Biotechnology ReviewQUIZ FEBRUARY 12TH

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Ashley donated a regular body cell and Jesse donated an egg cell. Jesse’s egg cell had its nucleus removed. Ashley’s cell nucleus was transferred from the body cell into the egg cell. Cell division occurred and the new embryo was implanted into Madison, the surrogate mother. Madison eventually gave birth to the baby. Who does the baby resemble?

Ashley

Who committed the crime?

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Suspect #2

Goats that make spider silk is an example of…a. Genetic Engineeringb. Stem Cell Therapyc. DNA fingerprintingd. PCR

A. Genetic Engineering

What might we be able to treat Parkinson’s Disease with?A. Genetic EngineeringB. Stem CellsC. CloningD. Recombinant DNA

B. Stem Cells

What is a genome?

All of the genes within an organism

What is the Human Genome Project?

Mapping of all of the genes within humans

Why does DNA move through the gel during gel electrophoresis?

Negative charge, moves towards positive electrode

Who is this?

Dolly

Making an identical copy of an organism is known as what?

Cloning

What is so great about a stem cell?

Function has not yet been determined, can differentiate into different cell types

What do we use to cut DNA?

Restriction Enzymes

What organisms are restriction enzymes naturally found in?

Bacteria

What purpose do restriction enzymes serve in bacteria?

Protection from viruses

If a restriction enzyme cuts at the sequence CC/GG what kind of ends would we end up with on the DNA fragments?

Blunt Ends

EcoRI recognizes the following sequence: G A A T T C C T T A A G

What types of ends would this cut result in?

Sticky

The longest fragment is in which lane?

Lane D

The shortest fragment is in which lane?

Lane A

What is the purpose of a DNA size standard?

Gives known fragment lengths of DNA

The left column is the DNA Standard of 100, 50, and 10. What would the band lengths be in the second column?

About 75 and 50

What is a plasmid?

Circular DNA in bacteria

EcoR1 recognizes the sequence GAATTC. How many fragments would this piece of DNA be cut into?

2 cuts, so 3 fragments

What are the basic steps in cloning?

Place nucleus of somatic cell into egg cell that has it’s nucleus removed, place into surrogate

What is the difference between an egg cell and a somatic cell?

Somatic cell has full set of chromosomes, egg cell has a half set of chromosomes