High-Throughput Microscopy High-Throughput Microscopy and Computational/Theoretical and Computational/Theoretical Challenges in the Analysis of Challenges in the Analysis of Neural Circuit StructureNeural Circuit Structure

Society for Neuroscience 2008 MinisymposiumTuesday, November 18, 2008

Room 207B

““The goal of neural science is to The goal of neural science is to understand the mind, how we understand the mind, how we perceive, move, think and remember.”perceive, move, think and remember.”

Eric Kandel, James Schwartz, Thomas Jessell, Eric Kandel, James Schwartz, Thomas Jessell,

Principles of Neural Science, 4th Ed.Principles of Neural Science, 4th Ed.

Santiago Ramón y Cajal

• Considered a founder of Neuroscience

• Used Golgi staining to study the structure of the central nervous system

Sotelo, C. Nature Reviews Neuroscience 4, 7-77, 2003

We know a great deal about how neurons communicate at the level of the individual cell and synapse

http://morbidanatomy.blogspot.com/2008/0/santiago-ramn-y-cajal-1852-934.html

**

**http://www.learner.org/courses/biology/units/neuro/images.html

Cholinergic synaptic vesicle Kandel et al., Principles of Neural Science, 4th Ed.

We know much less about what happens at the network level

Sotelo, C. Nature Reviews Neuroscience 4, 7-77, 2003

http://www.learner.org/courses/biology/ units/neuro/images.html

http://www.learner.org/courses/biology/ units/neuro/images.html http://www.mbl.org/atlas170/atlas 170_start.html

This minisymposium will survey the latest advances in high-throughput microscopy methods with the goal of advancing our understanding of brain networks.

Imaging and analysis of neuronal microcircuits on the scale of whole small animal brains will be presented.

Pertinent computational issues will be discussed.

SpeakersSpeakersSpeakersSpeakers Louise C. Abbott, Texas A&M UniversityLouise C. Abbott, Texas A&M University

Knife-edge scanning microscope (KESM)Knife-edge scanning microscope (KESM) Kristina D. Micheva, Stanford UniversityKristina D. Micheva, Stanford University

Array tomography to probe molecular architecture of neural Array tomography to probe molecular architecture of neural circuitscircuits

Kenneth Hayworth, University of Southern CaliforniaKenneth Hayworth, University of Southern CaliforniaUse of SEM imaging to create ultrathin section librariesUse of SEM imaging to create ultrathin section libraries

Randal A. Koene, Boston UniversityRandal A. Koene, Boston UniversityNETMORPH and automated network reconstructionNETMORPH and automated network reconstruction

Ravi Rao, IBM TJ Watson Research Center, NYRavi Rao, IBM TJ Watson Research Center, NYHigh-performance computing techniques for neuro-image High-performance computing techniques for neuro-image

analysisanalysis Yoonsuck Choe, Texas A&M UniversityYoonsuck Choe, Texas A&M University

Fast algorithms for reconstructing cortical neural circuitsFast algorithms for reconstructing cortical neural circuits

Louise C. Abbott, Texas A&M UniversityLouise C. Abbott, Texas A&M UniversityKnife-edge scanning microscope (KESM)Knife-edge scanning microscope (KESM)

Kristina D. Micheva, Stanford UniversityKristina D. Micheva, Stanford UniversityArray tomography to probe molecular architecture of neural Array tomography to probe molecular architecture of neural

circuitscircuits Kenneth Hayworth, University of Southern CaliforniaKenneth Hayworth, University of Southern California

Use of SEM imaging to create ultrathin section librariesUse of SEM imaging to create ultrathin section libraries Randal A. Koene, Boston UniversityRandal A. Koene, Boston University

NETMORPH and automated network reconstructionNETMORPH and automated network reconstruction Ravi Rao, IBM TJ Watson Research Center, NYRavi Rao, IBM TJ Watson Research Center, NY

High-performance computing techniques for neuro-image High-performance computing techniques for neuro-image analysisanalysis

Yoonsuck Choe, Texas A&M UniversityYoonsuck Choe, Texas A&M UniversityFast algorithms for reconstructing cortical neural circuitsFast algorithms for reconstructing cortical neural circuits

Louise C. AbbottLouise C. Abbott, Texas A&M University, Texas A&M UniversityKnife-edge scanning microscope (KESM)Knife-edge scanning microscope (KESM)

Kristina D. MichevaKristina D. Micheva, Stanford University, Stanford UniversityArray tomography to probe molecular architecture of Array tomography to probe molecular architecture of neural circuitsneural circuits

Kenneth HayworthKenneth Hayworth, University of Southern California, University of Southern CaliforniaUse of SEM imaging to create ultrathin section Use of SEM imaging to create ultrathin section

librarieslibrariesRandal A. KoeneRandal A. Koene, Boston University, Boston University

NETMORPH and automated network reconstructionNETMORPH and automated network reconstructionRavi Rao, Ravi Rao, IBM TJ Watson Research Center, NY

High-performance High-performance computing techniques for neuro-computing techniques for neuro-image image analysisanalysis

Yoonsuck ChoeYoonsuck Choe, Texas A&M University, Texas A&M UniversityFast algorithms for reconstructing cortical neural Fast algorithms for reconstructing cortical neural circuitscircuits

SPEAKERSSPEAKERS