abeer sterilization

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Author: mstfa-mgdy

Post on 14-Apr-2017



Health & Medicine

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  • Hepatitis B, Hepatitis C, & human immunodeficiency virus (HIV).

    It is generally accepted that the potential for HBV transmission in the dental environment is greater than that for HIV.

  • Clean: Absence of all matter in which micro-org. may find a favourable condition for growth.

    Contamination: Presence of an infectious agent. (m-organism capable of producing disease)


  • Germicide: Causing death of micro-organism.

    Bactericidal: Causing the death of bacteria.

    Bacteriostatic: Inhibition of bacterial growth


  • Disinfection: Destruction of pathogenic agents. ( < than sterilization )

    Sterilization: Destruction of all forms of life.Terminology

  • Objectives of infection control

    Never introduce microbes into the root canal system during endo. ttt.

    Decrease the N. of existing microbes to that normal body resistance can prevent infection.

    Prevent cross-infection disease.

  • Cleaning Disinfection Sterilization

  • Is 1st step where debris can be mechanical removed How ? 1- Instruments should be wiped in alcohol immediately.


  • 2- Scrubbing gross debris on instrument with detergent in warm water using a BRUSH. Ordinary Soap..............? alkaline film

    3- An Ultrasonic Cleaner can be used in addition to hand scrubbing.

  • A-Chemical solutions:

    B-Boiling and steam:


  • A-Chemical solutions: For thermo labile materials Gutta-Percha

    Soln. Glutraldehyde, Iodophors Soln, HIV & HBV.Conc. changed regularly, in closed container Disinfectant cycle starts, the addition of contaminated instruments would disrupt the procedure.Corrosion of instruments & Pt. sensitivity

    Surface disinf. by NaOCL, Alcohols, Ammonium cop.

  • B-Boiling and steam:

    Not capable of destroying all bacteria and spores and inefficient against Hepatitis virus.

    They can not be used for sterilization.

  • 1-Autoclaving2- Dry heat3-Chemical vapour 4-Ethylene oxide gas5- Salt (Glass) bead Sterilizers6- Flaming


  • 1- Autoclaving:

    special closed system100% steamPressure 1-2.15 bar.Temp. 121-134 C Time: 3-30 min.

  • AutoclavingThe disadvantage (pressurized steam) are - Erosion of glass surface; - corrosive effect on sharp metal instruments, - Not suitable for paper, oils, powders and cotton

  • AutoclavingChecking Temperature

    Brownes tube

    Sterilizing bag

  • 2- Dry heat:

    At 160 C for 60-90 min.suitable for sharp instruments, paper points, oils, and powders.

    it is less effective for handpieces and rubber.

  • 3- Chemical vapour *Formaldehyde and alcohol with heat and pressure.

    *Temp: 137 C under 1-1.5 bar pressure

    *It is effective in killing all organisms * The corrosion is eliminated

    *. Slightly longer times than the autoclaves.

  • 4- Ethylene oxide gas:For heat labile materials .Powders, plastics, delicate sharp instruments, motors & hand-pieces Conc.: 450 mg/litre.Temp: 60 C is used Time: several hours Porous materials absorb some of the gas.

  • 5- Salt (Glass) bead Sterilizers:

    This method is rapid & on bracket T. & just before RCT., not affect the sharp cutting edge

    At 218 C for 5-10 sec

    Used to sterilize:

    Instruments tips.filesSilver cones & Paper points cotton pellets (will chare off if kept in the sterilizer too long).

  • 6- Flaming:

    Effective only at actually touches. Ruin the cutting edge of the instruments.

    Nowadays, not commonly used.

  • BARRIERS:Gloves:Eliminate cross-contamination.Proper fit --->>> Tactile sensation.Disposable.Double gloves indicated for pt. with known infectious disease eg: Herpes ; HIV ; HBV.

  • Hand Washing:Before & after gloves are used.

    Face Masks ; Eye Glasses and/or Face Shields.

    Clothing: Clean gowns ; head covers ; shoes.

  • Procedural Barriers:

    Rubber dam. High suction. Operatory equipments: Disposable paper; plastic; or Al. wrap to cover surfaces and.Syringe: Sterilizable or disposable.

  • Sharp Instruments: After use ; all sharps should be placed into puncture-resistant containers >>> then disposed.

    Immunization: Vaccines ->>> HBV.