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WRLFMD Quarterly Report January-March 2013 Reference Laboratory Contract Report 4/26/2013 WRLFMD
WRLFMD Quarterly Report January-March 2013 2013
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WRLFMD Quarterly Report January-March 2013 2013
3
Reference Laboratory Contract Report1,2
January-March 2013
Foot-and-Mouth Disease
1N.B. The content of this report is the property of WRLFMD®, The Pirbright Institute. For presentation,
publication or any other public use, please contact Dr. Jef Hammond, The Pirbright Institute,
[email protected]. 2 Copies of all the individual reports cited herein can be obtained from Dr. Jef Hammond, The Pirbright
Institute, [email protected].
WRLFMD Quarterly Report January-March 2013 2013
4
Summary
Bhutan
Eight samples were received. One sample from cattle in Sakteng, Trashigang, RLDC Kanglung, typed
as FMDV O and genotyped as ME-SA/Ind-2001d being most closely related to a virus from Tamil
Nadu, India collected in 2011.
PR China
The following outbreaks of FMD have been reported during the reporting period:
04/01/2013 - FMD type O in pigs in the Economic development zone, Guangyuan, SICHUAN.
18/02/2013 - FMD type A in pigs in Shange town, Maoming, GUANGDONG.
19/02/2013 - FMD type O in cattle in Deji Village, Qushui, Lasha, TIBET.
15/03/2013 - FMD type A in cattle in Chengbei district, Xining, QINGHAI.
Genotyping of the FMD type A viruses by the LVRI revealed the viruses to belong to the ASIA
topotype, Sea-97 lineage. This lineage is endemic in Southeast Asia. These were closely related to the
type A virus from the Russian Federation (see below).
Iran
Twenty-three viruses were isolated from a batch of 27 samples. Five FMD type O viruses belonged to
the ME-SA topotype, PanAsia-2 lineage (with one belonging to the FAR-09 sub-lineage and four to
ANT-10). Eighteen FMD type A viruses all belonged to the ASIA topotype, Iran-05 lineage (with 1,
11 and 6 belonging to the AFG-07, SIS-10 and SIS-12 sub-lineages, respectively).
Pakistan
Thirty-three samples were received in December 2012. Thirty-one FMD viruses were isolated and
genotyped with the following results:
4 - O/ME-SA/PanAsia-2ANT-10
2 - O/ME-SA/PanAsia-2(unnamed)
8 - A/ASIA/Iran-05HER-10
2 - A/ASIA/Iran-05SIS-12
15 - Asia1/ASIA/Sindh-08
Russian Federation
FMD type A outbreaks have been reported in four villages in the ZABAJKAL`SKIJ KRAY region:
08/03/2013 - Molodezhny, Priargounsky raion (cattle & pigs).
25/03/2013 - Village Srednyaya-Borsya, Kalgansky raion (cattle).
28/03/2013 - Village Duroi, Priargunsky raion (cattle).
28/03/2013 - Village Priargunsk, Priargunsky raion (cattle).
Genotyping of the FMD type A viruses by the FGI-ARRIAH revealed the viruses to belong to the
ASIA topotype, Sea-97 lineage. These were closely related to the type A viruses from P.R. China (see
below).
United Arab Emirates
Two virus isolates were received from an outbreak of FMD in Scimitar-horned oryx (Oryx dammah) in
Abu Dhabi, collected on 29th
January 2013. These were typed as FMDV O and genotyped as ME-
SA/PanAsia-2ANT-10
being most closely related to viruses from Iran collected in 2012-2013 (see below).
AFRICA
Tanzania
Thirty-two samples were received and 18 FMD viruses isolated with the following genotyping results:
15 - A/AFRICA/G-I
2 - SAT1/I
1 -SAT2/IV
WRLFMD Quarterly Report January-March 2013 2013
5
SOUTH AMERICA
No new outbreaks of FMD were reported in the region.
Uncharacterised FMD viruses
A number of outbreaks have occurred where samples have not been sent to the WRLFMD®. It is probable
that the countries involved have performed their own genetic characterisation; however, through the
OIE/FAO laboratory network we would also like to encourage the submission of samples (or complete VP1
sequences) to the WRLFMD®.
An up-to-date list and reports of FMD viruses characterised by sequencing can be found at the following
website: http://www.wrlfmd.org/fmd_genotyping/2013.htm.
Results from samples received at WRLFMD® (status of samples being tested) are shown in Table 1 and a
complete list of clinical sample diagnostics made by the WRLFMD® between January and March 2013 is shown
in Annex 1 Table A. A record of all samples received to The Pirbright Institute (January to March 2013) is
shown in Annex 1 Table B.
Table 1: Status of sequencing of samples received by the WRLFMD® from January to March 2013.
Batch Date Recd. Country Serotype
No. of
samples
No. of
sequences Status
WRLFMD/2012/00037* 13/12/2012 Pakistan O 6 6 Completed
A 10 10 Completed
Asia 1 15 15 Completed
WRLFMD/2013/00001 11/01/2013 Bhutan O 1 1 Completed
WRLFMD/2013/00002 11/01/2013 Iran O 5 5 Completed
A 18 18 Completed
WRLFMD/2013/00004 07/03/2013 United Arab Emirates O 2 2 Completed
WRLFMD/2013/00005 13/03/2013 Tanzania A 15 15 Completed
SAT 1 2 2 Completed
SAT 2 1 1 Completed
Total 75 75
*, pending from last Quarterly Report
WRLFMD Quarterly Report January-March 2013 2013
6
Detailed Analysis:
ASIA
Bhutan
WRLFMD/2013/00001
Date received: 11/01/2013
No. of samples: 8
O/ME-SA/Ind-2001d: 1
FMDV-GD: 7
O/BAN/23/2009 (HQ630684)
O/NEP/5/2010
O/NEP/1/2010
O/BHU/19/2009
O/BHU/8/2009
O/BHU/9/2009
O/BHU/10/2009
O/NEP/3/2010
O/BHU/4/2009
O/BAN/26/2009 (HQ630687)
O/BAN/25/2009 (HQ630686)
O/BAN/27/2009 (HQ630688)
O/BAN/11/2009 (HQ630682)
O/BHU/18/2009
O/NEP/6/2010
O/BHU/3/2009
O/BHU/16/2009
O/BAN/2/2009 (HQ630677)
O/BHU/6/2009
O/BHU/2/2009
O/BHU/5/2009
O/NEP/15/2010
O/NEP/16/2010
O/NEP/17/2010
O/BAN/9/2009 (HQ630681)
O/BHU/40/2009
O/BHU/41/2009
O/BHU/7/2009
O/KEKm2709/IND/2009 (JX070614)
O/KAM0410/IND/2010 (JX070618)
O/UPMe1009/IND/2009 (JX070610)
O/BAN/28/2009 (HQ630689)
O/BAN/29/2009 (HQ630690)
O/BAN/30/2009 (HQ630691)
O/BAN/31/2009 (HQ630692)
O/BHU/26/2009
O/BHU/20/2009
O/BHU/23/2009
O/BHU/25/2009
O/NEP/7/2010
O/BAN/1/2009 (HQ630676)
O/NEP/12/2010
O/CHRn0509/IND/2009 (JX070609)
O/NEP/5/2008 (HQ630693)
O/NEP/7/2008
O/NEP/2/2009 (HQ630694)
O/NEP/3/2009
O/BHU/12/2012
O/TNNa1211/IND/2011 (JX070623)
d
c O/UAE/4/2008
a O/KUW/3/97 (DQ164904)
b O/OMN/7/2001 (DQ164941)
Ind-2001
Irn-2001
PanAsia
PanAsia-2
Pak-98
O/IND/53/79 (AF292107)
O1/Manisa/TUR/69 (AY593823)
O/IND/R2/75* (AF204276)
SEA
ME-SA
EA-3
EA-1
WA
EA-4
EA-2
CATHAY
ISA-1
ISA-2
EURO-SA
100
100
100
99
99
100
73
99
79
86
91
90
95
100
72
90
90
100
99
100
76
88
87
85
84
89
86
79
99
0.02
30 km
© Daniel Dalet / d-maps.com
20 mi
O
FMDV-GD
BHU/12/2012
BHU/14/2012
BHU/15/2012
BHU/16/2012
BHU/17/2012
Samdrup
Jongkhar
Trashigang
Samtse
BHU/10/2012
BHU/11/2012
BHU/13/2012
WRLFMD Quarterly Report January-March 2013 2013
7
Iran
WRLFMD/2013/00002
Date received: 11/01/2013
No. of samples: 27
O/ME-SA/: PanAsia-2ANT-10
: 4
O/ME-SA/: PanAsia-2FAR-09
: 1
A/ASIA/Iran-05AFG-07
: 1
A/ASIA/Iran-05SIS-10
: 11
A/ASIA/Iran-05SIS-12
: 6
NVD: 4
200 km
100 mi
© Daniel Dalet / d-maps.com
Tehran
Qom
Markazi
Qazvin
Zanjan
East
Azerbaijan
Kordestan
Hamedan
Kermanshah
Lorestan
Khuzestan
Chaharmahal
and Bakhtiari
Kohgiluyeh and
Boyer-Ahmad
Fars
Hormozgan
Sistan and
Baluchistan
Kerman
Yazd
Esfahan
Semnan
Mazandaran
North
Khorasan
Razavi
Khorasan
South
Khorasan
OANVD
IRN/37/2012
IRN/4/2013
IRN/5/2013
IRN/7/2013IRN/3/2013
IRN/6/2013
IRN/9/2013
IRN/11/2013
IRN/33/2012
IRN/13/2013
IRN/34/2012
IRN/31/2012
IRN/35/2012
IRN/2/2013
IRN/8/2013
IRN/28/2012
IRN/29/2012
IRN/26/2012
IRN/27/2012
IRN/24/2012
IRN/25/2012
IRN/1/2013
IRN/10/2013
IRN/32/2012
IRN/12/2013
IRN/36/2012
IRN/30/2012
O/IRN/88/2009
O/IRN/113/2010
O/IRN/65/2010
O/IRN/38/2010
O/IRN/20/2010
O/IRN/142/2010
O/IRN/37/2010
O/IRN/19/2010
O/IRN/59/2010
O/IRN/100/2010
O/IRN/33/2010
O/IRN/39/2010
O/IRN/86/2010
O/AFG/190/2010
O/IRN/89/2010
O/IRN/101/2010
O/IRN/34/2010
O/IRN/137/2010
O/IRN/43/2010
O/IRN/13/2010
O/IRN/55/2010
O/IRN/4/2010
O/IRN/30/2010
O/PAK/3/2010
O/PAK/4/2010
O/IRN/2/2010
O/IRN/11/2010
O/PAK/1/2010
O/PAK/2/2010
O/PAK/5/2010
O/PAK/8/2010
O/AFG/242/2010
O/IRN/139/2010
O/TUR/32/2010
O/IRN/35/2010
O/IRN/2/2011
O/IRN/14/2011
O/IRN/36/2012
O/IRN/8/2013
O/IRN/13/2012
O/IRN/9/2013
O/IRN/11/2013
ANT-10
O/IRN/8/2005
PUN-10
BAL-09
O/IRN/87/2009
O/IRN/42/2009
O/IRN/76/2009
O/IRN 3917/2012*
O/IRN 12463/2012*
O/SAU/1/2009
O/SAU/2/2009
O/IRN/41/2009
O/IRN/68/2009
O/IRN/61/2009
O/IRN/80/2009
O/IRN/2/2013
O/IRN/s-005/2013 (IVO)
O/IRN/s-003/2013 (IVO)
O/IRN/21/2012
O/IRN/71/2011
O/IRN/77/2011
O/IRN/62/2011
O/IRN/56/2009
O/IRN/31/2009
O/IRN/60/2009
O/IRN/52/2009
O/IRN/79/2009
O/IRN/83/2009
O/IRN/49/2009
O/IRN/84/2009
O/IRN/43/2009
O/IRN/51/2009
O/IRN/75/2009
O/IRN/34/2009
O/IRN/66/2009
FAR-09
TER-08
SAN-09
PanAsia-2
PanAsia
Irn-2001
Ind-2001
Pak-98 O/IND/53/79 (AF292107)
O/IND/R2/75* (AF204276)
O1/Manisa/TUR/69 (AY593823)
ME-SA
SEA
EA-2
EA-3
EA-4
WA
EA-1
CATHAY
ISA-1
ISA-2
EURO-SA
99
99
99
99
99
99
99
70
73
79
93
86
91
78
85
95
99
99
98
92
99
99
87
99
0.02
A/TUR/13/2011
A/TUR/41/2011
A/TUR/21/2011
A/TUR/5/2011
A/TUR/11/2011
A/TUR/10/2011
A/TUR/23/2011
A/TUR/18/2011
A/TUR/29/2011
A/TUR/16/2011
A/TUR/63/2011
A/IRN/30/2011
A/TUR/22/2011
A/IRN/18/2011
A/IRN/4/2011
A/IRN/5/2011
A/PAK/37/2010
A/IRN/125/2010
A/AFG/125/2010
A/AFG/128/2010
A/AFG/140/2010
A/AFG/163/2010
A/IRN/24/2011
A/IRN/52/2011
A/IRN/20/2011
A/TUR/14/2011
A/IRN/68/2011
A/IRN/69/2011
A/IRN/65/2011
A/IRN/4/2013
A/IRN/s-001/2013 (IVO)
A/IRN/37/2012
A/IRN/22/2012
A/IRN/32/2012
A/IRN/6/2013
A/IRN/3/2013
A/IRN/1/2013
A/IRN/10/2013
A/IRN/24/2012
A/IRN/5/2013
A/IRN/7/2013
A/IRN/Gods/2012*
A/IRN/s-001/2012 (IVO)
A/IRN/s-002/2012 (IVO)
A/IRN/s-003/2012 (IVO)
A/IRN/s-004/2012 (IVO)
A/IRN/31/2012
SIS-10
A/IRN/4/2008 (FJ755067)
A/IRN/34/2012AFG-07
AFG-07
BAR-08
HER-10
A/IRN/33/2012
A/IRN/30/2012
A/IRN/18/2012
A/IRN/17/2012
A/IRN/25/2012
A/IRN/15/2012
A/IRN/16/2012
A/IRN/20/2012
A/PAK/38/2012
A/IRN/35/2012
A/PAK/39/2012
A/IRN/s-002/2013 (IVO)
A/IRN/29/2012
A/IRN/28/2012
SIS-12
FAR-09
ESF-10
A/PAK/5/2006 (FJ755084)
A/PAK/5/2006 (EF494488)
ARD-07
A/IRN/1/2005 (EF208769)
EZM-07
QAZ-11
Iran-05
Iran-99
Iran-87
A22
Thai-87
Sea-97
Iran-96
A15
ASIA
EURO-SA
AFRICA
99
99
98
10092
98
94
96
100
91
99
99
100
83
96
100
100
85
0.02
WRLFMD Quarterly Report January-March 2013 2013
8
Pakistan
WRLFMD/2012/00037
Date received: 13/12/2012
No. of samples: 33
O/ME-SA/PanAsia-2ANT-10
: 4
O/ME-SA/PanAsia-2(unnamed)
: 2
A/ASIA/Iran-05HER-10
: 8
A/ASIA/Iran-05SIS-12
: 2
Asia1/ASIA/Sindh-08: 15
FMDV-GD: 1
NVD: 1
O/PAK/102/2010
O/PAK/98/2010
O/PAK/89/2010
O/PAK/88/2010
O/PAK/80/2010
O/PAK/79/2010
O/PAK/78/2010
O/PAK/77/2010
O/PAK/112/2010
O/AFG/253/2010
O/PAK/74/2011
O/AFG/235/2010
O/AFG/244/2010
O/PAK/63/2011
O/PAK/17/2012
O/PAK/12/2012
O/PAK/20/2012
O/PAK/23/2012
O/IRN/10/2011
O/IRN/35/2011
O/AFG/35/2011
O/AFG/19/2011
O/AFG/259/2010
O/AFG/256/2010
O/AFG/174/2010
O/AFG/178/2010
O/AFG/207/2010
O/AFG/212/2010
O/AFG/211/2010
O/AFG/210/2010
O/AFG/213/2010
O/AFG/214/2010
O/AFG/170/2010
O/PAK/74/2010
O/IRN/78/2010
O/IRN/93/2010
O/IRN/44/2010
O/IRN/55/2010
O/IRN/156/2010
O/AFG/171/2010
O/AFG/196/2010
O/IRN/160/2010
O/IRN/100/2010
O/IRN/101/2010
O/IRN/142/2010
O/IRN/20/2010
O/IRN/33/2010
O/IRN/88/2009
O/IRN/34/2010
O/AFG/217/2010
O/AFG/219/2010
O/AFG/221/2010
ANT-10
O/IRN/8/2005
TER-08 O/TUR/257/2008* (FMDI)
SAN-09 O/TUR/264/2009* (FMDI)
PUN-10 O/PAK/16/2010
FAR-09 O/IRN/31/2009
BAL-09 O/IRN/18/2010
O/PAK/40/2012
O/PAK/41/2012
PanAsia-2
PanAsia O/UKG/35/2001 (AJ539141)
Irn-2001 O/IRN/61/2001 (DQ164896)
Ind-2001
Pak-98 O/PAK/16/2003 (DQ165068)
O/IND/53/79 (AF292107)
O/IND/R2/75* (AF204276)
O1/Manisa/TUR/69 (AY593823)
ME-SA
SEA
EA-2
EA-3
EA-4
WA
EA-1
CATHAY
ISA-1
ISA-2
EURO-SA
99
99
99
99
99
99
99
81
76
71
71
95
89
92
99
81
83
99
75
71
92
88
95
77
99
91
74
86
0.02
200 km
© Daniel Dalet / d-maps.com
100 mi
Balochistan
Khyber Pakhtunkhwa
Province
Punjab
Sindh
Federally Administered
Tribal Areas
Azad Jammu
and Kashmir
Gilgit-BaltistanO
A
Asia 1
FMDV-GD
NVDPAK/29/2012
PAK/10/2012
PAK/13/2012
PAK/39/2012
PAK/9/2012
PAK/16/2012
PAK/11/2012
PAK/12/2012
PAK/32/2012
PAK/33/2012
PAK/34/2012
PAK/38/2012
PAK/18/2012
PAK/19/2012
PAK/21/2012
PAK/22/2012
PAK/24/2012
PAK/25/2012
PAK/26/2012
PAK/27/2012
PAK/30/2012
PAK/35/2012
PAK/36/2012
PAK/37/2012
PAK/31/2012
PAK/23/2012
PAK/40/2012
PAK/41/2012
PAK/14/2012
PAK/15/2012
PAK/17/2012
PAK/20/2012
PAK/28/2012
WRLFMD Quarterly Report January-March 2013 2013
9
A/PAK/13/2012
A/PAK/34/2012
A/PAK/16/2012
A/PAK/33/2012
A/PAK/14/2012
A/PAK/15/2012
A/PAK/32/2012
A/PAK/6/2012
A/PAK/7/2012
A/PAK/8/2012
A/PAK/9/2012
A/AFG/69/2011
A/AFG/71/2011
A/AFG/74/2011
A/AFG/75/2011
A/BAR/18/2011
A/Mainkum/KAZ/02/2012* (JQ765588)
A/IRN/134/2010
A/IRN/7/2011
A/IRN/37/2011
A/AFG/34/2011
A/PAK/86/2011
A/AFG/20/2011
A/AFG/22/2011
A/AFG/24/2011
A/AFG/25/2011
A/AFG/26/2011
A/AFG/27/2011
A/AFG/29/2011
A/AFG/57/2011
A/IRN/32/2011
A/AFG/10/2010
HER-10
A/PAK/39/2012
A/IRN/15/2012
A/IRN/16/2012
A/IRN/20/2012
A/PAK/38/2012
A/IRN/s-002/2013 (IVO)
A/IRN/17/2012
A/IRN/18/2012
SIS-12
A/AFG/6/2007
A/AFG/44/2007
A/IRN/9/2009
AFG-07
A/AFG/16/2009
A/AFG/17/2009
A/AFG/18/2010
A/PAK/24/2010
A/AFG/17/2010
A/AFG/16/2010
A/AFG/15/2010
A/AFG/41/2009
A/PAK/12/2010
A/PAK/23/2010
A/AFG/232/2010
A/AFG/3/2010
A/PAK/1/2009
A/PAK/2/2009
A/PAK/24/2009
A/PAK/31/2009
A/PAK/13/2008
A/AFG/8/2009
A/AFG/13/2009
A/AFG/14/2009
A/AFG/15/2009
AFG-07
FAR-09 A/IRN/78/2009
BAR-08 A/BAR/6/2008
SIS-10 A/IRN/125/2010
ESF-10 A/IRN/9/2010
ARD-07 A/TUR/1/2008
A/IRN/1/2005 (EF208769)
EZM-07 A/TUR/33/2008
QAZ-11 A/IRN/9/2011
Iran-05
Thai-87 A/TAI/118/87* (EF208777)
A/TAI/7/2003 (HQ116312)
A/TAI/2/97 (EF208778)Sea-97
Iran-99 A/IRN/22/99 (EF208772)
Iran-96 A/IRN/1/96 (EF208771)
A23 A23/Kitale/KEN/64 (AY593766)
Iran-87 A/IRN/2/87 (EF208770)
A22 A22/IRQ/64 (AY593763)
A15 A15/Bangkok/TAI/60 (AY593755)
ASIA
A11/GER/29 (AGB)(EU553852)
EURO-SA
AFRICA
98
98
97
100
94
90
77
100
99
71
100
98
98
9989
99
98
89
91
78
95
97
90
85
100
0.02
Asia1/PAK/68/2011
Asia1/PAK/69/2011
Asia1/PAK/67/2011
Asia1/PAK/64/2011
Asia1/PAK/61/2011
Asia1/PAK/60/2011
Asia1/PAK/58/2011
Asia1/PAK/57/2011
Asia1/PAK/56/2011
Asia1/PAK/55/2011
Asia1/PAK/54/2011
Asia1/PAK/53/2011
Asia1/PAK/52/2011
Asia1/PAK/51/2011
Asia1/PAK/50/2011
Asia1/PAK/49/2011
Asia1/PAK/39/2011
Asia1/PAK/37/2011
Asia1/PAK/36/2011
Asia1/PAK/35/2011
Asia1/PAK/33/2011
Asia1/PAK/16/2011
Asia1/PAK/15/2011
Asia1/PAK/14/2011
Asia1/PAK/13/2011
Asia1/PAK/12/2011
Asia1/PAK/11/2011
Asia1/PAK/8/2011
Asia1/PAK/7/2011
Asia1/PAK/111/2010
Asia1/PAK/110/2010
Asia1/PAK/108/2010
Asia1/PAK/106/2010
Asia1/IRN/49/2011
Asia1/AFG/40/2011
Asia1/AFG/66/2011
Asia1/AFG/68/2011
Asia1/AFG/73/2011
Asia1/AFG/78/2011
Asia1/IRN/33/2011
Asia1/IRN/46/2011
Asia1/IRN/43/2011
Asia1/IRN/38/2011
Asia1/IRN/39/2011
Asia1/PAK/6/2011
Asia1/PAK/47/2011
Asia1/PAK/66/2011
Asia1/PAK/34/2011
Asia1/PAK/2/2012
Asia1/PAK/3/2012
Asia1/PAK/1/2012
Asia1/PAK/11/2012
Asia1/PAK/36/2012
Asia1/PAK/37/2012
Asia1/PAK/10/2012
Asia1/PAK/22/2012
Asia1/PAK/27/2012
Asia1/PAK/35/2012
Asia1/PAK/18/2012
Asia1/PAK/19/2012
Asia1/PAK/29/2012
Asia1/PAK/21/2012
Asia1/PAK/24/2012
Asia1/PAK/25/2012
Asia1/PAK/26/2012
Asia1/PAK/30/2012
Asia1/PAK/4/2012
Asia1/PAK/5/2012
Asia1/PAK/8/2008
Sindh-08
Group I Asia1/AFG/1/2001 (DQ121109)
Asia1/HKN/19/74
Asia1/Shamir/ISR/89
Group III Asia1/IND/762/2003* (DQ101240)
Asia1/IND/14/95* (AF390678)
Group VIb Asia1/IRN/10/2004 (DQ121119)
Asia1/YNBS/CHA/58 (AY390432)
Asia1/IND/63/72* (AY304994)
Group V Asia1/IND/18/80 (DQ121116)
Asia1/PAK/1/54 (AY593795)
99
79
99
99
99
99
98
98
99
87
98
97
93
87
96
83
84
0.02
WRLFMD Quarterly Report January-March 2013 2013
10
United Arab Emirates
WRLFMD/2013/00004
Date received: 07/03/2013
No. of samples: 2
O/ME-SA/PanAsia-2ANT-10
: 2
O/UAE/2/2010
O/UAE/3/2010
O/UAE/4/2010
O/UAE/1/2010
O/IRN/160/2010
O/IRN/55/2010
O/IRN/93/2010
O/PAK/7/2010
O/PAK/14/2010
O/IRN/137/2010
O/IRN/88/2009
O/IRN/113/2010
O/IRN/85/2010
O/IRN/100/2010
O/IRN/101/2010
O/IRN/142/2010
O/IRN/20/2010
O/IRN/13/2010
O/IRN/4/2010
O/IRN/30/2010
O/PAK/3/2010
O/PAK/4/2010
O/IRN/2/2010
O/IRN/11/2010
O/AFG/242/2010
O/PAK/1/2010
O/PAK/2/2010
O/PAK/5/2010
O/PAK/8/2010
O/IRN/144/2010
O/IRN/28/2010
O/IRN/88/2010
O/IRN/139/2010
O/TUR/32/2010
O/IRN/83/2010
O/UAE/1/2011
O/IRN/209/2010
O/AFG/110/2010
O/IRN/35/2010
O/IRN/183/2010
O/AFG/160/2010b
O/AFG/193/2010b
O/IRN/96/2010
O/AFG/89/2010
O/AFG/32/2010
O/AFG/59/2010
O/AFG/34/2010
O/AFG/61/2010
O/IRN/2/2011
O/IRN/14/2011
O/UAE/1/2013
O/UAE/2/2013
O/IRN/36/2012
O/IRN/8/2013
O/IRN/13/2012
O/IRN/9/2013
O/IRN/11/2013
ANT-10
O/UAE/1/2007
O/UAE/2/2007
FAR-09 O/IRN/31/2009
BAL-09 O/IRN/18/2010
PUN-10 O/PAK/16/2010
O/IRN/8/2005
TER-08 O/TUR/257/2008* (FMDI)
SAN-09 O/TUR/264/2009* (FMDI)
PanAsia-2
PanAsia
Irn-2001
Ind-2001
Pak-98
O/IND/53/79 (AF292107)
O/IND/R2/75* (AF204276)
O1/Manisa/TUR/69 (AY593823)
ME-SA
SEA
EA-2
EA-3
EA-4
WA
EA-1
CATHAY
ISA-1
ISA-2
EURO-SA
100
100
100
100100
99
100
73
76
72
95
90
91
80
85
99
78
100
99
95
100
82
99
95
71
83
81
99
71
0.02
50 km
© Daniel Dalet / d-maps.com
30 mi
Abu Dhabi
Ajman
Dubai
Fujairah
Ras al-Khaimah
Sharjah
Umm al-Quwain
UAE/1/2013
UAE/2/2013
O
WRLFMD Quarterly Report January-March 2013 2013
11
VP1 sequences received
P.R. China
(LVRI)
Date received: 07/03/2013
A/GDMM-China-2013-S
Date received: 09/04/2013
A/QHXN-CHA-2013-B
Russian Federation
(FGI-ARRIAH)
Date received: 21/03/2013
A/Zabaikalsky/RUS/2013
Topotype: ASIA
Lineage: Sea-97
A/VIT/19/2012
A/VIT/20/2012
A/VIT/18/2012
A/VIT/17/2012
A/VIT/16/2012
A/VIT/15/2012
A/MAY/1/2012
A/TAI/15/2011
A/TAI/24/2011
A/TAI/18/2011
A/TAI/12/2011
A/TAI/17/2011
A/MAY/19/2011
A/MAY/3/2012
A/MAY/4/2012
A/TAI/1/2012
A/TAI/2/2012
A/TAI/13/2011
A/TAI/6/2011
A/TAI/8/2011
A/TAI/9/2011
A/TAI/23/2011
A/TAI/11/2011
A/MAY/2/2011
A/TAI/10/2011
A/TAI/19/2011
A/TAI/20/2011
A/TAI/7/2010
A/THA 67-3/10* (TRRL)
A/THA 66-1/10* (TRRL)
A/TAI/3/2011
A/TAI/16/2011
A/TAI/1/2011
A/TAI/8/2010
A/THA 69-6/10* (TRRL)
A/MAY/17/2011
A/MAY/4/2011
A/MAY/5/2012
A/MAY/20/2011
A/TAI/9/2012
A/TAI/5/2012
A/TAI/6/2012
A/TAI/8/2012
A/TAI/3/2012
A/TAI/4/2012
A/TAI 3/12*
A/TAI 4/12*
A/TAI/7/2012
A/TAI/12/2012
A/QHXN-CHA-2013-B (LVRI)
A/GDMM-CHA-2013-S (LVRI)
A/Zabaikalsky/RUS/2013 (ARRIAH)
A/TAI/7/2003 (HQ116312)
A/HuBWH/CHA/2009 (JF792355)
A/SKR/2/2010 (JQ070331)
A/Pochun/KOR/2010* (GU441855)
A/TAI/2/97 (EF208778)
Sea-97
Thai-87
Iran-87
A22
Iran-96
Iran-99
Iran-05
A15
ASIA
EURO-SA
AFRICA
100
100
80
78
100
100
99
98
96
99
88
99
99
99
99
95
91
90
100
73
79
70
81
0.02
WRLFMD Quarterly Report January-March 2013 2013
12
AFRICA
Tanzania WRLFMD/2013/00005
Date received: 13/03/2013
No. of samples: 32
A/AFRICA/G-I: 15
SAT1/I: 2
SAT2/IV: 1
FMDV-GD: 9
NVD: 5
A/TAN/2/2013
A/TAN/3/2013
A/TAN/61/2012
A/TAN/56/2012
A/TAN/65/2012
A/TAN/66/2012
A/TAN/1/2013
A/TAN/60/2012
A/TAN/70/2012
A/TAN/71/2012
A/TAN/67/2012
A/TAN/68/2012
A/TAN/73/2012
A/TAN/69/2012
A/TAN/72/2012
A/TAN-CVL-2011-0160
A/TAN-CVL-2011-0125
A/TAN-CVL-2011-0066
A/TAN-CVL-2011-0289
A/TAN-CVL-2011-0293
A/COD/2/2011
A/COD/3/2011
A/COD/12/2011
A/COD/11/2011
A/COD/10/2011
A/COD/9/2011
A/COD/8/2011
A/TAN/40/2012
A/TAN/41/2012
A/KEN/22/2009
A/TAN/42/2009
A/TAN/47/2009
A/KEN/28/2008
A/TAN/4/2009
A/TAN/45/2009
A/TAN/11/2009
A/TAN-CVL-2009-0052
A/KEN/12/2005 (K44/2005)
A/KEN/3/2006
A/KEN/7/2008
A/KEN/8/2008
A/TAN/9/2009
A/TAN/11/2008
A/TAN/12/2008
A/TAN-CVL-2008-0155
A/BUN/4/90
A/K49/84*
A/MAL/5/81
A/UGA/1/2002
A/BUN/2/80
A/ZAM/90
A/KEN/42/66 (K18/66)
A/K16/74*
A/K179/71* (KEN/1/76)
A/TAN/4/80
A/TAN/2/68 (T626/68)
A/TAN/3/68 (T809/67)
G-I
G-V
G-IV
G-VII
G-VI
G-II
G-III
AFRICA
EURO-SA
ASIA
9 9
9 9
9 9
9 9
9 0
8 8
8 4
7 0
7 0
7 1
7 2
9 9
9 8
9 9
8 6
9 9
7 6
9 9
9 8
7 1
8 0
7 0
9 8
9 3
7 0
9 8
8 8
9 9
9 9
9 9
7 0
0.02
200 km
100 mi
© Daniel Dalet / d-maps.com
Arusha
Dar es Salaam
Dodoma
Iringa
Kagera
Kigoma
Kilimanjaro
Lindi
Manyara
Mara
Mwanza
Tabora
Shinyanga
Rukwa
Mbeya
Ruvuma Mtwara
Morogoro
Singida
Pwani
TangaPemba North
Pemba South
Zanzibar Central/South
Zanzibar NorthZanzibar Urban/West
A
SAT 1
SAT 2
FMDV-GD
NVD
TAN/54/2012
TAN/53/2012
TAN/56/2012
TAN/60/2012
TAN/61/2012
TAN/65/2012
TAN/66/2012
TAN/67/2012
TAN/68/2012
TAN/69/2012
TAN/70/2012
TAN/71/2012
TAN/72/2012
TAN/73/2012
TAN/1/2013
TAN/2/2013
TAN/3/2013
TAN/55/2012
TAN/57/2012
TAN/58/2012
TAN/59/2012
TAN/62/2012
TAN/63/2012
TAN/4/2013
TAN/74/2012
TAN/64/2012
TAN/51/2012
TAN/47/2012
TAN/48/2012
TAN/52/2012
TAN/49/2012
TAN/50/2012
WRLFMD Quarterly Report January-March 2013 2013
13
SAT1/TAN/22/2012
SAT1/TAN/23/2012
SAT1/TAN/49/2012
SAT1/TAN/27/2012
SAT1/TAN/50/2012
SAT1/TAN/25/2012
SAT1/TAN-CVL-2012-0355
SAT1/TAN-CVL-2012-0354
SAT1/TAN-CVL-2012-0359
SAT1/TAN-CVL-2012-0360
SAT1/TAN-CVL-2012-0361
SAT1/TAN/44/2012
SAT1/TAN/45/2012
SAT1/TAN/11/2012
SAT1/TAN/12/2012
SAT1/KEN/35/2008
SAT1/KEN/11/2005
SAT1/K96/99* (HQ267523)
SAT1/K114/99* (HQ267524)
SAT1/TAN/51/99
SAT1/TAN/51/99 (AY442004)
SAT1/TAN/21/99
SAT1/TAN/19/99
SAT1/TAN/25/99
SAT1/TAN/26/99
SAT1/TAN/60/99
SAT1/KEN/109/2010
SAT1/KEN/110/2010
SAT1/KEN/12/2010
SAT1/KEN/13/2010
SAT1/KEN/107/2010
SAT1/KEN/76/2010
SAT1/KEN/72/2010
SAT1/KEN/104/2010
SAT1/KEN/105/2010
SAT1/KEN/106/2010
SAT1/KEN/90/2010
SAT1/KEN/77/2010
SAT1/KEN/80/2010
SAT1/KEN/88/2010
SAT1/KEN/94/2010
SAT1/KEN/1/2010
SAT1/KEN/4/2010
SAT1/KEN/5/2010
SAT1/KEN/6/2010
SAT1/KEN/8/2010
SAT1/KEN/10/2010
SAT1/T155/71
SAT1/ZIM/23/2003
I (NWZ)
II (SEZ)
IV (EA-1) VIII (EA-3)
III (WZ)
IX V
VII (EA-2)
VI9 9
9 3
9 9
8 7
9 9
9 9
8 6
9 3
8 6
9 9
7 4
9 8
7 0
9 5
9 3
9 9
8 2
9 9
8 5
9 9
8 3
9 9
9 6
9 5
8 6
7 7
9 9
0.05
SAT2/TAN/10/2012
SAT2/TAN/32/2012
SAT2/TAN/9/2012
SAT2/TAN/5/2012
SAT2/TAN/15/2012
SAT2/TAN/16/2012
SAT2/TAN/13/2012
SAT2/TAN/14/2012
SAT2/TAN/34/2012
SAT2/TAN/1/2012
SAT2/TAN/3/2012
SAT2/TAN/37/2012
SAT2/TAN/36/2012
SAT2/TAN/64/2012
SAT2/TAN/7/2012
SAT2/ZAM/1/2012
SAT2/ZAM/2/2012
SAT2/TAN/3/2011
SAT2/TAN/4/2011
SAT2/KEN/18/2011
SAT2/TAN/6/2011
SAT2/KEN/21/2011
SAT2/KEN/20/2011
SAT2/KEN/17/2011
SAT2/KEN/19/2011
SAT2/KEN/12/2011
SAT2/TAN/7/2011
SAT2/TAN/8/2011
SAT2/TAN/18/2012
SAT2/TAN/19/2012
SAT2/TAN/43/2009
SAT2/TAN/4/2004
SAT2/TAN/9/2004
SAT2/K13/87 (HM623687)
SAT2/TAN/21/2004
SAT2/KEN/6/2004
SAT2/KEN/17/2004
SAT2/KEN/8/2005
SAT2/KEN/18/2008
SAT2/KEN/21/2008
SAT2/KEN/19/2008
SAT2/K17/07 (HM623705)
SAT2/K20/07 (HM623706)
SAT2/KEN/13/2007
SAT2/K59/07 (HM623708)
SAT2/KEN/22/2007
SAT2/KEN/14/2007
SAT2/K12/07 (HM623703)
SAT2/KEN/5/2007
SAT2/KEN/10/2007
SAT2/KEN/11/2007
SAT2/ETH/1/90 (1989)(AY343935)
SAT2/KEN/1/84 (K7/84) (AY344505)
IV
III
I
XI
II
V
VI
XIII
XIV
VII
VIII
X
XII
IX
9 9
9 9
9 9
9 9
8 4
9 8
7 2
7 4
8 1
9 7
7 6
9 9
8 2
8 7
8 7
9 5
9 0
8 3
9 9
7 1
9 2
9 8
8 8
9 9
7 3
9 9
8 0
9 9
9 8
9 8
9 3
8 7
9 9
7 5
7 0
0.05
WRLFMD Quarterly Report January-March 2013 2013
14
Vaccine matching
Three FMDV type O isolates (See Table C, Type O for details) from Sri Lanka collected in 2009, 2010 and
2012 were analysed antigenically by the two dimensional virus neutralisation test (2dmVNT). All isolates
showed antigenic matching with O 4625, and O TUR 5/09 and 2 isolates showed matching with O Manisa
(Table C).
Six FMDV type A viruses (See Table C, Type A for details) from Pakistan, Tanzania and Vietnam collected
during 2012 were analysed antigenically by the two dimensional virus neutralisation test (2dmVNT).
Three isolates from Pakistan showed variable antigenic matching with A IRN 2005, A TUR 06 and A22 IRQ
vaccine strains.
The virus from Tanzania showed close antigenic match with A IRN 2005, A TUR 06 and A22 IRQ. The two
isolates form Vietnam showed no match to any of the vaccine strains tested (Table C).
One FMDV type SAT 2 virus (see table C, Type SAT 2 for details) from Egypt 2012 was antigenically matched
with both SAT 2 Eritrea and SAT 2 ZIM vaccine strains, however, the SAT 2 virus collected from Tanzania in
2012 showed no match with either of the tested vaccine strains (Table C).
Annex 1. TABLE A: Clinical sample diagnostics made by the WRLFMD® between January-March 2013
Country WRL for FMD Animal
Date of
Collection Results
Sample
Identification
VI/ELISA RT-PCR Final report
BHUTAN BHU 10/2012 BOVINE 13-Sep-12 NEG POS FMDV GD
BHU 11/2012 BOVINE 24-Oct-12 NEG POS FMDV GD
BHU 12/2012 BOVINE 24-Oct-12 O POS O
BHU 13/2012 BOVINE 24-Oct-12 NEG POS FMDV GD
BHU 14/2012 BOVINE 18-Nov-12 NT POS FMDV GD
BHU 15/2012 BOVINE 18-Nov-12 NT POS FMDV GD
BHU 16/2012 BOVINE 18-Nov-12 NT POS FMDV GD
BHU 17/2012 BOVINE 18-Nov-12 NT POS FMDV GD
IRAN IRN 24/2012 CATTLE 09-Oct-12 A POS A
IRN 25/2012 CATTLE 25-Oct-12 A POS A
IRN 26/2012 SHEEP 30-Oct-12 NEG NEG NVD
IRN 27/2012 SHEEP 30-Oct-12 NEG NEG NVD
IRN 28/2012 CATTLE 04-Nov-12 A POS A
IRN 29/2012 CATTLE 04-Nov-12 A POS A
IRN 30/2012 CATTLE 05-Nov-12 A POS A
IRN 31/2012 CATTLE 06-Nov-12 A POS A
IRN 32/2012 CATTLE 08-Nov-12 A POS A
IRN 33/2012 CATTLE 17-Nov-12 A POS A
IRN 34/2012 CATTLE 17-Nov-12 A POS A
IRN 35/2012 CATTLE 18-Dec-12 A POS A
IRN 36/2012 CATTLE 20-Dec-12 O POS O
IRN 37/2012 CATTLE 24-Dec-12 A POS A
IRN 1/2013 CATTLE 01-Jan-13 A POS A
IRN 2/2013 CATTLE 01-Jan-13 O POS O
IRN 3/2013 CATTLE 01-Jan-13 A POS A
IRN 4/2013 CATTLE 01-Jan-13 A POS A
IRN 5/2013 CATTLE 01-Jan-13 A POS A
IRN 6/2013 CATTLE 01-Jan-13 A POS A
IRN 7/2013 CATTLE 01-Jan-13 A POS A
WRLFMD Quarterly Report January-March 2013 2013
15
IRN 8/2013 CATTLE 01-Jan-13 O POS O
IRN 9/2013 CATTLE 01-Jan-13 O POS O
IRN 10/2013 CATTLE 01-Jan-13 A POS A
IRN 11/2013 CATTLE 01-Jan-13 O POS O
IRN 12/2013 SHEEP 01-Jan-13 NEG NEG NVD
IRN 13/2013 SHEEP 01-Jan-13 NEG NEG NVD
MAURITANIA MAU 1/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 2/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 3/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 4/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 5/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 6/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 7/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 8/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 9/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 10/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 11/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 12/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 13/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 14/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 15/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 16/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 17/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 18/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 19/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 20/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 21/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 22/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 23/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 24/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 25/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 26/2012 CATTLE 09-Dec-12 NT NEG NVD
MAU 27/2012 CATTLE 09-Dec-12 NT NEG NVD
PAKISTAN* PAK 9/2012 CATTLE 16-Jan-12 A POS A
PAK 10/2012 CATTLE 07-Feb-12 ASIA-1 POS ASIA-1
PAK 11/2012 CATTLE 09-Feb-12 ASIA-1 POS ASIA-1
PAK 12/2012 CATTLE 21-Feb-12 O POS O
PAK 13/2012 CATTLE 24-Feb-12 A POS A
PAK 14/2012 CATTLE 20-Mar-12 A POS A
PAK 15/2012 CATTLE 20-Mar-12 A POS A
PAK 16/2012 BUFFALO 06-Apr-12 A POS A
PAK 17/2012 CATTLE 07-Apr-12 O POS O
PAK 18/2012 BUFFALO 08-Apr-12 ASIA-1 POS ASIA-1
PAK 19/2012 BUFFALO 08-Apr-12 ASIA-1 POS ASIA-1
PAK 20/2012 CATTLE 11-Apr-12 O POS O
PAK 21/2012 CATTLE 12-Apr-12 ASIA-1 POS ASIA-1
PAK 22/2012 CATTLE 26-Apr-12 ASIA-1 POS ASIA-1
PAK 23/2012 CATTLE 27-Apr-12 O POS O
PAK 24/2012 CATTLE 27-Apr-12 ASIA-1 POS ASIA-1
PAK 25/2012 CATTLE 27-Apr-12 ASIA-1 POS ASIA-1
PAK 26/2012 CATTLE 27-Apr-12 ASIA-1 POS ASIA-1
PAK 27/2012 CATTLE 08-May-12 ASIA-1 POS ASIA-1
PAK 28/2012 CATTLE 09-May-12 NEG POS FMDV GD
WRLFMD Quarterly Report January-March 2013 2013
16
PAK 29/2012 BUFFALO 14-May-12 ASIA-1 POS ASIA-1
PAK 30/2012 CATTLE 14-May-12 ASIA-1 POS ASIA-1
PAK 31/2012 CATTLE 30-May-12 NEG NEG NVD
PAK 32/2012 CATTLE 20-Jun-12 A POS A
PAK 33/2012 CATTLE 20-Jun-12 A POS A
PAK 34/2012 BUFFALO 21-Jun-12 A POS A
PAK 35/2012 BUFFALO 21-Jun-12 ASIA-1 POS ASIA-1
PAK 36/2012 BUFFALO 30-Jun-12 ASIA-1 POS ASIA-1
PAK 37/2012 BUFFALO 30-Jun-12 ASIA-1 POS ASIA-1
PAK 38/2012 CATTLE 28-Sep-12 A POS A
PAK 39/2012 CATTLE 05-Nov-12 A POS A
PAK 40/2012 CATTLE 23-Nov-12 O POS O
PAK 41/2012 CATTLE 23-Nov-12 O POS O
UNITED ARAB
EMIRATES
UAE 1/2013 SCIMITAR HORNED ORYX
29-Jan-13 O POS O
UAE 2/2013 SCIMITAR
HORNED ORYX 29-Jan-13 O POS O
TOTAL : 129
*Pakistan samples pending from previous report
FMD(V) Foot-and-mouth disease (virus)
FMDV GD Genome detected
VI/ELISA FMDV serotype identified following virus isolation in cell culture and antigen ELISA
RT-PCR Reverse transcription polymerase chain reaction on epithelial suspension for FMD (or
SVD) viral genome
NVD No foot-and-mouth disease, swine vesicular disease or vesicular stomatitis virus
detected
NT Not tested
TABLE B: Summary of samples collected and received to The Pirbright Institute (January-March 2013)
Country No. of Virus isolation in cell culture/ELISA
RT-PCR for FMD
(or SVD)
samples FMD virus serotypes NVD
virus (where
appropriate)
O A C SAT 1 SAT 2 SAT 3 Asia 1 Positive Negative
BHUTAN 8 1 - - - - - - 7 8 -
IRAN 27 5 18 - - - - - 4 23 4
MAURITANIA 27 - - - - - - - 27 - 27
PAKISTAN 33 6 10 - - - - 15 2 32 1
TANZANIA 32 - 15 - 2 1 - - 14 21 5
UNITED ARAB
EMIRATES 2 2 - - - - - - - 2 -
TOTAL 129 14 43 - 2 1 - 15 54 86 37
VI/ELISA FMD (or SVD) virus serotype identified following virus isolation in cell culture and antigen
detection ELISA
FMD foot-and-mouth disease
SVD swine vesicular disease
NVD no FMD, SVD or vesicular stomatitis virus detected
NT not tested
RT-PCR reverse transcription polymerase chain reaction for FMD (or SVD) viral genome
WRLFMD Quarterly Report January-March 2013 2013
17
TABLE C: Antigenic characterisation of FMD field isolates by matching with vaccine strains by 2dmVNT
from 1st January to 31
st March 2012
Type O:
Vaccine Matching studies for serotype O FMDV by VNT-WRLFMD®
SAMPLE REF SEROTYPE O Manisa O Tur 5/09 O 4625
O Srl 2/12 O N M M
O Srl 3/09 O M M M
O Srl 9/10 O M M M
Type A:
Vaccine matching studies for serotype A FMDV by VNT-WRLFMD®
SAMPLE REF SEROTYPE A IND 17/82
A IRN 2005
A MAY 97
A TUR 06
A22 IRQ A ERI
98
PAK 13/2012 A N M N M M
PAK 32/2012 A M N N M N
PAK 39/2012 A N N M N N
TAN 40/2012 A M N M M N
VIT 15/2012 A N N N N
VIT 16/2012 A N N N N
TYPE SAT 2:
Vaccine Matching studies for serotype SAT2 FMDV by VNT-WRLFMD®
SAMPLE REF SEROTYPE SAT2 Eritrea SAT2 ZIM
TAN 36/2012 SAT2 N N
EGY 31/2012 SAT2 M M
Results Descriptor:
M : = Vaccine Match- r1 = > 0.3. Suggests that there is a close relationship between field isolate and vaccine
strain. A potent vaccine containing the vaccine strain is likely to confer protection.
N : = No Vaccine Match - r1 = < 0.3. Suggests that the field isolate is so different from the vaccine strain that
the vaccine is unlikely to protect
= Not tested against this vaccine
WRLFMD Quarterly Report January-March 2013 2013
18
Annex 2. Recent FMD Publications cited by PubMed
1. Gowane GR, Sharma AK, Sankar M, Narayanan K, Das B, Subramaniam S, Pattnaik B. Association of
BoLA DRB3 alleles with variability in immune response among the crossbred cattle vaccinated for foot-
and-mouth disease (FMD). Res Vet Sci. 2013 Mar 28. doi:pii: S0034-5288(13)00078-7.
10.1016/j.rvsc.2013.03.001. [Epub ahead of print] PubMed PMID: 23541924.
2. Wanczyk H, Barker T, Rood D, Zapata DI, Howell AR, Richardson SK, Zinckgraf J, Marusov GP, Lynes
MA, Silbart LK. Cloning and Characterization of a Hybridoma Secreting a 4-(Methylnitrosamino)-1-(3-
pyridyl)-1-butanone (NNK)-Specific Monoclonal Antibody and Recombinant F(ab). Toxins (Basel). 2013
Mar19;5(3):568-89. doi: 10.3390/toxins5030568. PubMed PMID: 23518474.
3. Larocco M, Krug PW, Kramer E, Ahmed Z, Pacheco JM, Duque H, Baxt B, Rodriguez LL. A Continuous
Bovine Kidney Cell Line Constitutively Expressing Bovine αVss6 Integrin Has Increased Susceptibility To
Foot and Mouth Disease Virus. J Clin Microbiol. 2013 Mar 20. [Epub ahead of print] PubMed PMID:
23515553.
4. Belsham GJ. Influence of the Leader protein coding region of foot-and-mouth disease virus on virus
replication. J Gen Virol. 2013 Mar 20. [Epub ahead of print] PubMed PMID: 23515027.
5. Zhou JH, You YN, Chen HT, Zhang J, Ma LN, Ding YZ, Pejsak Z, Liu YS. The effects of the synonymous
codon usage and tRNA abundance on protein folding of the 3C protease of foot-and-mouth disease virus.
Infect Genet Evol. 2013 Mar 14;16C:270-274. doi: 10.1016/j.meegid.2013.02.017. [Epub ahead of print]
PubMed PMID: 23499709.
6. Alejo DM, Moraes MP, Liao X, Dias CC, Tulman ER, Segundo FD, Rood D, Grubman MJ, Silbart LK. An
adenovirus vectored mucosal adjuvant augments protection of mice immunized intranasally with an
adenovirus-vectored foot-and-mouth disease virus subunit vaccine. Vaccine. 2013 Apr 26;31(18):2302-9.
doi:10.1016/j.vaccine.2013.02.060. Epub 2013 Mar 13. PubMed PMID: 23499593.
7. Chang H, Ma Y, Lin T, Cong G, Du J, Ma J. Foot-and-mouth disease virus carrier status in Bos grunniens
yaks. Virol J. 2013 Mar 11;10:81. doi: 10.1186/1743-422X-10-81. PubMed PMID: 23497369; PubMed
Central PMCID: PMC3602197.
8. Gülçe İz S, Döşkaya M, Borrego B, Rodriguez F, Gürüz Y, Gürhan ID. Co-expression of the Bcl-xL
antiapoptotic protein enhances the induction of Th1-like immune responses in mice immunized with DNA
vaccines encoding FMDV B and T cell epitopes. Vet Res Commun. 2013 Mar 13. [Epub ahead of print]
PubMed PMID:23483312.
9. Wang H, Liu X, Wu J, Wu G, Yu L, He C, Yang H, Xie W, Xia X, He H. Bovine fetal epithelium cells
expressing shRNA targeting viral VP1 gene resisted against foot-and-mouth disease virus. Virology. 2013
May 10;439(2):115-21. doi:10.1016/j.virol.2013.02.003. Epub 2013 Mar 6. PubMed PMID: 23481248.
10. Tekleghiorghis T, Moormann RJ, Weerdmeester K, Dekker A. Serological Evidence Indicates that Foot-
and-Mouth Disease Virus Serotype O, C and SAT1 are most Dominant in Eritrea. Transbound Emerg Dis.
2013 Mar 11. doi: 10.1111/tbed.12065.[Epub ahead of print] PubMed PMID: 23480728.
11. Diaz-San Segundo F, Dias CC, Moraes MP, Weiss M, Perez-Martin E, Owens G, Custer M, Kamrud K, de
Los Santos T, Grubman MJ. Venezuelan Equine Encephalitis Replicon Particles Can Induce Rapid
Protection against Foot-and-Mouth Disease Virus. J Virol. 2013 May;87(10):5447-60. doi:
10.1128/JVI.03462-12. Epub 2013 Mar 6. PubMed PMID: 23468490.
12. Rajasekhar R, Hosamani M, Basagoudanavar SH, Sreenivasa BP, Tamil Selvan RP, Saravanan P,
Venkataramanan R. Rescue of infective virus from a genome-length cDNA clone of the FMDV serotype O
(IND-R2/75) vaccine strain and its characterization. Res Vet Sci. 2013 Feb 25. doi:pii: S0034-
5288(13)00028-3.10.1016/j.rvsc.2013.01.026. [Epub ahead of print] PubMed PMID: 23465777.
13. Morelli MJ, Wright CF, Knowles NJ, Juleff N, Paton DJ, King DP, Haydon DT. Evolution of foot-and-
mouth disease virus intra-sample sequence diversity during serial transmission in bovine hosts. Vet Res.
WRLFMD Quarterly Report January-March 2013 2013
19
2013 Mar 1;44(1):12. doi:10.1186/1297-9716-44-12. PubMed PMID: 23452550; PubMed Central PMCID:
PMC3630017.
14. Yen HH, Scheerlinck JP. Biological activity of ovine IL-23 expressed using a foot-and-mouth disease virus
2A self-cleaving peptide. Cytokine. 2013 Mar;61(3):744-6. doi: 10.1016/j.cyto.2013.01.014. Epub 2013
Feb 16. PubMed PMID:23419450.
15. Chu JQ, Lee YJ, Park JN, Kim SM, Lee KN, Ko YJ, Lee HS, Cho IS, Kim B, Park JH. Construction of a
bovine enterovirus-based vector expressing a foot-and-mouth disease virus epitope. J Virol Methods. 2013
Apr;189(1):101-4. doi:10.1016/j.jviromet.2013.01.010. Epub 2013 Feb 4. PubMed PMID: 23391822.
16. Fu M, Zhang X, Lai X, Wu X, Feng F, Peng J, Zhong H, Zhang Y, Wang Y, Zhou Q, Wang S, Chen L, He
Z, Gao Y, Ma X, He R, Liu Q. Generation of Sequence Variants via Accelerated Molecular Evolution
Methods. Recent Pat DNA Gene Seq. 2013 Feb 1. [Epub ahead of print] PubMed PMID: 23388030.
17. Xu L, Hurtle W, Rowland JM, Casteran KA, Bucko SM, Grau FR, Valdazo-González B, Knowles NJ, King
DP, Beckham TR, McIntosh MT. Development of a universal RT-PCR for amplifying and sequencing the
leader and capsid-coding region of foot-and-mouth disease virus. J Virol Methods. 2013 Apr;189(1):70-6.
doi:10.1016/j.jviromet.2013.01.009. Epub 2013 Feb 1. PubMed PMID: 23380590.
18. Polacek C, Gullberg M, Li J, Belsham GJ. Low levels of foot-and-mouth disease virus 3Cpro expression are
required to achieve optimal capsid protein expression and processing in mammalian cells. J Gen Virol.
2013 Jan 30. [Epub ahead of print] PubMed PMID: 23364188.
19. Ehizibolo DO, Perez AM, Carrillo C, Pauszek S, Alkhamis M, Ajogi I, Umoh JU, Kazeem HM, Ehizibolo
PO, Fabian A, Berninger M, Moran K, Rodriguez LL, Metwally SA. Epidemiological Analysis, Serological
Prevalence and Genotypic Analysis of Foot-and-Mouth Disease in Nigeria 2008-2009. Transbound Emerg
Dis. 2013 Jan 24. doi: 10.1111/tbed.12054. [Epub ahead of print] PubMed PMID: 23347819.
20. Fukai K, Morioka K, Onozato H, Yoshida K, Sakamoto K. Comparative Evaluation of Three Commercial
ELISA Kits for Detection of Antibodies to a Nonstructural Protein of Foot-and-Mouth Disease Virus. J Vet
Med Sci. 2013 Jan 17. [Epub ahead of print] PubMed PMID: 23328606.
WRLFMD Quarterly Report January-March 2013 2013
20
High Priority O Manisa
O PanAsia-2
O BFS or Campos
A24 Cruzeiro
Asia 1 Shamir
A Iran-05
A22 Iraq
SAT 2 Saudi Arabia (or equivalent i.e. SAT 2 Eritrea)
(not in order of importance)
A15 Bangkok related strain
A87 Argentina related strain
C Noville
SAT 2 Kenya
SAT 1 Kenya
SAT 3 Zimbabwe
A Kenya (not in order of importance)
Medium Priority
Low Priority
A Eritrea
A Iran ’96
SAT 2 Zimbabwe
A Iran 87 or A Saudi Arabia 23/86 (or equivalent)
SAT 1 South Africa
A Malaysia 97 (or Thai equivalent such as A/NPT/TAI/86)
A Argentina 2001
O Taiwan 97 (pig-adapted strain or Philippine equivalent)
A Iran ’99
(not in order of importance)
Annex 3. RECOMMENDATIONS FROM WRLFMD® ON FMD VIRUS STRAINS TO BE INCLUDED IN FMDV ANTIGEN BANKS – March 2013