C Y T O M E T R Y S Y M P O S I U M

nd

2 ième SYMPOSIUM DE CYTOMÉTRIE

18 au 20 Juin 2009

th th

June 18 to 20 , 2009

McGill University / Université McGillNew Residence HallMontréal, Québec

www.cytometry.ca©Claudio Calligaris

Thanks you to Academic Core Facilities for support -

Merci aux Plateformes de Service Académiques pour leur soutien

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Welcome to the Canadian Cytometry Assiociation’s 2nd Cytometry Symposium. Visit the CCA’s website (www.cytometry.ca) to find resources on Microscopy and Cytometry and to find out more about the CCA. We wish you an interesting and productive meeting. Bienvenue au 2nd Symposium de Cytométrie organisé par l’Association Canadienne de Cytométrie. Visitez le site Web de l’ACC (www.cytometry.ca) pour trouver des informations sur la microscopie et la cytométrie et pour en savoir plus sur l’ACC. Nous vous souhaitons une conférence intéressante et productive.

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The organizing committee / Le comité organisateur

Laurence Lejeune President CCA – Présidente de l’ACC Manager - Cytometry Facility, CHUM Research Centre-, St-Luc Hospital Responsable - Service de Cytométrie, Centre de Recherche du CHUM, Hôpital Saint Luc

Claire Brown, PhD Scientific Officer CCA –Officier Scientifique ACC Director - Imaging Facility, Life Sciences Complex, McGill University Directrice - Service d’Imagerie, Complexe des Sciences de la Vie, Université McGill

Aleks Spurmanis Microscopy Specialist - Imaging Facility, Life Sciences Complex, McGill University Spécialiste de Microscopie - Service d’Imagerie, Complexe des Sciences de la Vie, Un. McGill

Guillaume Lesage, PhD Administrator - Cell Imaging and Analysis Network, Dept. Biology, McGill University Administrateur - Cell Imaging Analysis Network, Dépt. Biologie, Université McGill

Judith Lacoste, PhD Communication Officer CCA – Officier de communication ACC Microscopy Specialist - CIAN Dept. Biology, McGill University and MIA Cellavie Inc. Spécialiste de Microscopie - CIAN, Dépt. Biologie, Université McGill et MIA Cellavie Inc.

Gabriel Lapointe PhD Candidate - Dept. Biochemistry, University of Montreal Étudiant au Doctorat - Dépt. Biochimie, Université de Montréal

Marie-Hélène Lacombe Treasurer CCA - Trésorière ACC Cytometry Service - Dept. Microbiology and Immunology, McGill University Service de Cytométrie - Dépt. Microbiologie et Immunologie, Université McGill

Christian Young Manager - Flow Cytometry Facility– Lady Davis Institute Responsable du Service de Cytométrie – Institut Lady Davis

Danièle Gagné Communication Officer CCA – Officier de communication ACC Manager - Cytometry Facility, IRIC, University of Montreal Responsable - Service de Cytométrie, IRIC, Université de Montréal

Ken McDonald Flow Cytometry Facility Manager – Life Science Complex, McGill University Responsible Service de Cytométrie – Complexe des Sciences de la vie, Université McGill

Milena Crosato Administrative Coordinator - Segal Cancer Centre research laboratories Coordonnatrice Administrative - Laboratoires de Recherche du Centre du cancer Segal .

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TABLE OF CONTENTS

2009 Cytometry Symposium at a Glance......................................................................4

Agenda .......................................................................................................................5 Thursday, June 18, 2009 ........................................................................................................................................5 Friday, June 19, 2009...............................................................................................................................................5 Saturday, June 20, 2009..........................................................................................................................................7

Room Organization .....................................................................................................9

Abstracts – Oral Presentations ..................................................................................10

Abstracts – Poster Presentations...............................................................................23

Symposium Attendees ..............................................................................................31

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2009 CYTOMETRY SYMPOSIUM AT A GLANCE

Thursday June 18 Friday June 19 Saturday June 20

KEYNOTE LECTURE PLENARY SESSIONS TUTORIAL SESSIONS

08:00

Registration Registration

08:00

09:00

09:00

Introduction to Cytometry Session1: Sample preparation

10:00

Break- Posters - Exhibits Coffee break – Posters – Exhibits

10:00

11:00

11:00

Automated Imaging

12:00

Registration for CFM meeting

Session 2A: Image Collection and Analysis

Session 2B: Flow Cytometry Data Analysis 12:00

13:00 13:00 Lunch – Posters - Exhibits

Lunch – Posters – Exhibits 14:00 14:00

15:00

Core Facility manager meeting

Multiparameter flow cytometry

15:00

Break – Posters - Exhibits

Session 3A: Multi-color Applications

Session 3B: Functional Assays

16:00 Light Refreshments

Closing remarks

16:00

17:00

17:00

18:00 Registration

Watching Biology as it Happens

18:00

19:00 KEYNOTE ADDRESS

N. Labrecque

19:00

20:00 Opening Wine and Cheese

20:00

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AGENDA

Thursday, June 18, 2009

13:00 Core Facility Manager meeting

16:00 Light Refreshments (for Core Facility Manager Meeting attendees) 17:00-19:00 Registration

19:00 KA KEYNOTE ADDRESS

Nathalie Labrecque (Hôpital Maisonneuve-Rosemont, Université de Montréal) Life of a T cell: from precursor to memory

20:00 Opening Wine and Cheese

Friday, June 19, 2009

08:00-17:00 Registration 09:00 Welcome address

Laurence Lejeune, President of the Canadian Cytometry Association

09:10-10:35 Plenary Session 1: Introduction to Cytometry

Sponsored by Media Cybernetics

09:10 O1 Judith Lacoste (CIAN, McGill University and MIA Cellavie)

Introduction to Cytometry

09:30 O2 Laurence Lejeune (Hôpital St-Luc, Université de Montréal) Introduction to Flow Cytometry

09:50 O3 Claire Brown (Life Sciences Complex Imaging Facility, McGill University) Introduction to Light Microscopy

10:10 O4 Arnaud Bonnefoy (Montreal Heart Institute and INSERM U753) Cell Membrane Liquid Order Profiling with Di-4-ANEPPDHQ. Applications to Drug Monitoring, Cell

Activation and Cell Sub-Population Detection

10:35 Coffee Break

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11:00 – 12:30 Plenary Session 2: Automated Imaging: Bridging the Gap between Flow and Microscopy

11:00 O5 Claire Brown (Life Sciences Complex Imaging Facility, McGill University)

Introduction to High Content Screening (HCS)

11:15 O6 Eric Lebel (Olympus Canada Inc.) The New Olympus FV10i Confocal System

11:40 O7 Steve Luke (MDS Analytical Technologies)

Introducing the IsoCyte, the High Speed Laser Scanning Cytometer from MDS Analytical

Technologies

12:05 O8 Mohamed Kodiha (Dept. Physiology, McGill University) Quantitative Immunofluorescence: A novel Tool to Analyze the Subcellular Distribution of Transport

Receptors and Signaling Molecules.

12:30 Lunch (provided thanks to BD Biosciences)

Poster session Vendors exhibit

14:00 CCA General Assembly / Assemblée Générale de l’ACC

14:30-16:10 Plenary Session 3: Development and Future of Multiparameter Flow Cytometry

14:30 O9 Raphael Schneider (Hôpital Notre-Dame, Université de Montréal)

Enhanced Levels of Interleukin-15 in Multiple Sclerosis Contribute to Augmented CD8 T Cell Effector

Functions

14:55 O10 Patricia Ndumbi (Medicine, McGill University)

T-Cell Homeostasis: Implications in HIV Pathogenesis

15:20 O11 Peter Darlington (Montreal Neurological Institute, McGill University) Cytometry with Combinatorial Intracellular Cytokine Staining Reveals Distinct Regulatory Effects of

Natural Killer Cells on Pro-Inflammatory T Cell Responses.

15:45 O12 Greg Finak (Institut de Recherches Cliniques de Montréal) Merging Mixture Model Components for Improved Cell Population Identification in High Throughput

Flow Cytometry Data

16:10 Coffee Break

16:40-18:45 Plenary Session 4: Watching Biology as it Happens

Sponsored by MIA Cellavie Inc.

16:40 O13 Aleks Spurmanis (Life Sciences Complex Imaging Facility, McGill University)

Fundamentals of 3D Fluorescence Microscopy

17:05 O14 Judith Lacoste (CIAN, McGill University and MIA Cellavie Inc.) Live-Cell Microscopy – Tips and Tools

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17:30 O15 Jonas Dorn (IRIC, Université de Montréal) Analysis of CENP-A Replenishment in Early G1

17:55 O16 Haouaria Balghi (McGill University)

Measuring and Visualizing Calcium in Live Cells

18:20 O17 Brian Northan (Media Cybernetics)

Digital Deconvolution of 3 Dimensional Biological Images

Saturday, June 20, 2009

08:00-12:00 Registration

09:00-10:30 Tutorial Session 1: Sample Preparation

09:00 O18 Aleks Spurmanis (Life Sciences Complex Imaging Facility, McGill University)

Essentials of Sample Preparation for Microscopy

09:20 O19 Laurence Lejeune (Hôpital St-Luc, Université de Montréal) Essentials of Sample Preparation for Flow Cytometry

09:40 O20 Gayle Buller (Invitrogen Corp.) Fluorescence, Fluorochromes, and Functional Reagents

10:30 Coffee Break

11:00-12:30 Tutorial Session 2A: Image Collection and Analysis

Sponsored by Photometrics

11:00 O21 Claire Brown (Life Sciences Complex Imaging Facility, McGill University)

What Is an Image?

11:30 O22 Mic Chaudoir (Photometrics) How to Evolve your Imaging

12:00 O23 Gabriel Lapointe (Dépt de Biochimie, Université de Montréal) Image Processing and Analysis

11:00-12:30 Tutorial Session 2B: Flow Cytometry Data Analysis

11:00 024 Bastian R Angermann (Université de Montréal)

Perspectives on Flow Informatics

11:25 O25 David McFarlane (Beckman Coulter Inc.) Kaluza Analysis Software – A New Approach to Flow Cytometry Data Analysis

12:05 O26 Aaron Hart (Tree Star Inc.) Flow Data Analysis, Methods and Techniques

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12:30 Lunch Poster sessions Vendors exhibit

14:30-16:30 Tutorial Session 3A: Multi-color Applications

Sponsored by Carl Zeiss Canada Inc.

14:30 O27 Laurence Lejeune (Hôpital St-Luc, Université de Montréal)

Introduction to Multi-Color Flow Cytometry

15:00 O28 Alexandre Brunet (BD-Biosciences) Introducing the Newest Member of the Family. A Compact Powerful Alternative for your Flow

Cytometry Needs.

15:20 O29 Judith Lacoste (CIAN, McGill University and MIA Cellavie)

Introduction to Co-Localization

16:00 O30 Ian Bates (Carl Zeiss Canada Inc.) Linear Un-Mixing and Spectral Imaging

14:30-16:30 Tutorial Session 3B: Functional Assays

14:30 O31 Dwayne Dexter (Cyntellect Inc.)

High-Throughput In Situ Purification Of Living Cells Using Targeted Laser Ablation

15:00 O32 Sesha Tekur (ThermoFisher Scientific) Utility of High Content Imaging Assays to Address Cell Cycle

15:30 O33 Rich DeMarco (Amnis Corp.) Functional Assays Using ImageStream Cytometry: Image-Based Measurement of Apoptosis, Cell

Cycle and Nuclear Translocation in Flow

16:00 O34 Laurence Lejeune (Hôpital St-Luc, Université de Montréal) Flow Cytometry Approaches to Cell Cycle Analysis, Apoptosis and Cell Proliferation

16:30 Closing remarks

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ROOM ORGANIZATION

Keynote AddressPlenary SessionsTutorial Sessions A

Wine and cheeseSponsors Exhibit

Posters

Tutorial Sessions B

Keynote AddressPlenary SessionsTutorial Sessions A

Wine and cheeseSponsors Exhibit

Posters

Tutorial Sessions B

Keynote AddressPlenary SessionsTutorial Sessions A

Wine and cheeseSponsors Exhibit

Posters

Tutorial Sessions B

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Thank you to our sponsors – Merci à nos commanditaires

2ND CYTOMETRY SYMPOSIUM – 2IÉME SYMPOSIUM DE CYTOMÉTRIE