ihi lab muhas presentation july 15

1

INTRODUCTION

TO IHI LABORATORIES

Ramadhani Ngwale Musa Maganga

Christopher Membi Moses Gwamaka

2

GOAL

Provide information on what is going on in IHI

Laboratories

3

Overview

Ifakara Health Institute (lHI)

Non-profit research and training institute whose

mission is to develop and sustain a district-based

health research and resource centre capable of

generating new knowledge and relevant

information for public health policy and actions

IHI is among the leading health research institutes

in Africa

4

Overview

LABORATORY UNIT

Core service unit of IHI, was established to support laboratory based research activities

Two modern laboratories, located in

Ifakara and

Bagamoyo

IHI laboratory unit - have optimal resources including man power and infrastructures (buildings and equipments)

5

IHI LAB ACTIVITIES

RESEARCH:

Provide laboratory-based technical support to the research

projects

- Currently the 2 labs supports over 16 projects

TRAINING:

Provide training and supervision to local and international

students during their field practicals

Provide practical training and supervision to laboratory

technicians/ visiting scientists

In future – part of PhD, MSc students traininf – Nelson

Mandela –Arusha Institute of Science and Technology

6

SERVICES:

Provide quality and timely diagnostic services to study patients

and non study patients

Analyze samples from other Institutions

- NIMR-Tanga,

- KCMC,

- Tanzania Tsetsefly Research Institute and

- Zanzibar Malaria Control Programme (ZMCP)

IHI LAB ACTIVITIES

7

OPERATIONAL LAYOUT

Head of Laboratory services

Laboratory manager

QA Manager

Director

Section leader

INSTITUTE

Branch/ laboratory

Section/functional unit

8

SECTION 1. Biochemistry

ACTIVITIES Electrolyte analysis using Cobas integra – 60 samples /cycle

2. Hematology Full blood picture- Sysmex

3. Immunology ELISA,PBMC Isolation and stimulation, IPT 10 ELISA, CD4/CD8 Count

4. Molecular biology Genotyping of pathogens – TB and malaria by conversional PCR, RT-PCR and Massarray technology

5. Microbiology 6. Parasitology 7. Helminthology 8. BSL 2 Facility + TB 9. BSL 3 (Virology)

Analysis of blood, urine, stool, CSF samples Detection of malaria parasites Diagnosis of helminthes For isolation and culturing Mycobacteria Under construction

Operational layout – BAGAMOYO LAB

9

SECTION 1. Biochemistry

ACTIVITIES Biochemical tests using automated equipments

2. Hematology ESR, full blood picture- Sysmex

3. Immunology ELISA,P24, Rapid Plasma Regin, CD4/CD8 Count

4. Molecular biology Mosquito genotyping, Viral load determination, DNA/RNA extraction, - In the near future - SEQUENCING

5. Microbiology 6. Parasitology 7. Histopathology

Analysis of blood, urine, stool, CSF samples Detection of malaria parasites , Diagnosis of helminthes Diagnostic purposes- tumors

Operational layout – IFAKARA LAB

10

LAB EQUIPMENTS

The IHI laboratories are equiped with basic and

advanced equipments that allows conventional

and state-of-the art analyses

MOLECULAR BIOLOGY SECTION

Conventional PCR - Genotyping – malaria vectors

GEN PROBE system

A system of nucleic acids amplification – uses rRNA as template

- 10X more sensitive than conventional PCR

- Currently we use for diagnosis of STDs-

LAB EQUIPMENTS - MOLECULAR BIOLOGY SECTION

Diagnosis of infectious diseases

Detection of genetic diseases

Determination and quantitation of

microbial contaminants in samples

Gene expression studies

Real time PCR-Machine (Mx 3005, StrataGene)


Near Infrared spectroscopy

For mosquito genotyping and age determination -under validation

Originally – used in Agricultural entomology


15

MassArray for Genotyping, Comparative Sequencing

& Quantitative Gene Analysis

TB drug resistance study in Bagamoyo


16

HISTOPATHOLOGY For disease diagnosis – recently established in Ifakara, Tissue preparation will be done in Ifakara, the diagnosis will be done by pathologist in Switzerland instantly (Telepathology)

17

IMMUNOLOGY

FLOW CYTOMETER (FCM)

FCM – for phenotypic and functional analyses of immune cells

Currently - is the only technology platform that suitably can analyze the complex components of the immune system

FACSCALIBUR (3 or 4 color) 8 color flow cytometer

18

BACTEC for blood culture

BACTERIOLOGY

19

Electrolyte analysis using (1) Cobas integra and (2) Vitrous DT

BIOCHEMISTRY

20

Temperature controlled Storage facillities

SAMPLE STORAGE

21

HUMAN CAPACITY

A LABORATORY is defined by the PEOPLE who work in it

22

HUMAN CAPACITY

LAB STAFF

IFAKARA LABORATORY

Laboratory scientists 12

PhD 2

MSc. 4

BSc. 6

Laboratory Technicians 8

Adv Diploma 1

Diploma 2

Certificate 5

Lab attendants 2

BAGAMOYO LAB

Laboratory scientists 13

PhD 1

MSc. 2

BSc. 10

Laboratory Technicians 18

Adv Diploma 2

Diploma 2

Certificate 11

Lab attendants 3

23

STAFFING THE LAB

Are recruited by Research Projects depending on

- What type of expertise is required for the project

- Funding level – how much grant money the project have

- How much time will be devoted for training (Experienced

intermediate or little experience)

Current lab staff education backgrounds:

SUA – BSc (Biotechnology and Laboratory science)

UDSM – BSc (Biotechnology and Molecular Biology)

Tumaini – BSc (Medical Laboratory)

RUCO - BSc (Medical Laboratory)

MUHAS – Advanced diploma in Medical Laboratory

Certificates in medical laboratory (Singida, Mvumi, Ikonda,

RUCO)

QUALITY ASSESMENT (QA)

IHI laboratory works according to Good Clinical

Laboratory Practice, including using SOPs for sample

handling, testing, reporting and storage

24


IHI laboratories equipment and procedures are subject to both

internal and external quality control (QC)

Internal QC is performed daily on all analyzers and all records are

stored within the laboratory for verification

IHI internal quality assurance personnel perform the general

assessment and auditing of the laboratory activities

Also

IHI performs external QC for malaria slide reading with KEMRI

Perform QA for malaria RDTs from ZMCP

25


External QC is performed in collaboration with several external

agencies

Microscopy - National Institute for Communicable Diseases (NICD), SA.

Hematology - Sample surveys with Birmingham Quality (Birmingham, UK).

Biochemistry - Sample surveys with UK NEQAS, (Sheffield, UK).

IHI laboratory activities (Bagamoyo) are currently subjected to external

quality assessment through Contract Laboratory Service (CLS) and

the GlaxoSmithKline quality assurance manager, SA.

26

27

CHALLENGES

Research environment is challenging requires flexibility - thinking,

working hours, optimization of protocols (which works better)

Technological challenges - technology is changing very fast –

Automated, high throughput equipments are being developed, -

therefore on job training is necessary

Some research materials are not available in the local market

therefore require importation from abroad – Takes long time of

protocol optimization – lack of flexibility and options

28

THE END

AHSANTENI SANA

ihi lab muhas presentation july 15

Documents