biotechnology review power point
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Biotechnology ReviewQUIZ FEBRUARY 12TH
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Ashley donated a regular body cell and Jesse donated an egg cell. Jesse’s egg cell had its nucleus removed. Ashley’s cell nucleus was transferred from the body cell into the egg cell. Cell division occurred and the new embryo was implanted into Madison, the surrogate mother. Madison eventually gave birth to the baby. Who does the baby resemble?
Ashley
Who committed the crime?
Click icon to add picture
Suspect #2
Goats that make spider silk is an example of…a. Genetic Engineeringb. Stem Cell Therapyc. DNA fingerprintingd. PCR
A. Genetic Engineering
What might we be able to treat Parkinson’s Disease with?A. Genetic EngineeringB. Stem CellsC. CloningD. Recombinant DNA
B. Stem Cells
What is a genome?
All of the genes within an organism
What is the Human Genome Project?
Mapping of all of the genes within humans
Why does DNA move through the gel during gel electrophoresis?
Negative charge, moves towards positive electrode
Who is this?
Dolly
Making an identical copy of an organism is known as what?
Cloning
What is so great about a stem cell?
Function has not yet been determined, can differentiate into different cell types
What do we use to cut DNA?
Restriction Enzymes
What organisms are restriction enzymes naturally found in?
Bacteria
What purpose do restriction enzymes serve in bacteria?
Protection from viruses
If a restriction enzyme cuts at the sequence CC/GG what kind of ends would we end up with on the DNA fragments?
Blunt Ends
EcoRI recognizes the following sequence: G A A T T C C T T A A G
What types of ends would this cut result in?
Sticky
The longest fragment is in which lane?
Lane D
The shortest fragment is in which lane?
Lane A
What is the purpose of a DNA size standard?
Gives known fragment lengths of DNA
The left column is the DNA Standard of 100, 50, and 10. What would the band lengths be in the second column?
About 75 and 50
What is a plasmid?
Circular DNA in bacteria
EcoR1 recognizes the sequence GAATTC. How many fragments would this piece of DNA be cut into?
2 cuts, so 3 fragments
What are the basic steps in cloning?
Place nucleus of somatic cell into egg cell that has it’s nucleus removed, place into surrogate
What is the difference between an egg cell and a somatic cell?
Somatic cell has full set of chromosomes, egg cell has a half set of chromosomes
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