QUANTITATIVE INFORMATION FOR SPIROS AGATHOS, Ph.D.

EDUCATION

Massachusetts Institute of Technology (MIT) Ph.D. 1983 Biochemical Engineering

McGill University M. Eng. 1976 Chemical Engineering

National Technical University of Athens Eng. 1973 Chemical Engineering

1. List of theses (Ph.D, and/ or MS) supervised

Rutgers – The State University of New Jersey

1. Jaegwan Lee, Ph.D., August 1989, “Physiological Factors in Cyclosporin A Production by Tolypocladium inflatum – Experimental and Mathematical Modeling”. Current position: Professor, Choong Ang University, Ansung, Republic of Korea

2. Gie-Taek Chun, Ph.D., January 1992, “Comparative Bioreactor Studies of Suspended and Immobilized Cell Systems for Optimal Production of Cyclosporin A” Current position: Professor, Department of Molecular Bioscience, Kangwon National University, Chuncheon, Republic of Korea

3. Eock-Kee Hong, Ph.D., January 1992, “Plasmid Propagation and Heterologous Gene Expression in Recombinant Yeast” (Co-supervisor: A. Constantinides). Current position: Professor, Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Republic of Korea

4. William T. Hensler, Ph.D., January 1994, “Growth, Infectivity, and Recombinant Protein

Expression in Insect Cell Culture”. Current position: Consultant, Facility Logix, Inc., Fulton, MD, U. S. A.

Catholic University of Louvain

5. Saïd El Fantroussi, Ph.D., May 1997, “Introduction of Anaerobic Bioremediation Activities in Soil: From Laboratory Bench Scale to Pilot Soil Bioreactor” (Co-supervisor: H. Naveau). Current position: Private sector, import/export and commodity trading, Brussels, Belgium

6. Caroline M. Perès, Ph.D., November 1998, “Improved Biodegradation of Nitroaromatic Compounds: Degradation of a Nitroaromatic Compound together with the Aminoaromatic Derivative Produced by its Reduction”. Current position: Senior Scientist, Danisco Genencor, Palo Alto, CA, U. S. A.

7. Victor Alejandro Achà Fuertes, Ph.D., June 1999, “Development of a FTIR Sensor for Monitoring and Modelling an Anaerobic Dechlorinating Bioreactor” (Co-supervisor: H. Naveau). Current position: Assistant Professor, Institut Polytechnique LaSalle Beauvais, Beauvais, France

8. Sébastien Goux, Ph.D., March 2000, “Biodegradation of Atrazine by Soil Microorganisms: Molecular Aspects and Potential Use in Bioremediation” (Co-supervisor: L. Pussemier). Current position: Science officer, European Commission, Brussels, Belgium

9. Benoît Van Aken, Ph.D., November 2000, “Biodegradation of 2,4,6-Trinitrotoluene (TNT) by

White-Rot Fungi”. Current position: Assistant Professor, Temple University, Philadelphia, PA, U. S. A.

10. Lacina Coulibaly, Ph.D., January 2002, “Bioconversion of macromolecules in a reactor simulating plug flow in transient regime: case of bioremediation of synthetic wastewater by Aspergillus niger”. Current position : Vice-President, Université d’Abobo-Adjamé, Abidjan, Ivory Coast

11. Laertis Ikonomou, Ph.D., June 2002, “Development of High Density Systems for the Production of Recombinant Proteins by Insect Cells” (Co-supervisor: G. Bastin). Current position: Assistant Professor, Boston University, Boston, MA, U. S. A.

12. Johann Mols, Ph.D., July 2004. “Cultivation of Chinese Hamster Ovary (CHO) Cells in Protein- Free Media Supplemented with Plant Peptones is Sufficient to Prevent Proteolysis of Secreted Rcombinant Glycoproteins” (Co-supervisor: Y.-J. Schneider). Current position: Research Scientist, GlaxoSmithKline Biologicals, Rixensart, Belgium

13. Jan Dries, Ph.D., September 2004. “Development and Comparisonon of Different Multiple

Permeable Reactive Barrier (MPRB) Concepts for the Treatment of Groundwater Contaminated by

Pollutant Mixtures” (Co-supervisors: L. Bastiaens & D. Springael). Current position: Associate Professor, University of Antwerp, Hoboken, Belgium

14. Laurent Eyers, Ph.D., January 2007. “Characterization of Bacterial Populations of 2,4,6-

Trinitrotoluene (TNT) Contaminated Soils and Isolation of a Pseudomonas aeruginosa Strain with TNT

Denitration Activities”. Current position: New technology specialist, Procter & Gamble, Geneva, Switzerland

15. Jean-Christophe Drugmand, Ph.D., February 2007. “Characterization of Insect Cell Lines Is Required for Appropriate Industrial Processes – Case Study of High-Five Cells for Recombinant Protein Production” (Co-supervisor: Y.-J. Schneider). Current position: R&D Engineer, ATMI Life Sciences Division, Pall Corporation, Brussels, Belgium

16. Luc Schuler, Ph.D., December 2007. “Identification of Genes Involved in the Degradation of Polycyclic Aromatic Hydrocarbons by Two Sphingomonas spp”. Current position: Forensic Genetics Officer, Division of Law Enforcement, Judiciary Police, Luxembourg

17. Hubert Cabana, Joint Ph.D. (Catholic University of Louvain and University of Sherbrooke, Québec, Canada), April 2008. “Elimination of the endocrine disruptors nonylphenol, bisphenol A and triclosan by the oxidative action of the laccase from Coriolopsis polyzona” (Co-supervisor: J.P. Jones). Current position: Associate Professor, University of Sherbrooke, Sherbrooke, Québec, Canada

18. Benoît Stenuit, Ph.D., November 2009. “Biological degradation of 2,4,6 – Trinitrotoluene (TNT): Bioprospecting for TNT-denitrating bacteria and deciphering of multiple TNT denitration pathways”. Current position: Postdoctoral fellow, Université Catholique de Louvain, Belgium

19. Jean-François Michiels, Ph.D., May 2011. “Investigation of the role of peptones in animal cell culture: Case study of a commercial soy peptone in a CHO cell serum-free medium” (Co-supervisor: Y.-J. Schneider). Current position:Junior Biostatistician, Arlenda, Charleroi area, Belgium

20. Sébastien Sart, Ph.D., August 2011. “Controlled expansion and differentiation of mesenchymal stem cells in microcarrier-based stirred bioreactors” (Co-supervisor: Y.-J. Schneider). Current position: Postdoctoral fellow, Ecole Polytechnique, Paris, France

21. Leticia Isabel Ramirez Cavazos, Ph.D., May 2014. “Thermostable laccases from Pycnoporus sanguineus CS43, production enhancement, purification and characterization” (Co-supervisor: Roberto Parra Saldivar, Instituto Tecnologico de Monterrey, Mexico). Current position: Postdoctoral researcher, Centro del Agua, Instituto Tecnologico de Monterrey, Mexico

22. Philippe Demarche, Ph.D., May 2014. “Immobilized phenoloxidases in reactors for the oxidation of aquatic contaminants of emerging concern. Towards a scalable industrial system”. Current position: Postdoctoral researcher, Université Catholique de Louvain, Belgium

23. Borhane Samir Grama, Ph.D., June 2014. “Production of high added value molecules by microalgae isolated from the Algerian Sahara: optimization of culture conditions and of induction parameters” (Co-supervisor: Douadi Khelifi, University of Constantine I, Algeria). Current position: Postdoctoral researcher, Université Catholique de Louvain, Belgium

2. List of publications (at list 100)

A. Articles in International Peer-Reviewed Journals

1. S.N. Agathos, A.L. Griffith, R. Uauy-Dagach, V.R. Young and N. Catsimpoolas

(1977).

Transient Velocity Sedimentation at Unit Gravity of Human Erythrocytes. Anal. Biochem.

81: 143-150.

2. A.L. Demain, A. Poirier, S. Agathos and O. Nimi (1981). Appearance and Disappearance of

Gramicidin S Synthetases. Devel. Ind. Microbiol. 22: 233-244.

3. S.N. Agathos and A.L. Demain (1983). Optimization of Fermentation Processes through the

Control of In Vivo Inactivation of Microbial Biosynthetic Enzymes. ACS Symp.

Ser. 207

(Foundations of Biochemical Engineering): 53-67.

4. S.N. Agathos and A.L. Demain (1984). Gramicidin S Synthetase Stabilization In Vivo. Ann.

N.Y. Acad. Sci. 434: 44-47.

5. A.L. Demain and S.N. Agathos (1986). Studies on In Vivo Inactivation of Gramicidin S

Synthetase and its Retardation. Can. J. Microbiol. 32: 208-214.

6. S.N. Agathos and A.L. Demain (1986). Substrate Amino Acid-Mediated Stabilization of

Gramicidin S Synthetase against Inactivation In Vivo. Enzyme Microb. Technol. 8:

465-468.

7. S.N. Agathos and A.L. Demain (1986). Dissolved oxygen levels and the in vivo stability of

gramicidin S synthetase. Appl. Microbiol. Biotechnol. 24: 319-322.

8. S.N. Agathos, J.W. Marshall, C. Moraiti, R. Parekh and C. Madhosingh (1986).

Physiological and Genetic Factors for Process Development of Cyclosporine Fermentations.

J. Ind. Microbiol. 1: 39-48.

9. S.N. Agathos, C. Madhosingh, J.W. Marshall and J. Lee (1987). The Fungal Production of

Cyclosporine. Ann. N.Y. Acad. Sci. 506: 657-662.

10. J. Lee and S.N. Agathos (1989). Effect of Amino Acids on the Production of Cyclosporin A

by Tolypocladium inflatum. Biotechnol. Lett. 11: 77-82.

11. G-T. Chun and S.N. Agathos (1989). Immobilization of Tolypocladium inflatum Spores into

Porous Celite Beads for Cyclosporine Production. J. Biotechnol. 9: 237-254.

12. J. Tasker and S.N. Agathos (1989). Dependence of In Vivo Inactivation Kinetics of

Gramicidin S Synthetase on Cell Physiology. Biotechnol. Bioeng. 34:1037-1044.

13. T.H. Aarnio and S.N. Agathos (1989). Production of Extracellular Enzymes and Cyclosporin

by Tolypocladium inflatum and Morphologically Related Fungi. Biotechnol. Lett.

11: 759-

764.

14. S.N. Agathos and R. Parekh (1990). Enhancement of Cyclosporin Production in a

Tolypocladium inflatum Strain after Epichlorohydrin Treatment. J. Biotechnol. 13: 73-

81.

15. S.N. Agathos, Y-H. Jeong and K. Venkat (1990). Growth Kinetics of Free and Immobilized

Insect Cell Cultures. Ann. N.Y. Acad. Sci. 589: 372-398.

16. T.H. Aarnio and S.N. Agathos (1990). Pigmented Variants of Tolypocladium inflatum in

Relation to Cyclosporin Production. Appl. Microbiol. Biotechnol. 33: 435-437.

17. G-T. Chun and S.N. Agathos (1991). Comparative Studies of Physiological and

Environmental Effects on the Production of Cyclosporin A in Suspended and Immobilized

Cells of Tolypocladium inflatum. Biotechnol. Bioeng. 37: 256-265.

18. J. Lee and S.N. Agathos (1991). Dynamics of L-valine in Relation to the Production of

Cyclosporin A by Tolypocladium inflatum. Appl. Microbiol. Biotechnol. 34: 513-517.

19. S.N. Agathos (1991). Production Scale Insect Cell Culture. Biotechnol. Adv. 9: 51-68.

20. S.N. Agathos and J. Lee (1993). Mathematical Modeling of the Fungal Production of

Cyclosporin A: Effect of L-valine, Biotechnol. Progress, 9: 54-63.

21. G-T. Chun and S.N. Agathos (1993). Dynamic Response of Immobilized Cells to Pulse

Addition of L-valine in Cyclosporin A Biosynthesis, J. Biotechnol., 27: 283-294.

22. A. Nathan, S. Zalipsky, S.I. Ertel, S.N. Agathos, M.L. Yarmush and J. Kohn

(1993).

Copolymers of Lysine and Polyethylene Glycol: A New Family of Functionalized Drug-

Carriers, Bioconjugate Chem., 4: 54-62.

23. W. Hensler, V. Singh and S.N. Agathos (1994). Sf9 Insect Cell Growth and β-Galactosidase

Production in Serum and Serum Free Media, Ann. N.Y. Acad. Sci., 745: 149-166.

24. W.T. Hensler and S.N. Agathos (1994). Evaluation of Monitoring Approaches and Effects of

Culture Conditions on Recombinant Protein Production in Baculovirus-Infected Insect Cells,

Cytotechnology, 15: 177-186.

25. J.-B. Boucquey, P. Renard, P. Amerlynck, P. Modesto Filho, S.N. Agathos, H. Naveau and E.-J. Nyns (1995). High-Rate Continuous Biodegradation of Concentrated Chlorinated Aliphatics by a Durable Enrichment of Methanogenic Origin under Carrier-Dependent

Conditions, Biotechnol. Bioeng. 47: 298-307.

26. S.N. Agathos (1996). Insect Cell Bioreactors, Cytotechnology 20: 173-189.

27. S. El Fantroussi, J. Mahillon, H. Naveau and S.N. Agathos (1997). Introduction of Anaerobic

Dechlorinating Bacteria in Soil Slurry Microcosms and Nested PCR Monitoring. Appl.

Environ. Microbiol. 63: 806-811.

28. B. Van Aken, K. Skubisz, H. Naveau and S.N. Agathos (1997). Biodegradation of 2,4,6- Trinitrotoluene (TNT) by the White-Rot Basidiomycete Phlebia radiata. Biotechnol. Lett.

19: 813-817.

29. S. El Fantroussi, J. Mahillon, H. Naveau and S.N. Agathos (1997). Introduction and PCR

Detection of Desulfomonile tiedjei in Soil Slurry Microcosms. Biodegradation 8: 125-133.

30. D. Dochain, S.N. Agathos and P. Vanrolleghem (1997). Asymptotic Observers as a Tool for

Modelling Process Dynamics. Water Sci. Technol. 36: 259-268.

31. S.N. Agathos, E. Hellin, H. Ali-Khodja, S. Deseveaux, F. Vandermesse and H. Naveau

(1997). Gas-Phase Methyl Ethyl Ketone Biodegradation in a Tubular Biofilm Reactor:

Microbiological and Bioprocess Aspects. Biodegradation, 8: 251-264.

32. S. El Fantroussi, R. Giot, H. Naveau and S.N. Agathos (1998). Acclimation of a

Methanogenic Consortium to a Mixture of Hydroxylated Aromatic Compounds.

Chemosphere, 36: 1575-1583.

33. S. El Fantroussi, H. Naveau and S.N. Agathos (1998). Anaerobic Dechlorinating Bacteria.

Biotechnol. Progr. 14: 167-188.

34. C.M. Peres, H. Naveau and S.N. Agathos (1998). Biodegradation of Nitrobenzene by

Simultaneous Reduction of Nitrobenzene into Aniline and Mineralization of the Aniline

Formed. Appl. Microbiol. Biotechnol. 49: 343-349.

35. S. El Fantroussi, E. Ntibahezwa, S. Thomas, H. Naveau and S.N. Agathos (1998). Effects of

Specific Inhibitors on the Anaerobic Reductive Dechlorination of 2,4,6-Trichlorophenol by a

Stable Methanogenic Consortium. Anaerobe 4: 197-203.

36. S. Goux, S.N. Agathos and L. Pussemier (1998). Metabolic Characterization of Fifteen

Atrazine Degrading Microbial Communities. J. Ind. Microbiol. Biotechnol. 21: 254-259.

37. B. Van Aken, L. Godefroid, C. Peres, H. Naveau and S.N. Agathos (1999). Mineralization of

14C-U-Ring Labeled 4-Hydroxylamino-2,6-Dinitrotoluene by Manganese-

Dependent

Peroxidase of the White-Rot Basidiomycete Phlebia radiata. J. Biotechnol. 68: 159-169.

38. P. Ledent, H. Michels, G. Blackman, H. Naveau and S.N. Agathos (1999). Reversal of the

Inhibitory Effect of Surfactants upon Germination and Growth of a Consortium of Different

Strains of Bacillus. Appl. Microbiol. Biotechnol. 51: 370-374.

39. B. Van Aken, M. Hofrichter, K. Scheibner, A.I. Hatakka, H. Naveau and S.N. Agathos

(1999). Transformation and Mineralization of 2,4,6-Trinitrotoluene (TNT) by Manganese

Peroxidase from the White-Rot Basidiomycete Phlebia radiata. Biodegradation 10: 83-

91.

40. S. El Fantroussi, M. Belkacemi, E.M. Top, J. Mahillon, H. Naveau and S.N. Agathos (1999).

Bioaugmentation of a Soil Bioreactor Designed for Pilot Scale Anaerobic Bioremediation

Studies. Environ. Sci. Technol. 33: 2992-3001.

41. C.M. Peres, B. Van Aken, H. Naveau and S.N. Agathos (1999). Continuous Degradation

of Mixtures of 4-Nitrobenzoate and 4-Aminobenzoate by Immobilized Cells of

Burkholderia cepacia strain PB4. Appl. Microbiol. Biotechnol. 52: 440-445.

42. V. Acha, M. Meurens, H. Naveau, D. Dochain, G. Bastin and S.N. Agathos (1999). Model- Based Estimation of an Anaerobic Reductive Dechlorination Process via an ATR-FTIR

(Attenuated Total Reflection-Fourier Transform Infrared) Sensor. Water Sci. Technol. 40:

33-40.

43. V. Acha, M. Meurens, H. Naveau and S.N. Agathos (1999). Detoxification of a Mixture of Aliphatic Chlorinated Hydrocarbons in a Fixed-Bed Bioreactor: Continuous On-Line Monitoring via an ATR-FTIR (Attenuated Total Reflection-Fourier Transform Infrared)

Sensor. Water Sci. Technol. 40: 41-47.

44. M.R. Kosseva, V.N. Beschkov, C. Peres and S.N. Agathos (1999) Removal of 2,4- Dinitrophenol by Simultaneous Adsorption and Bacteria Pseudomonas putida Fixed on Solid

Supports. Bulg. Chem. Comm. 31: 45-50.

45. B. Van Aken, P. Ledent, H. Naveau and S.N. Agathos (2000). Co-immobilization of

Manganese Peroxidase from Phlebia radiata and Glucose Oxidase from Aspergillus niger on

Porous Silica Beads. Biotechnol. Lett. 22: 641-646.

46. V. Acha, M. Meurens, H. Naveau and S.N. Agathos (2000). ATR-FTIR Sensor Development for Continuous On-line Monitoring of Chlorinated Aliphatic Hydrocarbons in a Fixed-Bed

Bioreactor. Biotechnol. Bioeng. 68: 473-487.

47. C.M. Peres and S.N. Agathos (2000). Biodegradation of Nitroaromatic Pollutants: from

Pathways to Remediation. Biotechnology Annual Review, 6: 197-220

48. B. Van Aken, J.D. Stahl, H. Naveau, S.N. Agathos and S.D. Aust (2000). Transformation of

2,4,6-Trinitrotoluene (TNT) Reduction Products by Lignin Peroxidase (H8) from the White-

Rot Basidiomycete Phanerochaete chrysosporium. Bioremediation Journal, 4: 135-145.

49. T. Patarinska, D. Dochain, S.N. Agathos and L. Ganovski (2000). Modelling of Continuous

Microbial Cultivation Taking into Account the Memory Effects. Bioproc. Eng. 22: 517-

527.

50. S.J. Goux, M. Ibanez, M. Van Hoorick, P. Debongnie, S.N. Agathos and L. Pussemier

(2000). Biodegradation of Atrazine in Sand Sediments and in a Sand-Filter. Appl. Microbiol.

Biotechnol. 54: 589-596.

51. B. Van Aken, M.D. Cameron, J.D. Stahl, A. Plumat, H. Naveau, S.D. Aust and S.N.

Agathos (2000). Glutathione-Mediated Mineralization of 14

C-Labeled 2-Amino-4,6-Dinitrotoluene by Manganese-Dependent Peroxidase H5 from the White-Rot Fungus Phanerochaete chrysosporium. Appl. Microbiol. Biotechnol. 54: 659-664.

52. P. Wattiau, M.-E. Renard, P. Ledent, V. Debois, G. Blackman and S.N. Agathos (2001). A PCR Test to Identify Bacillus subtilis and Closely Related Species and Its Application to the

Monitoring of Wastewater Biotreatment. Appl. Microbiol. Biotechnol. 56: 816-819.

53. R. Borraccino, M. Kharoune, R. Giot, S.N. Agathos, E.-J. Nyns, H.P. Naveau and A. Pauss

(2001). Abiotic transformation of catechol and 1-naphthol in aqueous solution - Influence of

environmental factors. Water Research, 35: 3729-3737.

54. B. Van Aken and S.N. Agathos (2001). Biodegradation of nitro-substituted explosives by

ligninolytic white-rot fungi: A mechanistic approach. Adv. Appl. Microbiol. 48: 1-77.

55. C. M. Peres, R. Russ, H. Lenke and S. N. Agathos (2001). Biodegradation of 4-nitrobenzoate,

4-aminobenzoate and their mixtures: new strains, unusual metabolites and insights into

pathway regulation. FEMS Microbiol. Ecol. 37: 151-159.

56. L. Ikonomou, G. Bastin, Y.-J. Schneider and S.N. Agathos (2001). Design of an Efficient

Medium for Insect Cell Growth and Recombinant Protein Production. In Vitro Cell. Develop.

Biol. - Animal, 37: 549-559.

57. G.-T. Chun and S.N. Agathos (2001). Application of Oxygen Uptake Rate Measured by a

Dynamic Method for Analysis of Related Fermentation Parameters in Cyclosporin A

Fermentation: Suspended and Immobilized Cell Cultures. J. Microbiol. Biotechnol. 11:

1055-

1060.

58. B. Van Aken and S.N. Agathos (2002). Implication of Manganese (III), Oxalate, and Oxygen in the Degradation of Nitroaromatic Compounds by Manganese Peroxidase (MnP). Appl.

Microbiol. Biotechnol. 58: 345-351.

59. I. Sokolovska, P. Wattiau, P. Gerin and S.N. Agathos (2002). Biodegradation of Fluorene at

Low Temperature by a Psychrotrophic Sphingomonas sp. Chem. Pap. 56: 36-40.

60. L. Ikonomou, C. Peeters-Joris, Y.-J. Schneider and S. N. Agathos (2002). Supernatant

Proteolytic Activities of High-Five Insect Cells Grown in Serum-Free Culture. Biotechnol.

Lett. 24: 965-969.

61. D. Wesenberg, F. Buchon and S.N. Agathos (2002). Degradation of Dye-Containing Textile

Effluent by the Agaric White-Rot Fungal Strain Clitocybula dusenii. Biotechnol. Lett.

24:

989-993.

62. R. El Mamouni, R. Jacquet, P. Gerin and S.N. Agathos (2002). Influence of Electron Donors and Acceptors on the Bioremediation of Soil Contaminated with Trichloroethene and Nickel:

Laboratory and Pilot-Scale Study. Water Sci. Technol. 45: 49-54.

63. O. Drzyzga, R. El Mamouni, S.N. Agathos and J.C. Gottschal (2002). Dehalogenation of

Chlorinated Ethenes and Immobilization of Nickel in Anaerobic Sediment Columns under

Sulfidogenic Conditions. Environ. Sci. Technol. 36: 2630-2635.

64. L. Coulibaly, H. Naveau and S.N. Agathos (2002). A tanks-in-series bioreactor to simulate macromolecular-laden wastewater pretreatment under sewer conditions by Aspergillus niger.

Water Res. 36: 3941-3948.

65. P. Wattiau, D. Springael, S.N. Agathos and S. Wuertz (2002). Use of the pAL5000 Replicon in PAH-Degrading Mycobacteria: Application for Strain Labelling and Promoter Probing.

Appl. Microbiol. Biotechnol. 59: 700-705.

66. L. Ikonomou, J.-C. Drugmand, G. Bastin, Y.-J. Schneider and S. N. Agathos (2002).

Microcarrier Culture of Lepidopteran Cell Lines: Implications for Growth and Recombinant

Protein Production. Biotechnol. Progr. 18: 1345-1355.

67. S.J. Goux, N. Shapir, S. El Fantroussi, S. Lelong, S.N. Agathos and L. Pussemier (2003).

Long-term maintenance of rapid atrazine degradation in soils inoculated with atrazine

degraders. Water, Air & Soil Pollution: Focus, 3: 131-142.

68. L. Ikonomou, Y.-J. Schneider and S. N. Agathos (2003). Insect cell culture for industrial

production of recombinant proteins. Appl. Microbiol. Biotechnol., 62: 1-20.

69. D. Wesenberg, I. Kyriakides and S.N. Agathos (2003). White-rot fungi and their enzymes for

the treatment of industrial dye effluents. Biotechnol. Adv. 22: 161-187.

70. L. Coulibaly and S.N. Agathos (2003). Transformation kinetics of mixed polymeric

substrates under transitory conditions by Aspergillus niger. Afr. J. Biotechnol. 2: 438-

443.

71. I. Sokolovska, R. Rozenberg, C. Riez, P.G. Rouxhet, S.N. Agathos and P. Wattiau (2003).

Carbon source-induced modifications in the mycolic acid content and cell wall permeability

of Rhodococcus erythropolis E1. Appl. Environ. Microbiol. 69: 7019-7027.

72. C.C. Burteau, F.R. Verhoeye, J.F. Mols, J.S. Ballez, S.N. Agathos and Y.J. Schneider (2003).

Fortification of a protein-free cell culture medium with plant peptones improves cultivation and productivity of an interferon-gamma producing CHO cell line. In Vitro Cell. Develop.

Biol. - Animal, 39: 291-296.

73. L. Coulibaly G. Gourene and S.N. Agathos (2003). Utilization of fungi for biotreatment of

raw wastewater. Afr. J. Biotechnol. 2: 620-630.

74. V. Beschkov, S. Velizarov, S.N. Agathos and V. Lukova (2004). Bacterial denitrification of

waste water stimulated by constant electric field. Biochem. Eng. J., 17: 141-145.

75. J. Dries, L. Bastiaens, D. Springael, S.N. Agathos and L. Diels (2004). Competition for sorption and degradation of chlorinated ethenes in batch zero-valent iron systems. Environ.

Sci. Technol. 38: 2879-2884.

76. J. Mols, C. Peeters-Joris, S.N. Agathos and Y.-J. Schneider (2004). Origin of rice

protein hydrolysates added to protein-free media alters secretion and extracellular

proteolysis of recombinant interferon-gamma as well as CHO-320 cell growth.

Biotechnol. Lett. 26: 1043-

1046.

77. L. Ikonomou, G. Bastin, Y.-J. Schneider and S.N. Agathos (2004). Effect of partial medium replacement on cell growth and protein production in the insect cell-baculovirus system.

Cytotechnology, 44: 67-76.

78. L. Eyers, I. George, L. Schuler, B. Stenuit, S.N. Agathos and S. El Fantroussi (2004).

Environmental genomics: exploring the unmined richness of microbes to degrade

xenobiotics. Appl. Microbiol. Biotechnol. 66: 123-130.

79. J.S. Ballez, J. Mols, C.C. Burteau, S.N. Agathos and Y.J. Schneider (2004). Plant protein hydrolysates support CHO-320 cells proliferation and recombinant interferon-gamma

production in suspension and inside microcarriers in protein-free media. Cytotechnology, 44:

103-114.

80. S. El Fantroussi and S.N. Agathos (2005). Is bioaugmentation a feasible strategy for pollutant

removal and site remediation? Curr. Opin. Microbiol. 8: 268-275.

81. J. Mols, C. Peeters-Joris, R. Wattiez, S.N. Agathos and Y.-J. Schneider (2005). Recombinant interferon-gamma secreted by CHO-320 cells cultivated in protein-free media is protected against extracellular proteolysis by the expression of natural protease inhibitors and by the addition of plant protein hydrolysates to the culture medium. In Vitro Cell. Develop. Biol. -

Animal, 41: 83-91.

82. B. Stenuit, L. Eyers, S. El Fantroussi and S.N. Agathos (2005). Promising strategies for the

mineralization of 2,4,6-trinitrotoluene. Rev. Environ. Sci. Biotechnol. 4: 39-60.

83. J. Dries, L. Bastiaens, D. Springael, S. Kuypers, S.N. Agathos and L. Diels (2005). Effect of humic acids on heavy metal removal by zero-valent iron in batch and continuous flow

columns. Water Research, 39: 3531-3540.

84. J. Dries, L. Bastiaens, D. Springael, S.N. Agathos and L. Diels (2005). Combined removal of chlorinated ethenes and heavy metals by zerovalent iron in batch and continuous flow

column systems. Environ. Sci. Technol. 39: 8460-8465.

85. H. Ali-Khodja, H. Naveau and S.N. Agathos (2005). The removal of methyl ethyl ketone in an essentially gaseous-phase biofilm bioreactor: aspects of dynamic behaviour and the

influence of operating characteristics. Fresenius Environ. Bull. 14: 709-715.

86. L. Coulibaly and S.N. Agathos (2006). Influence of Aspergillus niger inoculum size upon the kinetics of macromolecule degradation under transient operating conditions. Eur. J. Sci. Res.

15: 38-51.

87. L. Eyers, Smoot, J.C., Smoot, L.M., Bugli, C., Urakawa, H., McMurry, Z., Siripong, S., El Fantroussi, S., Lambert, P., Agathos, S.N., and Stahl, D.A. (2006). Discrimination of shifts in a soil microbial community associated with TNT-contamination using functional ANOVA of

16S rRNA hybridized to oligonucleotide microarrays. Environ. Sci. Technol. 40: 5867-5873.

88. A. Provost, G. Bastin, S.N. Agathos, Y.-J. Schneider (2006). Metabolic design of macroscopic bioreaction models: application to Chinese hamster ovary cells. Bioprocess

Biosyst. Eng. 29: 349-366.

89. B. Stenuit, L. Eyers, R. Rozenberg, J.-L. Habib-Jiwan and S.N. Agathos (2006). Aerobic growth of Escherichia coli on 2,4,6-trinitrotoluene (TNT) as sole nitrogen source and evidence of TNT denitration by whole cells and cell-free extracts. Appl. Environ. Microbiol.

72: 7945-7948.

90. H. Cabana, J.-L. Habib Jiwan, R. Rozenberg, V. Elisashvili, M. Penninckx, S.N. Agathos and J.P. Jones (2007). Elimination of endocrine disrupting chemicals nonylphenol and bisphenol A and personal care product ingredient triclosan using enzyme preparation from the white rot

fungus Coriolopsis polyzona. Chemosphere 67: 770-778.

91. H. Cabana, J.P. Jones and S.N. Agathos (2007). Preparation and characterization of

cross- linked laccase aggregates and their application to the elimination of endocrine

disrupting chemicals. J. Biotechnol. 132: 23-31.

92. H. Cabana, J.P. Jones and S.N. Agathos (2007). Elimination of endocrine disrupting chemicals using white rot fungi and their lignin modifying enzymes: A review. Engineering

in Life Sciences, 7: 429-456.

93. L. Coulibaly and S.N. Agathos (2007). Effects of Aspergillus niger inoculum concentration upon the kinetics of starchy wastewater pretreatment in a tanks-in-series bioreactor under

transitory conditions. Brazilian Journal of Chemical Engineering 24: 499-507.

94. S. Chader, H. Hacene, M. Belhamel and S. Agathos (2007). Etudes des procédés de

production biologiques de l’hydrogène. Revue des Energies Renouvelables 10: 497-505.

95. S. Vanhulle, M. Trovaslet, E. Enaud, M. Lucas, S. Taghavi, D. Van der Lelie, B. Van Aken, M. Foret, R.C.A. Onderwater, D. Wesenberg, S.N. Agathos, Y.-J. Schneider and A.-M.

Corbisier (2008). Decolourisation, cytotoxicity and genotoxicity reduction during a combined ozonation/fungal treatment of dye contaminated wastewater. Environ. Sci.

Technol. 42: 584-589.

96. L. Schuler, S.M. Ní Chadhain, Y. Jouanneau, C. Meyer, G.J. Zylstra, P. Hols and S.N.

Agathos (2008). Characterization of a novel angular dioxygenase from fluorene-degrading

Sphingomonas sp. strain LB126. Appl. Environ. Microbiol. 74: 1050-1057.

97. I. George, L. Eyers, S. El-Fantroussi and S.N. Agathos (2008). Effect of 2,4,6-trinitrotoluene

on soil bacterial communities. J. Ind. Microbiol. Biotechnol. 35: 225-236.

98. L. Eyers, B. Stenuit and S.N. Agathos (2008). Denitration of 2,4,6-trinitrotoluene by

Pseudomonas aeruginosa ESA-5 in the presence of ferrihydrite. Appl. Microbiol.

Biotechnol. 79: 489-497.

99. B. Stenuit, L. Eyers, L. Schuler, S.N. Agathos and I. George (2008). Emerging high- throughput approaches to analyze bioremediation of sites contaminated with hazardous

and/or recalcitrant wastes. Biotechnol. Advances 26: 561-575.

100. B. Stenuit and S.N. Agathos (2009). Rapid and unbiased colorimetric quantification of nitrite and ammonium ions released from 2,4,6-trinitrotoluene during biodegradation studies:

Eliminating interferences. Int. Biodeter. Biodegr. 63: 116-122.

101. V. Elisashvili, E. Kachlishvili, N. Tsiklauri, E. Metreveli, T. Khardziani and S.N. Agathos

(2009). Lignocellulose-degrading enzyme production by white-rot Basidiomycetes isolated

from the forests of Georgia. World J. Microbiol. Biotechnol. 25: 331-339.

102. S. Sart, Y.J. Schneider and S.N. Agathos (2009). Ear mesenchymal stem cells: An efficient

adult multipotent cell population fit for rapid and scalable expansion. J. Biotechnol.

139:

291-299.

103. B. Stenuit, L. Eyers, R. Rozenberg, J.L. Habib Jiwan, S. Matthijs, P. Cornelis and S.N.

Agathos (2009). Denitration of 2,4,6-trinitrotoluene in aqueous solutions using small- molecular-weight catalyst(s) secreted by Pseudomonas aeruginosa ESA-5. Environ. Sci.

Technol. 43: 2011-2017.

104. H. Cabana, J.P. Jones and S.N. Agathos (2009). Utilization of cross-linked laccase aggregates in a perfusion basket reactor for the continuous elimination of endocrine

disrupting chemicals. Biotechnol. Bioeng. 102: 1582-1592.

105. H. Cabana, C. Alexandre, S.N. Agathos and J. P. Jones (2009). Immobilization of the laccase from the white-rot fungus Coriolopsis polyzona and use of the immobilized biocatalyst for

the continuous elimination of endocrine disrupting chemicals. Bioresour. Technol. 100:

3447-3458.

106. L. Schuler, Y. Jouanneau, S.M. Ní Chadhain, C. Meyer, M. Pouli, G.J. Zylstra, P. Hols and S.N. Agathos (2009). Characterization of a ring-hydroxylating dioxygenase from phenanthrene-degrading Sphingomonas sp. strain LH128 able to oxidize benz[a]anthracene.

Appl. Microbiol. Biotechnol. 83: 465-475.

107. S. Chader, H. Hachene and S.N. Agathos (2009). Study of hydrogen production by

three strains of Chlorella isolated from the soil in the Algerian Sahara. International

Journal of Hydrogen Energy 34: 4941-4946.

108. I. George, M.R. Liles, M. Hartmann, W. Ludwig, R.M. Goodman and S.N. Agathos (2009).

Changes in soil Acidobacteria communities after 2,4,6-trinitrotoluene contamination. FEMS

Microbiol. Lett.296: 159-166.

109. V. Elisashvili, E. Kachlishvili, T. Khardziani and S.N. Agathos (2010). Effect of aromatic compounds on the production of laccase and manganese peroxidase by white-rot

basidiomycetes. J. Ind. Microbiol. Biotechnol. 37: 1091-1096.

110. S. Sart, Y.J. Schneider and S.N. Agathos (2010). Influence of culture parameters on ear

mesenchymal stem cells expanded on microcarriers. J. Biotechnol. 150: 149-160.

111. B. Stenuit and S.N. Agathos (2010). Microbial 2,4,6-trinitrotoluene degradation: could we learn from (bio)chemistry for bioremediation and vice versa? Appl. Microbiol. Biotechnol.

88: 1043-1064.

112. J.-F. Michiels, J. Barbau, S. De Boel, S. Dessy, S.N. Agathos and Y.-J. Schneider (2011).

Characterization of beneficial and detrimental effects of a soy peptone as an additive for

CHO cell cultivation. Process Biochem. 46: 671-681.

113. R. Taboada-Puig, C. Junghanns, P. Demarche, M.T. Moreira, G. Feijoo, J.M. Lema and S.N.

Agathos (2011). Combined cross-linked enzyme aggregates from versatile peroxidase and glucose oxidase: Production, partial characterization and application for the elimination of

endocrine disruptors. Bioresource Technol. 102: 6593-6599.

114. J.-F. Michiels, S. Sart, Y.-J. Schneider and S.N. Agathos (2011). Effects of a soy peptone on

gamma-IFN production steps in CH-320 cells. Process Biochem. 46: 1759-1766.

115. I.F. George, M. Hartmann, M.R. Liles and S.N. Agathos (2011). Recovery of as-yet

uncultured soil Acidobacteria on dilute soil media. Appl. Environ. Microbiol. 77: 8184-

8188.

116. P. Demarche, C. Junghanns, R. Nair and S.N. Agathos (2012). Harnessing the power of

enzymes for environmental stewardship. Biotechnology Advances 30: 933-953.

117. J.-C. Drugmand, Y.-J. Schneider and S.N. Agathos (2012). Insect cells as factories for

biomanufacturing. Biotechnology Advances 30: 1140-1157.

118. P. Demarche, C. Junghanns, N. Mazy and S.N. Agathos (2012). Design-of-experiment strategy for the formulation of laccase biocatalysts and their application to degrade bisphenol

A. New Biotechnology 30: 96-103.

119. B. Stenuit, G. Lamblin, P. Cornelis and S.N. Agathos (2012). Aerobic denitration of 2,4,6- trinitrotoluene in the presence of phenazine compounds and reduced pyridine nucleotides.

Environ. Sci. Technol. 46: 10605-10613.

120. C. Arboleda, H. Cabana, E. De Pril, J.P. Jones, G.A. Jimenez, A.I. Mejia, S.N. Agathos and M.J. Penninckx (2013). Elimination of bisphenol A and triclosan using the enzymatic system of autochthonous Colombian forest fungi. ISRN Biotechnology, online journal Article ID

96824, http://dx.doi.org/10.5402/2013/968241

121. S. Sart, A. Errachid, Y.-J. Schneider and S.N.Agathos (2013). Modulation of mesenchymal stem cell actin organization on conventional microcarriers for proliferation and differentiation in stirred bioreactors. J. Tissue Eng. Regen. Med. 7: 537-551 (Published on line 2 March 2012 DOI: 10.1002/term.545).

122. R.R. Nair, P. Demarche and S.N. Agathos (2013). Formulation and characterization of an immobilized laccase biocatalyst and its application to eliminate organic micropollutants in

wastewater. New Biotechnology, 30: 815-823.

123. P.M. Murray, S. Moane, C. Collins, T. Beletskaya, O.P. Thomas, A.W.F. Duarte, F.S. Nobre, I.O. Owoyemi, F.C. Pagnocca, L.D. Sette, E. McHugh, E. Causse, P. Pérez-López, G. Feijoo, M.T. Moreira, J. Rubiolo, M. Leirós, L.M. Botana, S. Pinteus, C. Alves, A. Horta, R.

Pedrosa, C. Jeffryes, S.N. Agathos, C. Allewaert, A. Verween, W. Vyverman, I. Laptev, S. Sineoky, A. Bisio, R. Manconi, F. Ledda, M. Marchi, R. Pronzato and D.J. Walsh (2013).

Sustainable production of biologically active molecules of marine based origin.

New

Biotechnology, 30: 839-

850.

124. S. Sart, S.N. Agathos and Y. Li (2013). Engineering stem cell fate with biochemical and

biomechanical properties of microcarriers. Biotechnology Progress 29: 1354-

1366.

125. P. Pérez-López, S. González-García, C. Jeffryes, S.N. Agathos, E. McHugh, D. Walsh, P.

Murray, S. Moane, G. Feijoo and M.T. Moreira (2014). Life cycle assessment of the production of the red antioxidant carotenoid astaxanthin by microalgae: from lab to pilot

scale. Journal of Cleaner Production, 64: 332-344 (Published on line 18 July 2013, DOI:

http://dx.doi.org/10.1016/j.jclepro.2013.07.011).

126. B.S. Grama, S. Chader, D. Khelifi, S.N. Agathos and C. Jeffryes (2014). Induction of canthaxanthin production in a Dactylococcus microalga isolated from the Algerian Sahara.

Bioresource Technology, 151: 297-305.

127. S. Sart, S.N. Agathos and Y. Li (2014). Process engineering of stem cell metabolism for large

scale expansion and differentiation in bioreactors. Biochemical Engineering Journal, 84:

74-

82.

128. M. Gavrilescu, K. Demnerová, J. Aamand, S. Agathos and F. Fava (2014). Emerging pollutants in the environment: present and future challenges in biomonitoring, ecological risks and bioremediation. New Biotechnology, in press (published on line 21 January 2014, DOI 10.1016/j.nbt.2014.01.001 http://dx.doi.org/10.1016/j.nbt.2014.01.001).

129. S. Sart, Y.-J. Schneider, Y. Li and S.N. Agathos (2014). Stem cell bioprocess engineering towards cGMP production and clinical applications. Cytotechnology, in press (published on line 06 February 2014, DOI 10.1007/s10616-013-9687-7 http://dx.doi.org/10.1007/s10616-

013-9687-7).

130. M. Majone, R. Verdini, F. Aulenta, S. Rossetti, V. Tandoi, N. Kalogerakis, S. Agathos, S.

Puig, G. Zanaroli and F. Fava (2014). In situ groundwater and sediment bioremediation: barriers and perspectives at European contaminated sites. New Biotechnology, in press (published on line 06 March 2014, DOI 10.1016/j.nbt.2014.02.011 http://dx.doi.org/10.1016/j.nbt.2014.02.011).

131. L.I. Ramírez-Cavazos, C. Junghanns, R. Nair, D.L. Cárdenas-Chávez, C. Hernández-Luna, S.N. Agathos and R. Parra (2014). Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design. J. Zheijiang Univ-Sci B (Biomed & Biotechnol) 15(4): 343-352.

132. J. Li, M. Stamato, E. Velliou, C. Jeffryes and S.N. Agathos (2014) Design and characterization of a scalable airlift flat panel photobioreactor for microalgae cultivation. Journal of Applied Phycology, in press (published on line 22 May 2014, DOI

10.1007/s10811-014-0335-1 http://dx.doi.org/10.1007/s10811-014-0335-1).

133. L.I. Ramírez-Cavazos, C. Junghanns, N. Ornelas-Soto, D.L. Cárdenas-Chávez, C. Hernández- Luna, P. Demarche, E. Enaud, R. García-Morales, S.N. Agathos and R. Parra (2014). Purification and characterization of two thermostable laccases from Pycnoporus sanguineus and potential role in degradation of endocrine disrupting chemicals. Journal of Molecular Catalysis B: Enzymatic, 108: 32-42 (published on line 02 July 2014, DOI

10.1016/j.molcatb.2014.06.006 http://dx.doi.org/10.1016/j.molcatb.2014.06.006).

134. B.S. Grama, S. Chader, D. Khelifi, B. Stenuit, C. Jeffryes and S.N. Agathos (2014).

Characterization of fatty acid and carotenoid production in an Acutodesmus microalga isolated from the Algerian Sahara. Biomass and Bioenergy 69: 265-275 (published on line 17

August 2014, DOI: 10.1016/j.biombioe.2014.07.023 http://dx.doi.org/10.1016/j.biombioe.2014.07.023).

B. Book Chapters

135. S.N. Agathos and A.L. Demain (1988). The In Vivo Longevity of Antibiotic Synthetases. In: S. Aiba, Editor, Horizons of Biochemical Engineering, pp. 147-161, Oxford University Press, Oxford/New York.

136. S.N. Agathos (1991). Mass Production of Viral Insecticides. In: K. Maramorosch, Editor, Biotechnology for Biological Control of Pests and Vectors, pp. 217-235, CRC Press, Boca Raton, Florida.

137. S.N. Agathos (1992). Insect Cell Culture. In: A.I. Laskin, Editor, Biotechnology International

1990/1991, Century Press, London.

138. S.N. Agathos (1993). The Future of Insect Cell Culture Engineering. In: M.F.A. Goosen, A.J.

Daugulis and P. Faulkner, Editors, Insect Cell Culture Engineering, pp. 218-237, Marcel

Dekker, New York.

139. S.N. Agathos (1994). Large Scale Insect Cell Production. In: K. Maramorosch and A.H.

McIntosh, Editors, Insect Cell Biotechnology, pp. 89-103, CRC Press, Boca Raton, FL.

140. S.N. Agathos (1996). Insect Cell Bioreactors. In: J.M. Vlak, C.D. de Gooijer, J. Tramper & H.G. Miltenburger, Editors, Insect Cell Cultures: Fundamental and Applied Aspects, pp.

173-189, Kluwer Academic Publishers, Dordrecht. ISBN 0-7923-3403-5.

141. T.H. Lee, G.T. Chun, Y.K. Chang, J.D. Lee and S.N. Agathos (1996) Bioprocess Engineering Considerations in Cyclosporin A Fermentation by Immobilized Fungus Tolypocladium inflatum. In: R.H. Wijffels, R.M. Buitelaar, C. Bucke & J. Tramper, Editors, Immobilized Cells: Basics and Applications, pp. 402-409, Elsevier Science, Amsterdam.

ISBN 0-444-81984-3. (Progess in Biotechnology, Volume 11).

142. S.N. Agathos (1997). Environmental Biotechnology in Research and Education. In: C.

Ronneau and O. Bitchaeva, Editors, Biotechnology for Waste Management and Site

Restoration, pp. 33-39, Kluwer Academic Publishers, Dordrecht. ISBN 0-7923-4769-2.

143. V. Achà, M. Meurens, H. Naveau and S.N. Agathos (1998). Continuous On-Line Monitoring of Organochlorine Compounds in a Fixed Bed Bioreactor by an Optical Infrared Sensor. In: Bioremediation and Phytoremediation -- Chlorinated and Recalcitrant Compounds (Wickramanayake, G.B. and Hinchee, R.E. Ed.), pp. 135-141, Battelle Press, Columbus, Ohio.

144. C.M. Peres, H. Naveau and S.N. Agathos (1999). Cross Induction of 4-Nitrobenzoate and 4- Aminobenzoate Degradation by Burkholderia cepacia strain PB4. In: R. Fass, Y. Flashner and S. Reuveny, Editors, Novel Approaches for Bioremediation of Organic Pollution, pp. 71-

82, Plenum, New York. ISBN 0-306-46102-1.

145. L. Ikonomou, G. Bastin, Y.-J. Schneider and S.N. Agathos (1999). Growth on Microcarriers and Nutritional Needs of High Density Insect Cell Cultures. In: A. Bernard, B. Griffiths, W. Noé and F. Wurm, Editors, Animal Cell Technology: Products from Cells, Cells as Products, pp. 371-373, Kluwer Academic Publishers, Dordrecht. ISBN 0-7923-6075-3.

146. G. Baré, H. Charlier, L. De Nijs, F. Verhoeye, Y.-J. Schneider, S. Agathos and P. Thonart (2001). Effects of Rice Protein Hydrolysates on Growth of CHO Cells and Production of Human Interferon-gama in Serum-Free Medium. In: E. Lindner-Olsson, N. Chatzissavidou and E. Lüllau, Editors, Animal Cell Technology: From Target to Market, pp. 217-219, Kluwer Academic Publishers, Dordrecht. ISBN 1-4020-0264-5.

147. J. Mols, C. Burteau, F. Verhoeye, C. Peeters-Joris, G. Bastin, S.N. Agathos and Y.-J.

Schneider (2001). Proteolytic Potential During Batch Cultivation in Serum-Free Media of an IFN-gamma Producing CHO Cell Line. In: E. Lindner-Olsson, N. Chatzissavidou

and E. Lüllau, Editors, Animal Cell Technology: From Target to Market, pp. 227-229, Kluwer

Academic Publishers, Dordrecht. ISBN 1-4020-0264-5.

148. J.-C. Drugmand, L. Ikonomou, Y.-J. Schneider and S.N. Agathos (2001). Growth of High- Five Cells on Cultispher-S Microcarriers. In: E. Lindner-Olsson, N. Chatzissavidou and E.

Lüllau, Editors, Animal Cell Technology: From Target to Market, pp. 332-334,

Kluwer

Academic Publishers, Dordrecht. ISBN 1-4020-0264-5.

149. L. Ikonomou, J.-C. Drugmand, G. Bastin, Y.-J. Schneider and S.N. Agathos (2001).

Physiology Effect of Insect Cells Cultured in a New Serum-Free Medium. In: E. Lindner- Olsson, N. Chatzissavidou and E. Lüllau, Editors, Animal Cell Technology: From Target to Market, pp. 338-340, Kluwer Academic Publishers, Dordrecht. ISBN 1-4020-0264-5.

150. F. Verhoeye, C. Burteau, J. Mols, J.-S. Ballez, G. Baré, P. Thonart, G. Bastin, S.N. Agathos and Y.-J. Schneider (2001). Use of Plant Peptone-Containing Serum-Free Media for the Cultivation of CHO Cells in Suspension and on Microcarriers. In: E. Lindner-Olsson, N. Chatzissavidou and E. Lüllau, Editors, Animal Cell Technology: From Target to Market, pp.

362-364, Kluwer Academic Publishers, Dordrecht. ISBN 1-4020-0264-5.

151. F. Verhoeye, C. Burteau, S. Chenu, J.-L. Goergen, A. Marc, G. Bastin, S.N. Agathos and Y.- J. Schneider (2001). Adaptation and Growth Kinetics of the Universal Host CHO Cell Lines

in Serum-Free Medium. In: E. Lindner-Olsson, N. Chatzissavidou and E. Lüllau, Editors, Animal Cell Technology: From Target to Market, pp. 438-440, Kluwer Academic Publishers, Dordrecht. ISBN 1-4020-0264-5.

152. S.N. Agathos and W. Reineke (2002). Preface. In: S.N. Agathos & W. Reineke, Editors, Biotechnology for the Environment: Strategy and Fundamentals. Focus on Biotechnology Series, Volume 3a, pp. v-vi, Kluwer Academic Publishers, Dordrecht, The Netherlands, 248 pp. ISBN 1-4020-0529-6.

153. S. Goux, S.N. Agathos and L. Pussemier (2002). Towards a Better Understanding of Enhanced Pesticide Biodegradation. In: S.N. Agathos and W. Reineke, Editors, Biotechnology for the Environment: Stategy and Fundamentals. Focus on Biotechnology Series, Volume 3a, Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 141-156.

ISBN 1-4020-0529-6.

154. S.N. Agathos and W. Reineke (2002). Preface. In: S.N. Agathos & W. Reineke, Editors, Biotechnology for the Environment: Soil Remediation. Focus on Biotechnology

Series, Volume 3b, pp. 1-2, Kluwer Academic Publishers, Dordrecht, The Netherlands, 148 pp. ISBN 1-4020-1051-6.

155. J. Dries, L. Bastiaens, D. Springael D, S.N. Agathos and L. Diels (2002). Kinetics of trichloroethene (TCE) reduction by zero valent iron: effect of medium composition. In: S.F. Thornton & S.E. Oswald, Editors, Groundwater Quality: Natural and Enhanced Restoration of Groundwater Pollution, IAHS Publication no. 275, pp. 397-402. IAHS Press, Wallingford, Oxforshire, UK. ISBN 1-901502-86-4.

156. J. Dries, L. Bastiaens, D. Springael, S.N. Agathos and L. Diels (2002). Combined removal of chlorinated ethenes and heavy metals in zero valent iron systems. In: S.F. Thornton & S.E.

Oswald, Editors, Groundwater Quality: Natural and Enhanced Restoration of Groundwater Pollution, IAHS Publication no. 275, pp. 447-452. IAHS Press, Wallingford, Oxforshire, UK. ISBN 1-901502-86-4.

157. S.N. Agathos and W. Reineke (2003). Preface. In: S.N. Agathos & W. Reineke, Editors, Biotechnology for the Environment: Wastewater Treatment and Modeling, Waste Gas Handling. Focus on Biotechnology Series, Volume 3c, pp. 1-2, Kluwer Academic Publishers, Dordrecht, The Netherlands, 288 pp. ISBN 1-4020-1131-8.

158. L. Eyers, S.N. Agathos and S. El Fantroussi (2004). Denaturing gradient gel electrophoresis (DGGE) as a fingerprinting tool for analyzing microbial communities in contaminated environments. In: J.F.T. Spencer and A.L. Ragout de Spencer, Editors, Environmental Microbiology: Methods and Protocols. Methods in Biotechnology Series, Volume 16, pp.

407-417, Humana Press, Totowa, New Jersey. ISBN 1-58829-116-2.

159. J.C. Drugmand, Y.J. Schneider and S.N. Agathos (2005). Environmental effects of lactate on High-Five

TM insect cell metabolism. In: F. Gòdia and M. Fussenegger, Editors, Animal

Cell Technology Meets Genomics, pp. 91-94, Springer, Dordrecht. ISBN 1-4020-2791-5.

160. J. Mols, F. Verhoeye, C. Peeters-Joris, S.N. Agathos and Y.J. Schneider (2005). Extracellular metallopreoteases and their inhibitors expressed by the IFN-gamma producing CHO-320 cell line. In: F. Gòdia and M. Fussenegger, Editors, Animal Cell Technology Meets Genomics, pp.

175-177, Springer, Dordrecht. ISBN 1-4020-2791-5.

161. S.N. Agathos (2005). Bioaugmentation as a strategy for in situ bioremediation. In: F. Fava and P. Canepa, Editors, Innovative Approaches to the Bioremediation of Contaminated Sites, pp. 291-300, INCA, Venice, Italy. ISBN 88-88214-33-X.

162. S.N. Agathos (2006). Chlorinated solvents biodegradation and nickel bioimmobilization in historically contaminated anaerobic sediments. In: P. Canepa and F. Fava, Editors, Biomonitoring, Bioavailability and Microbial Transformation of Pollutants in Sediments and Approaches to Stimulate their Biodegradation, pp. 33-44, Soil Remediation Series N° 7, INCA, Venice, Italy. ISBN 88-88214-32-1.

163. S. El Fantroussi, S.N. Agathos, D.H. Pieper, R. Witzig, B. Camara, L. Gabriel-Jürgens; H.

Junca, G. Zanaroli, F. Fava, J.R. Perez-Jimenez, L.Y. Young, K. Hamonts, R. Lookman, M. Maesen, L. Diels, W. Dejonghe, J. Dijk and D. Springael (2006). Biological assessment and remediation of contaminated sediments. In: D. Reible and T. Lanczos, Editors, Assessment and Remediation of Contaminated Sediments, pp. 179-238, NATO Science Series, IV. Earth and Environmental Sciences – Vol. 73, Springer, Dordrecht, The Netherlands. ISBN-10 1-

4020-4958-7.

164. F. Fava and S.N. Agathos (2006). Uncertainty and research needs in the area of biological restoration of contaminated sediments. In: D. Reible and T. Lanczos, Editors, Assessment and Remediation of Contaminated Sediments, pp. 239-246, NATO Science Series, IV. Earth and Environmental Sciences – Vol. 73, Springer, Dordrecht, The Netherlands. ISBN-10 1-

4020-4958-7.

165. J. Mols, N. Cavez, S. Agathos and Y.-J. Schneider (2007). Plant peptones: Nutritional properties sustaining recombinant protein secretion by CHO cells. In: R. Smith, Editor, Cell Technology for Cell Products, pp. 599-601, Springer, Dordrecht. ISBN 978-1-4020-5475-4.

166. J.-C. Drugmand, Y.-J. Schneider and S. Agathos (2007). Analysis and modeling of the metabolism of lepidopteran cells. In: R. Smith, Editor, Cell Technology for Cell Products, pp. 777-780, Springer, Dordrecht. ISBN 978-1-4020-5475-4.

167. J.-C. Drugmand, J.-F. Michiels, S. Agathos and Y.-J. Schneider (2007). Growth of mammalian and lepidopteran cells on BioNOC II disks, a novel macroporous microcarrier. In: R. Smith, Editor, Cell Technology for Cell Products, pp. 781-784, Springer, Dordrecht. ISBN 978-1-4020-5475-4.

168. Agathos, S. N. (2007). Development of serum-free media for lepidopteran insect cell lines.

In: D. W. Murhammer, Editor, Baculovirus Expression Protocols, second edition, Methods in Molecular Biology Series, Volume 388, pp. 155-185. Humana Press, Totowa, New Jersey.

ISBN 978-1-58829-537-8.

169. B. Stenuit, L. Eyers, S.N. Agathos, I. George (2008). High-throughput approaches to analyse waste biotreatment in confined environments. In: Microbial Biodegradation: Genomics and Molecular Biology, E. Diaz (ed.), pp. 319-358. Caister Academic Press, Norwich, UK. ISBN:

978-1-904455-17-2.

170. B. Stenuit, L. Eyers, L. Schuler, I. George and S.N. Agathos (2009). Molecular tools for monitoring and validating bioremediation. In: A. Singh, R.C. Kuhad and O.P. Ward, Editors, Advances in Applied Bioremediation, Soil Biology Series Volume 17, pp. 339-353. Springer Verlag, Heidelberg, ISBN: 978-3-540-89620-3.

171. I. George, B. Stenuit and S.N. Agathos (2010). Application of metagenomics to

bioremediation. In: D. Marco, Editor, Metagenomics: Theory, Methods and Applications, pp.

119-140. Caister Academic Press, Norfolk, UK, ISBN: 978-1-904455-54-7.

172. S.N. Agathos (2010). Insect cell culture. In: R.H. Baltz, J.E. Davies and A.L. Demain, Editors, Manual of Industrial Microbiology and Biotechnology, 3

rd Edition,

pp. 212-222.

American Society for Microbiology (ASM) Press, Washington, DC, ISBN: 978-1-

55581-

512-7.

173. M. Pouli and S.N. Agathos (2011). Bioremediation of PAH-contaminated sites: From pathways to bioreactors. In: A.I. Koukkou, Editor, Microbial Bioremediation of Non-metals, pp. 119-147. Caister Academic Press, Norfolk, UK, ISBN: 978-1-904455-83-7.

174. S.N. Agathos (2011). Introduction to Environmental Biotechnology and Safety. In: M. Moo Young, Editor, Comprehensive Biotechnology, Second Edition, Volume 6 - Environmental Biotechnology and Safety, pp. 1-2. Elsevier, Oxford, UK, ISBN: 978-0-44-453352-4.

175. I.F. George, E. Bouhajja and S.N. Agathos (2011). Metagenomics for bioremediation. In: M.

Moo Young, Editor, Comprehensive Biotechnology, Second Edition, Volume 6 - Environmental Biotechnology and Safety, pp. 47-57. Elsevier, Oxford, UK, ISBN: 978-0-44-

453352-4.

176. B.A. Stenuit and S.N. Agathos (2011). Biodegradation and bioremediation of TNT and other nitro explosives. In: M. Moo Young, Editor, Comprehensive Biotechnology, Second Edition, Volume 6 - Environmental Biotechnology and Safety, pp. 167-181. Elsevier, Oxford, UK, ISBN: 978-0-44-453352-4.

177. B. Stenuit and S.N. Agathos (2013). Biodegradation and bioremediation of TNT and other nitro explosives. In: S.A. Elias, Editor, Reference Module in Earth Systems and Environmental Sciences, Elsevier, Amsterdam, Netherlands. ISBN: 978-0-12-409548-9.

C. Peer Reviewed Proceedings

178. B. Volesky and S. Agathos (1974). Oil removal from refinery wastes by air

flotation. Water

Pollution Research in Canada 9: 328-339.

179. S. El Fantroussi, J. Mahillon, H. Naveau and S.N. Agathos (1996). Introduction and PCR Detection of Anaerobic Dechlorinating Bacteria in Soil Slurry Microcosms. Med. Fac.

Landbouww. Univ. Gent 61/4b: 1935-1938.

180. C.M. Peres, H. Naveau and S.N. Agathos (1997). Bioremediation of Nitrobenzene by Simultaneous Reduction of Nitrobenzene into Aniline and Mineralization of the Aniline Formed. 3rd International Symposium on Environmental Biotechnology, Proc. ISEB (H. Verachtert and W. Verstraete, Eds.), Vol. I, pp. 169-172.

181. E. Hellin, H. Ali-Khodja, S. Deseveaux, F. Vandermesse, S.N. Agathos and H. Naveau (1997). A Useful Tool to Study and Optimize the Treatment of VOC in Gaseous Phase: The Tubular Biofilm Reactor. 3rd International Symposium on Environmental Biotechnology. Proc. ISEB (H. Verachtert and W. Verstraete, Eds.), Vol. II, pp. 159-162.

182. B. Van Aken, K. Skubisz, H. Naveau and S.N. Agathos (1997). The Biodegradation of Trinitrotoluene by the White-Rot Basidiomycete Phlebia radiata. 3rd International Symposium on Environmental Biotechnology. Proc. ISEB (H. Verachtert and W. Verstraete, Eds.), Vol. II, pp. 163-167.

183. O. Y. Bitchaeva, S.N. Agathos, C. Ronneau, G. Collard, J. Diaz, C.J. Duggleby, C. Sahut and

J.H. Wissler (1997). Biotechnology for the Nuclear Industry. An International Experience.

3rd International Symposium on Environmental Biotechnology. Proc. ISEB (H. Verachtert and W. Verstraete, Eds.), Vol. I, pp. 295-301.

184. S. Goux, Y. Van Elsen, S.N. Agathos and L. Pussemier (1997). Biodegradation of Three s- Triazine Herbicides in Liquid Media under Different Media Conditions. 49th International

Symposium on Crop Protection. Med. Fac. Landbouww. Univ. Gent 62/2a: 235-242.

185. L. Eyers, B. Stenuit, S. El Fantroussi and S.N. Agathos (2004). Microbial characterization of TNT contaminated soils and anaerobic TNT degradation: high and unusual denitration activity. In: W. Verstraete, Editor, European Symposium on Environmental Biotechnology

ESEB 2004, pp. 51-54, Taylor & Francis Group, London. ISBN 90-5809-653-X

186. E.M.B. Michel, I. Sokolovska and S.N. Agathos (2004). Biodegradation of diesel fuel in soil at low temperature by Rhodococcus erythropolis. In: W. Verstraete, Editor, European Symposium on Environmental Biotechnology ESEB 2004, pp. 843-846, Taylor & Francis Group, London. ISBN 90-5809-653-X

187. D. Roosens, M. Degrez, E.M.B. Michel, S.N. Agathos, C. Dessaucy, A. Van Lierde (2004).

Combined industrial wastewater treatment by association of electrochemical and biological techniques and membrane filtration (MF, UF, NF), Proceedings of the Second National Conference of The Chartered Institution of Water and Environmental Management (CIWEM

2004), Bretton Hall, Wakefield, England, 13-15 September 2004, Edited by N.J. Horan, volume 2, pp 253-260, Leeds, U.K., ISBN: 1-903958-09-01.

188. A. Provost, G. Bastin, S. Agathos and Y.-J. Schneider (2005). Metabolic design of macroscopic models: Application to CHO cells. Proceedings of the 44th IEE Conference on

Decision and Control and the European Control Conference 2005 (ECC-CDC'05), Seville, Spain, December, 12-15, 2005, pp. 2982-2989.

189. S. Sart, Y.-J. Schneider and S.N. Agathos (2009). Impact of growth factors on the proliferation of ear mesenchymal stem cells on porous microcarriers. Proceedings of the 21

st Meeting of the European Society for Animal Cell Technology

(ESACT), Dublin, Ireland, June 7-10, 2009, ESACT Proceedings, Volume 5, Part 2, pp. 161-176.

190. J.-F. Michiels, J. Barbau, S. Sart, S.N. Agathos and Y.-J. Schneider (2009). Effects of soy peptone-supplemented medium on CHO-320 cells. Proceedings of the 21

st

Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009, ESACT Proceedings, Volume 5, Part 5, pp. 471-475.

191. S. Sart, A. Errachid, Y._J. Schneider and S.N. Agathos (2011). Controlled expansion and differentiation of mesenchymal stem cells in a microcarrier based

stirred bioreactor. Proceedings of the 22nd

Meeting of the European Society for Animal Cell Technology (ESACT), Vienna, Austria, May 15-18, 2011, BMC Proceedings 5 (Suppl. 8):P55. Published on line November 22, 2011. http://www.biomedcentral.com/1753-6561/5/S8/P55

192. S. Talvy, R. Nair, P. Demarche, S. Agathos and F. Debaste (2012). Modeling of bisphenol A degradation using immobilized/insolubilized laccase. Proceedings, 20th International Congress of Chemical and Process Engineering, CHISA 2012 and 15th Conference on Process Integration, Modelling and Optimisation for Energy Saving and Pollution Reduction, PRES 2012, Prague, Czech Republic, 25-29 August 2012, 8 pp.

193. B.S. Grama, A. Delhaye, S. Chader, D. Khelifi, S.N. Agathos and C. Jeffryes (2014).

Canthaxanthin, astaxanthin and adonixanthin production from a Dactylococcus microalga in a new flat-plate airlift photobioreactor. Proceedings, 19th National Symposium on Applied

Biological Sciences, Gembloux, Belgium, 7 February 2014. Comm. Appl. Biol. Sci. 79/1: 65-70.

D. Edited Books

194. S.N. Agathos and W. Reineke, Editors (2002). Biotechnology for the Environment: Strategy and Fundamentals. Focus on Biotechnology Series, Volume 3a, Kluwer Academic Publishers, Dordrecht, The Netherlands, 248 pp. ISBN 1-4020-0529-6.

195. S.N. Agathos and W. Reineke, Editors (2002). Biotechnology for the Environment: Soil Remediation. Focus on Biotechnology Series, Volume 3b, Kluwer Academic Publishers, Dordrecht, The Netherlands, 148 pp. ISBN 1-4020-1051-6.

196. S.N. Agathos and W. Reineke, Editors (2003). Biotechnology for the Environment:

Wastewater Treatment and Modeling, Waste Gas Handling. Focus on Biotechnology Series, Volume 3c, Kluwer Academic Publishers, Dordrecht, The Netherlands, 288 pp. ISBN 1-

4020-1131-8.

197. S.N. Agathos, Volume Editor (2011). Environmental Biotechnology and Safety. Volume 6, Comprehensive Biotechnology, Second Edition (M. Moo Young, Editor-in-Chief), Elsevier, Oxford, UK, 955 pp. ISBN: 978-0-44-453352-4.

F. Editorials

198. S. Riva, P. Morandini, G. Sannia and S.N. Agathos (2011). Editorial – Special

issue on International Biotechnology Symposium, IBS-2010: September 14-18, Rimini, Italy.

Enzyme and Microbial Technology 49(6-7): pp. iv.

199. Y. Li, S. Sart and S.N. Agathos (2013). Mass production of stem cell-derived progeny

in bioreactors. International Journal of Stem Cell Research and Transplantation 1(2):1-3.

200. J.M. Park, S.N. Agathos and C.-O. Jeon (2013). Special issue on International Biotechnology Symposium, IBS-2012: September 16-21, Daegu, Korea. Bioresource Technology 145: 1.

201. S.N. Agathos, F. Fava and A. Scoma (2013). Biotechnology for the bio- and green economy. New Biotechnology 30: 581-584.

3. List of patents

Patent Disclosures

1. G.D. Case, M.M. Howton, D.S. Beattie, J.F. Worley III and S.N. Agathos (1989). Method of Detecting Cholinesterase Inhibiting Chemical Agents with Biochemical Amplifying Component in Film Form with a Self-Contained Electronic Membrane Sensor. U.S. Patent and Trademark Office application, November 14, 1989, Serial No. 07/435,765.

2. P. Ledent, H. Michels, S. Agathos, P. Demyttenaere and G. Blackman (1999). Method for

Reducing the Effect of Detergents upon Germination and/or Growth of Microorganisms. European Patent EP 915 061, May 12, 1999. World Patent WO99/20571, April 29, 1999.

3. F. Verhoeye, Y.-J. Schneider, P. Thonart, G. Baré and S. Agathos (2002). Cryoprotective

Solutions. European Patent EP 1 181 865, February 27, 2002.

4. M. Lucas, F. Buchon, B. Gobeau, V. Mertens, S. Vanhulle, S.N. Agathos, C.-M. Bols, A.-M.

Corbisier and D. Wesenberg (2003). Sustainable process for the treatment and detoxification of liquid waste. World Patent WO 03/035581, May 1, 2003.

4. Track records of positions held Oct. 2005 – present Full Professor (“Professeur Ordinaire”), Unit of

Bioengineering, Catholic University of Louvain, Louvain-la-Neuve, Belgium May 1998 – Feb. 2010 Head, Unit of Bioengineering, Catholic University of Louvain, Louvain-la-Neuve, Belgium Oct. 1999- Sept. 2005 Professor, Unit of Bioengineering, Catholic University of Louvain, Louvain-la- Neuve, Belgium Sept. 1993 - Sept. 1999 Associate Professor, Unit of Bioengineering, Catholic University of Louvain, Louvain-la-Neuve, Belgium July 1985 - Aug. 1993 Assistant Professor, Department of Chemical and Biochemical Engineering, and Waksman Institute of Microbiology, Rutgers – The State University of New Jersey, Piscataway, N.J., U.S.A. Feb. 1983 - June 1985 Assistant Professor, Department of Chemical and Biochemical Engineering, University of Western Ontario, London, Canada July 1982 - Feb. 1983 Lecturer, Department of Chemical and Biochemical

Engineering, University of Western Ontario, London, Canada Sept. 1978 - June 1982 Teaching Assistant, Graduate Program in Biochemical Engineering, Department of Applied Biological Sciences (formerly, Department of Nutrition & Food Science), Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, U.S.A. Sept. 1975 - Jan. 1977 Research Assistant, Naval Research Laboratory, Department of Hematology, Boston City Hospital, Boston, Massachusetts, U.S.A. Sept. 1973 - June 1975 Research and Teaching Assistant, Department of Chemical

Engineering, McGill University, Montreal, Canada

5. Fundraising Capacity 1. Towards predictive tools of the dynamics and biodegradation potential of aquatic microbial communities in anthropized watersheds (“DYNAMO”). Collaborative Research Project, National Fund for Scientific Research (FNRS), 2014-2018 (Co-Principal Investigator with P. Servais, ULB),

488,200 € 2. Collection, identification and valorization of microalgae from natural resources. Project n°155419. Wallonia Brussels International (WBI) -- Cooperation with China, Ministry of Science and Technology (MOST), 2014-2015, 17,220 €

3. Integrated Biotechnological Solutions for Combating Marine Oil Spills (“KILL-SPILL”). FP7 EU Project under grant agreement n° 312139 (F7-KBBE), European Commission, 2013-2016, 330,180 € 4. Microbial Resource Management (MRM) in engineered and natural ecosystems (“Micro-Manager”). Inter- University Attraction Pole (IUAP) project n° 27, Belgian Federal Service of Science Policy (BELSPO), 2012-2017, 500,000 € 5. Biomimetic cell and enzyme entrapment. Equipment Grant (Credit to Researchers) for ‘Multisizer’ Coulter particle counter. Project n° 1.5158.12, National Fund for Scientific Research (FNRS), 2012-2014, 39,930 € 6. The Potential of White Rot Fungi Oxidative Enzyme Systems for Removal of Organic Micropollutants in Urban and Industrial Wastewaters – Phase II (“OXEROM 2”). Project n° IP-Envi 10, Program “Impulsion”, Directorate for Research & Innovation (INNOVIRIS), Brussels Capital Region, 2012-2014, 269,710 € 7. Integrated development of hybrid photosynthetic materials by encapsulation of microalgae for the production of high-value metabolites in a photobioreactor (“FOTOBIOMAT”). Project n° 1117323, Walloon Region, Program GREENOMAT, 2011-2015, 444,850 € 8. Oxidative Biocatalysis in a Cartridge (“BIOCATRIDGE”). Program Spin-Off in Brussels (SOIB), Directorate for Research & Innovation (INNOVIRIS), Brussels Capital Region, 2011-2013, 180,080 €; extension 2013-2014, 165,423 € 9. Sustainable Production of Biologically Active Molecules of Marine Based Origin (“BAMMBO”). FP7 EU Project under grant agreement n° 265946 (F7-KBBE), 2011-2014, 456,760 € 10. Microorganism and enzyme immobilization: Novel techniques and approaches for upgraded remediation of underground-, wastewater and soil (“MINOTAURUS”). FP7 EU Project n° F7-KBBE-265946,

European Commission, 2011-2013, 350,400 € 11. Competitiveness and sustainability of Walloon agro-food chains through the establishment of an interdisciplinary platform of expertise for reprocessing their related flows. Walloon Region Grant n° 6089 (“WAL-AID”). Phase II, 2011-2014, 411,413 € 12. BioEnergy from Microalgae (“BEMA”). Project n° 6161, Walloon Region, Competitiveness Cluster TWEED, 2009-2013, 550,825 € 13. Metagenomics for Bioexploration – Tools and Application (“METAEXPLORE”). Project n° FP7-222625- 2, Program “Cooperation”, EU 7th Framework Program, European Commission, 2009-2013, 338,400 € 14. The Potential of White Rot Fungi Oxidative Enzyme Systems for Removal of Organic Micropollutants (“OXEROM”). Project n° IP-Envi 10, Program “Impulsion”, Brussels Capital Region, 2009-2012, 382,851 € 15. Laccase-nanoparticle conjugates for the treatment of wastewater bioreactor and disposal of chemical compounds acting as endocrine disruptors (“LANCE”). Regional support action n° 917011 within the EU funding scheme ERA-NET Matera (FP7), 2009-2011, 142,500 € 16. Competitiveness and sustainability of Walloon agro-food chains through the establishment of an interdisciplinary platform of expertise for reprocessing their related flows. Walloon Region Grant n° 6089 (“WAL-AID”). Phase I, 2009-2011, 114,310 €

17. Research Training for Good European Ground Water Resources (“GOODWATER”). Marie Curie Initial Training Network, Project n° FP7-212683-2, EU 7th Framework Program, European Commission, 2008- 2012, 425,000 € 18. Scaleable Mesenchymal Stem Cell cultivation in Bioreactors, IN-Wallonia-Brussels International Doctoral Fellowship Support (BioWin Cluster), 2008-2011, 75,000 € 19. Efficient Bioreactors for the Production of High Added Value Molecules Based on an Innovative Technology of Implementing Nanoporous Materials (“BIOVAMAT”). Project n° 71/6662, Walloon Region, Program WINNOMAT II, 2008-2011, 311,650 € 20. Insolubilized Oxizymes: Innovative Biocatalysts for Industry and the Environment (“OXINSOL”). Project n°716617, Walloon Region, FIRST Post-Doc, 2008-2010, 175,000 € 21. Innovative Processes for the Bioremediation of Persistent Organic Polutants (POP). Project CFA/VIET/n°1.10. Walloon Region-Brussels Region-French Community of Belgium/Vietnam 2007-2009, 45,000 € 22. Microbial Consortia for the Degradation of Polycyclic Aromatic Hydrocarbons (“CONSOHAP”), contract n° 516144, Walloon Region, FIRST Doctorate (DEI) with Agrostar S.A., 2005-2007, 123,000 €; Extension, 2007-2009, 125,000 € 23. Innovative Agents and Technologies for the Neutralization of Pollutant Mixtures in Soils and Wastewater. Project FC/BUL 2006/00384. Walloon Region-Brussels Region-French Community of Belgium (Cooperation French Community of Belgium – Bulgaria), 2006-2009, 12,900

24. Study of the Genes Involved in the Degradation of Fluorene by Sphingomonas, contract n° 1.5.278.05 (Credit to Researchers). National Fund for Scientific Research (FNRS), 2005-2007, 24,400 € 25. Elimination of the Endocrine Disturbing Substances Nonylphenol, Bisphenol A and Triclosan by Means of Fungal Laccases. Natural Sciences & Engineering Research Council (NSERC, Canada) and Québec Fund for Research on Nature & Technologies (FQRNT) – Doctoral Fellowship Support between Université de Sherbrooke and Université Catholique de Louvain, 2004-2008, 120,000 € 26. Bioengineering of Stem Cells : a Prerequisite for Cell Therapy, Project FSR, Université Catholique de Louvain, 2004-2006, 41,000 € 27. Innovative Process of Remediation of Polycyclic Aromatic Hydrocarbons. Project 04/13586. Walloon Region-Université catholique de Louvain (Cooperation French Community of Belgium – Vietnam), 2005- 2007, 45,000 € 28. Development, Analysis and Testing of New Methods of Supervision and Control for On-Line Optimisation of Fed-Batch Enzyme Production, Grant N° 2.4623.04 for Equipment (Credit to Researchers). National Fund of Scientific Research (FNRS), Belgium, 2004-2005 (Co-Principal Investigator with D. Dochain), 30,000 € 29. Genetic Dissection of the Degradation of Fluorene by Sphingomonas, Fund for the Promotion of Research in Industry and Agriculture (FRIA), Doctoral Fellowship Support, 2003-2007, 96,000 €

30. Microbial Denitration of Nitroaromatic Explosives, Fund for the Promotion of Research in Industry and Agriculture (FRIA), Doctoral Fellowship Support, 2003-2007, 96,000 € 31. Optimisation of Agro-Food Enzyme Production Processes, FSR Project, Université Catholique de Louvain, 2003-2005 (Co-Principal Investigator with D. Dochain), 42,500 € 32. FELBIPUR – Integrated Cleanup of Recalcitrant Industrial Effluents by Electrochemical, Tangential Filtration and Biological Means. Directorate General of Technology, Research and Energy (DGTRE), Grant N° 215168. Walloon Region-Université Catholique de Louvain-Université Libre de Bruxelles, 2003-2005, 210,205 € 33. ENVIROFELLOWS – Short Term Exchanges of Early Career Scientists in Environmental Biotechnology between the European Union and the United States (Partners: All EU member countries). CEE N° QLAM- 2001-00292. Programme Quality of Life, EU 5th Framework, European Commission, 2002-2004, 150,000 € 34. MADOX – Molecular & Engineering Approach to the Anaerobic Degradation of Organic Xenobiotics (Partners: Belgium, France, Germany, Spain). CEE N° QLRT-2001-00345. Coordinator & PI. Program Quality of Life, EU 5th Framework, European Commission, 2002-2005, 1,755,200 € 35. Study of the Physiology of Pseudomonas, Azoarcus anaerobius and Thauera aromatica in the Anaerobic Biodegradation of Amino- and Nitroaromatics. Large Equipment Grant (Credit to Researchers). National Fund of Scientific Research (FNRS), Belgium, 2001-2002, 11,155 € 36. Innovative Process of Remediation of Polycyclic Aromatic Hydrocarbons: the Hybrid Biological – Electrochemical Approach. Project 01/11818. Walloon Region-Université Catholique de Louvain (Cooperation French Community of Belgium – Vietnam), 2001-2004, 8,180 € 37. Denitrification of Water in Bioreactor Assisted by an Electic Field. Project 01/11981. Walloon Region- Université catholique de Louvain (Cooperation French Community of Belgium – Bulgaria), 2001-2003,17,055 € 38. Bioengineering of Stem Cells. FSR Project, Université Catholique de Louvain, 2002-2004, 60,734 € 39. OPTICELL – Development of Cell Culture Systems Optimized and Made Safe for the Bioprocess Industries. Walloon Region, Grant N° 3677 FIRST SPIN-OFF, 2000-2002 (Co-Principal Investigator with Y.-J. Schneider), 54,536 €. 40. EASYFORM – Interdisciplinary Integration and Valorisation of Materials Moldable at Low Melting Point. Directorate General of Technology, Research and Energy (DGTRE), Grant N° 14585. Walloon Region- Université Catholique de Louvain-Université de Liège-Université Mons-Hainaut-Université Libre de Bruxelles, 2000-2004, 204,041 € 41. Development, Optimisation and Characterisation of a Multifunctional Permeable Barrier Technology for the Removal of Selected Pollutants. Grant N° 99-57 UCL-VITO from Vlaamse Instelling voor Technologisch Onderzoek (VITO), 1999-2003, 99,157 € 42. Improvement of the Overall Functioning of Urban and Industrial Wastewater Treatment Plants: Impact of a Solubilisation Treatment Using Enzyme-Bacteria Complexes. Walloon Region, Grant N° 3957 FIRST- Entreprise (Industrial Partner: REALCO), 2000-2002, 69,696 €

43. New System of Glycoprotein Production in Perfused Bioreactor at High Cell Density.

Directorate General of Technology, Research and Energy (DGTRE), Grant N° 981/3711. Walloon Region-Université Catholique de Louvain-Université de Liège, 1999-2002, 265,081 €

44. Psychrophilic Microorganisms, Tools of Choice for Bioremediation. Directorate General of Technology, Research and Energy (DGTRE), Grant N° 991/4213. Walloon Region-Université Catholique de Louvain- Université de Liège, 1999-2002, 164,973 € 45. Innovative Process of Biodegradation of Industrial Dyes Using Fungal Strains. Directorate General of Technology, Research and Energy (DGTRE), Grant N° 981/3870. Walloon Region-Université Catholique de Louvain-Université Libre de Bruxelles, 1999-2002, 396,357 € 46. Efficient Production of Proteins in Bioreactor: Impact and Control of Oxygen and Carbon Dioxide in the Cell-Baculovirus System. FSR Project, Université Catholique de Louvain, 1999-2001, 48,339 € 47. Development and Theoretical Analysis of Supervision and Control Methods of Discrete Parameter Systems. Development and Experimental Validation on a Biological Wastewater Treatment Process. Large Equipment Grant (Fund of Collective Fundamental Research, FRFC). National Fund of Scientific Research (FNRS), Belgium, 1998-2001 (Co-Principal Investigator with D. Dochain), 98,166 € 48. Biological Wastewater Treatment (Reduction of Sludge Volume). Directorate General of Technology, Research and Energy (DGTRE), Grant N° 981/3800. Walloon Region-Université Catholique de Louvain- Université de Liège, 1998-2001, 328,459 € 49. CHLOREM – Demonstration Project: In Situ Bioremediation of Soil Contaminated with Chlorinated Ethenes and Nickel, Demonstration of Cleanup by Anaerobic Bacteria (Partners: Belgium, Germany, The Netherlands). CEE n° BIO4-CT98-0303. Programme BIOTECH, EU 4th Framework, European Commission, 1998-2001, 200,000 € 50. Wastewater Treatment Using Enzyme-Bacterial Complexes. Walloon Region, Industry-University Contract N° 3599 (Industrial Partner: REALCO), 1998-2000, 154,834 € 51. Elimination of N-Glycolylneuraminic Acid Residues in Recombinant Glycoproteins. Animal Cell Factories RTD Project (Partners: Belgium, France, Italy, United Kingdom). Grant N° BIO4-CT96-0767. Programme BIOTECH, EU 4th Framework, European Commission DG XII, 1997-2000, 296,000 € 52. Biological Pre-Treatment of Sewage: an Innovative Technology to Improve the Efficiency of Wastewater Treatment Plants. Walloon Region, Programme FIRST N° 3180 (Industrial Partner: REALCO), 1996-1999, 183,864 € 53. Efficient Production of Proteins by Insect Cells in Perfusion Reactor at High Cell Density. FDS Project, Université Catholique de Louvain, 1996-98, 56,024 €. 54. Enhanced Biodegradation of Nitroaromatic Compounds. Large Equipment Grant (Credit to Researchers). National Fund of Scientific Research (FNRS) - Loterie Nationale, Belgium, 1994-1995, 29,747 €. 55. Bioremediation of Nitroaromatic Pollutants. FDS Project, Université Catholique de Louvain, 1993-1995,60,244 €. 56. Biodegradation of Nitro-Propellants. US Army, 1992 – 1993, $61,500 (general support) plus $259,000 (equipment). 57. Antibiotic Fermentation. Bryan Cave, New York 1992-1993, $18,200 58. Analytical Equipment Grant. Hoffmann LaRoche, Inc., New Jersey, 1993, $60,000 59. Bioreactor Cultivation of Insect Cells. Schering-Plough Research Institute, New Jersey, 1989-1993, $120,000 60. Bioprocess Engineering for Heterologous Protein Production by Yeast. National Science Foundation (NSF), 1989 – 1992, $100,000

61. Enzyme Stabilization for Biosensor Development. Resource Technologies Group, Inc., West Virginia, subcontract of US Air Force, 1989 – 1992, $25,000 62. Bioreactor Culture of Insect Cells. US Public Health Service, 1987 – 1988, $5,000 63. Bioreactor Culture of Insect Cells. Rutgers University Research Council, 1987 – 1989, $10,000 64. Bioprocess Development for Cyclosporin Production. National Science Foundation (NSF) Career Initiation Award, 1987 – 1989, $60,000 65. Bioprocess Development for Immunosuppressant Elaboration. US Public Health Service, 1986 – 1987, $8,900 66. Cyclosporin Production by Free and Immobilized Cells. Rutgers University Research Council, 1985 – 1987, $5,100 67. Gramicidin Synthetase Stabilization. Natural Sciences and Engineering Research Council (NSERC), Canada, 1983 – 1985, $54,000

6. Honors and awards Elected Fellow, American Institute for Medical and Biological Engineering, 2014 (Honor conferred at AIMBE Annual Event, US National Academy of Sciences, Washington, DC) Keynote Lecture Award, 13

th International Biotechnology Symposium (IBS2008),

Dalian, China, 2008 Opening Keynote Lecture, 1

st (Inaugural) Symposium of Hellenic Scientific Society

MicroBioKosmos, Athens, 2008 Elected Fellow, Society for Industrial Microbiology & Biotechnology, 2006 (Honor conferred at Annual Meeting, SIMB, Baltimore, Maryland) Best Paper Award 2006, Bioprocess & Biosystems Engineering journal of Springer Verlag Keynote Lecturer on Molecular Engineering and Biology of Invertebrate Cell Cultures, 11th International Conference on Invertebrate Cell & Tissue Culture, 2004 World Congress on In Vitro Biology, San Francisco, California, 2004 Plenary Lecture Award, International Conference on Bioremediation of Soil and Groundwater, Cracow, Poland, 2004 Foreign Orator in Honour of the CNRS Silver Medal Award to Dr. Annie Marc, Institut National Polytechnique de Lorraine, Nancy, France, 2003 Plenary Lecture & Topic Coordinator Award, 6

th International Symposium on Environmental

Biotechnology (ISEB 2002), Veracruz, Mexico, 2002 Keynote Lecture Award, First European Bioremediation Conference, Chania, Greece, 2001 National Research and Development Council (CNPq) of Brazil Visiting Professorship Award, 1992 Merit Award from the President of Rutgers – The State University of New Jersey “in Recognition of Service to the University”, 1988 Research Career Initiation Award at Rutgers – The State University of New Jersey, National Science Foundation, 1987 NATO Science Fellowship at MIT, 1978-1981 Sigma Xi, MIT Chapter, 1976; Rutgers Chapter, 1987 State Scholarship, Greek Scholarship Foundation (IKY), 1969-1973