2695 training
TRANSCRIPT
8/11/2019 2695 Training
http://slidepdf.com/reader/full/2695-training 1/17
0000’’ss
2000 2000
ZQ
1959 1959
GPC
6060’’ss 7070’’ss
1972 1972
M6000
8080’’ss
1980 1980 - - 84 84
Dataadvances
9090’’ss
1996 1996
Alliance2004 2004
8/11/2019 2695 Training
http://slidepdf.com/reader/full/2695-training 2/17
!
"#
$ % #$
& #%#' % ()*()
+, $#% #% %
+ %
%* )$ $
-
. + " /.+"0
8/11/2019 2695 Training
http://slidepdf.com/reader/full/2695-training 3/17
1
2
!" !"
PrimaryPrimary
AccumulatorAccumulator
ABCD
To SampleTo Sample
ManagerManager
EluentsEluents
InIn--LineLine
FilterFilter
DegasserDegasser GPVGPV
InletInlet
C/VC/V
InletInlet
C/VC/V
PressurePressure
TransducerTransducer
PrimaryPrimary
PressurePressure
TransducerTransducer
SystemSystem
8/11/2019 2695 Training
http://slidepdf.com/reader/full/2695-training 4/17
! !
# !$# !$
+
3
!$ !$
8/11/2019 2695 Training
http://slidepdf.com/reader/full/2695-training 5/17
4
% % %!% % %!
Traditional Binary System
Traditional Quaternary System
Alliance System
5 ng of the five peptide testmixture on column can be
easily distinguished from thebaseline in the
chromatograms generated ona Waters Alliance HPLC.
At 5 ng, the peak is lost in thebaseline ripple present in thechromatograms generated on
a competitive “traditional
design” Binary pump andQuaternary gradient system.
5
!$ ! !$ !
5 ! 6 1 ! - #
8/11/2019 2695 Training
http://slidepdf.com/reader/full/2695-training 6/17
55
& '$& '$
241 7 /0
241
+$#+68 9#':,
+ 9, 5-
AA BB CC
5
()()
8/11/2019 2695 Training
http://slidepdf.com/reader/full/2695-training 7/17
5
'$ '$
*++,-
*--..
*++,-,
!/
0#
0#
#0
!!
&
1!
#
#
#
!!
1!
#.
+
+
$
$ 1
0# 1
0# 1
#0 1
!!
!$ 2
314
314
5-
! !! !
; $ 8 ) < ,$
, %
% % <# – $ , ,
8/11/2019 2695 Training
http://slidepdf.com/reader/full/2695-training 8/17
5& 5&
= 6 /= 6 / #%%#%%0 '0 '
52
$ $
, % $$# ## $
> $ ? #%%# ,
8 #* @+6
< ,$$ % , * @ A
#* % % % , $
-1 # $
8 $ %% ? $#
* @+6
B C $$D ,* # , % + ,# %#% # %
$ %
E$ % * # % %
% 5> # %, ,# %
8/11/2019 2695 Training
http://slidepdf.com/reader/full/2695-training 9/17
5
F $ % $ #%* ,
# #
$# % %
A ,
< $% , $ $ %
(% ! , ,$
"# $ $ %% -1 # $
#* %
53
A
12,000
BB
7,0007,000
Same Column
Instrument A --
less Band spreading --
(70µµµµL)MORE PLATES (12,000)
Instrument B --
more Band spreading --
(130µµµµL)LESS PLATES (7,000)
%! 6 $%! 6 $
8/11/2019 2695 Training
http://slidepdf.com/reader/full/2695-training 10/17
54
* / % 7* / % 7
"
+
;!) /9 G 0
EA# "
EA# / ,0
B# "
# &,
EA#
B#
;)$ (
&, # % % % $%+ $ , ! #%, $$#
) 8 , #
8 !8 ! /&$&/&$&
8/11/2019 2695 Training
http://slidepdf.com/reader/full/2695-training 11/17
5
9! / & $$ :: &3; %$ *<#0,= 8&:%4
$9 >/ ??
8&:%& 5&
(PEEK Slip Connectors Easier to Use)
Void
0.090" ParkerStyle
0.130"
WatersStyle
Other Waters® Columns
Waters Spherisorb®,ACQUITY UPLC™and many other brands
9! ( & 99! ( & 9
8/11/2019 2695 Training
http://slidepdf.com/reader/full/2695-training 12/17
8%% % 9 / @!3( & 4 ! A&$&
.020"<.005" .040"
Sample band dispersion inside tubing (ID)
-
/ 9&/ 9&
@% /: $0
@%,# #
o CD# %
+;;= &,
; 88B# @9H
# @H-
88BBH
&@(
@
< % +;;= &,
8/11/2019 2695 Training
http://slidepdf.com/reader/full/2695-training 13/17
1
C& 9!5C& 9!5
;9;9
& @ ? %
@ , # # % , $ , %
% % $
;# $ % % %
F, )
; % #,: $
; * , #
+ %
F, % $ % % , %
% #
<#)#: % @+6 @+H-* 9#
8 #$:)#
2
@@
8# .
& E ,#
o 6 #
8+
BI #%
:
I #% F #
o +)
6
o +&(;
o
8/11/2019 2695 Training
http://slidepdf.com/reader/full/2695-training 14/17
CC
H 6,
&#%#
% #)#$#
: @ H$$#
9 $
E 9
%
o ( * :, %* # %*
$ #
H +
* !
# ;:+
+ # 31> # $
#:#%531@+6
3
6767
8/11/2019 2695 Training
http://slidepdf.com/reader/full/2695-training 15/17
4
* 9!$ 9;7* 9!$ 9;7
< #%#' $ '
9 +#'
"
< # ,,, % % %
,, # , % ###
#
* $$ & 9!$* $$ & 9!$
9;79;7
6 $ % , % $
6 %
< # # % # % 6*
"* * 9 +#'
&% # 7 , %
&% $ ? 7 % #
#
< ' $ #$ * % $
#
8/11/2019 2695 Training
http://slidepdf.com/reader/full/2695-training 16/17
5
9!$ 9; C 9!$ 9; C
D & ( ($ C7D & ( ($ C7
9!$/ @!) % $ ?
7 %
&# % 1
E$ % !# 1 * , #
o , %
o ,, %
o 6' % * * *
#'
F % # #%#' $ # %#,
8/11/2019 2695 Training
http://slidepdf.com/reader/full/2695-training 17/17
D & ( ($ C7D & ( ($ C7
:#%
$ # % '
&# % > $ % # #%#'
E$ % %%* % ,
E$ % * % % ' $ %
% #' % %% ,
% ' %
? ) $ # %
# #%#'
-
C!!&&C!!&&
! $ 9;! $ 9;
5 % $ % <# #
& * #
%#' % $$# $
- F $ #% + % % %
1 & $ % % #
& $ /2#
2 + % A#
+ ; $ %*
J < ! $ ?,