vaporized hydrogen peroxide decontamination of two ... · absa nd52 annual conference, miami,...
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ABSA 52nd Annual Conference, Miami, Florida, October 18‐21, 2009Session XIV: ROUND TABLE: Inactivation and Decontamination
Vaporized Hydrogen Peroxide Decontamination of Two Specialized Animal Transport UnitsTwo Specialized Animal Transport Units
Lesley Homer, RBP, CBSP
Regional Biocontainment Laboratory
f b hUniversity of Pittsburgh
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Animal Transport ComponentsComponents
Mobile transfer cart
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Animal Transport ComponentsComponents
Plastic isolator
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Transport Units:D t i ti N dDecontamination Needs
1. Cart remains in ABSL‐3– Mobile transfer cart– Interior decontamination required prior to transporting a different animal/pathogen
2. Cart comes out of ABSL‐3– Mobile transfer cart
Interior and exterior decontamination required– Interior and exterior decontamination required to remove cart for service and certification
3 Cart comes out of ABSL‐33. Cart comes out of ABSL‐3– Plastic isolator– Interior and exterior decontamination required to release cart for the next transportto release cart for the next transport
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The VHP Process
VHP ports
VHP 100‐P unitVHP sensor 0‐2000 ppm
VHP sensor 0.0‐20.0 ppmBiological & chemical indicators (Geobacillus spores)
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The VHP Process
70
80
450
500
50
60
300
350
400
Aeration
20
30
40
150
200
250 % HumidityVHP, PPM
Decontamination
0
10
0
50
100
0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170
Time in Minutes
Conditioning: T=10 minutes
Condition
Dehumidify
Conditioning: T=10 minutesDecontamination: T=25 minutesAeration: T=55 minutes
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Mobile Transfer Cart: Cart Remains in ABSL 3
• Done between pathogens
Cart Remains in ABSL‐3
• Interior is treated with VHP
• Must clean and surface‐disinfect prior to VHP
• VHP hoses connected directly to cartVHP hoses connected directly to cart
• 30 min VHP + 3 hr aeration
• Reopen valves after cycle; restore fresh air to interior
S d f• Same‐day reuse of cart
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Mobile Transfer Cart: Cart Remains in ABSL‐3Cart Remains in ABSL‐3
Results:R l 3 ti• Ran cycle 3 times
• 29/30 biological indicators failed to grow • Cycle parameters are considered validatedy p• For future cycles, will use 4 BI/CI per cycle
Lessons Learned:Lessons Learned:• Less labor‐intensive• Rapid turnaround time; same‐day to release/reuse equipment• Real‐time [VHP] not yet available• Battery‐operated fan important to distribute VHP• No corrosion or deleterious effect on cart• No corrosion or deleterious effect on cart
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Both Carts:R f ABSL 3
VHP in an effective decontamination room:
Remove from ABSL‐3
– Fill room with VHP and maintain at cidal level
– Prevent leakage of VHP
– No harm to nearby people, animals
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Tape on door from soiled corridor into airlock VHP of Carts in Airlock
FAN
in Airlock
C t204 ft3 Cart204 ft
FAN FAN
Tape on door from suite into airlock
Plastic on door from airlock into suite
Tape on door from suite into airlock
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VHP of Carts in Airlockin Airlock
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VHP of Carts in Airlockin Airlock
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Indicators
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Sequence of Events, Carts in Airlock
Date Procedure Personnel
Day 1 Clean and sanitize cart,move to treatment room.
2 vet techs
Stage cart and airlock with indicators. BSOgTape door and place plastic.
Connect VHP equipment to room. Place fans.Tape last door.
BSO
Adjust supply and exhaust air.Start VHP cycle.Monitor VHP level in animal suite.
Operations ManagerBSO1 vet tech
Complete VHP cycle. Operations ManagerComplete VHP cycle.Begin to adjust supply and exhaust air.
Operations ManagerBSO
Day 2 Retrieve indicators and incubate.Move cart to clean airlock.
BSO or Vet tech
Day 3 Read indicator results.Release cart to clean area.
BSO or Vet tech
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Cycle Parameters:Carts in AirlockCycle Phase Air Flow
SCFMTimeMin.
Injection Rate
Other
Dehumid. 15 0‐10 ‐‐‐‐ Hum = 7.6 mg/L
Condition 15 20 10 g/min ‐‐‐
Decon 15 45 7 g/min ‐‐‐
Aeration 15 60 ‐‐‐ ‐‐‐Aeration 15 60 ‐‐‐ ‐‐‐
Biological Indicator #
Location Day 1 result
Day 7 result
1 Northeast airlock wall near floor Negative Negative2 Southeast corner of airlock behind cage
railingNegative Negative
3 North/Northeast wall near ceiling Negative Negative4 West wall behind cage railing Negative Negative
Placement of BI
4 West wall behind cage railing Negative Negative5 South wall of airlock Negative Negative6 Top of mobile transfer cart on exhaust
filterNegative Negative
7 Inside mobile transfer cart, NW corner Negative Negativenear ceiling
8 Inside MTC, N corner on floor Negative Negative9 Inside MTC, SE corner near ceiling Negative Negative10 Inside MTC, S corner on floor Negative Negative
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Results, Carts in Airlock
• 21 cart treatments done, 5 treatments of other equipment in airlockairlock.
• Pre‐cleaning and sanitation of animal carts must be done.
• Multiple cycles run with 10+ indicators per cycle.p y p y– Carts: 168 total BI, 13 total positive BI at day 7.
• After engineering control incident and corrective actions, the validation was repeated and acceptedvalidation was repeated and accepted.
• “Validation”: – Usually NMT 10% positive BI in triplicate.
– If >10% positive BI, take corrective actions, then repeat validation.
• Post‐validation, require at least 4 BI/CI per cycle.
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Lessons Learned, Carts in Airlock
• Must clean and surface‐disinfect prior to VHP
• Labor‐intensive process requiring 3 days to release equipment
• Airlock is not available for animal transport or waste processing during the treatmentduring the treatment
• Number and position of oscillating fans is key for distributing VHP
• Equipment with doors must be opened and facing fans
• Equipment with motors and fans should be left running to circulate VHP through internal systems
• No corrosion or deleterious effect on carts or other equipment• No corrosion or deleterious effect on carts or other equipment treated including electronics
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Conclusions and Wish List
Conclusions:• VHP treatment can be used to decontaminate animal
transport equipment without damagetransport equipment without damage.
• Interior‐only VHP decon of mobile transfer cart is rapid and less intrusive to research and husbandry.
• Use of soiled ABSL‐3 suite airlocks is necessary forUse of soiled ABSL 3 suite airlocks is necessary for decontamination of plastic cart and other equipment that needs to be released from the ABSL‐3 facility.
Wish list: • Dedicated VHP room; plastic isolator with VHP ports.
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Special thanks to
RBL Management Team:
K ll St f C l Ph D Di tKelly Stefano Cole, Ph.D., Director
Dan Fisher, Operations Manager
Amy Hartman Ph D Research ManagerAmy Hartman, Ph.D., Research Manager
Dennis Heflin, Support Services Manager
Doug Reed, Ph.D., Aerobiology Director
Anita Trichel, D.V.M., Ph.D., Clinical Veterinary Director