rapd, aflp and rflp analysis
Post on 12-Apr-2017
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MADE BY:PRATEEK KUMAR
RAPD, AFLP AND RFLP ANALYSIS
Marker Gene of known function and location
Gene that allows studying the inheritance of that gene Genetic information resides in the genome
Genetic MarkerAny phenotypic difference controlled by genes,
that can be used for studying recombination processes or selection of a more or less closely
associated target geneAnything in the genome that is variable and can
be used to compare individualsDetectable allelic variation on a chromosome
can be a phenotype, can also be a unique detectable sequence of DNA
Marker is a piece of DNA molecule that is associated with a
certain trait of a organism
What is Marker?
Types of Markers
Animals are selected based on appearance
Disadvantage: lack of polymorphism
Animals are selected based on biochemical properties
Eg. Hb, AMYLASE, BLOOD GROUPS ETC.
Disadvantage: Sex limitedAge dependentInfluenced by environmentIt covers less than 10% of genome
Animals are selected based on structural & numerical
variationsEg. Structural and Numerical VariationsStructural- Deletions, Insertions etc.Numerical- Trisomy, Monosomy, Nullysomy
Disadvantage: low polymorphism
Restriction Fragment Length Polymorphism (RFLP)
Organism can be differentiated by analysis of patterns derived from
cleavage of their DNA.
Technique is mainly based on the special enzyme called Restriction
In RFLP restriction enzyme digested DNA is resolved by gel
electrophoresis and then blotted to a nitro cellulose membrane.
Specific binding pattern can be visualized by hybridization with
Different size or length of restriction fragments are produced such
polymorphism are used to distinguish plant species, genotype etc.