篠崎連携研究グループ（機能開発研究チーム） -...

― 189 ―

　植物の系では外部環境への応答や適応、光合成、植物

ホルモンによる成長制御、高効率での分化全能性など動

物の系とは明らかに異なった植物独自の個体レベル、細

胞レベル、分子レベルでの制御機構が存在している。

　当協力研究グループではモデル植物のシロイヌナズナ

を用いて遺伝子破壊型変異体や過剰発現型変異体（ミ

ニ FOXシステム）のリソース開発、シロイヌナズナ・

ポプラ・ダイズ・コムギ・その他植物種の完全長 cDNA

Kazuo SHINOZAKI, Ph.D.グループヘッド　篠崎　一雄（理博）

Bioresource Research Collaborative Groupバイオリソース連携研究グループ

職員とメンバー構成グループヘッド篠崎　一雄（平成 17年 4月～）

研究員梅澤　泰史（平成 17年 4月～）

藤田　美紀（平成 18年 4月～）

黒森　崇（平成 17年 4月～）

明賀　史純（平成 17年 4月～）

特別研究員浦野　薫（平成 17年 4月～）

高橋　史憲（平成 19年 5月～）

（平成 16年 4月～平成 19年 3月　ジュニアリサーチアソシエイト）

基礎科学特別研究員西山　りゑ（平成 18年 4月～）

日本学術振興会特別研究員 Behnam Babak（平成 19年 10月～）

特任職員水門　佐保（平成 20年 4月～）

（平成 18年 4月～平成 20年 3月　PSCテクニカルスタッフ）

ジュニアリサーチアソシエイト溝口　昌秀（平成 19年 4月～）

研修生中村　正輝（平成 17年 4月～平成 19年 3月）

業務委託正代　初代（平成 12年 1月～）

小林　裕子（平成 15年 4月～）

小澤　久美子（平成 15年 4月～）

植物分子生物学研究室

先任研究員伊藤　卓也（平成 12年４月～平成 19年 3月 [ 現・共同研究者 ]）

篠崎連携研究グループ（機能開発研究チーム）

Shinozaki Research Collaborative Group

リソース開発を通じてバイオリソースセンターのリソー

ス業務に貢献している。さらに、表現型、遺伝子発現、

代謝産物、タンパク質などをそれぞれ網羅的に解析する

「オミクス解析」および、個々の遺伝子の詳細な解析に

よって得られた情報を統合することで、これらリソース

に付加価値をつける。また、環境ストレス応答機構の研

究を通じて人類が直面している環境・食料問題に取組ん

でいる。

篠崎連携研究グループ（機能開発研究チーム）Shinozaki Research Collaborative Group

RIKEN BRC Annual Report 2006～ 2007

― 190 ―

年次計画と成果

1. トランスポゾン（Ds）挿入シロイヌナズナ変異体コレクションの表現型解析（フェノーム解析）

　ポストゲノム解析として我々は、シロイヌナズナ遺伝

子へのトランスポゾン Ds挿入変異体作製に引き続き、

Dsの同型接合体（ホモライン）を用いてフェノーム解

析（全遺伝子破壊変異体を用いた網羅的表現型解析）を

行っている。形態変化を指標にした表現型解析について、

我々が作製した約 18,000 Ds挿入変異体コレクションの

うち約 12,000ラインの解析は前年度に報告した。今年

度からは残り 6,000ラインの解析を開始し、現在継続中

である。

　フェノーム解析の過程で、雄性不稔を示す male

sterility1 (ms1)変異体（図 1）を単離し、今年度はその

詳細な機能解析を行った。雄性不稔の原因遺伝子 MS1

は転写活性化因子をコードし，花粉成熟に必要な下流遺

伝子を制御していることが明らかとなった。

梅澤、西山、伊藤、Behnam Babak、水門、小林、松尾、衞藤、浦野

高橋、藤田、溝口、篠崎グループヘッド、正代、小澤

図 1　シロイヌナズナの成熟途中の花粉と葯の透過電子顕微鏡写真

MI: 小胞子。TA: タペート層。Exine: エキシン。

（A, C）野生型（B, D) ms1 突然変異体

（Aと Bを比較）ms1 突然変異体では、小胞子（矢印）とタペート層（矢じり）

が空胞化して、最終的には成熟花粉が全くできない。

（Cと Dを比較）ms1 変異体では、野生型で見られる特徴的なエキシン構

造が見られない。



― 191 ―

ダイズRNAプールの準備

完全長cDNAライブラリーの作成

完全長cDNAのクローン化

完全長cDNAクローンの端読み

独立クローンの抽出

全長配列の決定分子マーカー etc. カスタムオリゴアレイ

ダイズRNAプールの準備

完全長cDNAライブラリーの作成

完全長cDNAのクローン化

完全長cDNAクローンの端読み

独立クローンの抽出

全長配列の決定分子マーカー etc. カスタムオリゴアレイ

図 2　ダイズ cDNAプロジェクトの概要

ムシグナル伝達とのクロストークについて研究を行っ

た。また、ストレス耐性獲得のシステムを明らかにする

ために、ストレス応答性カルシウム結合タンパク質の機

能解析、ストレス応答性アブシジン酸（ABA）合成酵

素の発現制御解析、ユビキチン化を介したストレス応答

性転写因子の分解系について研究を行った。

5. ストレス耐性植物の実用化に向けた形質転換作物の作製と圃場試験

　本研究グループではこれまでに、環境ストレス耐性に

関与する遺伝子を多く単離してきた。本研究では、これ

ら耐性遺伝子を利用したストレス耐性作物の実用化を目

指し、主要作物の一つであるコムギに注目して研究を

行っている。本年度は、コムギに乾燥耐性を付与させる

ための最適な遺伝子をシロイヌナズナより選抜し、導入

用ベクターを作製した。また乾燥ストレス付与に関わる

38遺伝子の完全長 cDNAを、コムギ相同遺伝子として

収集した。更にコムギのマイクロアレイ解析情報などを

用いて、恒常的発現型プロモーターの選抜を行った。今

後はこれらを組み合わせてコムギ品種等に導入し、得ら

れた形質転換体について圃場条件における乾燥耐性や収

量等を評価していく（図 3）。

6. トランスクリプトミクスとメタボロミクスの統合による ABA依存的な乾燥ストレス応答性代謝ネットワークの解明

　乾燥ストレス下の植物において、ABAは細胞内シグ

ナル伝達や多くの遺伝子の発現誘導に重要な働きを行

う植物ホルモンである。本研究では、シロイヌナズナ

ABA合成関連遺伝子 NCED3の破壊株を実験材料とし、

トランスクリプトミクスとメタボロミクス解析を組み合

わせることで、乾燥ストレス下で合成された ABAが制

御する代謝ネットワークの包括的な解明を行った。

2. ダイズ完全長 cDNAプロジェクト

　理研 PSCでは、ポストゲノム研究にとって有用なリ

ソースとなる完全長 cDNAの整備を、複数の有用作物

について進めている。本研究グループでは、代表的なマ

メ科作物であるダイズの完全長 cDNAプロジェクトを

担当し、約 40,000クローンについて塩基配列の収集お

よびデータ解析を終了した。現在、全てのデータの公開

に向けたシステムを構築中である（図 2）。

3. 過剰発現型トランスジェニック植物を利用したミニ FOXシステムの開発

　本研究グループでは、次世代型アクチベーションタ

ギング法である FOX Hunting System（full-length cDNA

over-expressor gene hunting system）を利用して、環境ス

トレス応答性を示すシロイヌナズナ転写因子の機能解析

を行った。41個の環境ストレス誘導性転写因子の完全

長 cDNAを高発現する植物ライブラリーを作製し、塩

ストレス耐性に関与する bZIP60遺伝子の単離に成功し

た。

4. 環境ストレス応答に関わる遺伝子の機能解析　植物は乾燥、塩、低温ストレスなどの様々な環境スト

レスに応答して、植物ホルモンを介したシグナル伝達と

遺伝子発現を行い環境に適応している。本研究グループ

では植物の生産力向上のため、環境ストレス応答に関連

した遺伝子探索と機能解析を中心に研究を行っている。

本年度は環境ストレス応答におけるシグナル伝達経路を

明らかにするために、環境ストレスと植物ホルモンで活

性化するプロテインキナーゼの機能解析および、ジャス

モン酸シグナル伝達に関わるMAPキナーゼとカルシウ



― 192 ―

図 3　環境ストレス耐性コムギの作出

乾燥ストレス耐性植物の分子育種のために、ストレス誘導性プロモーターや機能遺伝子を選抜する。

得られた知見をもとに、圃場で有効なストレス耐性コムギを作出する。

Ito Y., Katsura K., Maruyama K., Taji T., Kobayashi

M., Seki M., Shinozaki K. Yamaguchi-Shinozaki

K.:“Functional analysis of rice DREB1/CBF-type

transcription factors involved in cold-responsive gene

expression in transgenic rice”, Plant Cell Physiol., 47,

141-153 (2006).*

Yoshida T., Nishimura N., Kitahata N., Kuromori T.,

Ito T., Asami T., Shinozaki K. Hirayama T.: “ABA

Hypersensitive Germination3 encodes a protein

phosphatase 2C (AtPP2CA) that strongly regulates

ABA signaling during germination among Arabidopsis

PP2Cs”, Plant Physiol., 140, 115-126 (2006).*

Umezawa T., Okamoto M., Kushiro T., Nambara E.,

Oono Y., Seki M., Kobayashi M., Koshiba T., Kamiya Y.

Shinozaki K.: “CYP707A3, a major ABA 8’-hydroxylase

involved in dehydration rehydration response in

Arabidopsis thaliana”, Plant J., 46, 171-182 (2006).*

Furihata T., Maruyama K., Fujita Y., Umezawa T.,

Yoshida R., Shinozaki K. Yamaguchi-Shinozaki K.:

研究発表（誌上発表）

1.

2.

3.

4.

【原著論文】 * は査読制度がある論文誌“ABA-dependent multisite phosphorylation regulates the

activity of a transcription activation AREB1”, Proc. Natl.

Acad. Sci. USA., 103, 1988-1993 (2006).*

Yoshida R., Umezawa T., Mizoguchi T., Takahashi S.,

Takahashi F. Shinozaki K.: “The regulatory domain

of SRK2E/OST1/SnRK2.6 interacts with ABI1 and

integrates ABA and osmotic stress signals controlling

stomatal closure in Arabidopsis”, J. Bio. Chem., 281,

5310-5318 (2006).*

Yamasaki K., Kigawa T., Inoue M., Yamasaki T., Yabuki

T., Aoki M., Seki E., Matsuda T., Tomo Y., Terada

T., Shirouzu M., Tanaka A., Seki M., Shinozaki K.

Yokoyama S.: “An Arabidopsis SBP-domain fragment

with a disrupted C-terminal zinc-binding site retains its

tertiary structure”, FEBS Lett., 580, 2109-2116 (2006).*

Hasegawa Y., Seki M., Mochizuki Y., Heida N., Hirosawa

K., Okamoto N., Sakurai T., Satou M., Akiyama K.,

Iida K., Lee K., Kanaya S., Demura T., Shinozaki K.,

Konagaya A. Toyoda T.: “A flexible representation of

5.

6.

7.



― 193 ―

omic knowledge for thorough analysis of microarray

data”, Plant methods, 2,5 (2006).*

Sakuma Y., Maruyama K., Osakabe Y., Qin F., Seki M.,

Shinozaki K. Yamaguchi-Shinozaki K.:“Functional

analysis of an Arabidopsis transcription factor, DREB2A,

involved in drought-responsive gene expression”, Plant

Cell, 18,1292-1309 (2006).*

Noutoshi Y., Kuromori T., Wada T., Hirayama T., Kamiya

A., Imura Y., Yasuda M., Nakashita H., Shirasu K.

Shinzoaki K.:“Loss of NECROTIC SPOTTED LESIONS

1 associates with cell death and defense responses in

Arabidopsis thaliana”, Plant Mol. Biol., 62, 29-42

(2006).*

Kuromori T., Wada T., Kamiya A., Yuguchi M., Yokouchi

T., Imura Y., Takabe H., Sakurai T., Akiyama K.,

Hirayama T., Okada K. Shinzoaki K.:“A trial of phenome

analysis using 4000 Ds-insertional mutants in gene-

coding regions of Arabidopsis”, Plant J., 47, 640-651

(2006).*

Kitahata N., Han S-Y., Noji N., Saito T., Kobayashi

M., Nakano T., Kuchitsu K., Shinozaki K., Yoshida

S., Matsumoto S., Tsujimoto M. Asami T.:“A 9-cis-

epoxycarotenoid dioxygenase inhibitor for use in

the elucidation of abscisic acid action mechanisms”,

Bioorganic & Medicinal Chem., 15, 5555-5561 (2006).*

Nishiuchi T., Masuda D., Nakashita H., Ichimura K.,

Shinozaki K., Yoshida S., Kimura M., Yamaguchi I.

Yamaguchi K.:“Fusarium phytotoxin trichothecenes

have an elicitor-like activity in Arabidopsis thaliana, but

the activity differed signifi cantly among their molecular

species”, Mol Plant Microve Interact., 19, 512-520

(2006).*

Machida M., Takechi K., Sato H., Chung S.J., Kuroiwa

H., Takio S., Seki M., Shinozaki K., Fujita T., Hasebe

M. Takano H.:“Gene for the peptidoglycan synthesis

pathway are essential for chloroplast division in moss”,

Proc. Natl. Acad. Sci. USA., 103, 6753-6758 (2006).*

8.

9.

10.

11.

12.

13.

14.

15.

16.

17.

18.

19.

20.

21.

Gross J., Cho W-K., Lezhneva L., Falk J., Krupinska

K., Shinzoaki K., Seki M., Herrmann R.G. Meurer J.:

“A plant locus essential for phylloquinone (Vitamin K1)

Biosynthesis orininated from a fusion of foure eubacterial

genes”, J. Biol. Chem., 281, 17189-17196 (2006).*

Oono Y., Seki M., Satou M., Iida K., Akiyama K.,

Sakurai T., Fujita M., Yamaguchi-Shinozaki K. Shinozaki

K.:“Monitoring expression profiles of Arabidopsis

genes during cold acclimation and deacclimation using

DNA microarrays”, Funct. Integr. Genomics, 6, 212-234

(2006).*

Myouga F., Motohashi R., Kuromori T., Nagata

N. Shinozaki K.:“An Arabidopsis chloroplast-

targeted Hsp101 homolog, APG6, has an essential

role in choloroplast development as well as heat stress

response”, Plant J., 48, 249-260 (2006).*

Sakuma Y., Maruyama K., Qin F., Osakabe Y., Shinozaki

K. Yamaguchi-Shinozaki K.: “Dual function of an

Arabidopsis transcription factor DREB2A in water-stress-

responsive and heat-stress-responsive gene expression”,

Proc. Natl. Acad. Sci. USA., 103. 18822-18827 (2006).*

Ichimura K., Casais C., Peck SC., Shinozaki K. Shirasu

K.:“MEKK1 is required for MPK4 activation and

regulates tissue specifi c and temperature dependent cell

death in Arabidopsis”, J. Bio. Chem., 281, 36969-36976

(2006).*

Ichikawa T., Nakazawa M., Kawashima M., Iizumi H.,

Kuroda H., Kondou Y., Tsuhara Y., Suzuki K., Ishikawa

A., Seki M., Fujita M., Motohashi R., Nagata N., Takagi

T., Shinozaki K. Matsui M.:“The FOX hunting system:

an alternative gain-of-function gene hunting technique”,

Plant J., 45, 974-986 (2006).*

Matsuda T., Kigawa T., Koshiba S., Inoue M., Aoki M.,

Yamasaki K., Seki M., Shinozaki K. Yokoyama S.: “Cell-

free synthesis of zinc-binding proteins”, J. Struct Funct

Genomics, 7, 93-100 (2006).*

Tran P. L-S., Nakashima K., Sakuma Y., Fujita Y.,



― 194 ―

Osakabe Y., Qin F., Simpson SD., Maruyama K., Fujita Y.,

Shinozaki K. Yamaguchi-Shinozaki K.: “Co-expression

of the stress-inducible zinc fi nger homeodomain ZFHD1

and NAC transcription factors enhances exporession

of the ERD1 gene in Arabidopsis”, Plant J., 49, 46-63

(2007).*

Qin F., Kakimoto M., Sakuma Y., Maruyama K., Osakabe

Y., Tran L-S., Shinzoaki K. Yamaguch-Shinozaki K.:

“Regulation and functional analysis of ZmDREB2A in

response to drought and heat stresses in Zea mays L.”,

Plant J.,50, 54-69 (2007).*

Mitsuda N.,Iwase A., Yamamoto H., Yoshida M., Seki

M., Shinozaki K. Ohme-Takagi M.: “NAC transcription

factors, NST1 and NST3, are key regulators of the

formation of secondary walls in woody tissues of

Arabidopsis”, Plant Cell, 19, 270-280 (2007).*

Takahashi F., Yoshida R., Ichimura K., Mizoguchi T., Seo

S., Yonezawa M., Maruyama K., Yamaguchi-Shinozaki

K. Shinozaki K.: “Dissecting the jasmonate signaling

pathway by a novel MAP kinase cascade, MKK3-MPK6,

in Arabidopsis”, Plant Cell, 19, 805-818 (2007).*

Itoh T., Tanaka, T., Barrero R.A., Yamasaki C., Fujii

Y., Hilton P.B., Antonio B.A., Aono H., Apweiler R.,

Bruskiewich R., Bureau T., Burr F., Fuks G., Habara T.,

Haberer G., Han B., Harada E., Hiraki A.T., Hirochika

H., Hoen D., Hokari H., Hosokawa S., Hsing Y.I., Ikawa

H., Ikeo K., Imanishi T., Ito Y., Jaiswal P., Kanno M.,

Kawahara Y., Kawamura T., Kawashima H., Khurana

J.P., Kikuchi S., , Komatsu S., Koyanagi K.O., Kubooka

H., Lieberherr D., Lin Y.C., Lonsdale D., Matsumoto

T., Matsuya A., McCombie W.R., Messing J., Miyao A.,

Mulder N., Nagamura Y., Nam J., Namiki N., Numa H.,

Nurimoto S., O'Donovan C., Ohyanagi H., Okido T., de

Oliveira A.C., Oota S., Osato N., Palmer L.E., Quetier

F., Raghuvanshi S.,Saichi N.,Sakai H., Sakai Y., Sakata

K., Sakurai T., Sato F., Sato Y., Schoof H., Seki M.,

Shibata M., Shimizu Y., Shinozaki K., Shinso Y., Singh

N.K., Smith-White B., Takeda J., Tanino M., Tatusova

T., Thongjuea S., Todokoro F., Tsugane M., Tyagi A.K.,

Vanavichit A., Wang A., Wing R.A., Yamaguchi K.,

22.

23.

24.

25.

26.

27.

28.

29.

30.

31.

Yamamoto M., Yamamoto N., Yu Y., Zhang H., Zhao

Q., Higo K., Burr B., Gojobori T. Sasaki T.: “Curated

Genome Annotation of Oryza sativa ssp. Japonica

and Comparative Genome Analysis with Arabidopsis

thaliana”. Genome Res. 17, 175-183 (2007).*

Nakagawa Y., Katagiri T., Shinozaki K., Qi Z., Tatsumi

H., Furuichi T., Kishigami A., Sokabe M., Kojima

I., Sato S., Kato T., Tabata S., Iida K., Terashima A.,

Nakano M., Ikeda M., Yamanaka T. Iida K.: “Arabidopsis

plasma membrane protein crucial for Ca2+ influx and

touch sensing in roots”, Proc. Natl. Acad. Sci. USA., 104,

3639-3644 (2007).*

Nakashima K., Tran P. L-S., Nguyen D.V., Fujita M.,

Maruyama K., Todaka D., Ito Y., Hayashi N., Shinozaki

K. Yamaguchi-Shinozaki K.: “Functional analysis of

a NAC-type transcription factor OsNAC6 involved in

abiotic and biotic stress-responsive gene expression in

rice”, Plant J., 51, 617-630 (2007).*

Mizuno S., Osakabe Y., Maruyama K., Ito T., Osakabe

K., Sato T., Shinozaki K. Yamaguchi-Shinozaki K.:

“Receptor-like protein kinase 2 (RPK 2) is a novel factor

controlling anther development in Arabidopsis thaliana”,

Plant J., 50, 751-766 (2007).*

Iuchi S., Koyama H., Iuchi A., Kobayashi Y., Kitabayashi

S., Kobayashi Y., Ikka T., Hirayama T., Shinozaki K.

Kobayashi M.: “Zinc finger protein STOP1 is critical

for proton tolerance in Arabidopsis and coregulates a

key gene in aluminum tolerance”, Proc. Natl. Acad. Sci.

USA., 104, 9900-9905 (2007).*

Okuda K., Myouga F., Motohashi R., Shinozaki

K. Shikanai T.: “Conserved domain structure of

pentatricopeptide repeat proteins involved in choroplast

RNA editing”, Proc. Natl. Acad. Sci. USA., 104, 8178-

8183 (2007).*

Tian C., Chikayama E., Tsuboi Y., Kuromori T.,

Shinozaki K., Kikuchi J. Hirayama T.: “Top-down

phenomics of Arabidopsis thaliana-METABOLIC

PROFILING BY ONE- AND TWO-DIMENSIONAL



― 195 ―

NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

AND TRANSCRIPTOME ANALYSIS OF ALBINO

MUTANTS”, J. Bio Chem., 282, 18532-18541 (2007).*

Nishimura N., Yoshida T., Kitahata N., Asami T.,

Shinozaki K. Hirayama T.: “ABA-Hypersensitive

Germination1 encodes a protein phosphatase 2C,

an essential component of abscisic acid signaling in

Arabidopsis seed”, Plant J., 50, 935-949 (2007).*

Motohashi R., Yamazaki T., Myouga F., Ito T., Ito

K., Satou M., Kobayashi M., Nagata N., Yoshida S.,

Nagashima A., Tanaka K., Takahashi S. Shinozaki K.:

“Chloroplast ribosome release factor 1 (AtcpRF1) is

essential for chloroplast development”, Plant Mol. Biol.,

64, 481-497 (2007).*

Fujita M, Mizukado S, Fujita Y, Ichikawa T, Nakazawa

M, Seki M, Matsui M, Yamaguchi-Shinozaki K,

Shinozaki K.: “Identification of stress-tolerance-related

transcription-factor genes via mini-scale Full-length

cDNA over-eXpressor (FOX) gene hunting system”,

Biochem. Biophys.Res.Commun. 364, 250-257 (2007).*

Ukitsu H.*, Kuromori T.*, Toyooka K., Goto Y.,

Matsuoka K., Sakuradani E., Shimizu S., Kamiya

A., Imura Y., Yuguchi M., Wada T., Hirayama T.,

Shinozaki K.　(* equally contribution) “Cytological and

Biochemical Analysis of COF1, an Arabidopsis Mutant

of an ABC Transporter Gene”. Plant Cell Physiol., 48,

1524-1533 (2007).*

Ito T., Nagata N., Yoshiba Y., Ohme-Takagi M., Ma H.

Shinozaki K.: “The Arabidopsis MALE STERILITY1

gene encoding a PHD-type transcription factor regulates

pollen and tapetum development”, Plant Cell, 19, 3549-

3562 (2007).*

Miwa K., Takano J., Omori H., Seki M., Shinozaki

K. Fujiwara T.: “Plants tolerant of high boron levels”,

Science, 318, 1417 (2007).*

Tran L-S. P., Urao T., Qin F., Maruyama K., Kakimoto

T., Shinozaki K. Yamaguchi-Shinozaki K.: “Functional

32.

33.

34.

35.

36.

37.

38.

39.

40.

41.

42.

43.

44.

analysis of AHK1/ATHK1 and cytokinin receptor

histidine kinases in response to abscisic acid, drought,

and salt stress in Arabidopsis”, Proc. Natl. Acad. Sci.

USA., 104, 20623-20628 (2007).*

Sakurai T., Plata G., Rodriguez-Zapata F., Seki M.,

Salcedo A., Toyoda A., Tohme J., Sasaki Y., Shinozaki K.

Ishitani M.: “Sequencing analysis of 20,000 full-length

cDNA clones from cassava reveals lineage specific

expansions in gene families related to stress response”,

BMC Plant Biology, 7, 66-84 (2007).*

Nanjo T., Sakurai T., Totoki Y., Toyoda A., Nishiguchi

M., Kado T., Igasaki T., Futamura N., Seki M., Sasaki

Y., Shinozaki K. Shinohara K.: “Functional annotation

of 19,841 Populus nigra full-length enriched cDNA

clones”, BMC Genomics, 8, 448-447 (2007).*

Sasaki Y., Takahashi K., Oono Y., Seki M., Yoshida R.,

Shinozaki K. Uemura M.:” Characterization of growth-

phase-specifi c responses to cold in Arabidopsis thaliana

suspension-cultured cells”, Plant Cell Environ., 31, 354-

365 (2008).*

Sakai T., vd Honing H., Nishioka M., Uehara Y.,

Takahashi M., Fujisawa N., Saji K., Seki M., Shinozaki

K., Jones MA., Smirnoff N., Okada K. Wasteneys

GO: “Armadillo repeat-containing kinesins and a

NIMA-related kinase are required for epidermal-cell

morphogenesis in Arabidopsis”, Plant J., 53, 157-171

(2008).*

Garcia M., Myouga F., Takechi K., Sato H., Nabeshima

K., Nagata N., Takio S., Shinzoaki K. Takano H.: “An

Arabidopsis homolog of the bacterial peptidoglycan

synthesis enzyme MurE has an essential role in

chloroplast development”, Plant J., 53, 924-934 (2008).*

Yoshida T., Sakuma Y., Todaka D., Maruyama K.,

Qin F., Mizoi J., Kidokoro S., Fujita Y., Shinozaki K.

Yamaguchi- Shinozaki K.: “Functional analysis of an

Arabidopsis heat-shock transcription factor HsfA3 in

the transcriptional cascade downstream of the DREB2A

stress-regulatory system”, Biochem. Biophys. Res.

Commun., 368, 515-521 (2008).*



― 196 ―

Kuromori T., Azumi Y., Hayakawa S., Kamiya A., Imura

Y., Wada T. Shinozaki K.: “Homologous chromosome

pairing is completed in crossover defective atzip4

mutant”, Biochem. Biophys.Res.Commun., 370, 98-103

(2008).*

Cartagena Joyce A., Matsunaga S., Seki M., Kurihara

D., Yokoyama M., Shinozaki K., Fujimoto S., Azumi

Y., Uchimiya S. Fukui K.: “The Arabidopsis SDG4

contributes to the regulation of pollen tube growth by

methylation of histone H3 lysines 4 and 36 in muture

pollen”, Dev. Biol., 315, 355-368 (2008).*

Shimizu H., Peng L., Myouga F., Motohashi R.,

Shinozaki K. Shikanai T.: “CRR23/NdhL is a subunit

of the chloroplast NAD(P)H dehydrogenase complex in

Arabidopsis”, Plant Cell Physiol., 49, 835-842 (2008).*

Qin F., Sakuma Y., Tran L-S., Maruyama K., Kidokoro S.,

Fujita Y., Fujita M., Umezawa T., Sawano Y., Miyazono

K., Tanokura M., Shinozaki K. Yamaguchi-Shinozaki K.:

“Arabidopsis DREB2A-interacting proteins function as

RING E3 ligases and negatively regulate plant drought

stress-responsive gene expression”, Plant Cell, 20, 1693-

1707 (2008).*

Mochida K., Saisho D., Yoshida T., Sakurai T. Shinozaki

K.: “TriMEDB: A database to integrate transcribed

markers and facilitate genetic studies of the tribe

Triticeae”, BMC Plant Biol., 8, 72 (2008)

Yasuda M., Ishikawa A., Jikumaru Y., Seki M., Umezawa

T., Asami T., Maruyama-Nakashita A., Kudo T.,

Shinozaki K., Yoshida S. Nakashita H.: “Antagonistic

interaction between syntemic acquired resistance and

the abscisic acid-mediated abiotic stress response in

Arabidopsis”, Plant Cell, 20, 1678-1692 (2008).*

Goda H., Sasaki E., Akiyama K., Maruyama-Nakashita

A., Nakabayashi K., Li W., Ogawa M., Yamauchi Y.,

Prestpm J., Aoki K., Kiba T., Takatsuto S., Fujioka S.,

Asami T., Nakano T., Kato H., Mizuno T., Sakakibara

H., Yamaguchi S., Nambara E., Kamiya Y., Takahashi

H., Yokota Hirai M., Sakurai T., Shinozaki K., Saito K.,

45.

46.

47.

48.

49.

50.

51.

52.

Yoshida S. Shimada Y.: “The AtGenExpress hormone-

and chemical-treatment data set: Experimental design,

data evaluation, model data analysis, and data access”,

Plant J., 55, 526-542 (2008).*

Endo A., Sawada Y., Takahashi H., Okamoto M., Ikegami

K., Koiwai H., Seo M., Toyomasu T., Mitsuhashi W.,

Shinozaki K., Nakazono M., Kamiya Y., Koshiba

T. Nambara E.: “Drought induction of Arabidopsis

9-cis-epoxycarotenoid dioxygenase occurs in vascular

parenchyma cells”, Plant Physiol., 147, 1984-1993

(2008).*

Yamaguchi-Shinozaki K. Shinozaki K.: “Transcriptional

regulatory networks in cellular responses and tolerance to

dehydration and cold stresses”, Annual Review of Plant

Biology, 57, 781-803 (2006).

Umezawa T., Fujita M., Fujita Y., Yamaguchi-Shinozaki

K. Shinozaki K.: “Enginieering drought tolerance in

plants: discovering and tailoring genes to unlock the

future”, Curr.Opin.Biotech., 17, 113-122 (2006).*

Shinozaki K. Yamaguchi-Shinozaki K.: “Global

analysis of gene networks to solve complex abiotic

stress responses, Cold Hardiness in Plants; Molecular

Genetics, Cell Biology and Physiology”, Proceeding

of 7th Internationa Plant Cold Hardiness Seminar in

Sapporo Japan, Edited by T.H.H. Chen, M. Uemura and S.

Fujikawa, CABI Publishing, 1-10 (2006).

Taji T., Takahashi S. Shinozaki K.: “Inositols and their

metabolites in abiotic and biotic stress responses”,

Subcellular Biochemistry Vol 39 on Biology of Inositols

and Phosphoinositides, Edetid by Arun Lahiri Majumder

and B.B. Biswas, 239-264 (2006).

Seki,M., Kamiya, A., Carninci, P., Hayashizaki, Y.

Shinozaki, K.: “Generation of full-length cDNA libraries

from plant materials”. In "Methods in Molecular

Biology-ESTs (Edited by Dr. John Parkinson)". Humana

Press Inc., NJ.in press (2006).

1.

2.

3.

4.

5.

【総説】



― 197 ―

Fujita M., Fujita Y., Noutoshi Y., Takahashi F., Narusaka

Y., Yamaguchi-Shinzoaki K. Shinozaki K.: “Crosstalk

between abiotic and biotic stress responses: a current

view from the points of convergence in the stress

signaling networks”, Curr.Opin. Plant Biol. 9, 436-442

(2006).*

Yamaguchi-Shinozaki K., Sakuma Y., Ito Y. Shinozaki

K.: “The DRE/DREB Regulon of gene expression in

Arabidopsis and rice in response to drought and cold

stress”, Drought Adaptation in Cereals, Edted by Jean-

Marcel Ribaut, Food Products Press, 583-597 (2006).

Seki M., Umezawa T., Kim J.M., Matsui A., To T.

Shinozaki K.: “Transcriptome analysis of plant drought

and salt stress response”, Advances in Molecular

Breeding toward Drought and Salt Tolerant Crops, Edited

by Drs. M.A. Jenks, P.M. Hasegawa and S.M. Jain.,

Springer Publishing. 261-283 (2007).

Nakashima K., Todaka D., Ahmed S., Umezawa T., Seki

M., Shinozaki K. Yamaguchi-Shinozaki K.:“Molecular

analysis of abiotic stress: Tolerant mechanisms in

soybean and its application to breeding”, JIRCAS

Working Report, 51, 13-19 (2007).

Shinozaki K., Yamaguchi-Shinozaki K.: “Gene networks

involved in drought stress response and tolerance”. J.

Exp Bot. 58, 221-227 (2007).*

Seki M., Umezawa T., Urano K. Shinozaki K.:

“Regulatory metabolic networks in drought stress

responses”, Curr.Opin. Plant Biol. 10, 296-302 (2007).*

Tran L-S. P., Nakashima K., Shinozaki K. Yamaguchi-

Shinozaki K.: “Plant gene networks in osmotic stress

response: from genes to regulatory networks”, Methods

in Enzymology, 428, 109-128 (2007).

Hirayama T. Shinozaki K.: “Perception and transduction

of abscisic acid signals: keys to the function of the

versatile plant hormone ABA”, Trends in Plant Sci. 12,

343-351 (2007).*

6.

7.

8.

9.

10.

11.

12.

13.

14.

15.

16.

17.

18.

19.

20.

Shinozaki K.: “Acceleration of soybean genomics using

large collection of DNA markers for gene discovery”,

DNA Res., 14, 235 (2008).

中島一雄 , 篠崎一雄 , 篠崎和子： “環境耐性作物開

発のための制御因子の探索と分子育種への利用 (1)

レギュロンバイオテクノロジーを利用した環境スト

レス耐性作物の開発の現状と展望 ”, ブレインテク

ノニュース , 117, 1-7 (2006)

片桐健 , 梅澤泰史 , 篠崎和子 , 篠崎一雄： “環境耐

性作物開発のための制御因子の探索と分子育種へ

の利用 (2) アブシジン酸の制御因子 , シグナル伝達

因子を用いたネットワーク制御による環境ストレ

ス耐性の付与 ”, ブレインテクノニュース , 117, 8-13

(2006)

梅澤泰史 , 浦野薫 , 篠崎一雄： “乾燥ストレス応答

におけるシグナル伝達と耐性獲得の分子機構 ”, 蛋

白質核酸酵素別冊「植物における環境と生物ストレ

スに対する応答」, 島本功 , 篠崎一雄 , 白須賢 , 篠崎

和子編 , 共立出版 , 550-556 (2007).

黒森崇 , 和田拓治 , 篠崎一雄： “Saturation

mutagenesisが可能なシロイヌナズナにおける遺伝

子破壊系統の収集とフェノーム解析 1”, 「生物の科

学遺伝」, 別冊 No.21, 104-107 (2007).

黒森崇 , 明賀史純 , 篠崎一雄： “シロイヌナズナの

全遺伝子解明に向けての遺伝子破壊型変異体整備と

表現型解析 ”, 実験医学「ゲノム情報と生命現象の

統合的理解 2007」, 榊佳之 , 伊藤隆司 , 辻省次 , 小原

雄治編 , 洋土社 , 25, 55-60 (2007).

伊藤卓也 , 篠崎一雄： “花粉制御への一歩 - 花粉成

熟に必須なシロイヌナズナMSI転写因子の同定 ”,

ブレインテクノニュース , 127, 7-11 (2008).



― 198 ―

Shinzoaki K. and Yamaguchi-Shinozaki K.: Molecular

responses and tolerance to drought stress in higher plants,

The First International Conference on the Theory and

Plactices in Biological Water Saving, May 21-25, Beijing

China (2006).

Shinozaki K. and Yamaguchi-Shinozaki K.: Gene

networks involved in drought stress responses and

tolerance, Bio Vision Alexandira 2006, Apri.26-29,

Alexandria Egypt (2006).

Shinozaki K. and Yamaguchi-Shinzoaki K.: Signaling

and transcription networks in drought stress response,

Keystone Symposia on Plant Responses to Abiotic Stress,

April 8-13, Colorado USA (2006).

Seki M., Ishida J., Morosawa T., Matusi A., Kim

J.M., Nakajima M., Enju A., To T., Iida K., Go M.,

Mochizuki Y., Hasegawa Y., Toyoda T. and Shinzoaki

K.: “Arabidopsis whole-genome transcriptome analysis

under abiotic stress conditions”, The 16th Penn State

Symposium in Plant Physiologyon RNA Biology: Novel

Insights from Plant Systems, May 18-20, Penn State

USA (2006).

Ito T., Nagata N., Ohme-Takagi M., Ma H. and

Shinozaki K.: An Arabidopsis MALE STERILITY1 gene

encoding a PHD-type transcription factor regulates early

microgametogenesis, The 16th Penn State Symposium in

Plant Physiology on RNA Biology: Novel Insights from

Plant Systems, May 18-20, Penn State USA (2006).

Hirayama T., Tian C., Tsuboi Y., Chikayama E.,

Sekiyama Y., Kuromori T., Shinozaki K. and Kikuchi J.:

Advances in hetero-nuclear NMR-based metabolomics

approach in plant systems, 20th IUBMB Internationa

Congress of Biochemistry and Molecular Biology and

11th FAOBMB Congress, June 18-23, Kyoto Japan

(2006).

Tsuboi Y., Hirayama T., Shinozaki K. and Kikuchi J.:

Toward an NMR-based nutrigenomics assisted by stable

研究発表（学会発表）

1.

2.

3.

4.

5.

6.

7.

【国際会議】

8.

9.

10.

11.

12.

13.

isotope labeling of plants through animals, 20th IUBMB

Internationa Congress of Biochemistry and Molecular

Biology and 11th FAOBMB Congress, June 18-23,

Kyoto Japan (2006).

Hirayama T., Tsuboi Y., Shinozaki K. and Kikuchi

J.: Toward an NMR-based nutrigenomics assisted

by stable isotope labeling of plants through animals,

Metabolomics 2006 on the Second Scientific Meeting

of the Metabolomics Society, June 24-29, Boston USA

(2006).

Tian C., Tsuboi Y., Chikayama E., Sekiyama Y.,

Kuromori T., Shinozaki K., Hirayama T. and Kikuchi J.:

An effi cient strategy for the metabolic characterization of

Arabidopsis mutants with visible and silent phenotypes

by fast 1D-NMRscreening followed multi-dimensional

NMR analysis, Metabolomics 2006 on the Second

Scientifi c Meeting of the Metabolomics Society, June 24-

29, Boston USA (2006).

Ichikawa T., Nakazawa M., Kondou Y., Ishikawa A.,

Kawashima M., Iizumi H., Seki M., Fujita M., Muto S.,

Shinozaki K. and Matsui M.: Evaluation of Arabidopsis

FOX line: T2 phenotype and phenotype recapitulation

by transformation, 17th International Conference on

Arabidopsis Research, June 28- July 2, Wisconsin-

Madison USA (2006).

Ichimura K., Casais C., Peck S., Shinozaki K. and

Shirasu K.: MEKK1 negatively regulates tissue specifi c

and temperature dependent cell death in Arabidopsis,

17th International Conference on Arabidopsis Research,

June 28- July 2, Wisconsin-Madison USA (2006).

Katagiri T. and Shinozaki K.: Functional analysis of

phosphatidic acid during germination, 17th International

Conference on Arabidopsis Research, June 28- July 2,

Wisconsin-Madison USA (2006).

Motohashi R., Okada E., Kuroda H., Nagata N., Matsui

M. and Shinozaki K.: Functional analysis of ribosome



― 199 ―

binding factor (RBFA) homologue using Ac/Ds

transposon system in Arabidopsis thaliana. Plant Biology

2006, Aug. 5-9, Boston USA (2006).


Shinozaki K. and Hirayama T.: Characterization of novel

ABA hypersensitive mutant, ahg1,Plant Biology 2006,

Aug. 5-9, Boston USA (2006).

Hirayama T., Tian C., Tsuboi Y., Chikayama E.,

Sekiyama Y., Kuromori T., Shinozaki K. and Kikuchi J.:

Adcances in hetero-nuclear NMR-based metabolomics

approach in plant systems, Plant Biology 2006, Aug. 5-9,

Boston USA (2006).

Hayashi S., Kuromoti T., Shinozaki K. and Hirayama T.:

Analysis of an Arabidopsis root hair defective mutant

shv3, Plant Biology 2006, Aug. 5-9, Boston USA (2006).

Yoshida T., Nishimura N., Kitahata N., Kuromori

T.,Kuroda H., Matsui M., Asami T., Shinozaki K. and

Hirayama T.: ABA-hypersensitive germination3 encodes

a protein phosphatase 2C (AtPP2CA) that strongly

regulates abscisic acid signaling during germination

among Arabidopsis protein phosphatase 2Cs, Plant

Biology 2006, Aug. 5-9, Boston USA (2006).

Shinozaki K. and Yamaguchi-Shinozaki K.: Regulatory

gene networks in drought stress responses and tolerance,

Tropical Crop Biotechnology Conference 2006, Aug. 16-

19, Cairns Queensland Australia (2006).

Shinzoaki K. and Yamaguchi-Shinozaki K.: Regulatory

networks in drought stress response and tolerance, 8th

International Congress of Plant Molecular Biology, Aug.

20-25, Adelaide Australia (2006).

Seki M., Ishida J., Morosawa T., Matsui A., Kim J.M.,

Nakajima M., Enju A., To T., Iida K., Go M., Mochizuki

Y., Hasegawa Y., Kaminuma E., Toyoda T. and Shinozaki

K.: Arabidopsis whole-genome expression profi ling under

abiotic stress conditions, 8th International Congress of

Plant Molecular Biology, Aug. 20-25, Adelaide Australia

(2006).

14.

15.

16.

17.

18.

19.

20.

21.

22.

23.

24.

25.

26.

Myouga F., Akiyama K., Sakurai T., Motohashi R. and

Shinozaki K.: Gene discovery for chloroplast proteins

involved in drought tolerance in Arabidopsis using

the rarge database, 8th International Congress of Plant

Molecular Biology, Aug. 20-25, Adelaide Australia

(2006).

Yasuda M., Ishikawa A., Asami T., Kamiya Y., Shinozaki

K., Kudo T., Yoshida S. and Nakashita H.: Antagonistic

interaction between environmental stress response

and systemic acquired resistance in Arabidopsis, 8th



Kim J.M., Kuromori T., To T., Hirayama T., Seki M.

and Shinozaki K.: Functional analysis of AtORC1a in a

phase progression, 8th International Congress of Plant

Molecular Biology, Aug. 20-25, Adelaide Australia

(2006).

Nakamura H., Hakata M., Kajikawa M., Ando S.,

Amano K., Toki N., Higashi N., Fujita M., Enju A., Seki

M., Nakazawa M., Ichikawa T., Matsui M., Shinzoaki

K., Miyao A., Toki T., Hirochika H. and Ichikawa H.:

Genome-wide functional analysis of rice genes using

FOX hunting system 1. Overview and current status, 8th



Hakata M., Nakamura H., Asano T., Kajikawa M.,

Amano K., Toki N., Higashi N., Fujita M., Enju A.,

Seki M., Nakazawa M., Ichikawa T., Shinozaki K.,

Matsui M., Komatsu S., Miyao A., Toki S., Hirochika

H. and Ichikawa H.: Genome-wide functional analysis

of rice genes using FOX hunting system 2. Analysis and

identifi caiton of introduced cDNAs in the FOX-rice lines,

8th International Congress of Plant Molecular Biology,

Aug. 20-25, Adelaide Australia (2006).

Ichikawa H., Nakamura H., Hakata M., Kajikawa M.,

Ando S., Amano S., Toki N., Higashi N., Fujita M.,

Enju A., Seki M., Nakazawa M., Ichikawa T., Matsui

M., Shinozaki K., Miyao A., Toki T. and Hirochika H.:

FOX hunting system: a genome-wide functional analysis



― 200 ―

of rice genes using transgenic plants, 8th International

Congress of Plant Molecular Biology WorkShop, Aug.


Urano K., Maruyama K., Takeda M., Suzuki H., Saito

K., Shibata D., Yamaguchi-Shinozaki K. and Shinozaki

K.: Analysis of ABA-dependent metabolic networks

in drought stress response, 2006 Gordon Research

Conference on Salt & Water Stress in Plants, Sep. 3-8,

Oxford UK (2006).

Tran L-S., Urao T., Qin F., Osakabe Y., Kakimoto T.,

Shinozaki K. and Yamaguchi-Shinozaki K.: Functional

analyses of AtHK1, AHK2, AHK3 and AHK4/CRE1

in response to abiotic stresses, 2006 Gordon Research

Conference on Salt & Water Stress in Plants, Sep. 3-8,

Oxford UK (2006).

Fujita Y., Fujita M., Satoh R., Maruyama K., Sayama

H., Seki M., Hiratsu K., Ohme-Takagi M., Shinzoaki K.

and Yamaguchi-Shinozaki K.: AREB1 mediates novel

ABRE-dependent ABA-signaling that enhances drought

stress tolerance in Arabidopsis thaliana, 2006 Gordon

Research Conference on Salt & Water Stress in Plants,

Sep. 3-8, Oxford UK (2006).

Shinozaki K.: Functional genomics in RIKEN Plant

Science Center for the improvement of plant productivity,

PKU-CCAST-RIKEN Joint Symposium-Life Science

Panel, Nov. 10, Beijing China (2006).

Yamaguchi-Shinozaki K. and Shinozaki K.: Regulatory

networks of gene expression in environmental stress

response, GARNet 2006, Sep 11-12, Bristol UK (2006).

Nakashima K., Tran L-S Phan., Dong NV., Fujita M.,

Maruyama K., Todaka D., Ito Y., Shinozaki K. and

Yamaguchi-Shinozaki K.: Functional analysis of NAC-

type transcription factor OsNAC6 involved in abiotic

stress-responsive gene expression in rice, 4th Internationa

Rice Functional Genomics Symposium, Oct. 9-11,

Montpellier France (2006).

Umezawa T., Todaka D., Totoki Y., Sakurai T., Seki M.,

27.

28.

29.

30.

31.

32.

33.

34.

35.

36.

37.

38.

39.

Toyoda A., Maruyama K., Nakashima K., Mizukado S.,

Kasuga M., Ahmed S, Sado H., Harada K., Tsubokura Y.,

Sato S., Anai T., Ishimoto M., Funatsuki H., Teraishi M.,

Shinano T., Akashi R., Sakaki Y., Yamaguchi-Shinozaki

K. and Shinozaki K.: Construction of a soybean full-

length cDNA library and oligonucleotide microarray, The

KAZUSA Conference on legume Genetics and Genomics

in Asia, Nov. 27-28, Kisarazu Japan (2006).

Todaka D., Nakashima K., Ito Y., Ohme-Takagi M.,


analysis of the OsPIF1 gene down-regulated by drought

stress in rice. ICGEB Meeting: Biotic and Abiotic Stress

Responses in Plants, Dec 11-13, New Delhi, India. (2006)

.

Qin F., Sakuma Y., Tran L-S P., Osakabe Y., Shinozaki

K. and Yamaguchi-Shinzoaki K.: The function and

regulation of an Arabidopsis transcription factor

DREB2A in plant water deficit and high temperature

stresses, Gordon Research Conferences on Temperature

Stress in Plants, Jan 21-26, Ventura CA (2007).

Osakabe Y., Mizuno S, Shinozaki K. and Yamaguchi-

Shinozaki K.: Functional analysis of a leucine-rich

repeat receptor like kinase, RPK1, involved in ABA

signal transduction of Arabidopsis, Gordon Research

Conferences on Temperature Stress in Plants, Jan 21-26,

Ventura CA (2007).

Ito Y., Shinozaki K. and Yamaguchi-Shinozaki K.:

Comprehensive analysis of 10 DREB/CBF family genes

in rice, Gordon Research Conferences on Temperature

Stress in Plants, Jan 21-26, Ventura CA (2007).

Kidokoro S., Maruyama K., Nakashima K., Sakuma

Y., Imura Y., Shinozaki K. and Yamaguchi-Shinozaki

K.: Transcriptional regulation of the DREB1C gene

in response to low temperature, Gordon Research

Conferences on Temperature Stress in Plants, Jan 21-26,

Ventura CA (2007).

Seki M., Matsui A., Kim J-M., Ishida J., Nakajima

M., Morosawa T., Kawashima M., Satou M., Kim T.,



― 201 ―

40.

41.

42.

43.

44.

45.

Kurihara Y., Kaminuma E., Endo T., Mochizuki Y.,

Kobayashi N., Toyoda T. and Shinozaki K.: Arabidopsis

whole-genome transcriptome analysis under drought,

cold, high-salinity, and ABA treatment conditions

using tiling array and 454 sequencing technology, 24th

Symposium in Plant Biology on Gene Silincing: The

Biology of Small RNAs and the Epigenome, Jan. 18-20,

Riverside USA (2007).

Shinozaki K.: Functional genomics in drought stress

responses, CINA-JAPAN Joint Workshop on Plant

Molecular Breeding, Mar 12-13, Beijing China (2007).

Shinozaki K.: Molecular biology and salinity stress

responses and tolerance, BIOTEC-RIKEN Research

Forum, May 22-25, Thailand (2007).

Shinozaki K., Yamaguchi-Shinozaki K. and Seki M.:

Regulatory gene network in drought and ABA responses.,

18th International Conference on Arabidopsis Research.,

June 20-23., Beijing China (2007).

Mitsuda N., Iwase A., Yamamoto H., Yoshida M.,

Seki M., Shinozaki K. and Ohme-Takagi M.: NAC

transcription factors NST1, NST2 and NST3 are key

regulators of secondary wall formation in Arabidopsis,

18th Internat ional Conference on Arabidopsis

Research.,June 20-23., Beijing China (2007).

Ichikawa T., Nakazawa M., Kawashima M., Iizumi H.,

Kuroda H., Kondou Y., Ishikawa A., Seki M., Fujita

M., Higuchi M., Shinozaki K. and Matsui M.: The Fox

hunting system: an alternative gain-of- function gene

hunting technique, 18th International Conference on

Arabidopsis Research.,June 20-23., Beijing China (2007).

Seki M., Matsui A., Kim J-M., Ishida J., Nakajima

M., Morosawa T., Kawashima M., Satou M., Kim T.,

Kurihara Y., Kaminuma E., Endo T., Mochizuki Y.,

Kobayashi N., Toyoda T. and Shinozaki K.: Arabidopsis

whole-genome transcriptome analysis under drought,

conditions using tiling array and 454 sequencing

technology, 18th International Conference on Arabidopsis

Research.,June 20-23., Beijing China (2007).

46.

47.

48.

49.

50.

51.

52.

Kuromori T., Kamiya A., Ito T., Wada T., Hirayama T.

and Shinozaki K.: A mutant-based phenome analysis

and collection of mutant phenotype information, 18th

International Conference on Arabidopsis Research.,June

20-23., Beijing China (2007).

Myouga F., Iizumi H., Kuromori T., Motohashi R. and

Shinozaki K.: Characterization of Ds-tagged mutants for

nuclear-encoded chloroplast protein involved in plastid

development and oxi-dative stress responses, 18th



Hirayama T., Narusaka Y., Yasuda M., Narusaka M.,

Hayashi S., Kitahata N., Kuromori T., Asami T. and

Shinozaki K.: Impairment in the mRNA degradation

causes abnormal response to both abscisic acid and

salicylic acid in Arabidopsis, 18th International

Conference on Arabidopsis Research.,June 20-23.,

Beijing China (2007).

Urano K., Maruyama K., Takeda M., Sakurai N., Suzuki

H., Saito K., Shibata D., Yamaguchi-Shinozaki K. and

Shinozaki K.: Comprehensive analysis of ABA-regulated

metabolome and transcriptome in drought response

of Arabidopsis, 18th International Conference on

Arabidopsis Research.,June 20-23., Beijing China (2007).

Takahashi F., Yoshida R., Ichimura K., Mizoguchi T.,

Yonezawa M., Maruyama K., Yamaguchi-Shinozaki K.

and Shinozaki K.: The MAP kinase cascade MKK3-

MPK6 is an important part of the jasmonate signal

transduction pathway in Arabidopsis, 18th International

Conference on Arabidopsis Research.,June 20-23.,

Beijing China (2007).

Garcia M., Myouga F., Takechi K., Sato H., Nabeshima

K., Nagata N., Takio S., Shinozaki K. and Takano H.:

An Arabidopsis chloroplast-targeting MurE homolog

has an essential role in chloroplast development, 18th






― 202 ―

gene networks in drought and ABA responses, IPGSA

19th Annual Meeting, July21-25, Puerto Vallarta Mexico

(2007).

Ayano M., Godal H., Shinozaki K., Watanabe N.,

Yokoyama M. and Yoshida S.: An oxylipin, (12Z, 15Z)-

9-hydroxy-10-oxo octadecadienoic acid [KODA] shows

unique signaling on fl oral development of higher plants,

IPGSA 19th Annual Meeting, July21-25, Puerto Vallarta

Mexico (2007).

Ayano M., Godal H., Shinozaki K., Watanabe N.,

Yokoyama M. and Yoshida S.: Function analysis of

oxylipin, 9-hydroxy-10-oxo12(z), 15(z)-octadecadienoic

acid (KODA) in Arabidopsis thaliana, IPGSA 19th

Annual Meeting, July21-25, Puerto Vallarta Mexico

(2007).


Shinozaki K. and Hirayama T.: ABA-hypersensitive

germination encodes a protein phosphatase 2C, an

essential component of abscisic acid signaling in

Arabidopsis seed, IPGSA 19th Annual Meeting, July21-

25, Puerto Vallarta Mexico (2007).

Goda H., Yoshida S., Shinozaki K. and Shimada Y.:

Finding of a novel hormone-related compound by using

AtGenExpress microarray data, IPGSA 19th Annual

Meeting, July21-25, Puerto Vallarta Mexico (2007).


gene network in drought stress response, 2nd World

Conference of Stress, August 23-26, Butapest Hungary

(2007).

Yamaguchi-Shinozaki K. and Shinozaki K.: Improving

abiotic stress-tolerance in crops,Frontiers in Transgenesis,

Sep 26-28, St Louis USA (2007).

Yamaguchi-Shinozaki K. and Shinozaki K.: Regulatory

networks of gene expression in abiotic stress responses in

Arabidopsis and rice, 2007 International Symposium and

Annual Meeting of the KSABC, Oct 11-13, Gyeongiu

Korea, (2007).

53.

54.

55.

56.

57.

58.

59

60.

61.

62.

63.

64.

65.

66.

Matsukura S., Yoshida T., Todaka D., Ito Y., Maruyama

K., Shinozaki K. and Yamaguchi-Shinozaki K.:

Functional analysis of the OsDREB2B gene encoding

a rice transcription factor involved in abiotic-stress-

responsive gene expression, 2007 International

Symposium and Annual Meeting of the KSABC, Oct 11-

13, Gyeongiu Korea, (2007).

Nakashima K., Tran L-S Phan., Dong NV., Fujita M.,


K. and Yamaguchi-Shinozaki K.: Functional analysis

of NAC-type transcription factor OsNAC6 involved in

abiotic stress-responsive gene expression in rice, 2007

International Symposium and Annual Meeting of the

KSABC, Oct 11-13, Gyeongiu Korea, (2007).

Yamada K., Osakabe Y., Maruyama K., Fujita Y., Mizoi J.,


analysis of osmotic stress-inducible sugar transporter

gene in Arabidopsis. 2007 International Symposium and


Korea, (2007).

Ito Y., Maruyama K., Shinozaki K. and Yamaguchi-

Shinozaki K.: Comprehensive analysis of DREB1/CBF

family genes in rice, 2007 International Symposium and


Korea, (2007).

Yamaguchi-Shinozaki K. and Shinozaki K.: Improving

transgenic tolerance to abiotic stresses including

drought in rice, The 5th International Symposium of rice

functional genomics,Oct. 15-17, Tsukuba Japan (2007).



analysis of the OsPIF1 gene down-regulated by drought

stress in rice.The 5th International Symposium of rice

functional genomics, Oct. 15-17, Tsukuba Japan (2007).

Ito Y., Maruyama K., Shinozaki K. and Yamaguchi-

Shinozaki K.: Comprehensive analysis of DREB1/CBF

family genes in rice, The 5th International Symposium

of rice functional genomics,Oct. 15-17, Tsukuba Japan

(2007).



― 203 ―

Matsukura S., Yoshida T., Todaka D., Ito Y., Maruyama

K., Shinozaki K. and Yamaguchi-Shinozaki K.:

Functional analysis of the OsDREB2B gene encoding

a rice transcription factor involved in abiotic-stress-

responsive gene expression,The 5th International

Symposium of rice functional genomics, Oct. 15-17,

Tsukuba Japan (2007).

Nakashima K., Tran L-S Phan., Nguyen D.V., Fujita M.,


K. and Yamaguchi-Shinozaki K.: Functional analysis of

a NAC-type transcription factor OsNAC6 involved in

abiotic and biotic stress-responsive gene expression in

rice, The 5th International Symposium of rice functional

genomics, Oct. 15-17, Tsukuba Japan (2007).


Gene network in drought stress response, 2007 Genomics

Symposium on The Genomics of Drought, Oct 22-24,

Adelaide Australia (2007).

Shinozaki K.: Plant functional genomics in drought stress

response, National Tipei University Special Seminar, Oct

15-18, Taipei China (2007).

Seki M.: Arabidopsis whole-genome transcriptome

analysis under drought, cold, high-salinity, and

ABA treatment conditions using tiling array and 454

sequencing technology, 2007 Iwate International

Symposium on Biological Sciences --The Impacts of

OMICS--, Oct. 29, Morioka Japan (2007).


Plant functional genomics in drought stress response,

The 6th Asian Crop Science Association Conference and

The 2nd International Conference on Rice for the Future,

Nov. 5-9, Bangkok Thailand (2007).

Yamaguchi-Shinozaki K. and Shinozaki K.: Regulation

of gene expression in response to abiotic stresses and

improving stress tolerance in crops, Agricultural Plant

Stress Response VII on abiotic stress and Plant Growth

and Development, Nov. 8, Gwangju Korea (2007).

67.

68.

69.

70.

71.

72.

73.

Arinaga N., Osakabe Y., Yamada K., Maruyama K.,


analysis of an osmotic stress-inducible potassium

transporter, KUP6, in Arabidopsis, Agricultural Plant

Stress Response VII on abiotic stress and Plant Growth

and Development, Nov. 8, Gwangju Korea (2007).

Maruyama K., Takeda M., Sakurai N., Kidokoro S.,

Yamada K., Suzuki H., Saito K., Shinozaki K. and

Yamaguchi-Shinozaki K.: A comparative analytic

approach to the chilling/drought metabolites and

transcripts: DREB1A/CBF3 and chloroplastic raffinose

synthase playing a key role in low-temperature condition,

Agricultural Plant Stress Response VII on abiotic stress

and Plant Growth and Development, Nov. 8, Gwangju

Korea (2007).

Kanamori N., Fujita Y., Yoshida T., Sayama H., Fujita

M., Maruyama K., Hiratsu K., Ohme-Takagi M.,

Seki M., Shinozaki K. and Yamaguchi-Shinozaki K.:

Molecular characterization of AREB family transcription

factors involved in ABA-responsive gene expression in

Arabidopsis thaliana, Agricultural Plant Stress Response

VII on abiotic stress and Plant Growth and Development,

Nov. 8, Gwangju Korea (2007).

Osakabe Y., Mizuno S., Maruyama K., Osakabe K.,


analysis of a leucine-rich repeat receptor like kinase,

RPK1, involved in ABA signal transduction of

Arabidopsis, Agricultural Plant Stress Response VII on

abiotic stress and Plant Growth and Development, Nov. 8,

Gwangju Korea (2007).



analysis of the OsPIFI gene down-regulated by drought

stress in rice, Agricultural Plant Stress Response VII on

abiotic stress and Plant Growth and Development, Nov. 8,

Gwangju Korea (2007).

Yoshida T., Sakuma Y., Todaka D., Osakabe Y.,

Maruyama K., Shinozaki K. and Yamaguchi-Shinozaki

K.: Functional analysis of Arabidopsis HsfA3 controlled

74.

75.

76.

77.

78.

79.



― 204 ―

by DREB2A regulatory system under heat stress,

Agricultural Plant Stress Response VII on abiotic stress

and Plant Growth and Development, Nov. 8, Gwangju

Korea (2007).

Fujita Y., Yamaguchi-Shinozaki K. and Shinozak K.:

Improving drought and salt stress tolerance in plants by

gene transfer, Fourth Biomass-Asia Workshop, Nov 20-

22, Selangor Darul Ehsan, Malaysia (2007).

Tanaka R., Nagane R., Myouga F., Motohashi R.,

Shinozaki K. and Tanaka A.: HPLC-based screening for

pigment metabolism mutants in Arabidopsis thaliana, 7th

International Conference on Tetrapyrrole Photoreceptors

in Photosynthetic Organisms, Dec. 9-14, Kyoto Japan

(2007).

Shinozaki K., Urano K., Maruyama K., Takeda M.,

Ogata Y., Sakurai N., Suzuki H., Saito K., Shibata D.,

Kobayashi M. and Yamaguchi-Shinozaki K.: Regulatory

gene network in drought-induced metabolic change,

5th International Conference on Plant Metabolomics,

Yokohama Japan, Jul 15-18 (2008).

Mochida K., Furuta T., Ebana K., Ogihara Y., Shinozaki

K and Kikuchi J: Metabolic phenotyping of genetic

resources in gramineae using NMR, 5th International

Conference on Plant Metabolomics, Yokohama Japan,

Jul 15-18 (2008).

Akiyama K., Chikayama E., Yuasa H., Shimada Y.,

Tohge T., Shinozaki K., Yokota-Hirai M., Sakurai T.,

Kikuchi J. and Saito K.: PRIME: The Platform for

RIKEN metabolomics to assist systems biology studies,

5th International Conference on Plant Metabolomics,


Sakurai T., Kondou Y., Akiyama K., Sakakibara H.,

Kusano M., Takahashi H., Mori M., Takatsuji H.,

Shinozaki K., Oda K., Hirochika H. and Matsui M.:

Construction of RIKEN rice FOX Arabidopsis mutant

line database, 5th International Conference on Plant

Metabolomics, Yokohama Japan, Jul 15-18 (2008).

80.

81.

82.

83.

84.

85.

86.

87.

Urano K., Maruyama K., Takeda M., Ogata Y., Sakurai

N., Suzuki H., Saito K., Shibata D., Kobayashi

M., Yamaguchi-Shinozaki K. and Shinozaki K.:

Characterization of the ABA-regulated global responses

to dehydration in Arabidopsis by metabolomics, 5th

International Conference on Plant Metabolomics,


Maruyama K., Kidokoro S., Takasaki H., Sakurai N.,

Suzuki H., Saito K., Shibata D., Shinozaki K. and

Yamaguchi-Shinozaki K.: A comparative analysis of

transcripts and metabolites in rice under chilling and

dehydration conditions, 5th International Conference on

Plant Metabolomics, Yokohama Japan, Jul 15-18 (2008).

篠崎連携研究グループ （機能開発研究チーム） -...

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篠崎連携研究グループ（機能開発研究チーム） -...