mikro isolasi bakteri
TRANSCRIPT
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PRAKTIKUM MIKROBIOLOGI DAN PARASITOLOGI 21
ISOLASI MIKROORGANISME
LAPORAN PRAKTIKUM MIKROBIOLOGI DAN PARASITOLOGI
PERCOBAAN IV
ISOLASI MIKROORGANISME
OLEH :
NAMA : KHADIJAH PURNAMASARI
NIM : O1A114141
KELAS : D 2014
KELOMPOK : IV
ASISTEN : AYU RUBAK RERUNG
LABORATORIUM FARMASI
JURUSAN FARMASI
FAKULTAS FARMASI
UNIVERSITAS HALU OLEO
KENDARI
2016
KHADIJAH PURNAMASARI AYU RUBAK RERUNG
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ISOLASI MIKROORGANISME
KATA PENGANTAR
Puj !"u#u$ !%"% &%'j%(#%' #)*%+$%( A,,%* S-T "%'.
(),%* /)/)$#%' $%*/%( !)$(% #%$u'%N"% #)&%+% !%"%
!)*'..% )$*%!, /)'"),)!%#%' ,%&$%' &$%#(#u/
/#$,. +%' &%$%!(,. ' "%'. %,*%/+u,,,%*
()&%( &%+% 3%#(u'"%
L%&$%' /#$,. +%' &%$%!(,. /)'.)'%
!,%! /#$$.%'!/) ' +!u!u' +)'.%' /%#!u+ +%'
(uju%' u'(u# /)/%'(u /%*%!!3% /%u&u' /%*%!!3 +%,%/
/)/&),%j%$ ()'(%'. /#$,. +%' &%$%!(,. S),%'
(u +*%$%%' +%&%( /)/)$#%' ,/u &)'.)(%*u%' /)'.)'%
!,%! /#$$.%'!/) +%,%/ /#$,. +%'
&%$%!(,.
S%"% /)'"%+%$ %*3% ,%&$%' ' /%!* j%u* +%$
!)/&u$'%5 ,)* #%$)'% (u #$(# +%' !%$%' +%$ !)/u%
&*%# "%'. )$!%( /)/%'.u' !),%,u #%/ *%$%%' +)/
#)!)/&u$'%%' ,%&$%' '
K)'+%$5 07 A&$, 2016
P)'u,!
DAFTAR ISI
KHADIJAH PURNAMASARI AYU RUBAK RERUNG
O1A114141
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PRAKTIKUM MIKROBIOLOGI DAN PARASITOLOGI 21
ISOLASI MIKROORGANISME
KATA PENGANTAR88888888888888888888888888888888888888888
DAFTAR ISI888888888888888888888888888888888888888888888
BAB I PENDAHULUAN
A LATAR BELAKANG888888888888888888888888888888888881
B RUMUSAN MASALAH88888888888888888888888888888888882
C TUJUAN PRAKTIKUM8888888888888888888888888888888882
D MANFAAT PRAKTIKUM88888888888888888888888888888882
BAB II TINJAUAN PUSTAKA
A DASAR TEORI888888888888888888888888888888888888889
B URAIAN BAHAN88888888888888888888888888888888888886
BAB III METODE PRAKTIKUM
A -AKTU DAN TEMPAT PRAKTIKUM888888888888888888888887
B ALAT DAN BAHAN888888888888888888888888888888888887
C PROSEDUR KERJA8888888888888888888888888888888888811
BAB IV HASIL DAN PEMBAHASAN
A HASIL PENGAMATAN888888888888888888888888888888888814
B PEMBAHASAN88888888888888888888888888888888888888881
BAB V PENUTUP
A KESIMPULAN88888888888888888888888888888888888888821
B SARAN88888888888888888888888888888888888888888888821
DAFTAR PUSTAKA88888888888888888888888888888888888888822LAMPIRAN888888888888888888888888888888888888888888888888
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ISOLASI MIKROORGANISME
BAB I
PENDAHULUAN
A. LATAR BELAKANG
M#$,. +/u,% !)j%# +()/u#%''"%
/#$!#& +%' /)'j%+ +%'. "%'. !%'.%( &)'('.
+%,%/ ,. !)(),%* Lu! P%!()u$ +%&%(
/)'j),%!#%' &$!)! )$/)'(%! %'..u$ ;3')< +%'
/)/u%( =%#!' $%)! P)$#)/%'.%' ,. "%'.
&)!%( &%+% %%+ #)17 P%+% (%*u' 1740 /#$!#&
),)#($' +#)/%'.#%' +%' +!)/&u$'%#%' P%+% +)#%+)
()$!)u( /)(+) u++%"% =$u! ju.% +&)$#)'%,#%'
+%' &)'.)(%*u%' ()'(%'. =$u! )$#)/%'. +)'.%'
>)&%( M#$,. /+)$' /)'j%'.#%u %'"%# +%'.
u!%*% /%'u!% ()$/%!u# &)'.)/%'.%' &$+u# %$/%!5
&)'..u'%%' /)(+) >'($, #u%,(%! +%,%/ &$+u#!
&%'.%' +%' &$+u# !u!u5 &)'.)'+%,%' /#$$.%'!/)
&)'")% &)'"%#( +&)$%$%' *%! +%' %&,#%!
'+u!($" /#$$.%'!/)
M#$,. /)$u&%#%' >%%'. +%$ ,/u ,.
"%'. #*u!u! /)/&),%j%$ j%!%+j%!%+ $)'#
M#$,. )$%!%, +%$ %*%!% "u'%' "%(u />$!
"%'. )$%$( #)>,5 ! "%'. )$%$( *+u& +%'
,.! "%'. )$%$( &)'.)(%*u%' S)*'..% !)>%$%
!'.#%( +%&%( +%$(#%' %*3% /#$,. %+%,%*
,/u "%'. /)/&),%j%$ ()'(%'. /%#*,u#/%#*,u# "%'.
#)>,#)>, M%#*,u#/%#*,u# "%'. #)>,#)>,
()$!)u( +!)u( ju.% +)'.%' /#$$.%'!/)5
/#$%5 /#$% %(%u j%!%+ $)'#
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ISOLASI MIKROORGANISME
I!,%! /)$u&%#%' /)(+) "%'. +.u'%#%' u'(u#
/)/!%*#%' %(%u /)/'+%*#%' /#$% ()$()'(u +%$
!u%(u ,'.#u'.%'5 !)*'..% +&)$,)* #u,(u$ /u$'
%(%u %##%' /u$' Ku,(u$ /u$' %,%* #u,(u$ "%'.
!),!), /#$%'"% )$%!%, +%$ &)/),%*%' +%$ !%(u
!), (u'..%, B))$%&% >%$% "%'. +,%#u#%' u'(u#
/)'.!,%! /#$$%.%'!/) %'(%$% >%$% .$)!%'
;!treak plate%$% (%u$%'?(u%'. ; pour plate%$% !)%$ ;spread plate%$% &)'.)'>)$%'
;dilution plate< !)$(% />$/%'&u,%($ B)$+%!%$#%'
u$%%' +%(%!5 /%#% +,%#u#%',%* &$%#(#u/ /)'.)'%
I!,%! M#$% '
B. RUMUSAN MASALAH
Ru/u!%' /%!%,%* +%,%/ &$%#(#u/ ' %+%,%*
%.%/%'%#%* >%$% /)/!%*#%' /#$% +%$ >%/&u$%''"%
!)*'..% ++%&%( #u,(u$ /u$'@
C. TUJUAN PRAKTIKUM
Tuju%' +%$ &$%#(#u/ ' %+%,%* u'(u# /)/!%*#%'
/#$% +%$ >%/&u$%''"% !)*'..% ++%&%( #u,(u$ /u$'
D. MANFAAT PRAKTIKUM
M%'%%( +%$ &$%#(#u/ ' %.%$ /%*%!!3%
/)'.)(%*u >%$% /)/!%*#%' /#$% +%$ >%/&u$%''"%
!)*'..% ++%&%( #u,(u$ /u$'
.
BAB II
TINJAUAN PUSTAKA
KHADIJAH PURNAMASARI AYU RUBAK RERUNG
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ISOLASI MIKROORGANISME
A. DASAR TEORI
M#$,. %+%,%* ,/u ()'(%'. #)*+u&%'
/#$$.%'!/) %'(%$% ,%' /$,.5 !,.5
$)&$+u#! +%' &)'")%$%' /#$$.%'!/) Ru%'.
,'.#u& /#$,. %'(%$% ,%' %+%,%* ()$
.)')!!5 &)'")%$%' /#$$.%'!/) !)>%$%
#!/&,(%'5 /)+% #u,(u$ u'(u# #u,(=%!
/#$$.%'!/)5 /)(+) !,%! +%' +)'(#%!
/#$$.%'!/)5 &)$%'%' /#$$.%'!/)5 &)'.)'+%,%'
/#$$.%'!/)5 &%(*.)')!! /#$$.%'!/) +%'
$)#%"%!% .)')(# ;H%$(5 AS5 201, %(%u $)'# %,%*
/#$$.%'!/)5 /#$'% %!%, #%(% />$! #)>, +%'
! *+u& J%!%+j%!%+ $)'# "%'. +)/#%' #)>,'"%
(+%# +%&%( +,*%( +)'.%' /%(% D%&%( +,*%(
+)'.%' /)'..u'%#%' %,%( u'(u# /)/&)$)!%$ )'+% "%'.
(+%# +%&%( +,*%( /%(% A,%( ()$!)u( +#)'%,
+)'.%' '%/% /#$!#& ; A+%/5S51772
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PRAKTIKUM MIKROBIOLOGI DAN PARASITOLOGI 21
ISOLASI MIKROORGANISME
M#$% "%'. +%&%( +/%'%%(#%' !)%.%
)$(,)$ +%'(%$%'"% %+%,%* /#$% &)'%/%(
*%$%5 &)'.#%( *%$%5 +%' &)/%'(%& %.$).$%(
S)>%$% %,%/5 /#$% + %,%/ +()/u#%' +%,%/
&&u,%! >%/&u$%' U'(u# /)/&)$,)* %#%' /u$'
+%&%( +,%#u#%' !,%! "%'. +%3%, +)'.%'
&)'.)'>)$%' )$('.#%( P$!)! !,%! /#$%
%+%,%* /)/!%*#%' /#$% !%(u +)'.%'
/#$% ,%' "%'. )$%!%, +%$ >%/&u$%' )$%.%
/#$% u'(u# +%&%( /)/&),%j%$ !%( %#%'5
/$,. +%' !%( /#$% ,%''"% ;Pu!&(%!%$
+##5 2012%$% /%!!%, +%' (u$u''"% #u%,(%! &%!#%
&%')' Pseudomonas +%' Aeromonas +%&%( /)'")%#%'
#)/%(%' ,%$=% $%ju'.%' ;Portunus pelagicus) /%u&u'
"%'. ,%''"% "%(u Callinectes sapidus5 '%/u' *%,
' +%&%( +*'+%$ +)'.%' /)(+) %$ /)'.%,$ +%'
&)/)$%' +)!')#(%' ;Su(#'3%(5 2019)&%(%'
()$>%&%'"% #)!)(/%'.%' $)%#!5 !)*'..% /)'%##%'
!u*u '#u%! +%&%( /)/&)$>)&%( ()$>%&%'"%
#)!)(/%'.%' $)%#!5 '%/u' &%+% !u*u ('.. ,)*
+/'%' ()$j%+ +!!!%! +%$&%+% %!!!%!5
KHADIJAH PURNAMASARI AYU RUBAK RERUNG
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ISOLASI MIKROORGANISME
!)*'..% &)$!)' B?T /)'j%+ ,)* $)'+%* +%' +%&%(
/)'u$u'#%' ,/( +)()#! +%' #)(),(%' Su*u
'#u%! %'"%# +,%#u#%' &%+% 4C5 2C %(%u 9C5
&)$,u +()'(u#%' &u,% 3%#(u '#u%! &%+% !u*u
()$!)u( ;Su!, +##5 200
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P)/)$%' :(+%# )$%u %(%u %u ,)/%*5
)$%!% /u!,%. &%+% ,+%*
K),%$u(%' :(+%# ,%$u( +%,%/ %$ +'.'5
+%' ,%$u( +%,%/ %$ /)'++*
K).u'%%' :!)%.% %*%' &)/%+%( /)+u/
P)'"/&%'%' :+%,%/ 3%+%* ()$(u(u& %#
2 Au%+)!( ;Ditjen POM E+! IV5 177 : H%, 112%/&u$ +)'.%' %$ +%'
&$%#(! )$>%/&u$ +)'.%' !)/u%
&),%$u( $.%'#
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ISOLASI MIKROORGANISME
K).u'%%' :A'( /#$%5 +)!')#(%'5
&),%$u(5 &)')($%! #u,(
P)'"/&%'%' :-%+%* ()$(u(u& $%&%( j%u* +%$
%&
4 Beef Extract ;D(j)' POM E+! IV5 177 : H%,
112(
N%/% ,%' :#%,+u '%%(5 #%,+u
*)3%'5 )#!($%# +%.'
P)/)$%' :)$%u +%' )$%!% &%+% ,+%*
K%,+u +%.'. !%& #'!)'($%(
+&)$,)* +)'.%' /)'.)#!($%#!
+%.'. !%& !).%$ (%'&% ,)/%#5
+)'.%' >%$% /)$)u! +%,%/ %$
+%' /)'.u%%' #%,+u &%+% !u*u
$)'+%* +%,%/ *%/&% u+%$%
!%/&% ()$)'(u# $)!+u #)'(%,
)$)'(u# &%!(% M%!!%
)$)'(u# &%!(%5 )$3%$'%
>#,%( #)#u''.%' !%/&%
>#,%( (u%5 %u+ %' $%!%
!)&)$( +%.'.5 !)+#( %!%/
K),%$u(%' :,%$u( +%,%/ %$ +'.'
K).u'%%' :!u/)$ &$()' u'(u#
&)$(u/u*%' /#$$.%'!/)P)'"/&%'%' :!/&%' +%,%/ 3%+%* ()$(u(u&
$%&%(5 (+%# ()/u! >%*%"%
D)#!($!% ;Ditjen POM E+! IV5 177 : H%, 900
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S''/ :.,u#!%5 +)#!($!%
RM ? BM :C6H12O6?10516
P)/)$%' :*%,u$ (+%# )$3%$'%5 !)$u#
*%,u! %(%u u($%' &u(*5 (+%#
)$%u5 $%!% /%'!
K),%$u(%' :/u+%* ,%$u( +%,%/ %$5 !%'.%(
/u+%* ,%$u( +%,%/ %$
/)'++*5 %.%# !u#%$ ,%$u(
+%,%/ )(%', ;7<
K).u'%%' :!)%.% !u/)$ 'u($)'( "%'.
!&)!# u'(u# /#$% j%/u$
P)'"/&%'%' :+%,%/ 3%+%* ()$(u(u& %#
BAB III
METODE PRAKTIKUM
A. WAKTU DAN TEMPAT PRAKTIKUM
P$%#(#u/ M#$,. +%' P%$%!(,.
+,%#!%'%#%' &%+% (%'..%, 07 A&$, 2016 &%+% &u#u,
000 !%/&% !),)!% +%' )$()/&%( + L%$%($u/
F%$/%! F%#u,(%! F%$/%! U'=)$!(%! H%,u O,)
B. ALAT DAN BAHAN1 A,%(
A,%(%,%( "%'. +.u'%#%' &%+% &)$>%%' '
%+%,%* !)%.% )$#u( :
% B%(%'. &)'.%+u#
B%(%'. L
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> B(, .),%&
+ B(, !)/&$(
) B(, F%,
f. Buncen burner
. C%3%' &)($
* G),%! #/%
I'#u%($
j. Laminar Air Flow
# O!) u,%(
, P&)( ()()!
/ T%u'. $)%#!
2 B%*%'
A,%(%,%( "%'. +.u'%#%' &%+% &)$>%%' '
%+%,%* !)%.% )$#u( :
% A,#*, 0
L/%* RS B%"%'.#%$%
> L/%* RS K$)/
+ L/%* RS P$%".%
) L/%* RS A,"%*
L/%* RS D)3 S%$(#%
. L/%* RS S%'(% A'%* M)+% &%+%( #%,+u u+%'.
M)+% &%+%( #%,+u +%.'.
j M)+% &%+%( #%'
# S&$(u!
, T!!u)
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3. PROSEDUR KERJA
1.Metode Kuada!
• D!%%' %,%( +%'
%*%'
• D!()$,#%' ,'.#u'.%'
+)'.%' /)'..u'%#%'
%,#*,
• D!()$,#%' !) u,%(
#)+%,%/ %,#*, 0
• D&%'%!#%' >%3%' &)($
/)'..u'%#%' u'!)'
• D&%'%!#%' !)
/)'..u'%#%' !&$(u!
,%,u ++'.'#%'
+)'.%' /)'..!# &%+%
&)'u(u& >%3%' &)($
• D/%!u##%' !) u,%(
#)+%,%/ !%/&), ,%,u
+.$)!#%' &%+%
&)$/u#%%' /)+% &%+%(
!)>%$% .%.
!)%'"%# )/&%( &u(%$%'
• D!/&%' +%,%/
'#u%($ !),%/%
))$%&% j%/
• D%/%(
• Du,%'. *%, "%'. !%/%
&%+% !%/&), ,/%*
KHADIJAH PURNAMASARI AYU RUBAK RERUNG
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L/%* RSB%"%'.#%$%
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RSK$)/ +%' ,/%*
RS S%'(% A'%
H%!, P)'.%/%(%'
".Metode S#$de
• D!%%' %,%( +%'
%*%'
• D!()$,#%' ,'.#u'.%'
+)'.%' /)'..u'%#%'
%,#*, ++%,%/
laminar Air Flow
• D&&)( 9 ()()! !%/&),
,/%* RSB%"%'#%$%
/)'..u'%#%' &&)(
()()! "%'. (),%*
+!()$,!%! #)+%,%/
>%3%' &)($
• D!)%$ !%/&), +%,%/
>%3%' &)($ +)'.%'
/)'..u'%#%' %(%'. L
• D!/&%' +%,%/
'#u%($ !),%/%
))$%&% j%/
• D%/%(
• Du,%'. *%, "%'. !%/%
&%+% !%/&), ,/%*
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L/%* RSB%"%'.#%$%
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1
L/%*
RSB%"%'.#
%$% &%+%
/)+%
&%+%(
u+%'.
M)+% &%+%(
)$u%* 3%$'%
/)'j%+
$%'.)
#)#u''.%'
+)'.%'
/#$% "%'.
(u/u*
)$)'(u#
.$)!%'
.$)!%' .
%.
2 L/%*
RSK$)/
&%+% /)+%
&%+%( ))
M)+% &%+%(
)$u%* 3%$'%
/)'j%+
#)>#,%(%'
+)'.%'
/#$% "%'.
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(u/u*
)$)'(u#
.$)!%'
.$)!%' .
%.
9
L/%*
RSS%'(%
A'% &%+%
/)+%
&%+%( #%'
M)+% &%+%(
)$u%* 3%$'%
/)'j%+
#)#u''.%'
+)'.%'
/#$% "%'.
(u/u*
)$)'(u#
.$)!%'
.$)!%' .
%.
T)#'# S)%$
N
N%/%
S%/&),
G%/%$ K)()$%'.%'
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1
L/%*
RSA,"%*
&%+% /)+%
&%+%( ))
M)+% &%+%(
)$u%* 3%$'%
/)'j%+
#)>#,%(%'
+)'.%'
/#$% "%'.
(u/u*
)$)'(u#
!)%$%'
+)'.%'
/#$% "%'.
)$3%$'%
&u(*
2 L/%*
RSD)3
S%$(#%
&%+% /)+%
&%+%(
M)+% &%+%(
)$u%* 3%$'%
/)'j%+
>#,%(
#)#u''.%'
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u+%'.
+)'.%'
/#$% "%'.
(u/u*
)$)'(u#
!)%$%'
+)'.%'
/#$% "%'.
)$3%$'%
&u(*
9 L/%*
RSP$%".%
&%+% /)+%
&%+%( #%'
M)+% &%+%(
)$u%* 3%$'%
/)'j%+ #$)/
#)#u''.%'
+)'.%'
/#$% "%'.
(u/u*
)$)'(u#
!)%$%'
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+)'.%'
/#$% "%'.
)$3%$'%
&u(*
B. PEMBAHASAN
M#$,. /)$u&%#%' ,/u &)'.)(%*u%' "%'./)/&),%j%$ ()'(%'. $.%'!/) "%'. )$u#u$%'
/#$!#&! %(%u (+%# +%&%( +,*%( +)'.%' #%!%(
/%(% M#$,. /)'>%#u& !(u+ ()'(%'. %#()$5
=$u!5 j%/u$5 &$(%5 ))$%&% .%'..%'. +%'
))$%&% )'(u# #)*+u&%' "%'. (+%# !)!u% u'(u#
+/%!u##%' #)+%,%/ #),/&# ()$!)u( +%(%! B)'(u#
#)*+u&%' "%'. #)>, !)&)$( (u +!)u(
/#$$.%'!/)
I!,%! /)$u&%#%' /)(+) "%'. +.u'%#%' u'(u#
/)/!%*#%' %(%u /)/'+%*#%' /#$% ()$()'(u +%$
!u%(u ,'.#u'.%'5 !)*'..% +&)$,)* #u,(u$ /u$'
%(%u %##%' /u$' Ku,(u$ /u$' %,%* #u,(u$ "%'.
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!),!), /#$%'"% )$%!%, +%$ &)/),%*%' +%$ !%(u
!), (u'..%, B))$%&% >%$% "%'. +,%#u#%' u'(u#
/)'.!,%! /#$$%.%'!/) %'(%$% >%$% .$)!%'
;!treak plate%$% (%u$%'?(u%'. ; pour plate%$% !)%$ ;spread plate%$% &)'.)'>)$%'
;dilution plate< !)$(% />$/%'&u,%($ M#$%
"%'. %#%' +!,%! &%+% &)>%%' ' %+%,%* ,/%*
RSB%"%'.#%$%5 ,/%* RSK$)/5 ,/%* RSS%'(% A'%5
,/%* RSA,"%* +%' ,/%* RSD)3 S%$(#%
I!,%! /#$$.%'!/) u/u/'"% +,%#u#%' +)'.%'
()#'# %!)&(! T)#'# %!)&(! )$(uju%' u'(u#
/)'>).%* %(%u&u' /)'.u$%'. #'(%/'%! "%'. (+%#
+'.'#%'5 #%$)'% !)(%& )'+% "%'. "%'. +.u'%#%'
/%u&u' &)#)$j% ,%$%($u/ +%&%( /)'j%+ !u/)$
#'(%/'%! T)#'# ($%'!)$ %!)&(! %+%,%* !u%(u
/)(+) %(%u ()#'# u'(u# /)/'+%*#%' %(%u
/)'($%'!)$ #u,(u$ %#()$% +%$ !%(u ()/&%( #)
()/&%( ,%' !)>%$% %!)&(! %.%$ (+%# ()$j%+
#'(%/'%! ,)* /#$% ,%' #) +%,%/ #u,(u$
T)#'# ($%'!)$ %!)&(! ' !%'.%( )!)'!%, +%'
#u'> #))$*%!,%' &$!)+u$ />$%, "%'. *%$u!
+#)(%*u ,)* !)$%'. "%'. *)'+%# /),%#u#%'
%'%,!! /#$,.
I!,%! /#$% &%+% &)$>%%' ' +,%#u#%'
+)'.%' >%$% .$)!%' ;!treak plate< +%' +)'.%' >%$%
>%$% !)%$ ;spread plate) Tuju%' u(%/% +%$ >%$%
&)'..$)!%' ' +,%#u#%' +)'.%' /)'..u'%#%' ()#'#
.$)!%' #u%+$%' "%'. /)$u&%#%' ()#'# u'(u#
/)'.*%!,#%' #,'#,' %#()$ "%'. ()$&!%*
+)'.%' %# +%$ !u!&)'! !), "%'. &)#%( C%$% '
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,)* /)'.u'(u'.#%' ,% +('j%u +%$ !u+u(
)#'/ +%' 3%#(u5 (%& /)/)$,u#%' #)()$%/&,%' "%'.
+&)$,)* +)'.%' ,%(*%' P)'..$)!%' "%'. !)/&u$'%
%#%' /)'.*%!,#%' #,' "%'. ()$&!%* C%$%
&)'..$)!%' +%&%( +,%#u#%' +)'.%' >%$% uju'. #%3%(
'#u,%! "%'. /)/%3% %#()$ +.)!)##%' %(%u
+.$)!#%' +)'.%' )'(u# .%. &%+% &)$/u#%%'
%.%$%.%$ +%,%/ >%3%' P)($ !%/&% /),&u( !),u$u*
&)$/u#%%' U'(u# /)/&)$,)* *%!, "%'. %#
+&)$,u#%' #)()$%/&,%'5 "%'. %!%'"% +&)$,)*
+%$ &)'.%,%/%' M)(+) >%3%' .$)! "%'. +,%#u#%'
+)'.%' %# #)%'"%#%' %#%' /)'")%#%'
()$!,%!'"% /#$$.%'!/) "%'. +'.'#%' Du%
/%>%/ #)!%,%*%' "%'. u/u/ !)#%, +,%#u#%' %+%,%*
(+%# /)/%'%%(#%' &)$/u#%%' /)+u/ +)'.%' !)%#
%#'"% u'(u# +.$)! !)*'..% &)'.)'>)$%'
/#$$.%'!/) /)'j%+ #u$%'. ,%'ju( +%' >)'+)$u'.
u'(u# /)'..u'%#%' '#u,u/ ()$,%,u %'"%# !)*'..%
/)'"u,(#%' &)/!%*%' !),!), "%'. +.$)!
C%$% !)%$ +,%#u#%' +)'.%' ()#'# !&+)$
+/%'% ()#'# ' *%$u! +,%#u#%' ++%,%/ laminar
air flow "%'. )$(uju%' u'(u# /)'j%.% !()$,(%!
%,%( +%' %*%' "%'. +.u'%#%' P)$)+%%' %'(%$%
()#'# !&+)$ +%' ()#'# .$)!%' #u%+$%' %+%,%* &%+%
()#'# ' %,%( "%'. +.u'%#%' %+%,%* %(%'. L
!)+%'.#%' &%+% ()#'# .$)!%' #u%+$%' +.u'%#%'
j%$u/ '#u,%! %(%u !) M)+% &%+%( +%,%/ >%3%'
&)($ "%'. (),%* +()()! !%/&), #)/u+%' +!)%$#%'
!)>%$% /)$%(% #)&)$/u#%%' /)+% &%+%( +)'.%'
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/)'..u'%#%' %(%'. L K)u'(u'.%' +%$ ()#'# '
%+%,%* ,)* /u+%* +%,%/ &)$,%#u%''"% +%'+'.#%'
+)'.%' ()#'# .$)!%' #u%+$%' !)+%'.#%' #)#u$%'.%'
+%$ ()#'# ' %+%,%* %"% "%'. +&)$,u#%' ,)*
)!%$ #%$)'% /)'..u'%#%' %,%( Laminar Air Flow.
S)(),%* !%/&), +!,%! #)/u+%' !%/&),
+!/&%' +%,%/ '#u%($ !),%/% #u$%'. ,)* 2 *%$
,%,u +&'+%*#%' #)+%,%/ #u,#%! B)$+%!%$#%'
&)'.%/%(%' &%+% /)+% &%+%( +#)(%*u %*3% %#()$
&%,'. %'"%# (u/u* &%+% /)+% &%+%( +)'.%' ()#'#
!)%$ /)'..u'%#%' %(%'. L +%'+'.#%' +)'.%'
()#'# !)%$ .$)!%' #u%+$%' M)+% &%+%( )$u%*
3%$'% /)'j%+ #u''.#)>#,%(%' +%' /#$% "%'.
(u/u* )$3%$'% &u(* P%+% ()#'# .$)!%' #u%+$%'
/#$% (u/u* /)/)'(u# .%. !)!u% +)'.%'
.$)!%' "%'. +)$#%' P%+% ()#'# !)%$
/)'..u'%#%' %(%'. L /#$% (u/u* !)>%$% /)'")%$
+%' &%,'. %'"%# (u/u* &%+% %.%' &'..$ +%$
>%3%' &)($ P)$)+%%' *%!, ' ++u.% +!)%#%'
()#'# "%'. +.u'%#%' )$)+%5 +/%'% &%+% ()#'#
!)%$ &)$,%#u%' +,%#u#%' ++%,%/ Laminar Air Flow
u'(u# /)'j%.% !()$,!%! +%' %,%( "%'. +.u'%#%'
%+%,%* %(%'. L !)+%'.#%' &%+% ()#'# .$)!
&)$,%#u#%' +,%#u#%' &%+% $u%'. ()$u#% +%'
!()$,!%! +,%#u#%' +)'.%' /)'..u'%#%' %,#*,
!)*'..% ,)* $)'(%' ()$#'(%/'%! %#()$ ,%'
!)$(% %,%( "%'. +.u'%#%' %+%,%* '#u,%! %(%u !)
u,%(
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B+%'. %$/%! !,%! /#$$.%'!/) !%,%*
!%(u'"% +/%'%%(#%' +%,%/ &)/u%(%' =%#!' !u%(u
&)'"%#(
BAB %
PENUTUP
A. KESIMPULAN
B)$+%!%$#%' &$%#(#u/ "%'. (),%* +,%#u#%' /%#%
+%&%( +!/&u,#%' %*3% /#$% ,)* %'"%# (u/u*
&%+% /)+% &%+%( "%'. +!,%! /)'..u'%#%' ()#'#
!)%$ +%'+'.#%' +)'.%' /)'..u'%#%' ()#'# .$)!P%+% ()#'# !)%$ /#$% (u/u* /)'")%$ &%+% >%3%'
&)($ !)+%'.#%' &%+% ()#'# .$)! #u%+$%' /#$%
(u/u* /)/)'(u# .$)!%' .%.
B. SARAN
P$%#(##%' *%$u! /)'"/%# &)'j),%!%' /)'.)'%
&)'.)'%,%' %,%(%,%( %.%$ ()$*'+%$ +%$ *%,*%,
"%'. (+%# +'.'#%' !),%/% &$%#(#u/ )$j%,%'
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DAFTAR PUSTAKA
A+%/5 S5 1772& Dasardasar !ikrobiologi dan Parasitologi
"ntuk Perawat5 EGC5 J%#%$(%
D(j)' POM5 1775 Farmakope #ndonesia E+! IV5
D)&%$()/)' #)!)*%(%' R)&u,# I'+')!%5 J%#%$(%
H%$(5 SA5 201$ !ikrobiologi %ese&atan5 CV A'+
O!)(5 Y."%#%$(%
Pu!&(%!%$5 FD5 M%"%5 S5 +%' N)'.%*5 DK5 20125I!,%! +%' K%$%#()$!%! B%#()$ A)$
P$(),&(# D%$ T%'.# S)&(#5 'urnal (ains dan
(eni #( 5 %o' 1(1)& Su$%%"%
Su(#'3%(5 LI5 20195 M#$% P%$%/)()$ Ku%,(%!
P)$%$%' PP%$ U'(u# U&%"% P)/)!%$%' B(%
Bu++%"%5 'urnal #lmu dan eknologi %elautan ropis5
%o' * (1)& J%#%$(%
Su!, +##5 2005 P)'.%$u* -%#(u +%' Su*u I'#u%! P%+%
O&(/%! A!!%" K( R% M#$%,u/'u$%5 'urnal*adioisotop dan *adiofarmaka5 %o' +5 B%(%'
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