for each female
Descriptive data and definition
Treatment code. As short as possible!
Data format for 7 developmental stages "Project: Silverleaf whitefly at 25C" "User: Hsin Chi" "25C" 50 3,7 F, Egg, L1,L2,L3,L4,Pupa,Female M, Egg, L1,L2,L3,L4,Pupa,Male N, Egg, L1,L2,L3,L4,Pupa,Unknown L1,L4 1,M,7,3,4,5,6,5,8 2,F,7,2,3,4,5,8,6,4,12,9,5,8,0,-1 3,N,7,-7 4,F,…….
This line asks the program to calculate the total larval duration.
Don’t use “”.
Data format for 2 developmental stages "Project: Silverleaf whitefly at 30C" "User: Hsin Chi" "30C" 50 3,2 F, Immature, Female M, Immature, Male N, Immature, Unknown Immature, Female 1,M,30,8 2,F,29,6,4,12,9,5,8,0,-1 3,N,-7 4,F,…….
You must have this line.
Data format for Parthenogenetic populations
"Aphis gossypii" "Shally" "25C" 56 2,5 F,N1,N2,N3,N4,Female N,N1,N2,N3,N4,Unknown N1,N4 1,F,2,1,3,1,18,0,9,0,11,12, ...,-1 ...... 48,N,1,3,-1 ......
Data format for Parthenogenetic populations (: F, M, N)
"Aphis gossypii" "Shally" "25C" 56 3,5 F,N1,N2,N3,N4,Female M,N1,N2,N3,N4,Male N,N1,N2,N3,N4,Unknown N1,N4 1,F,2,1,3,1,18,0,9,0,11,12, ...,-1 ...... 48,N,1,3,-1 ......
Data format for 7 developmental stages with stage-specific rearing cost ()
"Project: Diamondback moth at 25C"
"User: Hsin Chi"
0, 0.2, 0.3, 0.5, 1, 0, 2, 1.5 L1,L4
The rearing cost for egg, L1, L2, L3, L4, Pupa, Female and Male adults are 0, 0.2, 0.3, 0.5, 1, 0, 2, and 1.5, respectively.
Not well! There are 8 data. 2 is the rearing cost of female and1.5 is the cost of male.Copyright 1997-2020 Hsin Chi 34
7 "Project: Diamondback moth at 25C" "User: Hsin Chi" "DBM" 50 3,7 F, Egg, L1,L2,L3,L4,Pupa,Female M, Egg, L1,L2,L3,L4,Pupa,Male N, Egg, L1,L2,L3,L4,Pupa,Unknown
0, 0.2, 0.3, 0.5, 1, 0, 2, 1.5 L1,L4
The rearing cost for egg, L1, L2, L3, L4, Pupa, Female and Male adults are 0, 0.2, 0.3, 0.5, 1, 0, 2, and 1.5, respectively.
8
Attention! Do NOT enter more than 30 data
items in each line. Press ENTER at the end of each line.
30
WordPad, Notepad Life table example\1-Example 1d.txt.
NotePadWordPad.
Prepare a data file for TWOSEX-MSChart in folder “Life table example\1-Example 1d”.
Use pure text editors: WordPad, Notepad.
Prepare a data file for TWOSEX-MSChart in folder “Life table example\1-Example 1d”.
WordPad, Notepad.
Note! Use only a pure text editor (e.g., WordPad, Notepad) to write your data file (i.e., txt file). If you face problem with Notepad, you can try WordPad.
Use “.” as decimal symbol Don’t use “,” as decimal symbol
Control panel Clock, Language, and Region Region and language Change keyboards or other input
method Formats Format: English Additional settings Decimal
symbol “.”
Exercise 1: Prepare your data file (20 individuals) (20)
No. Sex Developmental duration Daily fecundity
Egg Larva Pupa Adult 1 2 3 4 5 6 7 8 9
1 M 6 11 12 6
2 F 6 10 11 10 0 124 12 0 4 2
3 F 6 11 10 10 0 22 74 13 0 1
4 F 6 12 10 7 0 97 28 1 4 0
5 M 6 12 11 5
6 F 6 12 11 14 0 61 11 7 15 15 2 3 4
7 M 6 13 10 8
.. .. .. .. .. ..
.. .. .. .. .. ..
20 N 6 -10
Please use Notepad to write a life table data file and save in the folder “Life table examples\1-Life table example”.
"Example of life table raw data" "Chi, H." “25C" 20 3,4 F,Egg,Larva,Pupa,Female M,Egg,Larva,Pupa,Male N,Egg,Larva,Pupa,Unknown Larva, Pupa 1,M,6,11,12,6 2,F,6,10,11,10,0,124,12,0,4,2,-1 3,F,6,11,10,10,0,22,74,13,0,1,-1 4,F,6,12,10,7,0,97,28,1,4,0,-1 5,M,6,12,11,5 6,F,6,12,11,14,0,61,11,7,15,15,2,3,4,-1
7,M,6,13,10,8 8,F,6,11,9,11,0,0,0,26,36,9,6,2,-1 9,F,6,12,11,9,67,37,10,5,1,-1 10,M,6,12,11,6 11,M,6,12,12,7 12,F,6,12,12,8,75,48,12,4,1,-1 13,F,6,13,10,10,2,90,5,12,-1 14,M,6,13,11,8 15,F,6,13,12,10,44,53,11,15,2,-1 16,F,6,13,11,9,100,17,45,8,-1 17,M,6,14,10,6 18,F,6,14,11,6,9,111,27,4,-1 19,N,6,13,-8 20,N,6,-10
Please add the rearing cost to your data file and save in the folder “Life table examples\5-life table with rearing cost”.
"Example of life table raw data" "Chi, H." “25C" 20 3,4 F,Egg,Larva,Pupa,Female M,Egg,Larva,Pupa,Male N,Egg,Larva,Pupa,Unknown 0,1,0,2,1 Larva, Pupa 1,M,6,11,12,6 2,F,6,10,11,10,0,124,12,0,4,2,-1 3,F,6,11,10,10,0,22,74,13,0,1,-1 4,F,6,12,10,7,0,97,28,1,4,0,-1 5,M,6,12,11,5 6,F,6,12,11,14,0,61,11,7,15,15,2,3,4,-1
7,M,6,13,10,8 8,F,6,11,9,11,0,0,0,26,36,9,6,2,-1 9,F,6,12,11,9,67,37,10,5,1,-1 10,M,6,12,11,6 11,M,6,12,12,7 12,F,6,12,12,8,75,48,12,4,1,-1 13,F,6,13,10,10,2,90,5,12,-1 14,M,6,13,11,8 15,F,6,13,12,10,44,53,11,15,2,-1 16,F,6,13,11,9,100,17,45,8,-1 17,M,6,14,10,6 18,F,6,14,11,6,9,111,27,4,-1 19,N,6,13,-8 20,N,6,-10
Rearing cost
A1. Open data file A1.
Open data file
Life table only? ?
If it is a regular life table without rearing cost, you have to select “Yes”. Yes.
Is two-sex mating necessary?
48Copyright 1997-2020 Hsin Chi
Basic analysis
A2. Basic Run
Fecundity coefficient = 1 ( = 1)
In most cases, you click on OK. If you enter 0.5, you can see the effect of 0.5
fecundity on the population parameters.
Default: Time unit = 1 day ( 1)
You should enter the value for the time unit you used in life table study.
Time unit: 1, 0.5,0.3, 0.25, 0.2, 0.16… To make it easier to run the program, I let you enter 0.3 for
8-h data and 0.16 for 4-h data.
Because 8 h is truly 1/3 d, not 0.3 d, the true value 1/3 and 1/6 is used in the calculation in the TWOSEX program. Because most people use 1 d or 0.5 d, you may not notice that special care has been taken into consideration. This is important in scientific calculation.
More options? (Mostly NO. NO)
1. If you want to see more options, choose Yes. 2. In case you face problem, you should try Yes. 3. If you want to run 3D life table, pick Yes.
If there is no error in your data file, but you still cannot run the program, you have to try NO here.
“No”.
Precision level of r (In general, just click on OK)
You can use this button to see the effect of precision level on the estimation of r and λ. It is only for teaching only.
Save bootstrap survival rates of specific age bootstrap
You can use this option to calculate the SE of survival rate (lx) for any specific age.
(lx)(SE).
Find the age for a specific survival rate
You can use this option to find the SE of age that gives a specific survival rate (lx). (lx)SE.
Life table only?
“…_17_Cxj_For_Predation_Prog.txt” from your consumption rate folder.
“No”(cxj)cxj
Quiz ()
?
9997.10 73.126
207.69 7273.126
20 7.69
f
f Nf must be an integer. However, because F is a mean value (it is not an integer) and due to the rounding-off, we get 10.9997. But we know it must be 11.
(integer) F ()
Definition of parameters
Population parameters ()
1 0
1
Net reproductive rate
1
1
1
The total mean number of offspring that an average individual (including females, males, and those died in immature stage) can produce during its lifetime. The magnification that a population will increase after one generation. ( )( )
Chi (1988) proved
N R f0
where Nf is the number of female adults emerged from the total individuals N used at the beginning of life table study and F is the mean fecundity of this Nf females. This relationship is valid for the age-stage, two-sex life table and female age-specific life table. For two-sex life table: N-Nf = Nd +Nm (number of dead in preadult stage + number of male adults). For female life table: N-Nf= Nd (number of dead in preadult).
Intrinsic rate of increase

sfe TWOSEX life table uses this one.
It is the population growth rate as time approaches infinity and population reaches the stable age-stage distribution. The population size will increase at the rate of er per time unit.
Intrinsic rate of increase (time unit 0.5)

TWOSEX life table uses this one.
It is the population growth rate as time approaches infinity and population reaches the stable age-stage distribution. The population size will increase at the rate of er per time unit.
All r and λ are irrational numbers, except for r = 0 and λ = 1.
In general, eq is irrational for any non-zero rational q.
λ = er. For any non-zero r, λ is irrational, thus, its corresponding r is irrational.
Reference:
Aigner, Martin; Ziegler, Günter M. (1998), Proofs from THE BOOK (4th ed.), Berlin, New York: Springer- Verlag, pp. 27–36, doi:10.1007/978-3-642-00856-6, ISBN 978-3-642-00855-9.
Finite rate of increase

TWOSEX life table uses this one.
The finite rate is the population growth rate as time approaches infinity and population reaches the stable age- stage distribution. The population size will increase at the rate of λ per time unit.
Mean generation time
It is the period that a population requires to increase to R0-fold of its size as time approaches infinity and the population settles down to a stable age-stage distribution (SASD), i.e., stable age distribution SAD, too. A shorter generation time doesn’t mean faster growth rate.
r
Mean generation time (T)
The mean generation time (T) is defined as the length of time that a population needs to increase to R0-fold of its size (i.e., erT = R0
or λT = R0) at the stable age distribution (SAD).
Lotka (1913)
Lotka, A. J. 1913. A natural population norm. II. Journal of the Washington Academy of Sciences. Vol. 3, No. 9 (MAY 4, 1913), pp. 289-293.
Lewis (1942)
R
R
R
“A net reproduction rate >≤ 1 means λ >≤ 1, and vice versa, but the magnitudes of the two measures will be different.”
Lewis, E. G. 1942. On the generation and growth of a population. Sankhya 6: 93-96.
Important and basic relationship
R0 > 1, r > 0, λ > 1. R0 = 1, r = 0, λ = 1. R0 < 1, r < 0, λ < 1.
How many digits should you report?
re
Please report at least 3 or 4 decimal points.
Quiz
?
?
Quiz
?
Quiz
Answer Answer
Basic Run completed
“Whether you can observe a thing or not depends on the theory which you
use. It is the theory which decides what can be observed.” – A. Einstein
Age (day)
Su rv
iv al
r at
e (s
20 Egg L1 L2 L3 L4 Pupa Female Male mx
Age (day)
Su rv
iv al
r at
e (l
x)
0
10
20
30
40
lx
mx
If you use a female age-specific life table, you will never see the stage differentiation and male population.
Life table is real big data
Life table data Survival rate
Developmental rate
Life table analysis must be based on theories
Life table theory: 1. Net reproductive rate 2. Intrinsic rate 3. Life expectancy 4. Reproductive value 5. Population projection 6. ……………… Statistical theory: 1. Bootstrap sampling 2. Central limit theory 3. ………………
They are all closely related.
Truth exists independent of awareness, but scientists bring it to awareness
Newton did not invent gravity.
Scientists did not invent the truth.
Scientists discover the truth and derive mathematical equations to describe the truth in formulated theory.
The truth can then be applied to numerous observed data without making any mistake.
Bootstrap
A3. Bootstrap (Estimation of SEs) A3. bootstrap (SEs)
Same bootstrap samples? bootstrap?
If it is the first time you analyze this data file, you have to enter 1.
1. Copyright 1997-2020 Hsin Chi 90
Save all bootstrap table results? (Mostly NO) bootstrap? (No)
If you choose “yes”, you can see all results. But it takes disk space.
Effective bootstrap only? (Mostly yes) bootstrap? (Yes)
In most cases, you choose Yes. If you want to see the non-effective bootstrap, choose No.
Frequency chart (50 or 20 classes)
It is OK to choose Yes or No.
Enter the number of bootstrap
Note well! You have to use B ≥ 100,000 to get stable estimates of population parameters.
Enter the sample size
In most cases, you just click OK. The program will use the number of individuals you used for life table study as sample size.
Do you need life tables of 0.025 and 0.975 percentiles? (They give you the variability of your life table.)
Note well! You have to use B ≥ 100,000 to get reliable and stable percentiles.
Quick sort or selection sort? ?
If you cannot use 128 classes, please use 32 or 16. 128
Do you need bootstrap frequency files and normal curves?
In most cases, you choose No. If you want to prepare figures of the normal curves,
you can choose Yes.
Congratulation!
Life table analysis completed. Don’t forget to read the output file (…._0a_Life table_Output.txt).
Bootstrap completed
Show detail results
Important! ! In the file “_0A_Life table_Output.txt” you can find all your data and analytical results. You must read this file carefully and to find important features of your data and to report them in your paper. You have to interpret your results based on life table theory.
“_0A_Life table_Output.txt” ()
How to read the _output.txt file
To read the Example_output.txt file in aligned column format, you have to select Courier New font. (In general you don’t need to change it).
If the _output.txt is not in aligned format, then change Format – Font – Courier New.
The data in Example_output.txt file contains limited digits. But in graphic text file (e.g., Example_sxj.txt), more digits are listed.
File list 1 Example.txt Example_0A_Life Table_Output.txt Example_0B_Bootstrap_Output.txt Example_0C_Bootstrap_Stage mean_Output.txt Example_0D_Bootstrap_APOP and TPOP_Output.txt Example_0E_Bootstrap_Stage mortality_Output.txt Example_1_Fig_Sxj.txt Example_2_Fig_FxLxMxLxMx.txt Example_3_Fig_Exj.txt Example_4_Fig_Vxj.txt Example_5_Fig_SASD.txt Example_5_Fig_SASD_in_percentage.txt Example_6_Fig_Pxj.txt
Use these files to prepare your figures (SigmaPlot, Origin, Excel, etc).
Use this data for tables.
If you report exj, you have to cite following reference
exj,
Chi, H. and H. Y. Su. 2006. Age-stage, two-sex life tables of Aphidius gifuensis (Ashmead) (Hymenoptera: Braconidae) and its host Myzus persicae (Sulzer) (Homoptera: Aphididae) with mathematical proof of the relationship between female fecundity and the net reproductive rate. Environmental Entomology 35: 10-21.
If you report vxj, you have to cite following references
Tuan, Shu-Jen, Chung-Chieh Lee and Hsin Chi. 2014a. Population and damage projection of Spodoptera litura (F.) on peanuts (Arachis hypogaea L.) under different conditions using the age-stage, two-sex life table. Pest Manag. Sci. 70: 805–813.
Tuan, Shu-Jen, Chung-Chieh Lee and Hsin Chi. 2014b. Erratum: Population and damage projection of Spodoptera litura (F.) on peanuts (Arachis hypogaea L.) under different conditions using the age-stage, two-sex life table. Pest Manag. Sci. 70: 1936.
File list 2 Example_10_SASD and Oviposition age_for_CONSUME.txt Example_11_Bootstrap samples-date_2020912-time_15734.txt Example_15_For_TIMING_1 control.txt Example_15_For_TIMING_2 controls.txt Example_15_For_TIMING_2 Releases.txt Example_15_For_TIMING_3 controls.txt Example_15_For_TIMING_5-harvest.txt Example_16_For_TWOSEX_COMPETITION_program.txt Example_17_For_2-Sex Predation_Predator data.txt Example_18_For_2-Sex Predation_Prey data.txt Example_19_APOP_TPOP_PostOVI for Consume.txt
_11_Bootstrap samples-date_....txt This file contains bootstrap record, you can use this file to run A3 to get the same bootstrap results. bootstrap A3
Use this data for simulation.
File list 3 Example_Effect Boot-A1-R0.txt Example_Effect Boot-A2-R0-with serial nr.txt Example_Effect Boot-A3-r.txt Example_Effect Boot-A4-r-with serial nr.txt Example_Effect Boot-A5-lambda.txt Example_Effect Boot-A6-lambda-with serial nr.txt Example_Effect Boot-A7-T.txt Example_Effect Boot-A8-doubling time.txt Example_Effect Boot-A9-GRR.txt Example_Effect Boot-B1_Total longevity.txt Example_Effect Boot-B2_APOP.txt Example_Effect Boot-B3_TPOP.txt Example_Effect Boot-B5_Fecundity.txt
Use these files for paired test.
Please copy the following files to the respective folder:
…_0Hd_life Table_0.025 lambda-sample_67204.txt …_0Hd_life table_0.975 lambda-sample_12332.txt
\Deaktop\Life table lecture-JAU\4-Life table examples\2-Life table 0.025
\Desktop\Life table lecture-JAU\4-Life table examples\3-Life table 0.975
File list 4 Example_Effect Boot-U1_Age of 0.5 lx.txt Example_Effect Boot-U2_lx-at age 21.txt Example_Effect Boot-V1_Peak Reproductive value.txt Example_Effect Boot-V2_Age of Peak Rep. value.txt Example_Effect Boot-W2_Fecundity (NFr).txt Example_Effect Boot-W2_Oviposition days.txt Example_Effect Boot-W3_Preadult survival.txt Example_Effect Boot-W4_Surv Prob to 1_Egg.txt Example_Effect Boot-W4_Surv Prob to 2_Larva.txt Example_Effect Boot-W4_Surv Prob to 3_Pupa.txt Example_Effect Boot-W4_Surv Prob to 4_Preadult.txt Example_Effect Boot-W4_Surv Prob to 5_Female.txt Example_Effect Boot-W4_Surv Prob to 6_Male.txt Example_Effect Boot-W4_Surv Prob to 7_Adult.txt
File list 5
File list 6 Example_Effect Boot-W8_Nf to adults ratio.txt Example_Effect Boot-W8_Nf to N ratio.txt Example_Effect Boot-W8_Nf.txt Example_Effect Boot-W8_Nfr to N ratio.txt Example_Effect Boot-W8_Nfr to Nf ratio.txt Example_Effect Boot-W8_Nfr.txt Example_Effect Boot-W8_Nm to N ratio.txt Example_Effect Boot-W8_Nm to Nf ratio.txt Example_Effect Boot-W8_Nm.txt Example_Effect Boot-W8_Nn to N ratio.txt Example_Effect Boot-W8_Nn.txt
What is the definition of sex ratio? ?
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File list 8 Example_Effect Boot-Z0_Stage mean_1_Egg.txt Example_Effect Boot-Z0_Stage mean_2_Larva.txt Example_Effect Boot-Z0_Stage mean_3_Pupa.txt Example_Effect Boot-Z0_Stage mean_4_Adult.txt Example_Effect Boot-Z1_Stage mean_Sex_F_1_Egg.txt Example_Effect Boot-Z1_Stage mean_Sex_F_2_Larva.txt Example_Effect Boot-Z1_Stage mean_Sex_F_3_Pupa.txt Example_Effect Boot-Z1_Stage mean_Sex_F_4_F adult.txt Example_Effect Boot-Z2_Stage mean_Sex_M_1_Egg.txt Example_Effect Boot-Z2_Stage mean_Sex_M_2_Larva.txt Example_Effect Boot-Z2_Stage mean_Sex_M_3_Pupa.txt Example_Effect Boot-Z2_Stage mean_Sex_M_4_M adult.txt Example_Effect Boot-Z3_Stage mean_Sex_N_1_Egg.txt Example_Effect Boot-Z3_Stage mean_Sex_N_2_Larva.txt
Note well!
If you are using Turkish, Farsi, Chinese or other operation systems, you might see following problems in the files prepared by TWOSEX: " "Project:Life table of Stethorus reared on the cowbean" "
"Next line is bootstrap sample size (n)"
103
100000
"""i""",103,15.9029126213592
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File list 10 Example_G_APOP and TPOP of female.txt Example_G_APOP.txt Example_G_Egg per day during oviposition period.txt Example_G_Eggs per ovi-day_for general boot.txt Example_G_Eggs per ovi-day_NOT for general boot.txt Example_G_Oviposition days.txt Example_G_Oviposition period.txt Example_G_Post-oviposition period.txt Example_G_total fecundity all females.txt Example_G_total fecundity of rep females.txt Example_G_Total longevity-all.txt Example_G_Total longevity-female.txt Example_G_Total longevity-Male.txt Example_G_Total longevity-N type.txt Example_G_TPOP.txt
File list 11 Example_H_Stage duration-All adult.txt Example_H_Stage duration-Egg.txt Example_H_Stage duration-Female adult.txt Example_H_Stage duration-Larva.txt Example_H_Stage duration-Male adult.txt Example_H_Stage duration-Pupa.txt Example_H_Stage-Begin-End-duration-All adult.txt Example_H_Stage-Begin-End-duration-Egg.txt Example_H_Stage-Begin-End-duration-Female adult.txt Example_H_Stage-Begin-End-duration-Larva.txt Example_H_Stage-Begin-End-duration-Male adult.txt Example_H_Stage-Begin-End-duration-Pupa.txt Example_H_Stage-duration-Larva to Pupa_All sex.txt Example_H_Stage-duration-Larva to Pupa_All sex-row.txt Example_H_Stage-duration-preadult-all sexes.txt Example_H_Stage-duration-preadult-F.txt Example_H_Stage-duration-preadult-M.txt
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File list 13
File list 14
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twosex-mschartcan be used on four ways to run twosex

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