c astlehead h igh s chool enzymes we couldn’t live with out them! intermediate 2
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Enzymes
We couldn’t live with out them!
Intermediate 2
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What is an enzyme?
• Known as a BIOLOGICAL CATALYST
• Catalyst is something that speeds up a chemical reaction
• Biological catalyst speeds up reactions in living cells
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Activation Energy
• Do this by lowering the ACTIVATION ENERGY - the energy required for a reaction to begin
Progress of reaction
En
erg
y
Uncatalysed reaction
Catalysed reaction
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Properties of Enzymes
• They are made of PROTEIN
• They are not changed by the reactions they speed up
• They are SPECIFIC- means that only one particular enzyme will work with one particular substrate
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Other terms you need to know
• Substrate – the substance that the enzyme works on e.g. Amylase the substrate is starch
• Product – the substance that is made by the reaction e.g. Breakdown of starch by Amylase the product is Maltose
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CATALASE
• Found in animal and plant cells
• Needed to speed up the breakdown of HYDROGEN PEROXIDE
• Breaks it down to OXYGEN and WATER
• The word equation for this looks like this
HYDROGEN PEROXIDE
CATALASE
OXYGEN AND WATER
HPCOW
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Amylase
• Found in saliva and in the pancreas
• Break down enzyme
• Breaks STARCH down to MALTOSE
• Word equation looks like this
STARCH MALTOSE
AMYLASESAM
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Potato Phosphorylase
• Synthesis enzyme (builds up)
• Builds Glucose-1-Phosphate molecules into Starch
• The formation of starch is tested using iodine solution
Phosphorylase
G1PPS
Glucose-1-Phosphate Starch
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More about Specific
• This means amylase will only breakdown starch
• Catalase will only breakdown hydrogen peroxide
• Amylase will NOT breakdown hydrogen peroxide
• Catalase will NOT breakdown starch
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Lock and Key
• All enzymes have a special shaped area that fits onto their substrate
• This area is called the ACTIVE SITE
• This Active site will fit onto the substrate while the reaction takes place
• Because it fits like a lock and key we call this the lock and key mechanism
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Model of Lock and Key
productssubstrate
enzyme enzyme – unchangedactive site
enzyme substrate complex
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Effect of Temperature
• Speed of reaction increases until an Optimum temperature is reached
• Optimum temperature is the temperature at which the enzyme works best
• After this point the rate of reaction decreases until there is no reaction
• At this point enzyme is said to be DENATURED – active site destroyed
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Effect of pH
• Most enzymes have an optimum pH of 7
• Some enzymes have a different optimum pH for example pepsin has an optimum pH of 2
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More to do
• Click on links to go to website and do Biology keystage 3 lessons 14,15,16,39 and 40
http://lgfl.skoool.co.uk/keystage3
• Also go to BBC bitesize standard grade biology topic investigating cells Enzymes and Aerobic Respiration
http://www.bbc.co.uk/scotland/education/bitesize/