China (PRC) – Cabbage, Celery and Green Onion Chinese Cabbage - Brassica rapa L. pekinensis Celery - Apium graveolens L. Green Onion - Allium fistulosum L.

Pengzhou City Bureau of Agriculture Soil & Fertilizer Station Pengzhou, Chendu, Sichuan Province

This Agrostim study was conducted in China using three cultivar vegetable species in the following experimental design: A. Cultivar: Chinese Cabbage Species: Brassica rapa L. pekinensis (f. Cruciferae) B. Cultivar: Celery Species: Apium graveolens L. (f. Umbelliferae) C. Cultivar: Green Onion Species: Allium fistulosum L. (Alliaceae) Location: Pengzhou City, Chendu, Sichuan Province, China Dates: September 1996 to June 1997

2

Experimental Design: 3 Separate Treatments plus Control: A. Control: Conventional fertilizer application (NPK)

25 kilograms/mu 15:15:15 applied 3 times, plus 20 buckets of liquid farm manure applied 4 times

B. Agrostim: 1.5% (dilution in water) soil applied (root area) Applied 3 times plus Agrostim 1% foliar application C. Agrostim: 1% (dilution in water) foliar applied 3 times. D. Green Leaf Fertilizer (Organic Competitor of Agrostim): 1% (dilution in water) foliar applied 3 times.

Chinese Cabbage – Brassica rapa L. pekinensis

Study Results: 1. Soil Conditions & Previous Crop Yield:

The soil is alluvial, grey-brown clay loam soil for growing rice. Previous crop was rice. The growth was even, which indicates that the soil conditions are even (homogeneous) – soil quality – Average.

2. Test Plot design:

Each of the 4 general treatments was repeated 3 times for a total of 12 replicate samples. Each test plot was 20 m3 – Groups were separated by a cleared barrier 50 cm wide for ease of management and to ensure little or no fertilizer application spread to adjacent plots.

3. Test Plot management:

Seed sowing was made on October 6, 1996, and seedlings were transplanted on November 2, 1996, spacing 0.44m x 0.6m, equivalent to 2,500 plants per mu. 75 plant holes per plot. Crop harvested on March 20, 1997.

3

4. Observations:

Table 1. Comparison of Chinese cabbage Production Rates, Gross Weight Increase, and Percentage of Agrostim Yield Increases over the Control (A).

Treatment Production by plots Production Production I II III Ave. kg/mu Compared to A A 106.7 100.8 104.2 104.0 3,463.3 0 B 115.3 119.8 112.5 116.2 3,993.3 + 15.3% C 109.6 114.1 113.2 112.3 3,743.3 + 8.3% D 102.9 101.2 100.5 101.5 3,353.3 - 3.1% Treatments B & C are Significantly Greater than Treatment D and the Control (A) by Analysis of Variance at the 0.001 Level of Significance.

Fig.1. Chinese cabbage. Foliar applied Agrostim plants (B) are on the right side, Control (A) plants are on the left.

4

Celery – Apium graveolens L. Study Results: 1. Soil Conditions & Previous Crop Yield:

The soil is alluvial, gray-brown clay loam soil for growing rice. Previous crop was rice. The growth was even, which indicates that the soil conditions are even (homogeneous) – soil quality – Average.

2. Test Plot design:

Each of the 4 general treatments was repeated 3 times for a total of 12 replicate samples. Random arrangement of each group. Each test plot was 20 m3 – Groups were separated by a cleared barrier 50 cm wide for ease of management and to ensure little or no fertilizer application spread to adjacent plots.

3. Test Plot management:

Seed sowing was made on August 20, 1996, and seedlings were transplanted on October 14, 1996, spacing 8.1 x 22.1 centimeters, equivalent to 37,200 plants per mu. Crop harvested on January 6, 1997.

4. Observations: Table 2. Comparison of Celery Production Rates, Gross Weight Increase, and Percentage of Agrostim Yield Increases over the Control (A). Treatment Production by plots Production Production I II III Ave. kg/mu Compared to A A 87.6 93.2 100.0 93.6 3,354.2 0 B 106.4 100.4 103.2 103.5 3,712.5 +12.7% C 100.6 99.4 103.8 101.3 3,650.6 +8.8% D 107.1 103.6 105.8 105.5 3,511.0 +4.7% Production by plots - Treatments B, C & D are Significantly Greater than the Control (A) by Analysis of Variance at the 0.001 Level of Significance. Production by weight produced – Treatment B is Significantly Greater than Treatments C & D and the Control (A) and Treatment C is Significantly Greater than treatment D by ANOVA at the 0.01 Level of Significance. Note: Significant Differences are noted by separate colors.

5

Fig. 2. Individual Agrostim treated Celery plants (B) averaged 350 grams each whereas Control plants (A) weighed an average of 203 grams per individual plant.

Fig.3. Agrostim treated (B & C) provided Celery plants with a higher yield, increased green color, more favorable and more disease resistant than either the control or the competing organic fertilizer (D).

6

Green Onion – Allium fistulosum L.

Study Results: 1. Soil Conditions & Previous Crop Yield:

The soil is alluvial, gray-brown clay loam soil for growing rice. Previous crop was rice. The growth was even, which indicates that the soil conditions are even (homogeneous) – soil quality – Average.

2. Test Plot design:

Each of the 4 general treatments was repeated 3 times for a total of 12 replicate samples. Each test plot was 20 m3 – Groups were separated by a cleared barrier 50 cm wide for ease of management and to ensure little or no fertilizer application spread to adjacent plots.

3. Test Plot management:

Seed sowing was made on August 28, 1996, and seedlings were transplanted on October 7, 1996, spacing 30.0 x 9.3 centimeters, equivalent to 23,900 plants per mu. The onions were harvested on June 1, 1997, a growth period of 236 days.

4. Observations: Table 3. Comparison of Green Onion Production Rates, Gross Weight Increase, and Percentage

of Agrostim Yield Increases over the Control (A). Treatment Production by plots Production Production I II III Ave. kg/mu Compared to A A 9.8 9.9 10.0 9.9 368.1 0 B 13.1 13.5 12.9 13.2 460.8 +33.0% C 11.6 12.0 10.8 11.4 432.6 +15.8% D 10.9 11.2 11.4 11.2 381.4 +12.8% Treatments B is Significantly Greater than Treatment C & D and the Control (A) by Analysis of Variance at the 0.001 Level of Significance. Yields based upon the number of bulbs per plant and the average weight per bulb.

7

Fig.4. Chinese scientists measure individual plants to carefully calculate the +33% Total Production of plants within Treatment B, soil and foliar applied Agrostim. Fig.5. Comparative Onion bunch sizes on different fertilizer treatments.

Additionally, the Report indicates:

• Agrostim increased Chinese Cabbage yield by 280 to 410 kilograms per Mu.

• Improved plant growth & spread of roots

• Agrostim treated plants responded faster than control or plants receiving other Organic Fertilizer Treatments.

8

• Agrostim treated Celery & Green Onions were significantly heavier, taller plants

than the control plants or plants receiving other Organic Fertilizer Treatments.

• All Agrostim plants displayed a deeper, more vibrant green leaf color. Agrostim treated plants were more disease resistant.

• Based upon a production increases, at local prices the average farmer obtained

a net profit increase of 2.80 to 5.30X (280% - 530%) over the cost of the Agrostim.

China (PRC) - Mustard Green

Mustard Green - Brassica juncea Horticulture Branch, Agriculture Department Quang Dung Province

Agrostim studies were conducted in China using the following experimental design: Species: Brassica juncea Cultivar: Mustard Green Location: Quang Dung Province, Quang Zhow, Tin Hall District, China Dates: January 17 to March 17, 1997

9

Experimental Design: 3 Separate Treatments plus Control CK - Control, 60%of local customary fertilizer (urea)

A. CK + Agrostim 0.45 kilogram/acre soil applied (Dilution ratio 1:2,000)

B. CK + Agrostim 0.225 kilogram/acre soil & foliar applied (Dilution ratio 1:4,000)

C. CK + Agrostim 0.45 kilogram/acre foliar applied (Dilution ratio 1:566) Study Results: Table 1. Comparison of Production Rates, Gross Weight Increase, and Percentage of Agrostim Yield Increases over the Control (CK). Treatment Production by plots Production Production I II III Ave. per acre Compared to CK CK 46.65 47.20 47.80 47.22 1,967.5 0 A 47.65 48.75 49.80 48.73 2,026.7 +3.0% B 52.40 54.05 55.70 54.05 2,247.9 +14.3% C 54.70 54.65 55.20 54.85 2,281.2 +15.9% Treatments B & C are significantly greater than Treatment A and the Control (CK) by Analysis of Variance at the 0.001 Level of Significance.

Table 2. Growth Pattern and Plant Appearance Differentials Between Agrostim Treated and Control Grown Mustard Green. Treatment Ave. Plant Ave. Plant Chlorophyll Weight (Grams) Height (Centimeters) Levels CK 236 42.9 40.6 A 244 43.2 40.5 B 270 46.4 42.8 C 274 48.0 42.7 Treatments B & C are Significantly Greater than Treatment A and the Control (CK) by Analysis of Variance at the 0.001 Level of Significance.

10

Fig.1. Comparative Growth of Mustard Green. Soil applied Agrostim plants

(A) are on the left side, Control (CK) plants are on the right.

Fig.2. Comparative Growth of Mustard green. Soil and Foliar applied Agrostim

(B) plants are on the right, Control (CK) plants are on the left.

11

Fig.3. Comparative Growth of Mustard Green. Foliar applied Agrostim (C)

plants are on the right, Control (CK) plants are on the left.

Table 3. Growth Pattern and Plant Appearance Differentials Between Agrostim Treated and Control Grown Mustard Green Treatment Ave. Number Ave. Leaf Ave. Leaf of Leaves/Plant Length (Centimeters) Width (Centimeters) CK 10.1 38.6 19.8 A 10.2 39.0 20.4 B 10.8 41.6 21.6 C 10.9 42.4 21.8 Treatments B & C are Significantly Greater than Treatment A and the Control (CK) by Analysis of Variance at the 0.001 Level of Significance.

12

Fig.4. Comparative leaf growth for Agrostim treated plants (Left) versus the

Control (CK) plants (Right).

Additionally, the report clearly indicates Agrostim’s:

• Improved cooked leaf taste

• Improved plant growth & spread of roots

• Agrostim increased yield by an average of 16%

• Agrostim treated Mustard greens were significantly heavier, taller plants than the control plants

• Deeper more vibrant green leaf color

• Based upon a production increase of 313.7 Kung Gen/Acre, at local prices the average

farmer obtained a net profit increase of 3.30X (330%) over the cost of the Agrostim.

13

China (PRC) – Ornamental Flowers Petite dahlia, Creeping chrysanthemum, and Tiandong green leaf plant.

Agriculture Science Research Institute for Fengtai District, Beijing

To study the fertilizer effects of Agrostim™ organic based fertilizer on ornamental flowers, studies were initiated in August 1996 at the Agriculture Science Research Institute for Fengtai District of Beijing and the Jingfeng Seedling Plantation of Flower Town of Beijing, the Peoples Republic of China. These extensive tests were conducted with three species of flowers: Petite dahlia, Creeping chrysanthemum, and Tiandong green leaf plant.

The testing protocol consisted of Agrostim diluted at a ratio of 300:1 in water and foliar applied three times at weekly intervals versus the standard synthetic fertilizer regime of ammonium phosphate. Each treatment consisted of 50 individual plants in 20 cm clay flower pots with 4.5 kg of soil in each. The results of these intensive studies includes: A. Petite Dahlia: Results

1. Number of flowers/plant/pot Agrostim showed a significant increase over the standard regime at the 0.01% level of significance.

2. Number of buds/plant Agrostim gave an equal number of buds per pot.

14

Agrostim Standard Standard Agrostim Treatment Treatment Treatment Treatment B. Creeping Chrysanthemum

Agrostim treated chrysanthemum plants.

Results

1. Average plant height Agrostim showed a significant increase over the standard regime at the 0.01% level of significance.

2. Average flower diameter Agrostim showed a significant increase over the standard regime at the 0.05% level of significance.

15

3. Number of flowers in blossom Agrostim showed a significant increase over the standard

regime at the 0.05% level of significance.

C. Tiandong Green Leaf Results

1. Average plant height Agrostim showed a significant increase over the standard regime at the 0.01% level of significance.

Special Note: The test data was presented in a mathematical statistical fashion - as such, the plant growth data is expressed in terms of a “significance level” which is a statistical way to show the significant growth improvements seen for the Agrostim treated plants. The “level of significance” values given above, for example, at the 0.01% level says that the Agrostim treated plants would have higher growth and/or yields in 99 of 100 separate field tests. At the 0.05% level, Agrostim treated plants would have significantly higher growth and/or yields in 95 of 100 separate field tests. These levels of significance are indeed remarkably positive for the usage of Agrostim in the Peoples Republic of China. Summary and Conclusions: The plants tested were the principal flowers and plants on display for the October 1, 1996 - Chinese National Day. These plants were displayed in Tiananmen Square and on major streets and around government buildings in Beijing. These Agrostim efficacy tests were initiated to coincide with the October 1 date so the flowers would be colorful and in excellent condition for the Chinese National Day. Conclusions would include:

16

1) In comparison with the standard fertilizer regime, the “Agrostim” organic based fertilizer has obvious fertilization effects on the three subject plant species through 3 foliar applications of Agrostim.

2) In comparison with the standard fertilizer regime, the “Agrostim” organic

based fertilizer effects on Petite Dahlia reached outstanding levels of remarkableness.

3) In comparison with the conventional fertilization, the “Agrostim” organic based

fertilizer effects in terms of the number of flowers and the diameter of flowers of Creeping Chrysanthemum reached the outstanding level of remarkableness.

4) In comparison with the conventional fertilization, the “Agrostim” organic based

fertilizer effects in terms of the plant heights of the ornamental leaf plant Tiandong reached the outstanding level of remarkableness.

5) The “Agrostim” fertilizer is easy to apply, is non-poisonous and effectively odorless.

In can be used in big guest houses, hotels, and on large flower gardens and farms. 6) An active organization should be set up to popularize Agrostim’s application in

general “Flower Cultivation” in the Peoples Republic of China. China (PRC) – Water Spinach Water Spinach - Ipomoea aquatica

Si Ji Qing, West of Beijing Building on extensive vegetable studies of Agrostim™ in the United States, Vietnam, Egypt, Canada, Colombia, Syria, India, China, Honduras, and Guatemala, a series of Water Spinach - Agrostim™ studies were initiated at vegetable research facility at Si Ji Qing, West of Beijing. Studies were focused on Agrostim’s comparative impacts on increased plant growth (shoot, roots, leaves) and yield. STUDY DESIGN: Three total plots were established covering an area of 9 mu for the cultivation of common water spinach, Ipomoea aquatica. Agrostim was applied to 50% of the plants at a rate of 1 teaspoon of liquid Agrostim concentrate per 30 pounds of water for foliar application. The other 50% of the plants were fertilized with the normal fertilizer regime of green manure and 50 pounds of Urea per mu.

17

Fig.1. Water Spinach growth 14 days after fertilizer application. Agrostim treated plants are on the left side of the plate, normal fertilizer regime treated plants are on the right. RESULTS:

Fig. 2. Comparison of total Water Spinach growth at day 14. Agrostim treated

plants are on the left, urea/manure treated plants are on the right.

18

Table 1. Average growth parameters for Water Spinach at day 14. Sample sizes were greater than 400 individual plants per treatment. Agrostim Treatment Standard Treatment Plant height (cm) 14.5 12.0 Stem diameter (mm) 6.0 4.0 Leaf width (cm) 28.0 12.0 Root length (cm) 7.6 6.1

Fig. 3. Comparison of individual Water Spinach leaves at day 14. Agrostim

treated plants are on the left, urea/manure treated plants are on the right.

Fig. 4. Comparison of Water Spinach root growth at day 14. Agrostim treated

plants are on the left, urea/manure treated plants are on the right.

19

CONCLUSIONS:

1. Agrostim is an effective fertilizer for utilization on Water Spinach and related vegetable crops. Agrostim consistently gave overall yield increases of 30% to 40%.

2. Agrostim is a spray application fertilizer with foliar application an effective mode

of product delivery. Agrostim increased the nutritiously (iron) important leaf area by approximately 120% over the standard urea/manure regime.

3. Agrostim was found to be far more effective and less costly than Pen Shi Bao (a

Chinese foliar fertilizer). The average production cost calculated for Agrostim was approximately $1.13/mu (9.29 yuans/mu).

4. The positive controls, NP synthetic fertilizer(only) applied at a rate of 50

pounds/Mu may have provided a super nutrient rich media, especially considering the starting “manure rich” soils used. The ratio of synthetic fertilizer usage compared to Agrostim was approximately 48 to 1 (In terms of pounds applied per Mu). A cost benefit analysis may well establish Agrostim’s superior capabilities.

5. Agrostim treated water spinach plants showed significantly greater average plant

height (>0.01), greater average stem diameter (>0.01), grater average leaf width (>0.01), and greater average root length (>0.01) over the standard locally used urea/manure fertilizer via a “Paired T Statistic”.

6. Additional studies to “fine tune” the exact Agrostim requirements on a sustained

basis will clearly show the improved yields and increased disease resistance that is seen everywhere with Agrostim utilization.

China (PRC) – Rose Rose – Rosaceae Sp. Agricultural Sciences Research Institute for Flowers – Guang Dong Province

20

To study the fertilizer effects of Agrostim™ organic based fertilizer on ornamental flowers; studies were initiated in January 1997 at the Agriculture Science Research Institute for Flowers – Guang Dong Province, the Peoples Republic of China. These extensive tests were conducted with various species of Rosaeae to ascertain the optimal Agrostim dilutions for maximum plant growth and flower yields. Testing Protocol: The testing protocol consisted of several Agrostim dilutions of 200:1, 400:1, 600:1, 800:1, and 1,000:1 in water and foliar applied three times at weekly intervals versus the standard synthetic fertilizer regime of ammonium phosphate. Each treatment consisted of 25 individual plants in 20 cm clay flower pots with 4.5 kg of soil in each. Summary and Conclusions: The rose plants tested clearly showed a positive growth in the plants and the flowers at serial dilutions of Agrostim, even down to a very low concentration of 800:1 to 1000:1 dilutions. The standard recommended dilutions for Agrostim range between 300:1 and 400:1.

1. In comparison with the standard recommended fertilizer application rates, “Agrostim” organic based fertilizer benefits from a lower dilution applied via foliar application more frequently.

21

2. The results of this study further increase the “cost-benefit” ratios of Agrostim

utilization when compared to other products, especially the dangerous synthetic chemical fertilizers. Obtaining excellent study results at an 800:1 dilution will allow the Agrostim to cover more area, cutting the farmers fertilizer costs by as much as 50%.

3. In comparison with the conventional fertilization, the “Agrostim” organic based

fertilizer effects in terms of the plant heights of the ornamental leaf plant Tiandong reached the outstanding level of remarkableness.

4. The “Agrostim” fertilizer is easy to apply, is non-poisonous and effectively

odorless. In can be used in big guest houses, hotels, and on large flower gardens and farms.

5. An active organization should be set up to popularize Agrostim’s application in

general “Flower Cultivation” in the Peoples Republic of China. India – Sunflower Sunflower - Helianthus annus APC R&D Center, Mallapur, India

22

Sunflower has become widely cultivated in India as a source of low cost vegetable oil and protein. Sunflower is highly valued for its anti-cholesterol properties. Commercial scale cultivation of sunflower was initiated in 1972 throughout India, especially in Karnataka, Tamil nadu, Maharashtra and Andhra Pradesh. In Andhra Pradesh, principal cultivation areas include Kurnool, Chittoor, Ananthapur, Mehaboobnagar, Medak, Nalgonda, and Karimnagar. Generally, all sunflower cultivation is carried out via rainfed environmental conditions. With previous commercial scale and highly positive Agrostim Indian studies on tomatoes, sorghum, and roses, sunflower was chosen for study because of its commercial importance to the vegetable oil industry. Expanded Agrostim Sunflower studies were conducted in India using the following “experimental design”: Species: Helianthus annus Cultivar: Modern, National Seeds Corporation Location: APC R&D Center, Mallapur, India Dates: August-December 1996

23

R&D Site: The site was >4,300 ft2 of sandy loam soil with slight alkaline pH and good drainage. The water utilized in the study was highly saline with low sodium.

Experimental Design: 13 Separate Treatment Sets

1. Organic fertilizer1 - Farm manure (3,500 kg/ha) 2. NPK Fertilizer2 - Synthetic (80:50:30)

3. Agrostim3 - soil applied - rate 20 liters/ha/yr. 4. Agrostim3 - soil applied - rate 15 liters/ha/yr.

5. Agrostim3 - soil applied - rate 10 liters/ha/yr. 6. Agrostim4 - foliar applied - rate 20 liters/ha/yr. 7. Agrostim4 - foliar applied - rate 15 liters/ha/yr. 8. Agrostim4 - foliar applied - rate 10 liters/ha/yr.

9. Organic Fertilizer + Synthetic NPK5 (Current Standard) 10. Agrostim6 - soil + foliar applied - rate 20 liters/ha/yr. 11. Agrostim6 - soil + foliar applied - rate 15 liters/ha/yr. 12. Agrostim6 - soil + foliar applied - rate 10 liters/ha/yr. 13. Negative Control (No fertilizers)

Application Notes: 1Farm manure and neem cake powder were applied on days 30, 45, & 60.

2NPK was applied on days 30 & 45 as urea, super phosphate, and murate of potash. 3Agrostim soil application at days 15, 30, & 45. 4Agrostim foliar application at days 30, 45, & 60. 5Neem cake and NPK as above in items 1 & 2, at days 30 & 45. 6Agrostim soil & foliar application at days 15, 30, 45, & 60.

Results: Table I. Average Plant Height (Centimeters), Average Flower Head Diameter, and Average Total Leaf Area in Square Centimeters for Agrostim expanded field studies. In all cases, Agrostim foliar and/or soil applied organic based fertilizer was “significantly greater and better” than the standard Indian regimes of Organic Manures and/or Synthetic Chemical Fertilizers at the P>0.01 levels (ANOVA).

Treatment Set Plant Height Flower Head Leaf Area

(CM) Diameter (CM) (CM2) 1. Organic fertilizer 56.6 10.6 92.81 2. NPK Fertilizer 58.6 11.5 178.90 3. Agrostim-soil 20 l 57.6 13.7 145.07 4. Agrostim-soil 15 l 58.2 15.0 195.64 5. Agrostim-soil 10 l 62.2 15.2 231.85 6. Agrostim-foliar 20 l 75.8 18.5 373.24 7. Agrostim-foliar 15 l 78.6 19.5 336.91 8. Agrostim-foliar 10 l 66.0 14.9 299.99 9. Organic+Synthetic 68.0 14.4 218.21 10. Agrostim-soil+foliar 20 l 55.4 14.3 266.96 11. Agrostim-soil+foliar 15 l 50.8 13.8 145.44 12. Agrostim-soil+foliar 10 l 46.6 11.0 99.71 13. No Fertilizer 46.0 7.2 98.34

24

Table II. Average Number of Flowers Harvested, Average Yield of Sunflower Seeds in Kilograms/Hectare, and Average Number of Sunflower Seeds per Flower for Agrostim expanded field studies. In all cases, Agrostim foliar and/or soil applied organic based fertilizer was “significantly greater and better” than the standard Indian regimes of Organic Manures and/or Synthetic Chemical Fertilizers at the P>0.01 levels (ANOVA). Treatment Set No. Flower Yield No. Seeds

Harvested (KGS/HA) Per Flower 1. Organic fertilizer 47 209.25 449 2. NPK Fertilizer 27 220.37 541 3. Agrostim-soil 20 l 48 588.89 399 4. Agrostim-soil 15 l 40 675.93 406 5. Agrostim-soil 10 l 37 434.26 390 6. Agrostim-foliar 20 l. 43 663.89 880 7. Agrostim-foliar 15 l 50 807.41 600 8. Agrostim-foliar 10 l 29 406.48 718 9. Organic+Synthetic 38 632.41 521 10.Agrostim-soil+foliar 20 l 44 451.85 362 11.Agrostim-soil+foliar 15 l 37 510.19 556 12.Agrostim-soil+foliar 10 l 39 321.30 140 13.No Fertilizer 22 135.19 264 Table III. Average Seedling Number at 10 days, Average Oil Content (%), and Average Sunflower Seed Weight per 1,000 Seeds for Agrostim expanded field studies. In all cases, Agrostim foliar and/or soil applied organic based fertilizer was “significantly greater and better” than the standard Indian regimes of Organic Manures and/or Synthetic Chemical Fertilizers at the P>0.01 levels (ANOVA). Treatment Set No. Seedlings Oil Content Seed Weight

10 Days (%) Per 1,000 (in gms) 1. Organic fertilizer 59 35.35 54 2. NPK Fertilizer 45 34.44 49 3. Agrostim-soil 20 l 76 34.95 58 4. Agrostim-soil 15 l 63 35.59 60 5. Agrostim-soil 10 l 57 37.47 53 6. Agrostim-foliar 20 l. 60 40.68 56 7. Agrostim-foliar 15 l 70 37.88 67 8. Agrostim-foliar 10 l 54 41.25 54 9. Organic+Synthetic 51 38.37 53 10. Agrostim-soil+foliar 20 l 62 37.45 50 11. Agrostim-soil+foliar 15 l 55 39.65 45 12. Agrostim-soil+foliar 10 l 59 34.15 52 13. No Fertilizer 43 37.03 47

25

Fig. 1. Sunflower Growth at day 15. Standard Indian fertilizer regime in foreground

with Agrostim foliar applications in the background.

Fig.2. Sunflower grown using Indian standard Organic fertilizer manures at day 45. Flower size can be judged by technicians arm in picture.

26

Fig.3. Sunflower grown using Agrostim foliar application at day 45.

Flower size can be judged by technicians arm in picture.

Fig.4. Sunflower grown using Agrostim soil application at day 45.

Flower size can be judged by technicians arm in picture.

27

Fig. 5. General Treatment Sample Size for the Agrostim expanded Sunflower

field study. In summary, the report clearly indicates that Agrostim:

• Increased yield overall by 27.67% • Improved growth & spread of roots • Increased oil content of seeds by 16.51% • Provided excellent response in Saline soil & water • Eliminated “flower drop” • Improved seed filling of flower heads • Significantly reduced of any phytotoxic effects

China (PRC) – Tomato Tomato - Lycopersicon esculentum Flower Town of Fengtai District, Beijing

28

Building on extensive Tomato studies of Agrostim™ in the United States, Vietnam, India, China, Honduras, and Guatemala, a series of Agrostim™ studies were initiated at several greenhouse facilities in the Fengtai District of China (Yushu Village & Middle Shed of Xinfadi). Studies were focused on Agrostim’s comparative impacts on increased plant growth and yield, increased disease resistance, improved fruit palatability and increased shelf life. Middle Shed of Xinfadi: Agrostim™ applications increased tomato yield over the control plants (76.7 to 34.6 jin) which approximates a 42% yield increase. This translates into a yield improvement of 2,070 Jin/Mu (54.5%).

29

Fig. 1. Agrostim tomato yield at Middle Shed of Xinfadi, China. Yushu Village: Agrostim™ applications increased tomato yield over the control plants (123.6 to 116.6 jin) which approximates a 6% yield increase. This translates into a yield improvement of 305 Jin/Mu (6%).

Fig.2. Agrostim usage - Yushu Village, China. CONCLUSIONS:

1. Agrostim™ is an effective fertilizer for utilization on vegetable crops.

30

2. Agrostim™ is a spray application fertilizer with foliar application an effective mode of product delivery.

3. The soils used in the studies was relatively “good” in quality with a relatively high organic content and contained NPK. The soil pH in both studies was above 7.0 which may require a little more Agrostim™ to properly acidify the soil and stabilizing the micro-ecosystem.

4. The positive controls, NP synthetic fertilizer (only) applied at a rate of 77 pounds/Mu may have provided a super nutrient rich media, especially considering the starting “rich” soils used.

5. The ratio of synthetic fertilizer usage compared to Agrostim™ was approximately 77 to 1 (In terms of pounds applied per Mu). A cost benefit analysis may well establish Agrostim’s superior capabilities.

6. Additional studies to “fine tune” the exact Agrostim™ requirements on a sustained basis will clearly show the improved yields and increased disease resistance that is seen everywhere with Agrostim™ utilization.

India – Tomato Tomato - Lycopersicon esculentum Shameerpet, Hyderabad

Agrostim studies were conducted in India using the following experimental design: Species: Lycopersicon esculentum Cultivar: Pusa Hybrid-1 Location: Shameerpet, Hyderabad, India Dates: May-September 1996 Experimental Design: 6 Separate Treatment Sets 1. Agrostim - foliar applied - rate 10 liters/hectare 2. Agrostim - soil applied - rate 10 liters/hectare 3. Agrostim - soil + foliar applied - rate 10 liters/hectare 4. Agrostim - seed treated - 250 ppm 5. Inorganic fertilizer - NPK (100 kg; 80 kg; 80 kg/hectare) 6. Organic fertilizer - Farm manure (3,500 kg/hectare) Results: Yield: Agrostim¹ (foliar) = 100% Inorganic fertilizer = 31% Organic fertilizer = 40%

¹Total Marketable Fruit Yield, plus highest number of fruit/plant, largest weight/fruit/plant

31

Fleshiness: Agrostim = Good Inorganic fertilizer = Poor Organic fertilizer = Poor Size & Shape Agrostim = Good of Fruit: Inorganic fertilizer = Poor Organic fertilizer = Poor

Disease Agrostim (all groups) = No disease Resistance: Inorganic fertilizer = Leaf spot & fruit rot

Organic fertilizer = Severe fruit rot

Shelf Life: Agrostim (all groups) = 21 days Inorganic fertilizer = 14 days Organic fertilizer = 14 days

Fig. 1. Agrostim Treated Seedling 2 days after transplanting

32

Fig. 2. Untreated Seedling 2 days after transplanting transplanting

Fig. 3. Tomato’s intercultivated with Kurpi. Shameerpet, Hyderabad, India.

33

Fig.4. Foliar Applied Agrostim Yields - 50 days after transplantation.

Fig. 5. Standard Synthetic Fertilizer Yields - 50 days after Transplantation.

34

The report clearly indicates that Agrostim:

• Improved fruit taste • Improved growth & spread of roots • Decreased economic loss from fruit spoilage in transport • Reduced internodal lengths • Deeper more vibrant green leaf color • Elimination of “flower drop”

India – Grain and Fodder

Sorghum bicolor The National Research Center for Sorghum, and The All India Sorghum Improvement Project Sorghum is a vigorous annual grass adapted to the drier environmental zones in Africa, Asia, the Americas, and Australia. Annual world production approaches

40-45 million tons from approximately 40 million hectares of land, making sorghum the 5th most important cereal. Sorghum is a vital source of grain and fodder for many of the poorest countries of the world, especially those in the arid tropics. Sorghum is adapted to dry poor soil ecologies through an extensive root system and waxy bloom on leaves to reduce water loss.

35

Two large scale independent controlled field studies have been completed in India to investigate potential grain and fodder yield increases at different methods and rates of Agrostim application over the growing season in Tropical Arid environmental conditions. A. Sorghum Grown under Rabi Conditions: Institute: Indian National Research Center for Sorghum Rajendranagar, India Season: Rabi – October to November 1996 Sorghum Type: Genotype M 35-1 Soil Type: Medium deep black soil with an elevated soil pH. Table 1. Effect of application of Agrostim on Sorghum grain and fodder yields. Treatments Grain Yield Fodder Yield Agrostim & Control kg/ha kg/ha _______________________________________________________________ In Soil @ 15 l/ha 2496 5056 In Soil @ 20 l/ha 3041 4278 Soil + Foliar @ 15 l/ha 2889 4978 Soil + Foliar @ 20 l/ha 3144 4200 Foliar Only @ 15 l/ha 2656 5133 Foliar Only @ 20 l/ha 2911 5288 Control (Normal NPK) 1625 3440 ________________________________________________________________ B. Sorghum Grown under Kharif Conditions: Institute: All India Coordinated Sorghum Improvement Project Sorghum Research Station, Palbhani, India Season: Kharif – 1996/1997 Sorghum Type: Genotype CSH - 9 Soil Type: Medium deep black soil with high initial nutrient status. Table 2. Effect of application of Agrostim on Sorghum grain and fodder yields. Treatments Grain Yield Fodder Yield Agrostim & Control kg/ha kg/ha ________________________________________________________________ In Soil @ 15 l/ha 3156 7593

36

In Soil @ 20 l/ha 3717 7778 Soil + Foliar @ 15 l/ha 3565 8890 Soil + Foliar @ 20 l/ha 4000 9260 Foliar Only @ 15 l/ha 3463 7593 Foliar Only @ 20 l/ha 3574 7963 Control (Normal NPK) 2722 6852 __________________________________________________________________ India - Tea

Increased Growth & Yield on Tea and Rice E. I. D. Parry (India) Limited - Agro Industries, Madras, India Increased plant quality and vigor obtained from Agrostim utilization greatly enhances the Yield of Tea Leaves but, as well, enables the plants to resist the attack of Pathogenic Plant Diseases (Fungi, Bacteria, & Viruses), Nematodes and Insects. Large scale field studies in India compared: a) Agrostim organic based fertilizer as 1% solutions applied foliar two (2) times to b) Standard NPK synthetic chemical fertilizers.

37

Each study consisted of 500 mature tea plants which are harvested biweekly (26 times/year) After one month the Agrostim treated tea plants yielded an average of 104 kilograms of prime green leaf more per hectare than the Standard fertilizer regime. This ultimately yields 23 kilograms of finished dried black tea. This calculates to an approximately 22% increase in black tea yield by AGROSTIM treated plants versus the Standard regime of synthetic NPK fertilizers. Large scale Agrostim utilization will be initiated with the April initiation of the primary growing season for tea after the first rains. Plantation managers estimate greater yield improvements during this tea growing period. Increased plant quality and vigor obtained from Agrostim utilization greatly enhances the yield of rice: Season: Rabi – December 1996- April 1997 Rice Type: Tellahamsa

Design: 3 x foliar spray AGROSTIM at 15 l/ha dosage; Control (60:40) kgs Urea and Phorate standard regime.

Results: Parameters Agrostim Control Improvement ________________________________________________________________ Ave Plant Height 110 cm 100 cm +10% Ave No. tillers/hill 20 15 +25% Ave No. panicles/hill 18 9 +50% Ave No. grains/panicle 127 85 +50% Ave No. grains/hill 2,286 765 +300% Ave Test Weight 22.28 17.72 +25% Ave Yield kg/ha 3,774.18 2,669.58 +40% ___________________________________________________________________

38

Vietnam – Silk Plant Yield Increases and Improvement of Silk White Mulberry - (Dâu Tam) The Baoloc School of White Mulberry Baoloc, Lamdong Province

Large scale independent controlled field studies have been completed in Vietnam to investigate potential improvement of the yield and overall “quality” of White Mulberry leaves for consumption by silkworm caterpillars. Besides the excellent plant increases in leaf material biomass, the researchers found that silkworm caterpillars feeding on Agrostim treated leaves had a significant decrease in caterpillar mortality (from 18% to 2%), a significant

39

shorting of the larval period by 25%, larvae and cocoons larger by an average of 30%, an increase in silk yield per caterpillar by approximately 30%, and a silk product that was of higher quality in terms of taking and holding color dyes. Table 1. Effect of application of Agrostim on White Mulberry one month growth & yields. Treatments Plant Height Plant Diameter No. Leaves/Stem ________________________________________________________________ Soil Only @ 20 l/ha 59 cm (+16%) 5.4 mm (+17%) 15 (+25%) Soil + Foliar @ 20 l/ha 63 cm (+22%) 6.0 mm (+30%) 17 (+42%) Foliar Only @ 20 l/ha 54 cm (+7%) 5.0 mm (+9%) 13 (+8%) Control (Normal NPK) 51 cm 4.6 mm 12