white blood cell and lymphocyte subset counts in a follow-up of normal-weight healthy pregnant women
TRANSCRIPT
12-‐20 weeks Recruitment (N=118)
24 weeks (2nd trimester)
(N=118)
34 weeks (3rd trimester)
(N=118)
Delivery (N=65)
F O L L O W – U P S T U D Y
Characteris'cs Normal-‐weight pregnant women (N = 118)
Age (years)1 30.97 ± 4.20
Height (cm)1 1.64 ± 0.06
Weight (kg)1 Before pregnancy Second trimester (24 weeks) Third trimester (34 weeks) At delivery*
58.40 ± 5.66 64.78 ± 7.13 69.54 ± 6.77 70.58 ± 6.42
Weight gain over pregnancy (kg)1* 11.7 ± 1.45
Dura9on of gesta9on (weeks)1 39.5 ± 2.67
BMI (kg/m2)1 Before pregnancy Second trimester (24 weeks) Third trimester (34 weeks) At delivery*
21.98 ± 1.67 24.29 ± 2.40 25.92 ± 2.14 26.59 ± 2.16
1Data expressed as mean ± standard devia9on (S.D.). *Data available from a subsample (N = 65) from 118 pregnant women.
24 weeks 34 weeks Delivery 24 weeks 34 weeks Delivery
*
♯
♯
♯
*
♯
♯
♯
* : P24-‐34 <0,05 ♯: P24-‐parto y P34-‐parto <0,05
* : P24-‐34 < 0,05 ♯: P24-‐delivery and P34-‐delivery < 0,05
Total leukocytes
Neutrophils
Lymphocytes
Monocytes
Total leukocytes-‐C
Neutrophils-‐C
Lymphocytes-‐C
Monocytes-‐C
24 weeks 34 weeks Delivery 24 weeks 34 weeks Delivery
*
♯
♯
♯
*
♯
♯
♯
* : P24-‐34 <0,05 ♯: P24-‐parto y P34-‐parto <0,05
* : P24-‐34 < 0,05 ♯: P24-‐delivery and P34-‐delivery < 0,05
125%
62% 40%
48% 17%
Total leukocytes
Neutrophils
Lymphocytes
Monocytes
Total leukocytes-‐C
Neutrophils-‐C
Lymphocytes-‐C
Monocytes-‐C
24 weeks 34 weeks Delivery 24 weeks 34 weeks Delivery
*
♯
♯
♯
*
♯
♯
♯
* : P24-‐34 <0,05 ♯: P24-‐parto y P34-‐parto <0,05
* : P24-‐34 < 0,05 ♯: P24-‐delivery and P34-‐delivery < 0,05
125%
62% 40%
48% 17%
Total leukocytes
Neutrophils
Lymphocytes
Monocytes
Total leukocytes-‐C
Neutrophils-‐C
Lymphocytes-‐C
Monocytes-‐C
Pregnancy was related to an increase in neutrophils while trying to preserve the absolute numbers of lymphocytes and monocytes. Possible mechanisms to explain our results: • To retain desirable levels of immune system reac9vity. • To reserve energy for pregnancy, as the innate immune system is
less energe9cally costly to maintain than the adap9ve immune system.
Miller. Am J Hum Biol. 2009;21(3):401-‐3.
24 weeks 34 weeks Delivery 24 weeks 34 weeks Delivery
Mature T-‐cells
Helper T-‐cells
Cytotoxic T-‐cells
NK cells
Lymphocytes B
Mature T-‐cells-‐C
Helper T-‐cells-‐C
Cytotoxic T-‐cells-‐C
NK cells-‐C
Lymphocytes B-‐C
* *
*
*
* *
♯
♯
♯
♯
♯
♯
♯
♯
* : P24-‐34 < 0,05 ♯: P24-‐delivery and P34-‐delivery < 0,05
24 weeks 34 weeks Delivery 24 weeks 34 weeks Delivery
Mature T-‐cells
Helper T-‐cells
Cytotoxic T-‐cells
NK cells
Lymphocytes B
Mature T-‐cells-‐C
Helper T-‐cells-‐C
Cytotoxic T-‐cells-‐C
NK cells-‐C
Lymphocytes B-‐C
* *
*
*
* *
♯
♯
♯
♯
♯
♯
♯
♯
* : P24-‐34 < 0,05 ♯: P24-‐delivery and P34-‐delivery < 0,05
10%
13%
20% 25% 50% 40%
24 weeks 34 weeks Delivery 24 weeks 34 weeks Delivery
Mature T-‐cells
Helper T-‐cells
Cytotoxic T-‐cells
NK cells
Lymphocytes B
Mature T-‐cells-‐C
Helper T-‐cells-‐C
Cytotoxic T-‐cells-‐C
NK cells-‐C
Lymphocytes B-‐C
* *
*
*
* *
♯
♯
♯
♯
♯
♯
♯
♯
* : P24-‐34 < 0,05 ♯: P24-‐delivery and P34-‐delivery < 0,05
10%
13%
20% 25% 50% 40%
*Par'al correla'ons adjusted by maternal age.
24 weeks: R = 0,25, P = 0,01 34 weeks: R = 0,26, P = 0,004
24 weeks: R = 0,26, P = 0,003 34 weeks: R = 0,25, P = 0,004
24 weeks: R = 0,20, P = 0,03 34 weeks: R = 0,27, P = 0,002
• There are immunological changes during pregnancy that mediate the immune adapta9on between mother and foetus.
• Pregnancy was related to an increase in innate immune cells
(neutrophils), and a decrease in B-‐cells and NK cells. • The correla9ons observed between preconcep9onal BMI and
lymphocytes during pregnancy suggest that pregnancy does not disrupt the habitual posi9ve rela9onship between nutri9onal status and lymphocyte numbers.
www.proyectopreobe.com
Cris'na Campoy
Ascensión Marcos
Esther Nova
L. Esperanza Díaz
Cris'na Campoy
Ascensión Marcos
Esther Nova
L. Esperanza Díaz
THANK YOU FOR YOUR KIND ATTENTION!