irmi units biomat

BIOMATERIALI AD USO BIOMEDICALE BASATI SU

POLISACCARIDI NATURALI (ortopedia, odontoiatria e della chirurgia interna)

Biomat group

biomat@units.it

1-­‐deoxylac+t-­‐1-­‐yl  chitosan:  “CHITLAC”    

�  CHITLAC  IS  A  POLYSACCHARIDE  WHICH  DERIVES  FROM  CHITOSAN  

OOOH

O

NH2

OO

OHNH

OH

OH

OOH

O

NHOOH

NH2

OH

OH

O

OH

O

OH

OH

OH

OH

O

OH

OH

OH

HH

•  SOLUBLE AT PHYSIOLOGICAL pH

•  MISCIBLE WITH ANIONIC

POLYSACCHARIDES

•  BIOCOMPATIBLE

•  BIOACTIVE

lactose

Modificazione chimica per modulare l’attività biologica dei polimeri- materiali BIOATTIVI

es. Chitlac-RGD    

Chemical modification of polymers:

conjugation of RGD peptides with

Chitlac

Alginate Polyanion

Gel forming propeties

Chitlac Polycation

Bioactive polymer

Soluble mixtures

ECM-mimic

Bioactivity

Mechanical support

Biopolymer-based scaffold for tissue engineering and cell encapsulation

O

O O

O O

OH

OH O

OH

O

OH O

¯OOC

¯OOC ¯OOC

¯OOC

OH

OH

OH

OH

COO¯ COO¯

COO¯

HO HO

HO HO

HO HO

O

O O

O O

O O

O

HO HO

COO¯

Material preparation and manufacturing and characterization of the final construct

Chemical-physical characterizations • Reology

• NMR • Spectroscopy

Mechanical and structural/ morphological characterizations • SEM • TEM

• Confocal microscopy • Mechanical tests (tensile strength young modulus..)

Biological in vitro testing • Cell viability and proliferation

• Synthesis of ECM components • Differentiation

• Inflammatory response

UNITS experimental activity

Project  1tle  

Development of a resorbable sealing patch for the prevention of anastomotic leakage after

colorectal cancer surgical treatment

10  

Medical  need  

10  

Colorectal cancer:

•  Second most common form of cancer in Europe (2.9 million new cases/year)

•  Surgical treatment: resection of the affected bowel and suture of the extremities to restore the normal transit (Anastomosis)

•  Most feared complication: ANASTOMOTIC LEAKAGE (up to 23% of cases à mortality 39%)

Tumor  

Anastomosis  

Surgical  removal  

COMPLICATION  

ANASTOMOTIC    LEAKAGE  

11  

A  biopolymeric  bioactive  patch  wrapped  around  the  sutured  part  of  the  intestine  capable  of  inducing  a  faster  tissue  regeneration,  thus  

promoting  a                                                      safe  ananstomosis  in  the  critical  period  of  tissue  healing  

Proposed  solu1on  

AnastomoSEAL project

Biopolymeric membranes for the treatment of colorectal anastomosis

The AnastomoSEAL Consortium

University of Trieste (Italy) Main role: Coordination, Material development, Clinical Guidance

Impuls (Poland) Main role: Sterilization and

Processing

University of Maastricht (The Netherlands)

Main role: Biological studies (in vivo)

Rescoll (France) Main role: Technological

implementation

FMC-Novamatrix (Norway) Main role: Biopolymer

manufacturing, Guidance towards product

Sigea (Italy) Main role: Development of

innovative bioactive polymers

Biomat lab, DSV

PROMETEO BCTM: OR 1 & 2 CIRI – SDV: OR 2

BIOMAT-UNITS Potential Collaborations