linked in march24v6
TRANSCRIPT
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Engineer “Arg-Gly-Asp” (RGD) epitopes for multi-valent display
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RGD: stem-cell
adhesion epitopealso target cancer
cells that up-regulate integrin
TYMV with RGD substitution at residue 44
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Wild-type 70°C
N-term mutant 70°C
pH-dependent enhanced stabilityMutate internal scaffold N-terminus of TYMV CP
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Robust TYMV virus-like particles in E. coli
In vitro reassembly from a denatured
state
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After many monthscrystal structure
data for TYMV and mutants!
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TYMV can form a molecular “container” after release of RNA, nano-inspired applications with a protein shell with a small “hole”
After freeze-thaw
before
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TYMV dual-subgenomic RNA for expression of GFP and GUS. Future downstream use for vaccine development
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Biophysical studies of TYMVCharacterization empty, virion and intermediate particles
Northerns, Westerns, gradient centrifugation, etc.
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Previous Work (M.S degree at OHSU): Signaling of a Cytomegalovirus chemokine receptor US28: plenty of cloning, tissue culture, immuno-precipitation/fluorescence and construction of CMV knockout mutants
US28chemokine
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Recent TYMV article & (cover image) Jan 2011,more publications to come