deliverables: peptide mapping - acroscale · 2017. 12. 21. · deliverables: peptide mapping...
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http://www.acro-scale.co.jp/Acroscale Inc. Sendai laboratory +81-22-752-2211 [email protected]
D E L I V E R A B L E S : P E P T I D E M A P P I N G
Enz ymatic d igest ion , LC/MS measurements and analys is per formed
Measurement ser v ice de l iverable
Chromatogram: T IC (U V )
(E�ciency and sample amount cannot be guaranteed)
CharacterizeLC / MS
P r e - p r o c e s s i n g s e r v i c e M e a s u r e m e n t A n a l y s i s s e r v i c e
Chromatogram / Spectra
F u l l c h r o m a t o g r a m
Our measurement se r v ice prov ides chromatograms and spectra as R AW data
EnzymatictreatmentDenature
ReportRaw dataGlycoproteinPeptide
Glycopeptide
Del iverables
A n a l y s i s s e r v i c eS equence ID Repor tR AW dataProcessed sample
(Chromatogram, Spectra, fragment labeling (sequence identification), coverage, etc analysis to identify the di�erent parts of the sequence)
A n a l y s i s s e r v i c eGlycosylation Site Report
Estimation of glycosylation sites(Inspection of di�erent locations on the sequence
and estimation of glycosylation site(s))
S p e c t r a
X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X
http://www.acro-scale.co.jp/Acroscale Inc. Sendai laboratory +81-22-752-2211 [email protected]
D E L I V E R A B L E S : P E P T I D E M A P P I N G
Analys is ser v ice de l iverable: Sequence identification
F u l l c h r o m a t o g r a m : T I C
Our analysis service includes chromatogram, spectra, fragment labeling (sequence identification), coverage, etc analysis to identify the di�erent parts of the sequence.
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Peptide fragmentidentification
Analys is ser v ice de l iverable: Est imation of g lycosy lat ion pos it ion
Full chromatogram comparison: TIC
Amino acid sequence coverage:
Di�erent sites on the sequence inspected and glycosylation site(s) estimated
Non-g l ycos y lated p rote in
G l ycos y lated p rote in
Chromatogramcomparison
# # %
Fr a g m e n t l a b e l i n g
S p e c t r a