This data is obtained from the conversion of cellulose to cellobiose by endoglucanase.

[S] 0.5 0.75 2 4 6 8 10Vo 0.075 0.09 0.152 0.196 0.21 0.214 0.23

a) Using the above data, plot a graph on a properly labelled and titledMichaelis-Menten plot.

b) From the graph, calculate the Vmax and Km value of the enzyme.

Vmax = 0.24

= Vmax

= (0.24)

= 0.12

Km = 1.2

Enzyme Kinetics

1. This data is obtained from the conversion of amylopectin to ß-limit dextrin by isoamylase enzyme.

Reaction: amylopectin β-limit dextrin

[S] (mM) Initial velocity (mM.s•1)

2.5 0.588 0.4 1.7011 0.500 1 2.0000.714 0.417 1.4 2.3980.526 0.370 1.9 2.7020.250 0.256 4 3.906

a) Using the above data, recalculate and plot a graph on a properly labelled & titledLineweaver-Burk plot.

b) From the graph, calculate the Vmax and Km value of the enzyme.

Vmax = 1.45 mM s^-1

= Vmax

= (1.45)

= 0.725 mM s^-1

Km = -2.3 mM

c) On the graph above, draw and label the shift in the Lineweaver Burke plotthat would occur if an uncompetitive inhibitor was added to your enzymereaction.

= +

X-intercept: = = -0.435 = -0.44 mM

Y-intercept: = = 0.6897 =0.69 mM s^-1