458 PATENT ABSTRACTS

A brevibacterium containing a recombinant DNA molecule constructed by operatively con- necting a gene coding for shikimate kinase isolated from Brevibacterium lactofermentum to a plasmid vector capable of replicating in the Brevibacterium is disclosed along with methods of preparing this bacterium and of using it in the production of aromatic amino acids.

5017482

P R O C E S S F O R P R O D U C I N G L - A R G I N I N E

Ryoichi Katsumata, Haruhiko Yokoi, Machida, Japan assigned to Kyowa Hakko Kogyo Co Ltd

5017487

V A C C I N I A D N A

Hendrik G Stunnenberg, Riccardo Wittek, Bammental, Federal Republic Of Germany as- signed to Hoffmann-La Roche lnc

New transcriptional regulatory sequences useful in the expression of pro- or eukaryotic proteins in eukaryotic organisms are provided com- prising the 5" flanking region of the vaccinia virus late II kDa gene. Also described are recombinant vectors and recombinant infectious poxviruses containing such transcriptional regulatory sequences operatively linked to fore- ign genes, and live vaccines based upon these recombinant infectious poxviruses.

L-arginine is produced by constructing a recom- binant DNA composed of a vector DNA and a DNA fragment derived from chromosomal D N A of a microorganism belonging to the genus Corynebacterium or Brevibacterium and bearing genetic information relating to the syn- thesis of L-arginine-biosynthetic enzyme, in- troducing the recombinant DNA in a microorganism belonging to the genus Corynebacterium or Brevibacterium, culturing the microorganism in a medium, and recovering L-arginine accumulated in the culture broth.

5017486

C L O N I N G O F D N A E N C O D I N G A N T I B A C T E R I A L P O L Y P E P T I D E

P R E C U R S O R

5017488

H I G H L Y E F F I C I E N T D U A L T 7 / T 3 P R O M O T E R V E C T O R P J K F I 6

A N D D U A L S P 6 / T 3 P R O M O T E R V E C T O R P J F K I 5

William T McAIlister, John F Klement assigned to University of Medicine and Dentistry of New Jersey

A dual promoter cassette which has at one end a promoter for T3 RNA polymerase which con- tains a downstream sequence identical to a naturally occurring T3 promoter sequence and on the other end, a promoter for a phage DNA polymerase other than the T3 RNA polymerase. The recombinant DNA plasmid which includes the promoter. The plasmid is capable of highly efficient transcription of RNA with low con- centrations of ribonucleoside triphosphates.

Kiich Sawai, Shunji Natori, Haruo Takahashi. Kenichi Tanaka, Takahiko Mitani. Masayasu Kurono, Aichi, Japan assigned to Sanwa Kagaku Kenkyusho Co Ltd

A cloned single-strand DNA comprising nucleotide sequence which encodes an anti- bacterial polypeptide precursor, a cloned double-strand D N A consisting of the single- strand DNA and its complementary single- strand DNA, a DNA fragment of the single- or double-strand DNA, a process for the prepara- tion thereof, and a plasmid, in which the double- strand DNA or its fragment is inserted are disclosed.

5017489

C Y T O T O X I C T L Y M P H O C T E S E R I N E E S T E R A S E A N D

M E T H O D F O R S T I M U L A T I O N A N D I N H I B I T I O N

Mark S Pasternack, Herman S Eisen assigned to Massachusetts Institute of Technology

Antibodies, nucleic acid .sequences. and methods for inhibition of lysis for a novel serine esterase produced by both murinc and human cytotoxic